PROTEIN QUALITY EVALUATION OF COMMERCIAL AND LABORATORY HEAT-DAMAGED RAPESEED MEALS BY THE DYE-BINDING TECHNIQUE AND BY BIOLOGICAL ASSAY WITH CHICKS
The relationship between the protein quality index of commercial rapeseed meals (RSMs) and laboratory heat-damaged RSMs as predicted chemically by the dye-binding method and biologically by the total protein efficiency (TPE) method was investigated. Results indicated that commercial RSMs which bound Acid Orange 12 from 335 to 360 mg/g protein were not significantly different in protein quality as shown by two chick growth assays. The RSMs with DBCP of 327 mg Acid Orange 12/g protein or less were of lower protein quality as shown by chick growth assay. The TPE values recorded from RSMs heated for various lengths of time ranked in the same order as the DBCP and were a function of heating time.