DETERMINATION OF THE METHIONINE REQUIREMENT OF GROWING PIGS USING SERUM FREE AMINO ACIDS

1972 ◽  
Vol 52 (1) ◽  
pp. 163-169 ◽  
Author(s):  
M. O. KEITH ◽  
D. A. CHRISTENSEN ◽  
B. D. OWEN
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Free Amino Acids ◽  
Free Amino ◽  
Latin Square ◽  
Growing Pigs ◽  
Performance Data ◽  
Serum Free ◽  
Methionine Concentration

Six littermate Yorkshire gilts averaging 18 kg body weight were fed a semipurified diet containing six graded levels of methionine during six 4-day feeding periods in a Latin square design. Serum methionine concentrations were determined at the end of each period. Plotting serum methionine concentration against dietary methionine intake showed the methionine requirement to be 0.46% of the diet; this estimate was substantiated by animal performance data.

scholarly journals A METHOD FOR THE DETERMINATION OF FREE AMINO ACIDS IN RAT ORGANS AND TISSUES

1950 ◽  
Vol 182 (1) ◽  
pp. 29-37
Author(s):  
P.E. Schurr ◽  
H.T. Thompson ◽  
L.M. Henderson ◽  
C.A. Elvehjem
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Rat Organs

The decrease of some serum free amino acids can predict breast cancer diagnosis and progression

2019 ◽  
Vol 79 (1-2) ◽  
pp. 17-24 ◽  
Author(s):  
Dan Tudor Eniu ◽  
Florina Romanciuc ◽  
Corina Moraru ◽  
Iulian Goidescu ◽  
Daniela Eniu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Amino Acids ◽  
Cancer Diagnosis ◽  
Free Amino Acids ◽  
Free Amino ◽  
Breast Cancer Diagnosis ◽  
Serum Free

scholarly journals Standardized ileal digestible amino acids and net energy contents in full fat and defatted black soldier fly larvae meals (Hermetia illucens) fed to growing pigs

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Michelina Crosbie ◽  
Cuilan Zhu ◽  
Anna K Shoveller ◽  
Lee-Anne Huber
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Latin Square ◽  
Growing Pigs ◽  
Net Energy ◽  
Initial Body Weight ◽  
Black Soldier Fly ◽  
Digestible Energy ◽  
Black Soldier Fly Larvae ◽  
Fly Larvae

Abstract Two experiments were conducted to determine standardized ileal digestibility (SID) of amino acids (AA; Exp. 1) and net energy (Exp. 2) in two black soldier fly larvae meal (BSFLM) samples [full fat (FF; 42.5% crude protein (CP), as-fed) and defatted (DF; 40.8% CP; as-fed)] for growing pigs. Two cornstarch-based diets were formulated with FF and DF BSFLM as the sole sources of AA. A nitrogen-free diet was also used, and the corn starch:sucrose:oil ratio was kept constant among diets to calculate digestible energy (DE) by difference method. In each experiment, pigs were fed 2.8 × estimated maintenance energy requirement. In Exp. 1, eight ileal-cannulated barrows (25.1 ± 0.41 kg initial body weight) were used in a replicated 2 × 2 Latin square design (n = 8). In each period, pigs were adapted to diets for 5 d followed by 2 d of continuous ileal digesta collection for 8 h. The SID of AA were calculated using basal endogenous losses for pigs fed a nitrogen-free diet. In Exp. 2, eight barrows [23.4 ± 0.54 kg initial body weight (BW)] were used in a partially replicated Latin square design (n = 8). In each period, pigs were adapted to diets for 7 d, followed by 5 d of total urine collection and fecal grab sampling. The SID of CP (80.6 ± 1.1%) and Lys (88.0 ± 1.4%) were not different between FF and DF BSFLM. The SID of Arg, Val, Ala, and Pro tended to be less, and the SID of Met tended to be greater for the FF versus the DF BSFLM (P = 0.034, 0.090, 0.053, 0.065, 0.074, respectively). Digestible energy (4,927 vs. 3,941 ± 75 kcal/kg), metabolizable energy (4,569 vs. 3,396 ± 102 kcal/kg), and predicted net energy (3,477 vs. 2,640 ± 30 kcal/kg, using equations from Noblet; 3,479 vs. 2,287 ± 28 kcal/kg, using equations from Blok, respectively) were greater for the FF versus the DF BSFLM (P < 0.05). The apparent total tract digestibility of neutral detergent fiber and acid detergent fiber were greater for the FF versus the DF BSFLM (P ≤ 0.05). Both FF and DF BSFLM had high SID for most AA; however, FF BSFLM was a better source of net energy for growing pigs. Therefore, both FF and DF BSFLM could be used as protein alternatives in growing pig diets.

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