Effect of dry matter intake on visceral organ mass, cellularity, and the protein expression of ATP synthase, Na+/K+-ATPase, proliferating cell nuclear antigen and ubiquitin in feedlot steers

2009 ◽  
Vol 89 (2) ◽  
pp. 253-262 ◽  
Author(s):  
Y J Wang ◽  
M. Ko ◽  
S. Holligan ◽  
B W McBride ◽  
M Z Fan ◽  
...  
Keyword(s):  
Atp Synthase ◽  
Intestinal Mucosa ◽  
Proliferating Cell Nuclear Antigen ◽  
Dry Matter ◽  
Dry Matter Intake ◽  
Nuclear Antigen ◽  
Small Intestinal Mucosa ◽  
Organ Mass ◽  
Small Intestinal ◽  
Proliferating Cell

Twenty-four steers [467 ± 7.2 kg initial body weight (BW)] predominately of Angus breeding were used to determine the effect of dry matter intake (1.25, 1.50, 1.75, and 2.00% of BW) on visceral mass, cellularity, and the protein expression of ATP synthase, Na+/K+-ATPase, proliferating cell nuclear antigen (PCNA) and ubiquitin. There were linear increases (P ≤ 0.05) in weights of total viscera, total digestive tract, liver, kidney, heart, lung, spleen, rumen, and abomasum with increasing dry matter intake (DMI). Protein concentration decreased linearly (P < 0.05) in small intestinal mucosa as DMI increased. PCNA expression increased linearly (P < 0.01) in liver as DMI increased. PCNA expression was affected quadratically (P < 0.05) in pancreas and small intestinal mucosa with an increase when DMI increased from 1.25 to 1.75% of BW, and a decrease when DMI increased from 1.75 to 2% of BW. ATP synthase, Na+/K+-ATPase, and ubiquitin expression in pancreas and ubiquitin expression in small intestinal mucosa increased linearly (P < 0.05) as DMI increased. These results indicate that increasing DMI increases the mass of visceral organs and carcass and influences expression of proteins influencing energy utilization and efficiency in pancreas, small intestine, liver, and sternomandibularis muscle.Key words: Dry matter intake, visceral organ mass, cellular energy metabolism, steer

scholarly journals Effect of dietary corn silage inclusion on visceral organ mass, cellularity, and the protein expression of ATP synthase, Na+/K+-ATPase, proliferating cell nuclear antigen and ubiquitin in feedlot steers

2009 ◽  
Vol 89 (4) ◽  
pp. 503-512 ◽  
Author(s):  
Y J Wang ◽  
K M Wood ◽  
L Martin ◽  
S Holligan ◽  
N Kelly ◽  
...  
Keyword(s):  
Protein Expression ◽  
Atp Synthase ◽  
Proliferating Cell Nuclear Antigen ◽  
Corn Silage ◽  
Nuclear Antigen ◽  
Visceral Organ ◽  
Visceral Mass ◽  
Significant Treatment ◽  
Organ Mass ◽  
Proliferating Cell

Twenty-four steers (initial body weight = 535 ± 5.0 kg) predominately of Angus breeding were used to determine the effect of dietary corn silage inclusion [20, 40, 60, or 80% of dry matter (DM)] on visceral mass, cellularity, and the protein expression of ATP synthase, Na+/K+-ATPase, proliferating cell nuclear antigen (PCNA) and ubiquitin. Steers were fed at similar energy levels (2.1 × NEm requirement). There were no significant treatment effects on specific visceral organ weights. Hepatic Na+/K+-ATPase expression linearly increased (P = 0.01) and ruminal Na+/K+-ATPase expression linearly decreased (P = 0.01) with increasing corn silage inclusion. Hepatic PCNA expression was quadratically affected (P = 0.05) with a decrease when corn silage inclusion increased from 20 to 60%, and an increase when corn silage inclusion increased from 60 to 80%. Renal ATP synthase (P = 0.02) and ubiquitin expression (P = 0.01) were quadratically affected in a similar pattern with an increase when corn silage inclusion increased from 20 to 60%, and a decrease when corn silage inclusion increased from 60 to 80%. These results indicate that different dietary corn silage inclusions, at similar dietary energy intake, may alter rumen, liver, and kidney energy expenditure, at least in part, through changes in specific metabolism rather than mass. Key words: Corn silage inclusion, visceral organ mass, cellular energy metabolism, steer

Proliferating cells in the vertebrate small intestine: an immunohistocheniical study

1995 ◽  
Vol 53 ◽  
pp. 1034-1035
Author(s):  
Làszló G. Kömüves
Keyword(s):  
Small Intestine ◽  
Intestinal Mucosa ◽  
Ambystoma Mexicanum ◽  
Nuclear Antigen ◽  
Cell Renewal ◽  
Small Intestinal Mucosa ◽  
Proliferating Cells ◽  
Citrate Buffer ◽  
Small Intestinal ◽  
Proliferating Cell

In the small intestinal mucosa of healthy adult mammals proliferating cell are confined to the crypts of Lieberkiihn. Earlier radioautographic studies identified proliferative cells in the small intestine of several non-mammalian vertebrates. However, it is still not clear whether cell renewal is confined to proliferative compartment within the small intestinal mucosa in non-mammalian vertebrates. In the present study proliferative cells were identified using an immunological marker of cell proliferation, the proliferating cell nuclear antigen (PCNA) in the small intestine of several non-mammalian vertebrate species, including birds (zebrafinch, Poephila guttata), reptiles (green anole, Anolis carolinensis), amphibia (axolotl, Ambystoma mexicanum), and fishes (goldfish, Carassius auratus).Segments of the small intestine were fixed in 4% formaldehyde in 0.86 M phosphate buffer, pH=7.2 and embedded in paraffin. Deparaffinized and rehydrated sections were microwaved in citrate buffer. The immunohistochemical detection method used in this study based on the capillary action principle, as developed by Brigati.

Effect of dietary crude protein level on visceral organ mass, cellularity, and the protein expression of ATP synthase, Na+/K+-ATPase, proliferating cell nuclear antigen and ubiquitin in feedlot steers

2009 ◽  
Vol 89 (4) ◽  
pp. 493-501 ◽  
Author(s):  
Y J Wang ◽  
S Holligan ◽  
H Salim ◽  
M Z Fan ◽  
B W McBride ◽  
...  
Keyword(s):  
Small Intestine ◽  
Protein Expression ◽  
Atp Synthase ◽  
Proliferating Cell Nuclear Antigen ◽  
Crude Protein ◽  
Nuclear Antigen ◽  
Visceral Organ ◽  
Organ Mass ◽  
Dietary Crude Protein ◽  
Proliferating Cell

Twenty-four steers [initial body weight (BW) = 510 ± 4.9 kg] predominately of Angus breeding were used to determine the effect of dietary crude protein (CP) level [8.5, 10.7, 12.3 or 14.5%, dry matter (DM) basis; high-moisture-corn-based diets] on visceral mass, cellularity, and protein expression of ATP synthase, Na+/K+-ATPase, proliferating cell nuclear antigen (PCNA) and ubiquitin. Steers were on dietary treatment for 28 d. Kidney, liver, and reticulorumen weights (g) increased linearly (P < 0.05) with increased dietary CP. Lung weight (g; g kg-1 BW) linearly increased (P < 0.05) with increased CP. Ruminal and small intestinal DNA concentration, and the ratios of RNA:DNA and protein:DNA in small intestine were affected quadratically by increased dietary CP (P < 0.05). Hepatic ATP synthase expression was affected quadratically with an increase when dietary CP increased from 8.5 to 10.7%, and a decrease when dietary CP increased from 10.7 to 14.5% (P < 0.05). Renal ATP synthase expression decreased linearly (P < 0.05) and small intestine mucosal Na+/K+-ATPase expression increased linearly (P = 0.05) with increased CP. These results indicate that increasing dietary CP increases liver, kidney, lung, and rumen masses, and differentially influences expression of proteins influencing energy utilization and efficiency in liver, kidney, and small intestine.Key words: Dietary crude protein, visceral organ mass, cellular energy metabolism, steer

scholarly journals A bone marrow stromal-derived growth factor, interleukin-11, stimulates recovery of small intestinal mucosal cells after cytoablative therapy

Blood ◽  
1994 ◽  
Vol 83 (1) ◽  
pp. 33-37 ◽  
Author(s):  
XX Du ◽  
CM Doerschuk ◽  
A Orazi ◽  
DA Williams
Keyword(s):  
Growth Factor ◽  
Intestinal Mucosa ◽  
Nuclear Antigen ◽  
Small Intestinal Mucosa ◽  
Nuclear Antigens ◽  
Mucosal Cells ◽  
Small Intestinal ◽  
Proliferating Cell ◽  
Interleukin 11 ◽  
Intestinal Mucosal Cells

The proliferation of epithelial cells lining the small intestinal mucosa may be regulated by microenvironmental signals leading to differentiation of precursor cells in the small intestinal crypts. Proliferation of hematopoietic cells within the hematopoietic microenvironment is known to be regulated by a growing number of glycoprotein growth factors in a hierarchial fashion. We studied the effects of administration of the microenvironment-derived hematopoietic growth factor interleukin-11 (IL-11) on mice given combination radiation/chemotherapy. Treatment of such mice with IL-11 led to significantly increased survival and evidence of rapid recovery of the small intestinal mucosa, which is severely damaged by these cytoxic agents. This recovery was associated with an increase in the mitotic index of crypt cells and an increased frequency of staining of these cells with a monoclonal antibody to proliferating cell nuclear antigen, a member of the cyclin family of nuclear antigens.

scholarly journals A bone marrow stromal-derived growth factor, interleukin-11, stimulates recovery of small intestinal mucosal cells after cytoablative therapy

Blood ◽  
1994 ◽  
Vol 83 (1) ◽  
pp. 33-37 ◽  
Author(s):  
XX Du ◽  
CM Doerschuk ◽  
A Orazi ◽  
DA Williams
Keyword(s):  
Growth Factor ◽  
Intestinal Mucosa ◽  
Nuclear Antigen ◽  
Small Intestinal Mucosa ◽  
Nuclear Antigens ◽  
Mucosal Cells ◽  
Small Intestinal ◽  
Proliferating Cell ◽  
Interleukin 11 ◽  
Intestinal Mucosal Cells

Abstract The proliferation of epithelial cells lining the small intestinal mucosa may be regulated by microenvironmental signals leading to differentiation of precursor cells in the small intestinal crypts. Proliferation of hematopoietic cells within the hematopoietic microenvironment is known to be regulated by a growing number of glycoprotein growth factors in a hierarchial fashion. We studied the effects of administration of the microenvironment-derived hematopoietic growth factor interleukin-11 (IL-11) on mice given combination radiation/chemotherapy. Treatment of such mice with IL-11 led to significantly increased survival and evidence of rapid recovery of the small intestinal mucosa, which is severely damaged by these cytoxic agents. This recovery was associated with an increase in the mitotic index of crypt cells and an increased frequency of staining of these cells with a monoclonal antibody to proliferating cell nuclear antigen, a member of the cyclin family of nuclear antigens.

Sign in / Sign up

Export Citation Format

Share Document