COMPOSITION, AMINO ACID DIGESTIBILITIES AND PROTEIN QUALIT OF HIGH-LYSINE BARLEY LINES FOR GROWING RATS

1982 ◽  
Vol 62 (4) ◽  
pp. 1149-1159
Author(s):  
R. MISIR ◽  
W. C. SAUER
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Lysine Content ◽  
Analysis Method ◽  
Fecal Analysis ◽  
Protein Sources ◽  
High Lysine ◽  
Growing Rats ◽  
Hiproly Barley ◽  
Similar Soil

Hiproly barley and five experimental barley lines bred and grown in Alberta under similar soil and environmental conditions were evaluated as sole protein sources in two rat trials. In exp. 1, compared to Gait barley (control), Hiproly and the test lines (Lines 1, 2 and 3) contained 9–22% more nitrogen (N). Hiproly and Line 1 also contained 21 and 29% more lysine, respectively. Consequently, Line 1 could be classified as a "Hiproly" barley, Line 2 as "high protein" barley and Line 3 a "normal" barley. Determined by the fecal analysis method, lysine was least digestible of the amino acids (AA). Apparent lysine digestibilities were similar for all the barleys. In exp. 2, compared to Gait barley (control), Lines 4 and 5 were similar in N content, but contained 13 and 7.5% more lysine, respectively. Measured by the relative protein value method, there were no differences in performance, reflecting the small differences in lysine content among the barleys. The apparent N digestibilities in barley were 10–11 percentage units lower (P < 0.01) than in casein. Calculated chemical scores showed that among the barleys only Line 1 might adequately provide all the AA, including lysine, for 60- to 100-kg pigs. Key words: High lysine barleys, hiproly, barley lines, apparent digestibilities, relative protein values, rats

AVAILABILITIES OF AMINO ACIDS IN BARLEY AND WHEAT FOR GROWING PIGS

1981 ◽  
Vol 61 (3) ◽  
pp. 793-802 ◽  
Author(s):  
W. C. SAUER ◽  
J. J. KENNELLY ◽  
F. X. AHERNE ◽  
R. M. CICHON
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Growing Pigs ◽  
Chromic Oxide ◽  
Analysis Method ◽  
Fecal Analysis ◽  
Amino Acid Levels

The apparent ileal and fecal availabilities of amino acids in four barley samples and in four wheat samples were determined with four growing barrows (Yorkshire × Lacombe), fitted with ileocecal re-entrant cannulae. The experimental rations consisted of 96.8% barley or wheat, 2.7% minerals plus vitamins and 0.5% chromic oxide. True availabilities were calculated based on metabolic ileal and fecal amino acid levels obtained in previous studies. There were differences (P <.05) between the apparent ileal as well as between the true ileal availabilities of lysine, methionine, phenylalanine and glutamic acid in the barley samples. The apparent ileal availabilities ranged from 64.9 to 72.5% for lysine, from 72.1 to 83.9% for methionine and from 64.4 to70.4% for threonine. In the same sequence, the true availabilities of these amino acids ranged from 71.2 to 78.8%, from 75.4 to 87.3% and from 74.6 to 79.3%. There were no differences between the apparent as well as between the true availabilities of amino acids in the barley samples when these were measured by the fecal analysis method. With the exception of serine, there were no differences between the apparent as well as between the true ileal availabilities of amino acids in the wheat samples. The average apparent ileal availabilities were 76.7, 82.1 and 76.5% for lysine, methionine and threonine, respectively. In the same sequence, the average true ileal availabilities of these amino acids were 83.5, 85.0 and 85.7%.

APPARENT AVAILABILITIES OF AMINO ACIDS IN CORN, WHEAT AND BARLEY FOR GROWING PIGS

1977 ◽  
Vol 57 (3) ◽  
pp. 585-597 ◽  
Author(s):  
W. C. SAUER ◽  
S. C. STOTHERS ◽  
G. D. PHILLIPS
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Growing Pigs ◽  
The Other ◽  
Cereal Grains ◽  
Analysis Method ◽  
Fecal Analysis ◽  
Other Hand ◽  
Indispensable Amino Acids

The apparent ileal and fecal recoveries of amino acids in corn, wheat and barley were determined with barrows ranging in weight from 50 to 70 kg that were fitted with ileocecal re-entrant cannulas. Average ileal availabilities were 4–7% less than average fecal availabilities in the three cereal grains. Of the indispensable amino acids in the cereal grains, the recoveries of lysine and threonine were approximately equal in being the lowest at the end of the ileum, and they decreased from those in corn, to wheat and to those in barley. The recovery of lysine was the lowest in all cereal grains when determined by the fecal analysis method. The apparent fecal recoveries of amino acids in the cereal grains obtained from cannulated pigs were essentially similar to those obtained from non-cannulated pigs. Apparent ileal and fecal recoveries of amino acids in finely ground and cracked wheat were determined with eight barrows that weighed approximately 75 kg. The ileal recoveries of most amino acids were significantly higher (P <.05) in finely ground than in cracked wheat. On the other hand, differences in amino acid availabilities due to processing of wheat could not be detected by the use of the fecal analysis method.

scholarly journals AVAILABILITY OF AMINO ACIDS FROM BARLEY, WHEAT, TRITICALE, AND SOYBEAN MEAL FOR GROWING PIGS

1974 ◽  
Vol 54 (1) ◽  
pp. 97-105 ◽  
Author(s):  
W. C. SAUER ◽  
P. M. GIOVANNETTI ◽  
S. C. STOTHERS
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Soybean Meal ◽  
Growing Pigs ◽  
Cereal Grains ◽  
Available P ◽  
Analysis Method ◽  
Fecal Analysis

Amino acid (AA) availabilities from barley, wheat, triticale and soybean meal for barrows weighing 10 and 30 kg were determined by the fecal analysis method. In general, the true availability of each essential AA decreased in the following order: soybean meal > triticale > wheat > barley. Lysine was the least available (P < 0.05) essential AA from the cereal grains. When pigs were fed either triticale, wheat or barley, the respective lysine availabilities were 86, 81 and 77% (10-kg barrows) and 78, 67 and 65% (30-kg barrows). Availabilities of isoleucine, methionine, threonine and valine were similar and were grouped as the next least available essential AA from the cereal grains. Their availabilities varied between 90–92, 87–90 and 85–88% for the 10-kg pigs and between 87–88, 85–86 and 82–85% for the 30-kg pigs fed triticale, wheat and barley, respectively. Methionine was the least available essential AA (86.3%) from soybean meal for the 30-kg barrows. However, the availability of methionine was not significantly different (P < 0.05) from those of lysine, threonine and valine.

EFFECT OF VARIETY AND GROWING CONDITIONS ON THE PROTEIN AND AMINO ACID COMPOSITION OF RAPESEED

1963 ◽  
Vol 43 (1) ◽  
pp. 65-67 ◽  
Author(s):  
D. R. Clandinin ◽  
Louise Bayly
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Protein Content ◽  
Essential Amino Acids ◽  
Lysine Content ◽  
Amino Acid Distribution ◽  
Cent Level ◽  
Growing Conditions ◽  
Polish Variety

The protein content and the distribution of essential amino acids in the protein of six varieties or strains of rapeseed grown at three widely separated locations in Alberta, Beaverlodge, Edmonton and Lethbridge, were studied. Differences in protein content and amino acid distribution in the protein of the seed attributable to varietal or strain effects were noted. Turkish and Polish rapeseed were significantly lower in protein content than rapeseed of the Argentine variety. The Argentine variety and the two strains of Argentine-type studied were significantly lower in lysine than the Polish variety. Station differences in protein content approached significance at the 5 per cent level while location had a highly significant effect on the lysine content of the protein of the seed and significant effects on the histidine, arginine, phenylalanine and leucine content.

scholarly journals Macronutrient composition, true metabolizable energy and amino acid digestibility, and indispensable amino acid scoring of pulse ingredients for use in canine and feline diets

2020 ◽  
Vol 98 (6) ◽  
Author(s):  
Lauren M Reilly ◽  
Patrick C von Schaumburg ◽  
Jolene M Hoke ◽  
Gary M Davenport ◽  
Pamela L Utterback ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Dry Matter ◽  
Protein Quality ◽  
Metabolizable Energy ◽  
Macronutrient Composition ◽  
Total Dietary Fiber ◽  
Protein Sources ◽  
Indispensable Amino Acid ◽  
Limiting Amino Acid

Abstract The rising consumer demand for alternative and sustainable protein sources drives the popularity of the use of plant-based proteins in the pet food industry. Pulse crops, which include beans, peas, lentils, and chickpeas, have become an important addition to both human and animal diets due to their protein content and functional properties. However, knowledge of their nutrient composition and protein quality is necessary for the proper formulation of these ingredients in pet foods. The objective of this study was to determine the macronutrient composition and standardized amino acid digestibility and to describe the protein quality through the use of digestible indispensable amino acid scores (DIAAS-like) of five pulse ingredients. Black bean (BB) grits, garbanzo beans (GB), green lentils (GL), navy bean (NB) powder, and yellow peas (YP) were analyzed for dry matter (DM), ash and organic matter (OM), crude protein (CP), gross energy (GE), acid hydrolyzed fat (AHF), and total dietary fiber (TDF) to determine the macronutrient composition. Precision-fed rooster assays were conducted using cecectomized roosters to calculate standardized amino acid digestibility and true metabolizable energy corrected for nitrogen (TMEn). The essential amino acids, with the exception of methionine, were highly digestible with digestibility values of 80% to 90% (dry matter basis) for all selected pulse ingredients. BB grits had the lowest (P &lt; 0.05) digestibility of arginine (86.5%) and histidine (80.6%) in contrast to GB (94.9% and 89.9%, respectively). The TMEn of GB was highest (P &lt; 0.05) at 3.56 kcal/g compared with the other pulses. The DIAAS-like values for adult dogs were consistently the lowest for methionine for all pulses, making it the first-limiting amino acid in these ingredients. The DIAAS-like values for adult cats showed GL had lowest (P &lt; 0.05) score in tryptophan compared with other pulses when using both AAFCO values and NRC recommended allowances as reference proteins. Methionine was the first-limiting amino acid for YP and tryptophan for GL. Based on macronutrient composition, protein quality, and amino acid digestibility, it can be concluded that pulse ingredients have the required nutritional characteristics to be viable protein sources in canine and feline foods. However, the use of complementary protein sources is recommended to counterbalance any potential limiting amino acids in pulse ingredients.

Comparison of Interlaboratory Variation in Amino Acid Analysis and Rat Growth Assays for Evaluating Protein Quality

1985 ◽  
Vol 68 (1) ◽  
pp. 52-56 ◽  
Author(s):  
Ghulam Sarwar ◽  
Robert Blair ◽  
Mendel Friedman ◽  
Michael R Gumbmann ◽  
Ross L Hackler ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Analysis ◽  
Protein Quality ◽  
Essential Amino Acids ◽  
Quality Indices ◽  
Protein Ratio ◽  
Protein Sources ◽  
Rat Growth ◽  
Positive Correlations

Abstract Estimates of inter- and intralaboratory variation of protein efficiency ratio (PER), relative PER (RPER), net protein ratio (NPR), relative NPR (RNPR), and nitrogen utilization (NU) were compared with those of amino acid analysis in the same batches of 7 protein sources (ANRC casein, egg white solids, minced beef, soy assay protein, rapeseed protein concentrate, pea flour, and whole wheat flour). Interlaboratory variation (estimated as between-laboratories coefficients of variation, CV) of NPR and RNPR (up to 6.0%) was lower than that of PER (up to 20.2%) and RPER (up to 18.5%). The interlaboratory determination of NPR and RNPR was also more reproducible than that of most essential amino acids (CV up to 10.0%), especially tryptophan (CV up to 23.7%), cystine (CV up to 17.6%), and methionine (CV up to 16.1%). Intralaboratory variation (estimated as within-laboratories CV) of amino acid analysis (up to 4.7%), however, was comparable to that of protein quality indices in most protein sources (up to 6.0%). The significant (P &lt;0.01) positive correlations (r = 0.68-0.74) between amino acid scores and protein quality indices based on rat growth were further improved when amino acid scores were corrected for digestibility of protein (r = 0.73-0.78) or individual amino acids (r = 0.79- 0.82).

scholarly journals Efficiency of utilization of essential amino acids in growing rats at different levels of intake

1985 ◽  
Vol 54 (2) ◽  
pp. 499-508 ◽  
Author(s):  
J. Heger ◽  
Z. Frydrych
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Essential Amino Acid ◽  
Essential Amino Acids ◽  
N Balance ◽  
Growing Rats ◽  
Dietary Concentration ◽  
Limiting Amino Acid ◽  
Maintenance Requirements ◽  
Different Levels

1. Nitrogen balance was studied in growing male SPF-rats fed on diets in which each essential amino acid was varied from zero to about 120% of optimum requirement. From the balance results, optimum and maintenance requirements were estimated as well as the efficiency of utilization of amino acids for growth and growth + maintenance.2. N balance increased with increasing dietary level of the deficient amino acid; the response gradually diminished as the content of the amino acid approached optimum. At zero level of intake, negative N balance was found for all amino acids except histidine. The highest loss of body N was found in the sulphur-amino-acid-free diet and the lowest one in the lysine-free diet.3. Maximal utilization of essential amino acids for growth was found at dietary levels corresponding to 30–60% of optimum requirement and ranged from about 0.65 to 0.85 except for S amino acids and histidine. The utilization of S amino acids was about 0.55 while that of histidine exceeded 1.0. The utilization of amino acids for growth-tmaintenance was maximal at the lowest levels of intake and gradually decreased as the dietary concentration of the limiting amino acid increased. At dietary levels near optimum the utilization was about 06–07, except for S amino acids where the utilization was less than 0.5.

scholarly journals The effect of individual rye chromosomes on the amino acid content of wheat grains

1970 ◽  
Vol 15 (2) ◽  
pp. 209-219 ◽  
Author(s):  
Ralph Riley ◽  
J. A. D. Ewart
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Wheat Variety ◽  
Amino Acid Content ◽  
Essential Amino Acids ◽  
Rational Approach ◽  
Lysine Content ◽  
Wheat Grains ◽  
Group 5 ◽  
Chromosome I

SUMMARYAt the present time attempts are being made to improve the nutritional value of wheat by increasing the content of certain essential amino acids, particularly lysine, in the proteins of the grain. Rye grain has a considerably higher lysine content than that of wheat. Consequently in the present work studies were made of the amino acid contents of the grains of the wheat variety Holdfast, the rye variety King II, the Triticale derived from these parental varieties and the seven lines in which, in turn, each pair of chromosomes of King II are separately added to Holdfast.Rye chromosome I increased the lysine content of wheat by 8·7% and associated changes in the proportions of other amino acids suggest that this increase is meaningful. Rye chromosome I is in homoeologous group 5 and other reports have indicated a relationship between changed lysine content and another character determined by chromosomes of this group. Consequently there is a suggestion that group 5 chromosomes may be of particular significance in the determination of lysine content in wheat grains. Confirmation of this would lead to a more rational approach to breeding for higher lysine content.

Use of protein sources alternative to fish meal in diets with amino acids supplementation for the European eel (Anguilla anguilla)

1998 ◽  
Vol 66 (1) ◽  
pp. 285-292 ◽  
Author(s):  
M. García-Gallego ◽  
H. Akharbach ◽  
M. de la Higuera
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Essential Amino Acid ◽  
Fish Meal ◽  
Anguilla Anguilla ◽  
Essential Amino Acids ◽  
Protein Source ◽  
European Eel ◽  
Protein Sources ◽  
Meat Meal

AbstractThis experiment was conducted to test two different protein sources as alternatives to the commonly used fish meal (FM) in the diet of the European eel (Anguilla anguilla). Six experimental diets were tested in three replicated lots of European eels. All diets contained the same protein and energy content (ca, 300 g crude protein per kg dry matter and 18·5 MJ/kg, respectively) but differed in the nature of the protein source: FM was the only protein source in the control diet and was fully or partially (0–5: 0–5) replaced by meat meal (MM) or sunflower meal (SFM) in four other diets; a sixth diet included SFM as the only protein source but was supplemented with several essential amino acids. Food intake, fish growth and several indices of diet and protein utilization were measured. MM clearly was the poorest protein source while SFM could replace, at least 0·5 of the FM with no significant reduction in performance. In addition, the European eel was able to utilize the supplement of essential amino acids. The full-SFM diet was improved significantly when supplemented and results were not statistically different from the control FM-based diet. Overall, a good correlation was found between the results of each diet and the respective essential amino acid index, calculated using as reference the essential amino acid requirements previously defined for another eel species, Anguilla japonica. This index could be used as a reliable measure for an a priori evaluation of alternative protein sources to be included in commercial foods for eels.

scholarly journals Effect of fermentation methods on amino acids, fiber fractions and anti nutritional factors in different plant protein sources and essential amino acid index for Penaeus (Litopenaeus) vannamei

2017 ◽  
Vol 64 (2) ◽  
Author(s):  
R. Jannathulla ◽  
J. Syama Dayal ◽  
D. Vasanthakumar ◽  
K. Ambasankar ◽  
M. Muralidhar
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Essential Amino Acid ◽  
Plant Protein ◽  
Protein Sources ◽  
Shrimp Feed ◽  
Plant Protein Sources ◽  
Fiber Fractions ◽  
Fungal Fermentation ◽  
Acid Index

The incorporation of plant protein sources in shrimp feed is limited due to unbalanced amino acids and higher anti-nutrients. In the present study, soybean meal (SBM), groundnut oil cake (GNC), rapeseed meal (RSM), sunflower oil cake (SFC) and guar meal (GRM) were subjected to natural, bacterial, fungal and yeast fermentation methods. The essential amino acid contents were increased by 4-28% in SBM, 7-26% in GNC, 3-27% in RSM, 8-18% in SFC and 4-14% in GRM. The increase was better for lysine with fungal fermentation (2.31-4.01%). The improvement in other limiting amino acids viz., methionine and tryptophan also showed positive response to fermentation. The analytical results showed improved essential amino acid index (EAAI) in the fermented ingredients and the increase was better with RSM (0.82 to 0.92) using Aspergillus niger. Fiber fractions were reduced (p<0.05) in fungal and yeast treated samples but not due to natural or bacterial fermentation. The reduction of cellulose and hemicellulose was not only influenced by the inoculum but also on the ingredient used. The reduction of anti-nutrients (p<0.05) such as trypsin inhibitor, phytic acid, saponin, tannin, glucosinolate and guar gum were found to be lower in natural fermentation than other methods. The results indicated that fungal fermentation is more suitable for improving the nutritional quality of plant protein sources and this data will pave way for higher fishmeal replacement in shrimp feed formulations.

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