EFFECT OF CHOLINE IN ALL-CONCENTRATE DIETS OF FEEDLOT STEERS AND ON RUMINAL ACIDOSIS

1985 ◽  
Vol 65 (1) ◽  
pp. 135-146 ◽  
Author(s):  
T. S. RUMSEY

Four trials with beef steers were conducted to determine if choline supplementation of an all-concentrate diet improved performance and affected ruminal lactic acidosis. Trial 1 was a preliminary feeding trial with 12 individually fed steers averaging 356 kg initial body weight. Trials 2 and 3 were feedlot trials with 64 and 80 steers averaging 359 and 320 kg initial body weights, respectively. Trial 4 was an intensive trial with three ruminal-fistulated steers in a 3 × 3 Latin square design in which subacute ruminal acidosis was produced. Choline supplementation neither consistently affected feedlot performance and carcass merit measurements in the feeding trials, nor consistently affected end products of ruminal fermentation. Choline appeared to reduce total plasma amino acids and increase plasma urea (measurements made in Trials 2 and 3) and reduce serum glucose, triglycerides and glutamic pyruvic transaminase while increasing serum total lipids and cholesterol (measurements made in Trial 3). Choline did not affect ruminal volatile fatty acid, pH, ammonia and lactic acid isomer changes associated with subacute lactic acidosis. The results indicate that the levels of choline supplementation of an all-concentrate diet for beef steers used in this study did not affect ruminal acidosis and did not improve performance of feedlot steers. Key words: Choline, acidosis, lactic acid, rumen, steer, beef cattle

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 919
Author(s):  
Verónica M. Merino ◽  
Lorena Leichtle ◽  
Oscar A. Balocchi ◽  
Francisco Lanuza ◽  
Julián Parga ◽  
...  

The aim was to determine the effect of the herbage allowance (HA) and supplement type (ST) on dry matter intake (DMI), milk production and composition, grazing behavior, rumen function, and blood metabolites of grazing dairy cows in the spring season. Experiment I: 64 Holstein Friesian dairy cows were distributed in a factorial design that tested two levels of daily HA (20 and 30 kg of dry matter (DM) per cow) and two ST (high moisture maize (HMM) and cracked wheat (CW)) distributed in two daily rations (3.5 kg DM/cow/day). Experiment II: four mid-lactation rumen cannulated cows, supplemented with either HMM or CW and managed with the two HAs, were distributed in a Latin square design of 4 × 4, for four 14-d periods to assess ruminal fermentation parameters. HA had no effect on milk production (averaging 23.6 kg/day) or milk fat and protein production (823 g/day and 800 g/day, respectively). Cows supplemented with CW had greater protein concentration (+1.2 g/kg). Herbage DMI averaged 14.17 kg DM/cow.day and total DMI averaged 17.67 kg DM/cow.day and did not differ between treatments. Grazing behavior activities (grazing, rumination, and idling times) and body condition score (BCS) were not affected by HA or ST. Milk and plasma urea concentration increased under the high HA (+0.68 mmol/L and +0.90 mmol/L, respectively). Cows supplemented with HMM had lower milk and plasma urea concentrations (0.72 mmol/L and 0.76 mmol/L less, respectively) and tended (p = 0.054) to have higher plasma β-hydroxybutyrate. Ruminal parameters did not differ between treatments.


2008 ◽  
Vol 88 (4) ◽  
pp. 685-692
Author(s):  
D. R. Ouellet ◽  
L. Faucitano ◽  
D. Pellerin ◽  
M. D’Amours ◽  
R. Berthiaume

Two experiments were conducted to determine the relationship between corn particle size and soybean meal treatment on growth, diet digestibility, and nitrogen balance of growing steers. In the first experiment, 40 medium-frame beef steers (250 ± 11 kg) were fed individually for 140 d a diet based on grass silage offered for ad libitum consumption and supplemented with either 3.5 kg of DM d-1 of cracked corn (CC) or ground corn (GC) and with 450 g of DM d-1 of solvent extracted (SS) or lignosulfonate-treated soybean meal (Soypass™ SP). Dry matter intake was not affected by treatments and averaged 8.6 ± 0.3 kg d-1 (P > 0.10). Average daily gain was higher for animals receiving the ground corn than those fed cracked corn. Feed to gain ratio was not affected by treatments. There was an interaction between treatments for plasma urea-N concentration, with source of soybean meal having no effect with CC. When compared with SS, SP supplement reduced plasma urea-N when fed with GC. No effect of soybean meal and its interaction with corn processing was observed on growth performance. In the second experiment four additional steers were used in a 4 × 4 Latin square design to evaluate diet digestibility and nitrogen balance. There was an interaction between treatments for starch apparent digestibility and N retained, the values being respectively, 90, 86, 93, and 92% (SEM = 1.2; P < 0.01) and 36, 42, 44 and 41 g d-1 (SEM = 2.8; P < 0.03) for CCSS, CCSP, GCSS and GCSP, respectively. Altogether, the results indicate a slight advantage to reduce particle size of corn in growing steers fed grass silage. However, soybean meal treatment resulted in limited effects on growth and digestion. Key words: Rumen carbohydrate, undegradable protein, performance, steers


Author(s):  
Catherine L Lockard ◽  
Caleb G Lockard ◽  
Wyatt N Smith ◽  
Kendall J Karr ◽  
Ben P Holland ◽  
...  

Abstract Six ruminally cannulated steers (average BW = 791 + 71 kg) were used in a replicated 3 × 3 Latin square experiment to determine the effects of roughage type on rumination, fiber mat characteristics, and rumen fermentation variables. Three roughages were included at 7% (DM basis) in a steam flaked corn-based diet; cotton burrs (CB), wheat silage (WS), or corn stalks (CS). Steers were fitted with a sensory collar to record rumination behaviors in 2-h intervals at the beginning of the experiment. Each 30-d period consisted of a 7-d of recovery, 14-d of diet adaptation, 7-d of rumination data collection (daily and bi-hourly average rumination), 1-d of rumen fluid collection, and 1-d of rumen evacuations. In situ degradation of individual roughages was determined for 4-d after period 3 evacuations. During rumen evacuations, ruminal contents were removed; the rumen fiber mat (RF) was separated from the liquid portion with a 2 mm sieve, weighed, and a subsample was dried. Data were analyzed using the MIXED procedure of SAS with steer as the experimental unit and roughage (CB, WS, and CS) as the main effect. Dry matter intake (DMI) was not different for CB and WS (P = 0.25) and greatest for steers consuming CS diet (P  &lt; 0.01). Roughage type did not influence the weight of the RF dry matter (%; DM; P = 0.92), RF weight (P = 0.69), or RF:DMI ratio (P = 0.29). Daily rumination (min/d) did not differ among roughages (P = 0.40), but min of rumination/kg of DMI was greatest for CS (18.0 min), min/kg of NDF was greatest for WS (89.8 min; P = 0.02), and min/kg of peNDF was greatest for CS (132.4 min; P  &lt; 0.01). Wheat silage had the greatest percentage of soluble DM and CB-R and CS-R (P  &lt; 0.01) had the greatest ruminal degraded DM fraction. Rumen fiber mat did not differ for roughages, although rumination min/kg of DMI and peNDF was greatest for steers consuming CS and WS. In situ degradation determined that CB-R and CS-R had the greatest percentage of ruminal degraded DM. Based on the objective of the experiment, roughage type did not influence daily rumination or fiber mat characteristics.


2020 ◽  
Vol 100 (4) ◽  
pp. 716-728
Author(s):  
A.A. Pursley ◽  
B. Biligetu ◽  
T.D. Warkentin ◽  
H.A. Lardner ◽  
G.B. Penner

The objective of this study was to evaluate the inclusion rate of pea hay in barley or oat hay diets for beef cattle. Six ruminally cannulated heifers (407 ± 38 kg) were used in a 6 × 6 Latin square (25 d periods) with a 2 × 3 factorial design. Treatments included whole-crop barley or oat hay with pea hay blended in to achieve inclusion rates of 0%, 15%, or 30% (dry matter basis) of the forage. Pea hay inclusion increased dry matter intake (DMI; P = 0.03) by 0.75 kg d−1 relative to diets without pea hay, but the response was not linear or quadratic. Inclusion of pea hay linearly increased mean ruminal pH (P = 0.039), the concentration of butyrate in ruminal fluid (P = 0.013), plasma urea nitrogen (N) concentration (P = 0.001), and quadratically increased ruminal ammonia concentration (P < 0.001). Pea hay inclusion reduced crude protein (CP) digestibility by 2.87% relative to cereal-only treatments (P = 0.025), but did not affect N intake, microbial N, or N excretion. Overall, pea hay inclusion increased DMI, increased ruminal butyrate concentration, but reduced CP digestibility without affecting N balance.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 331-332
Author(s):  
Iorrano A Cidrini ◽  
Laura Prados ◽  
Willian Foresto ◽  
Eduarda Mariano ◽  
William Souza ◽  
...  

Abstract The objective of this experiment was to evaluate the effect of cattle supplementation (3g/kg BW) with urea (U) and tannin extracts (TE; SilvaFeed Bypro®️) during the dry season on performance, ruminal fermentation and blood parameters. Trial 1-Sixty-four Nellore (294 ± 15.2 kg BW) were used to determine the effect of supplementation during the growing phase (137d) on performance. The animals were blocked by BW to one of four treatments: 1) 30g urea/kg DM supplement (LU); 2) 30g urea/kg DM supplement + 7g TE/kg DM supplement (LU+TE); 3) 50g urea/kg DM supplement (HU); 4) 50g urea/kg DM supplement + 7g TE/kg DM supplement (HU+TE). Trial 2- Eight ruminally cannulated Nellore fed with trial 1 diet (4 x 4 Latin square) was used to evaluate DM intake and digestibility, ruminal pH, VFA, ruminal ammonia, serum glucose and urea at different times after feeding. The data were analyzed by ANOVA using PROC MIXED, SAS 9.4. There was no interaction (P &gt; 0.10) between U and TE, the treatments did not affect (P &gt; 0.10) ADG, intake or digestibility, VFA total, serum glucose and urea (Table1). However, animals fed TE showed butyrate (Figure 1) tended to higher on time 12-18h (Time × TE; P = 0.06), while the acetate: propionate ratio tended (Time × TE; P = 0.08) to decrease. The isovalerate tended to be higher on time 6h in animals fed HU+TE while LU+TE tended to be higher on time 12-18h. The ruminal pH of the animals fed with LU tended to be lower on time 6h compared to HU, while ruminal ammonia was higher (P &lt; 0.01) to animals fed HU on time 6h compared to LU. Overall, TE and U did not influence the performance of Nellore and the inclusion of TE tended to improve the efficiency in ruminal fermentation.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 438-439
Author(s):  
Joel D Sugg ◽  
Carly A Hoffmann ◽  
Lucas B Kondratovich

Abstract The effects of hay type and protein supplementation on intake, feeding behavior, nutrient digestion, and ruminal digestion characteristics were evaluated. Ruminally cannulated Angus beef steers (n = 6; BW = 304 kg ± 11 kg) were randomly assigned within a sequence of treatments using a 4 × 6 unbalanced Latin square design (6 steers; and 4 diets; fed once-daily). A 2 x 2 factorial treatment arrangement was used as follows: 1) ‘WW-B. Dahl’ Old World bluestem [Bothriochloa bladhii (Retz) S.T. Blake; WWBD or Eragrostis tef (Zucc.) Trotter; TEFF); and 2) dried distillers grain (DDGS) at 0 or 0.5% BW. Each period consisted of a 14-d of adaptation and 7-d collection. Steers were observed (5-min intervals, 24 h) for behavioral assessment; while ruminal pH was continuously measured (wireless pH probe), and ruminal fluid collected at 0, 2, 4, 8, and 16 h after-feeding. Steers fed TEFF hay and those fed DDGS (both, P = 0.04) had greater DMI compared to WWBD and not supplemented. Chewing activity did not differ (P ≥ 0.54). Non-supplemented steers spent more time eating hay (P &lt; 0.01) than supplemented steers. Average ruminal pH of TEFF (6.32) was lower (P &gt; 0.01) than WWBD (6.56). Non-supplemented steers produced less in vitro total gas and methane (both, P = 0.02) per g rumen fluid DM. The VFA profile was not affected (P ≥ 0.45) by treatments. Apparent total-tract digestibility and ruminal degradation (P ≤ 0.01) were greater with TEFF fed steers than WWBD. Hemicellulose digestion was reduced by 6.95% (P = 0.03) with DDGS supplementation. An annual hay in place of a conventional perennial hay improved intake, ruminal digestion of nutrients, without affecting feeding behavior, while supplementation with DDGS reduced forage intake time and quantity, without negatively affecting ruminal fiber digestion.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 315-316
Author(s):  
Iorrano A Cidrini ◽  
Laura Prados ◽  
Willian Foresto ◽  
Eduarda Mariano ◽  
William Souza ◽  
...  

Abstract The objective of this experiment was to evaluate the effect of cattle supplementation (3g/kg BW) with urea (U) and tannin extracts (TE; SilvaFeed Bypro®️) during the dry season on performance, ruminal fermentation and blood parameters. Trial 1-Sixty-four Nellore (294 ± 15.2 kg BW) were used to determine the effect of supplementation during the growing phase (137d) on performance. The animals were blocked by BW to one of four treatments: 1) 30g urea/kg DM supplement (LU); 2) 30g urea/kg DM supplement + 7g TE/kg DM supplement (LU+TE); 3) 50g urea/kg DM supplement (HU); 4) 50g urea/kg DM supplement + 7g TE/kg DM supplement (HU+TE). Trial 2- Eight ruminally cannulated Nellore fed with trial 1 diet (4 x 4 Latin square) was used to evaluate DM intake and digestibility, ruminal pH, VFA, ruminal ammonia, serum glucose and urea at different times after feeding. The data were analyzed by ANOVA using PROC MIXED, SAS 9.4. There was no interaction (P &gt; 0.10) between U and TE, the treatments did not affect (P &gt; 0.10) ADG, intake or digestibility, VFA total, serum glucose and urea (Table1). However, animals fed TE showed butyrate (Figure 1) tended to higher on time 12-18h (Time × TE; P = 0.06), while the acetate: propionate ratio tended (Time × TE; P = 0.08) to decrease. The isovalerate tended to be higher on time 6h in animals fed HU+TE while LU+TE tended to be higher on time 12-18h. The ruminal pH of the animals fed with LU tended to be lower on time 6h compared to HU, while ruminal ammonia was higher (P &lt; 0.01) to animals fed HU on time 6h compared to LU. Overall, TE and U did not influence the performance of Nellore and the inclusion of TE tended to improve the efficiency in ruminal fermentation.


2000 ◽  
Vol 80 (1) ◽  
pp. 161-168 ◽  
Author(s):  
S. J. Pylot ◽  
J. J. McKinnon ◽  
T. A. McAllister ◽  
A. F. Mustafa ◽  
J. Popp ◽  
...  

Two experiments were conducted to determine the feeding value of canola screenings in combination with barley grain for beef steers. Four dietary treatments were used. These included canola screenings:barley grain ratios of 100:0; 75:25, 50:50, and 25:75 (as-fed basis). In a metabolic trial, the effects of dietary treatment on ruminal fermentation parameters were determined in a 4 × 4 Latin square design experiment using four ruminally fistulated steers. In a production trial, 66 individually fed steers were used in an 83-d finishing trial to determine the performance and carcass characteristics of feedlot cattle fed different levels of canola screenings. A barley grain/barley silage-based control diet was also fed for comparison purposes. Ruminal pH decreased (P < 0.05) while total volatile fatty acid concentrations increased (P < 0.05) as the level of barley grain in the diet increased. Ruminal NH3–N followed a pattern (P < 0.05) similar to that of pH. Inclusion level of canola screenings had no effect on DM intake. However, ADG and feed efficiency increased (P < 0.05) as the level of barley grain in the diet increased. Increasing the level of barley grain in the finishing diet decreased (P < 0.05) lean meat yield and increased (P < 0.05) carcass fat. It was concluded that canola screenings can be included as a source of fiber in barley-based diets. However, levels in excess of 500 g kg−1 reduced the performance of feedlot steers. Performance and cost of gain with 250 and 500 g kg−1 canola screenings in barley-based finishing diets were comparable with those fed an 800 g kg−1 barley grain and 200 g kg−1 barley silage diet. Key words: Canola screenings, ruminal fermentation, feedlot steer performance


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 166-166
Author(s):  
Kaitlyn N Pierce ◽  
Andrew P Foote ◽  
Paul A Beck ◽  
Colton A Robison ◽  
Andrea L Warner ◽  
...  

Abstract Finishing cattle are fed high concentrate diets to increase deposition of both muscle and adipose tissue and decrease cost of gain. The objective of this experiment was to evaluate the effects of increased roughage inclusion late in the finishing period on growth performance, carcass traits, and ruminal and fecal characteristics of feedlot steers. Treatments included a control (CON; 6% roughage DM), intermediate (INT; 12% roughage DM), and high (HGH; 18% roughage DM) roughage diets. Crossbred beef steers (n = 54; BW = 289 ± 35.6 kg) were assigned to treatments for the late finishing period in a randomized complete block design (4 pens of INT and HGH, 5 pens of CON; 4 steers per pen). Experimental diets contained prairie hay, Sweet Bran, rolled corn, dry supplement, urea, and a corn steep and molasses-based liquid supplement. The inclusion rate of roughage and rolled corn were adjusted for each treatment diet. Steers in HGH tended to have increased overall DMI (P = 0.07). No differences in final BW, overall ADG, or gain:feed were observed among treatments (P ≥ 0.72). Steers fed HGH had the greatest REA (P = 0.03) and a tendency for a lower yield grade (P = 0.08). Fat thickness, HCW, marbling, liver scores, and KPH did not differ (P ≥ 0.29) among treatments. Steers fed the HGH diet had a lower fecal pH at the end of finishing (P = 0.05) compared to CON and INT steers. Ruminal lactate was increased on d 14 for CON steers compared to other treatments (P &lt; 0.001). No differences were observed for ruminal pH (P ≥ 0.11). Results of this experiment suggest that increasing roughage late in the finishing period does not negatively impact growth performance or carcass characteristics, but may alter ruminal fermentation and post ruminal digestion.


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