RELATIONSHIP BETWEEN TRANSFERRIN TYPE AND FEEDLOT PERFORMANCE IN BEEF HEIFERS

1966 ◽  
Vol 46 (3) ◽  
pp. 177-180 ◽  
Author(s):  
M. Makarechian ◽  
W. E. Howell
Keyword(s):  
Least Squares ◽  
Significant Influence ◽  
Gel Electrophoresis ◽  
Carcass Characteristics ◽  
Starch Gel Electrophoresis ◽  
Beef Heifers ◽  
Gene Frequencies ◽  
Feedlot Performance ◽  
Breed Group ◽  
Starch Gel

Sera from 120 feedlot heifers were analyzed by starch gel electrophoresis to determine their transferrin types. Estimates of the possible effects of different transferrin types on gain in the feedlot and some carcass characteristics were obtained by least squares methods. Analysis of variance showed that transferrin type did not have a significant influence on gain and carcass characteristics. Gene frequencies of the different transferrin alleles, derived from a breed group of 79 Hereford heifers, arc also reported.

Studies of the inheritance of bovine serum transferrins

1964 ◽  
Vol 6 (1) ◽  
pp. 107-117 ◽  
Author(s):  
G. A. E. Gall ◽  
R. T. Berg
Keyword(s):  
Gel Electrophoresis ◽  
Bovine Serum ◽  
Embryonic Mortality ◽  
Genetic Mechanism ◽  
Starch Gel Electrophoresis ◽  
Gene Frequencies ◽  
Starch Gel ◽  
Cattle Herds

SUMMARY1. The transferrins in two cattle herds were studied by means of starch gel electrophoresis. Three phenotypes were observed in a Hereford herd and six in a Hybrid herd comprised of Galloway, Aberdeen Angus and Charolais breeds.2. Gene frequencies were calculated for each herd. Great variation was found in the frequency of the transferrin alleles between breed and source within the Hybrid herd and between the Hybrid and Hereford herds. The frequencies of the transferrin alleles found in the cows in the Hereford herd were TfA = 0·467 and TfD = 0·533; those found in the cows in the Hybrid herd were TfA = 0·382, TfD = 0·506 and TfE = 0·112.3. Progeny data were in general agreement with the three-allele theory of inheritance, although the data suggested that offspring in the Hybrid herd carrying the TfE allele occurred at a lower frequency than expected.4. The possible relation of transferrin type to embryonic mortality was studied. The results were in general agreement with those of other workers, indicating a possible interaction between the genotypes of the foetus and dam.5. The characteristics of the transferrin types and the postulated three-allele genetic mechanism for the control of the observed variation are discussed.

The Red Cell Acid Phosphatase Polymorphism in Greek b-Thalassemia Patients

1975 ◽  
Vol 24 (3-4) ◽  
pp. 337-338
Author(s):  
A. Kalos ◽  
K. Melissinos ◽  
A. Archimandritis ◽  
G. Kourounis ◽  
B. Angelopoulos
Keyword(s):  
Acid Phosphatase ◽  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Red Cell ◽  
Gene Frequencies ◽  
Starch Gel ◽  
Acid Phosphatase Polymorphism ◽  
Cell Acid

Red cell acid phosphatase polymorphism was studied by starch gel electrophoresis in 70 b-thalassemia patients and in 310 healthy Greeks. Our results gave the following gene frequencies: b-thalassemia patients: pa 0.321, pb 0.643, pc 0.036; healthy Greeks: pa 0.302, pb 0.653, pc 0.045. No statistically significant differences were found between the two groups.

Serum Haptoglobin Phenotypes and Duodenal Ulcer

1977 ◽  
Vol 26 (1) ◽  
pp. 95-96
Author(s):  
A. Tsoukantas ◽  
A. Archimandrites ◽  
G. Theodoropoulos ◽  
K. Katsavochristos ◽  
K. Melissinos
Keyword(s):  
Duodenal Ulcer ◽  
Gel Electrophoresis ◽  
Healthy Subjects ◽  
Starch Gel Electrophoresis ◽  
Healthy Controls ◽  
Gene Frequencies ◽  
Serum Haptoglobin ◽  
Starch Gel

Serum haptoglobin phenotypes were studied in 100 Greek patients suffering from duodenal ulcer by vertical starch gel electrophoresis. A sample of 2026 healthy subjects served as control. No statistically significant differences were found in Hp phenotypes and gene frequencies between patients and healthy controls.

ASSOCIATION OF SERUM HEMOPEXIN, TRANSFERRIN, PREALBUMIN AND ALBUMIN1 TYPES WITH PRODUCTIVE TRAITS OF YORKSHIRE AND LACOMBE BREEDS OF PIGS

1969 ◽  
Vol 49 (2) ◽  
pp. 223-229 ◽  
Author(s):  
V. N. Tripathi ◽  
W. E. Howell
Keyword(s):  
Serum Protein ◽  
Gel Electrophoresis ◽  
Performance Test ◽  
Carcass Characteristics ◽  
Correlated Response ◽  
Starch Gel Electrophoresis ◽  
Starch Gel ◽  
The Difference ◽  
Productive Traits ◽  
Daily Gain

Sera from 354 Yorkshire and 97 Lacombe pigs on official Canadian Record of Performance test were analyzed by starch gel electrophoresis to determine their hemopexin, transferrin, prealbumin and albumin1 types. Estimates of the possible effects of different serum protein types on daily gain on test, age at slaughter adjusted to 70.37 kg carcass weight and seven other carcass characteristics were obtained by least squares methods. Significant associations were observed between each of the four serum protein loci and at least one of the nine productive traits in the Yorkshire breed. In the Lacombe breed only the prealbumin locus, which was associated with total backfat thickness, had any effect on any one of the nine productive traits. Since the results for the two breeds were not in agreement with each other and the magnitude of the difference between extremes was small, it was concluded that a correlated response in swine performance could not be expected from selection on the basis of serum protein polymorphism.

Fractionation of Plasminogen by Starch Gel Electrophoresis

1964 ◽  
Vol 12 (01) ◽  
pp. 126-136 ◽  
Author(s):  
Karl H. Slotta ◽  
J. D Gonzalez
Keyword(s):  
Gel Electrophoresis ◽  
Room Temperature ◽  
Broad Band ◽  
Caproic Acid ◽  
Starch Gel Electrophoresis ◽  
Full Activity ◽  
Veronal Buffer ◽  
Starch Gel ◽  
Alkaline Range

SummaryWhen urea or ε-amino caproic acid were used as solublizing agents for plasminogen in electrophoretic experiments, only one broad band of the proenzyme was obtained on acetate cellulose, in starch block, and in acrylamide gel. In starch gel electrophoresis, however, both forms of plasminogen – the native or euglobulin and Kline’s or Pseudoglobulin plasminogen – separated into six bands. These migrated toward the cathode at room temperature in borate or veronal buffer in the alkaline range and showed full activity in fibrinagar-streptokinase plates.

scholarly journals THE INHERITANCE OF ENZYME VARIANTS FOR TYROSINE AMINOTRANSFERASE, NADP-DEPENDENT MALATE DEHYDROGENASE, NADP-DEPENDENT ISOCITRATE DEHYDROGENASE, AND TETRAZOLIUM OXIDASE IN TETRAHYMENA PYRIFORMIS, SYNGEN 1

Genetics ◽  
1973 ◽  
Vol 74 (4) ◽  
pp. 595-603
Author(s):  
D Borden ◽  
E T Miller ◽  
D L Nanney ◽  
G S Whitt
Keyword(s):  
Detailed Analysis ◽  
Malate Dehydrogenase ◽  
Isocitrate Dehydrogenase ◽  
Gel Electrophoresis ◽  
Tetrahymena Pyriformis ◽  
Tyrosine Aminotransferase ◽  
Breeding Program ◽  
Starch Gel Electrophoresis ◽  
Enzyme Locus ◽  
Starch Gel

ABSTRACT The isozymic patterns of tyrosine aminotransferase, NADP malate dehydrogenase, NADP isocitrate dehydrogenase, and tetrazolium oxidase were examined by starch-gel electrophoresis in Tetrahymena pyriformis, syngen 1. The genetics of the alleles controlling these enzymes was studied through a breeding program. Each enzyme locus was shown to assort vegetatively, as do other loci in this organism. A detailed analysis of the assortment process for the tyrosine aminotransferase locus indicated that the rate of stabilization of heterozygotes into pure types was essentially identical to previously-reported rates for other loci.

STARCH GEL ELECTROPHORESIS OF MYOGEN FROM CHICKEN BREAST MUSCLE IN CONSTANT AND GRADIENT BUFFER SYSTEMS

1963 ◽  
Vol 41 (1) ◽  
pp. 369-387 ◽  
Author(s):  
J. M. Neelin
Keyword(s):  
Gel Electrophoresis ◽  
Protein Concentration ◽  
Breast Muscle ◽  
Chicken Breast ◽  
Starch Gel Electrophoresis ◽  
Two Dimensional ◽  
Sodium Cacodylate ◽  
Gradient Systems ◽  
Optimal Separation ◽  
Starch Gel

By varying conditions of starch gel electrophoresis, factors contributing to the resolution of myogen proteins from chicken breast muscle have been studied. Variables examined included composition of the myogen extractant, protein concentration, ionic strength of electrophoretic media, pH of gel media, plane and direction of electrophoresis, and the nature of cations and anions in gel media and bridge solutions. The significance of anions was more closely studied with constant buffer systems, and gradient systems in which bridge electrolyte differed from, and gradually altered, the gel medium. Optimal separation was obtained in gradient systems with 0.10 M sodium chloride bridge solutions, and gel media of sodium cacodylate, pH 6.9, μ 0.010, which resolved 12 cationic zones, and sodium veronal, pH 7.4, μ 0.010, which resolved 10 anionic zones. These buffers in two-dimensional sequence revealed a total of about 24 components in this myogen.

Morphological and biochemical systematics of chubs, Nocomis biguttatus and N. micropogon (Pisces: Cyprinidae), in southern Ontario

1981 ◽  
Vol 59 (5) ◽  
pp. 771-775 ◽  
Author(s):  
Moira M. Ferguson ◽  
David L. G. Noakes ◽  
Roy G. Danzmann
Keyword(s):  
Gel Electrophoresis ◽  
Function Analysis ◽  
Morphological Differentiation ◽  
Sexually Dimorphic ◽  
Starch Gel Electrophoresis ◽  
Southern Ontario ◽  
Electrophoretic Mobilities ◽  
Starch Gel ◽  
Respective Species ◽  
Probability Of Misclassification

Examination of 17 presumptive gene loci by starch-gel electrophoresis revealed differential mobilities only at acid phosphatase-1, alcohol dehydrogenase, esterase-1, and phosphoglucomutase between Nocomis biguttatus and N. micropogon. No intraspecific variation was observed for any loci. The genetic identity (I) and genetic distance (D) were 0.874 and 0.134, respectively. The correlation of electrophoretic mobilities and nuptial tubercle pattern in sexually dimorphic males supports the present taxonomic distinction of these species and provides a simple, unambiguous means of identifying any individuals.Stepwise discriminant function analysis of a series of mensural characters was used to compare fish identified as to species by electrophoresis. At best this correctly assigned fish to their respective species in 85.7% of cases, with a probability of misclassification of 0.1335.This study suggests these two are sibling species, based on a comparison of biochemical and morphological differentiation.

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