The collection of multiple saliva samples from pigs and the effect on adrenocortical activity

2013 ◽  
Vol 93 (3) ◽  
pp. 329-333 ◽  
Author(s):  
Nigel J. Cook ◽  
Stephanie M. Hayne ◽  
Fiona C. Rioja-Lang ◽  
Allan L. Schaefer ◽  
Harold W. Gonyou

Cook, N. J., Hayne, S. M., Rioja-Lang, F. C., Schaefer, A. L. and Gonyou, H. W. 2013. The collection of multiple saliva samples from pigs and the effect on adrenocortical activity. Can. J. Anim. Sci. 93: 329–333. The validity of collecting multiple saliva samples for the measurement of cortisol was tested in two sampling regimes in two weight classes of grower pigs (50 and 100 kg). The sampling regimes were a high-frequency, short-duration (HFSD) protocol involving collection of multiple samples within approximately 2 min of each other over a period 30 min. The second regime was a low-frequency, long-duration (LFLD) protocol in which samples were collected every 30 min for 3 h. Both sampling regimes were applied to individually housed pigs. The effect of repeated sampling of a focal pig on its cohorts in a group-housed pen was tested using the LFLD regime. There was no evidence of an effect of either of the sampling protocols on salivary cortisol concentrations in individually housed or group-housed pigs. There was some evidence that higher concentrations of salivary cortisol were associated with longer individual sampling durations in the HFSD regime for animals in the 50-kg weight class but not in the 100-kg weight class. The evidence from these experiments indicates that the collection of multiple saliva samples does not affect salivary cortisol concentrations in grower pigs, but that collection of individual samples in as short a time as possible would be prudent to avoid sampling effects in younger animals.

Author(s):  
Min Hu ◽  
Fan Du ◽  
Shi Liu

The purpose of this study was to investigate the effects of electroacupuncture at Zusanli acupoint on the enteric neuropathy in diabetic rats. Sprague–Dawley rats were divided into different groups depending on the total electroacupuncture span and frequency. The expression of nitric oxide synthase (nNOS), choline acetyltransferase (CHAT), protein gene product 9.5 (PGP9.5), and doublecortin was significantly decreased in the diabetic group compared with the control group. Long-term electroacupuncture at Zusanli with either high frequency or low frequency could increase the expression levels of nNOS, CHAT, PGP9.5, and doublecortin, and the increase was greater in the high-frequency group. But no obvious changes were seen in the short-term electroacupuncture groups. These results suggest that electroacupuncture at Zusanli can restore the deficiency of enteric neurons in diabetes partly but a comparative long duration of stimuli (6 weeks) is required. The increase of doublecortin may be involved in this positive process.


1996 ◽  
Vol 199 (4) ◽  
pp. 847-856 ◽  
Author(s):  
D Waters ◽  
G Jones

The noctuid moths Agrotis segetum and Noctua pronuba show peak auditory sensitivity between 15 and 25 kHz, and a maximum sensitivity of 35 dB SPL. A. segetum shows a temporal integration time of 69 ms. It is predicted that bats using high-frequency and short-duration calls will be acoustically less apparent to these moths. Short-duration frequency-modulated (FM) calls of Plecotus auritus are not significantly less acoustically apparent than those of other FM bats with slightly longer call durations, based on their combined frequency and temporal structure alone. Long-duration, high-frequency, constant-frequency (CF) calls of Rhinolophus hipposideros at 113 kHz are significantly less apparent than those of the FM bats tested. The predicted low call apparency of the 83 kHz CF calls of R. ferrumequinum appears to be counteracted by their long duration. It is proposed that two separate mechanisms are exploited by bats to reduce their call apparency, low intensity in FM bats and high frequency in CF bats. Within the FM bats tested, shorter-duration calls do not significantly reduce the apparency of the call at the peripheral level, though they may limit the amount of information available to the central nervous system.


1954 ◽  
Vol 32 (7) ◽  
pp. 450-467 ◽  
Author(s):  
D. W. R. McKinley

The reflecting properties of short-duration and long-duration echoes from meteor trails are examined. For short-duration echoes only, the observed relation between relative numbers and durations of meteor echoes is independent of wavelength, the time of day, or the presence of a strong shower. Integrated duration times are determined from back-scatter experiments on 9.22 m., 5.35 m., and 2.83 m., and are found to vary with the 3.5th power of the wavelength, for either short- or long-duration echoes. The integrated echo power depends on the 6th power of the wavelength. The effect of changing the equipment sensitivity is considered. The data from the back-scatter observations are used to predict the integrated duration times in the forward-scatter case, and in particular, it is shown that the results of the Cedar Rapids – Sterling very-high-frequency experiment may be explained by meteoric reflections.


1986 ◽  
Vol 250 (3) ◽  
pp. G336-G343 ◽  
Author(s):  
J. Crist ◽  
J. Gidda ◽  
R. K. Goyal

Longitudinal muscle strips from different sites along the opossum esophagus were stimulated transmurally so as to produce neurally mediated contractions. Low-frequency transmural stimulation produced contractions after termination of the stimulus ("off" contractions), whereas high-frequency stimuli produced contractions beginning during the stimulus and extending beyond termination of the stimulus (extended-duration contractions). The intrastimulus portion of the extended-duration contraction was partially antagonized by atropine or substance P desensitization, whereas the poststimulus portion of the contraction was selectively and fully antagonized by desensitization with substance P. A combination of atropine and substance P desensitization abolished the extended-duration contraction. The amplitude of contraction was greater in the proximal than in the distal strips, irrespective of the mode of stimulation. The poststimulus portion of the extended-duration contraction was significantly longer in muscle strips taken from more distal than proximal portions of the esophagus. This gradient in duration of contractions was abolished by substance P desensitization but was not affected by atropine. Exogenously applied substance P (10 microM) produced equally sustained long-duration contractions at all sites along the esophagus. These observations suggest that a) both acetylcholine- and substance P-containing nerves are responsible for the extended-duration contraction of longitudinal muscle, and b) transmural stimulation causes an aborally directed increase in the duration of contractions; this gradient of increasing duration of contraction appears to be due to a more prolonged neural release of substance P at more distal sites.


2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Sukonthar Ngampramuan ◽  
Puttichai Tungtong ◽  
Sujira Mukda ◽  
Apichat Jariyavilas ◽  
Chanin Sakulisariyaporn

Major depressive disorder (MDD) is associated with changes in autonomic nervous system (ANS) and cognitive impairment. Heart rate variability (HRV) and Pulse pressure (PP) parameters reflect influences of the sympathetic and parasympathetic nervous system. Cortisol exerts its greatest effect on the hippocampus, a brain area closely related to cognitive function. This study aims to examine the effect of HRV, PPG, salivary cortisol levels, and cognitive function in MDD patients by using noninvasive techniques. We have recruited MDD patients, diagnosed based on DSM-V-TR criteria compared with healthy control subjects. Their HRV and PP were measured by electrocardiogram (ECG) and photoplethysmography (PPG). Salivary cortisol levels were collected and measured on the same day. MDD patients exhibited elevated values of mean HR, standard deviation of HR (SDHR), low frequency (LF) power, low frequency/high frequency (LF/HF) ratio, mean PP, standard deviation of pulse pressure (SDPP), and salivary cortisol levels. Simultaneously, they displayed lower values of mean of R-R intervals (mean NN), standard deviation of R-R intervals (SDNN), high frequency (HF) power, and WCST scores. Results have shown that the ANS of MDD patients were dominated by the sympathetic activity and that they have cognitive deficits especially in the domain of executive functioning.


Author(s):  
G. Y. Fan ◽  
J. M. Cowley

It is well known that the structure information on the specimen is not always faithfully transferred through the electron microscope. Firstly, the spatial frequency spectrum is modulated by the transfer function (TF) at the focal plane. Secondly, the spectrum suffers high frequency cut-off by the aperture (or effectively damping terms such as chromatic aberration). While these do not have essential effect on imaging crystal periodicity as long as the low order Bragg spots are inside the aperture, although the contrast may be reversed, they may change the appearance of images of amorphous materials completely. Because the spectrum of amorphous materials is continuous, modulation of it emphasizes some components while weakening others. Especially the cut-off of high frequency components, which contribute to amorphous image just as strongly as low frequency components can have a fundamental effect. This can be illustrated through computer simulation. Imaging of a whitenoise object with an electron microscope without TF limitation gives Fig. 1a, which is obtained by Fourier transformation of a constant amplitude combined with random phases generated by computer.


Author(s):  
M. T. Postek ◽  
A. E. Vladar

Fully automated or semi-automated scanning electron microscopes (SEM) are now commonly used in semiconductor production and other forms of manufacturing. The industry requires that an automated instrument must be routinely capable of 5 nm resolution (or better) at 1.0 kV accelerating voltage for the measurement of nominal 0.25-0.35 micrometer semiconductor critical dimensions. Testing and proving that the instrument is performing at this level on a day-by-day basis is an industry need and concern which has been the object of a study at NIST and the fundamentals and results are discussed in this paper.In scanning electron microscopy, two of the most important instrument parameters are the size and shape of the primary electron beam and any image taken in a scanning electron microscope is the result of the sample and electron probe interaction. The low frequency changes in the video signal, collected from the sample, contains information about the larger features and the high frequency changes carry information of finer details. The sharper the image, the larger the number of high frequency components making up that image. Fast Fourier Transform (FFT) analysis of an SEM image can be employed to provide qualitiative and ultimately quantitative information regarding the SEM image quality.


1992 ◽  
Vol 1 (4) ◽  
pp. 52-55 ◽  
Author(s):  
Gail L. MacLean ◽  
Andrew Stuart ◽  
Robert Stenstrom

Differences in real ear sound pressure levels (SPLs) with three portable stereo system (PSS) earphones (supraaural [Sony Model MDR-44], semiaural [Sony Model MDR-A15L], and insert [Sony Model MDR-E225]) were investigated. Twelve adult men served as subjects. Frequency response, high frequency average (HFA) output, peak output, peak output frequency, and overall RMS output for each PSS earphone were obtained with a probe tube microphone system (Fonix 6500 Hearing Aid Test System). Results indicated a significant difference in mean RMS outputs with nonsignificant differences in mean HFA outputs, peak outputs, and peak output frequencies among PSS earphones. Differences in mean overall RMS outputs were attributed to differences in low-frequency effects that were observed among the frequency responses of the three PSS earphones. It is suggested that one cannot assume equivalent real ear SPLs, with equivalent inputs, among different styles of PSS earphones.


1971 ◽  
Vol 36 (4) ◽  
pp. 527-537 ◽  
Author(s):  
Norman P. Erber

Two types of special hearing aid have been developed recently to improve the reception of speech by profoundly deaf children. In a different way, each special system provides greater low-frequency acoustic stimulation to deaf ears than does a conventional hearing aid. One of the devices extends the low-frequency limit of amplification; the other shifts high-frequency energy to a lower frequency range. In general, previous evaluations of these special hearing aids have obtained inconsistent or inconclusive results. This paper reviews most of the published research on the use of special hearing aids by deaf children, summarizes several unpublished studies, and suggests a set of guidelines for future evaluations of special and conventional amplification systems.


2016 ◽  
Vol 17 (1) ◽  
pp. 66
Author(s):  
Maria Lina Silva Leite
Keyword(s):  

O objetivo deste estudo foi avaliar os efeitos do Método Pilates sobre a variabilidade da frequência cardíaca, na flexibilidade e nas variáveis antropométricas em indivíduos sedentários. O presente estudo contou com 14 voluntárias do sexo feminino, na faixa etária entre 40 e 55 anos, que realizaram 20 sessões de exercícios do Método Pilates, duas vezes por semana, com duração de 45 minutos cada sessão, dividida em três fases: repouso, exercício e recuperação. As variáveis estudadas foram: os dados antropométricos, flexibilidade avaliada utilizando o teste de sentar-e-alcançar com o Banco de Wells, e intervalos R-R usando um cardiotacômetro. O processamento dos sinais da frequência cardíaca foi efetuado em ambiente MatLab 6.1®, utilizando a TWC. Os dados coletados foram submetidos ao teste de normalidade de Shapiro Wilk e foi utilizado o teste de Wilcoxon e Anova One Way (α = 0,05). Nos resultados, observou-se que não houve diferenças significativas entre os valores antropométricos e de frequência cardíaca, porém houve aumento da flexibilidade com o treinamento. Comparando a primeira e a vigésima sessão com relação aos parâmetros low frequency (LF), high frequency (HF), e relação LF/HF, não houve diferença na fase de repouso e foram constatadas diferenças significativas de LF (p = 0,04) e HF (p = 0,04) na fase de exercício e diferença significativa de LF/HF (p = 0,05) na fase de recuperação. Comparando os parâmetros nos períodos de repouso, exercícios e recuperação durante a primeira sessão e durante a vigésima sessão, não houve diferença significativa nos parâmetros LF, HF e LF/HF. Pode-se concluir que, em relação à flexibilidade, foi observada uma melhora significativa, enquanto a análise da frequência cardíaca caracterizou a intensidade do exercício de 50% da capacidade funcional das voluntárias. Em relação aos parâmetros LF, HF e LF/HF foram observados um aumento da variabilidade da frequência cardíaca, provavelmente produto da atividade do Método Pilates. A Transformada Wavelet (TWC) mostrou-se um Método adequado para as análises da variabilidade da frequência cardíaca.Palavras-chave: frequência cardíaca, Transformada Wavelet, Pilates.


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