Effect of supplementing phytase to corn- or wheat-based gestation and lactation diets on nutrient digestibility and sow and litter performance

2006 ◽  
Vol 86 (4) ◽  
pp. 501-510 ◽  
Author(s):  
C. M. Nyachoti ◽  
J. S. Sands ◽  
M. L. Connor ◽  
O. Adeola
Keyword(s):  
Escherichia Coli ◽  
Key Words ◽  
Positive Control ◽  
Negative Control ◽  
Nutrient Digestibility ◽  
Phytate Phosphorus ◽  
Period 2

Effect of an Escherichia coli derived, 6-phytase on nutrient digestibility and sow and litter performance were investigated. Six (exp. 1) or eight (exp. 2) sows per diet were randomly assigned to a positive control (PC), a negative control (NC) with reduced non-phytate phosphorus (P), NC + 500 FTU kg-1, or NC + 1000 FTU kg-1 diet from day 60 of gestation to day 22 postpartum. Diets in exps. 1 and 2 were based on corn and wheat, respectively. Energy, N, calcium (Ca), and P digestibilities were determined between days 73 to 77 (Period 1), 104 to 106 (Period 2) of pregnancy, and days 16 to 20 postpartum. Sow and litter performance were monitored. During gestation, phytase at 500 FUT kg-1 did not affect nutrient digestibility in exp. 1 but improved (P < 0.05) DE and digestibilities of DM, energy, N, and P in exp. 2. Increasing phytase to 1000 FTU kg-1 reduced (P < 0.05) digestibilities of energy and N (exp. 1). In both experiments, digestibilities of N, Ca, and P were higher (P < 0.05) in period 2 than period 1. During lactation, supplementing the NC diet with 500 FTU kg-1 only improved (P < 0.05) DE and Ca and P digestibility in exp. 1 and DE and digestible P in exp. 2. Compared with NC, P digestibility and digestible P were higher (P < 0.05) in the NC + 1000 FTU kg-1 diet in both experiments. Sow and piglet performance was unaffected by diet in both experiments. In conclusion, P digestibility was greater between days 104 and 106 of gestation than between days 73 and 77 of gestation and phytase improved P digestibility during lactation but this effect was not consistent during gestation in both experiments. Key words: Digestibility, phytase, phosphorus, sows

scholarly journals Effect of supplementation with Escherichia coli phytase for broilers on performance, nutrient digestibility, minerals in the tibia and diet cost

2019 ◽  
Vol 40 (2) ◽  
pp. 767
Author(s):  
Gabriel Villela Dessimoni ◽  
Nilva Kazue Sakomura ◽  
Daniella Carolina Zanardo Donato ◽  
Fábio Goldflus ◽  
Nayara Tavares Ferreira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Broiler Chickens ◽  
Crude Protein ◽  
Control Diet ◽  
Positive Control ◽  
Negative Control ◽  
Nutrient Digestibility ◽  
Conversion Ratio ◽  
Feed Conversion ◽  
Diet Cost

Although phytase has been researched, new enzymes have been produced, leading to different animal responses. In this scenario, the present study proposes to evaluate the inclusion of a bacterial phytase produced by Escherichia coli in broiler diets based on corn and soybean meal, with or without nutrient reductions, on the performance, nutrient digestibility, phosphorus bioavailability, and bone minerals of those animals and on the economic viability of this practice. A total of 896 male broiler chickens were distributed into 32 experimental units, each housing 28 broilers. The experiment was set up as a completely randomized design with four treatments (Positive Control (PC)- diet meeting the nutritional requirements of the broiler chickens; Negative Control (NC) with reductions of 100kcal/kg of ME, 0.14% avP and 0.11% tCa; NC + phytase (500 FTU/kg); PC + phytase (500FTU/kg)) and 8 replicates. Phytase increased (p < 0.05) feed intake and body weight gain and improved feed conversion ratio in starter (1 to 21 days) and total (1 to 42 days) phases, respectively, compared with Negative Control diet without supplementation. The Negative Control + phytase diet also led to a feed intake similar to Positive Control in the starter and total phases. The inclusion of phytase without nutrient reductions improved (p < 0.05) feed conversion ratio in the starter phase, compared to Positive Control diet. There was an increase (p < 0.05) in the digestibility of dry matter, crude protein and phosphorus and in apparent digestible energy in the Negative Control + phytase diet compared to the Negative Control diet. Phytase supplementation increased (p < 0.05) the digestibility of crude protein, calcium and phosphorus, and apparent digestible energy compared to Positive Control diet. Ash, phosphorus, and calcium contents were higher in the Negative Control + phytase diet compared with those observed in the Negative Control diet without enzyme (p < 0.05). The Positive Control + phytase diet provided higher ash contents (p < 0.05), but calcium and phosphorus deposition was similar to those obtained with Positive Control diet. Phytase inclusion allows for a reduction in the diet cost per ton of produced feed. In conclusion, dietary supplementation with bacterial phytase produced from Escherichia coli for broiler chickens is recommended, as it provided increases in production performance, nutrient digestibility, and energy metabolizability and a reduction in the diet cost.

Thermo-stable Escherichia coli phytase improves growth performance and nutrient utilization in broilers fed mash and pelleted corn-soybean-meal-based diets

2010 ◽  
Vol 90 (4) ◽  
pp. 529-536 ◽  
Author(s):  
T.A. Woyengo ◽  
A. Emiola ◽  
A. Owusu-Asiedu ◽  
W. Guenter ◽  
P.H. Simmins ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Body Weight ◽  
Weight Gain ◽  
Body Weight Gain ◽  
Nutrient Utilization ◽  
Positive Control ◽  
Negative Control ◽  
Coating Technology ◽  
Phytate Phosphorus ◽  
Tibia Ash

The bio-efficacy of Escherichia coli-derived phytase produced by thermo-protective coating technology (coated phytase) was evaluated in mash or pelleted broiler diets. Three hundred and twenty-four broilers were divided into 54 groups of six birds and fed nine corn-based diets (six replicates per diet) from 1 to 22 d of age. The nine diets were a positive control (PC) (calcium, 0.9% and non-phytate phosphorus, 0.45%) fed as mash plus 2 × 4 factorial of a negative control (calcium, 0.78% and non-phytate phosphorus, 0.26%) fed as mash (NC-M) or pellet (NC-P) and with coated phytase at 0, 500, 600 or 700 FTU kg-1. The diets were pelleted at 80°C, and pressure load of 40 psi. Body weight gain (BWG) for the PC diet was higher (P < 0.001) than that for the NC-M or NC-P diet. The BWG and tibia ash were linearly increased (P < 0.001) by increased level of the phytase in NC-M or NC-P diet. The BWG for NC-M or NC-P diet reached that of the PC diet when phytase was supplemented at 600 or 700 FTU kg-1. In conclusion, the coated phytase improved nutrient utilization in broilers, and its bio-efficacy was unaffected by the pelleting process.

scholarly journals Evaluation of antipyretic activity of hydroalcoholic extract of Corchorus depressus Linn. in Escherichia coli–induced pyretic rabbits

2018 ◽  
Vol 16 ◽  
pp. 205873921879295
Author(s):  
Saeed Ahmad ◽  
Muhammad Akram ◽  
Syed Muhammad Ali Shah ◽  
Sabira Sultana
Keyword(s):  
Escherichia Coli ◽  
Positive Control ◽  
Negative Control ◽  
The Body ◽  
Hydroalcoholic Extract ◽  
Antipyretic Effect ◽  
E Coli ◽  
Antipyretic Activity ◽  
Experimental Treatments

This study was conducted to investigate the antipyretic effect of the hydroalcoholic extract of Corchorus depressus Linn. against Escherichia coli ( E. coli)-induced pyrexia in rabbits. Hydroalcohalic extracts of C. depressus were given orally at 25, 50, and 100 mg/kg for antipyretic affect in E. coli-induced fever in rabbits. The animals were divided into five groups of five each. Among these five groups, three received various doses of experimental treatments, whereas the fourth one served as positive control and received paracetamol. The fifth group of animals served as negative control and received no treatment. The body temperature of the rabbits was measured rectally over a period of 5 h. C. depressus exhibited better effects at dose rate of 25, 50, and 100 mg/kg. The hydroalcoholic extract of C. depressus has significant antipyretic effect. These results lend support to the popular use of C. depressus in traditional medicine as a remedy for pyrexia and suggest that the characterization of the principles for such activity deserves further investigation.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK PERASAN DAUN MENGKUDU (Morinda citrifolia L.) TERHADAP Escherichia coli SECARA IN VITRO

2020 ◽  
Vol 2 (1) ◽  
pp. 26-34
Author(s):  
Margareta Retno Priamsari ◽  
Agastia Cicilia Wibowo
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Drying Shrinkage ◽  
Positive Control ◽  
Negative Control ◽  
Morinda Citrifolia ◽  
Diffusion Method ◽  
Juice Extraction ◽  
Water Inhibition

Noni juice can inhibit the growth of Escherichia coli bacteria. Noni juice extraction needs concentration to extract so that the preparation is more stable in the storage process. The purpose of this study was to determine the antibacterial activity and the amount of the minimum inhibitory concentration of noni juice extract from E. coli bacteria in vitro. This type of experimental research with a completely randomized one-way design. The extract was obtained by concentrating the Noni leaf extract. Extract quality control parameters include organoleptic, yield, drying shrinkage, and qualitative tests of flavonoid and anthraquinone compounds. Antibacterial activity test using the disc diffusion method with an extract concentration of 1.56%; 3.12%; 6.25%; 12.5%; and 25% with 3 replications. Positive control of amoxicillin and negative control of distilled water. Inhibition is known from the zone formed around the paper disc. The data obtained were statistically analyzed using Kruskall Wallis followed by Mann Whitney with a 95% confidence level. The results showed that the variation in the concentration of the noni juice extract had a significant effect (p <0.05). The biggest inhibitory zone was seen at 25% concentration of 10.16 mm and included in the strong category. The minimum inhibitory power was produced at a concentration of 3.12% at 2.50 mm with a weak treatment category.

20 Effect of Adding Bioactive Peptide in Combination of Pharmaceutical Zinc Oxide or Organic Acids on Growth Performance, Hematology Profile, and Nutrient Digestibility in Nursery Pigs

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 22-23
Author(s):  
Richard A Mudarra ◽  
Tsung Cheng Cheng Tsai ◽  
Kristopher Bottoms ◽  
Thomas S Shieh ◽  
Casey Bradly ◽  
...  
Keyword(s):  
Growth Performance ◽  
Phase 1 ◽  
Random Effect ◽  
Positive Control ◽  
Negative Control ◽  
Nutrient Digestibility ◽  
Bioactive Peptide ◽  
Nursery Pigs ◽  
Cell Counts ◽  
High Zinc

Abstract To evaluate the effect of bioactive peptide (P) in combination with high level of zinc (HZ) or acidifiers on growth performance, complete blood cell counts (CBC) and nutrient digestibility in nursery pigs, a total of 288 weaned pigs (PIC1050xDNA600) were stratified by initial BW within gender and allotted to 1of 7 treatments. Treatments for phase 1&2 were: 1) nutrient adequate positive control with HZ (PC), 2) nutrient deficient negative control with HZ (NC, -0.13% SID Lysine by reducing fish meal), 3) NC+0.25% peptide (0.25PZ), 4) NC+0.5% peptide (0.5PZ), 5) NC+0.25% peptide with standard zinc (0.25P), 6) NC+0.5% peptide with standard zinc (0.5P), 7) as 5 + 0.1% sodium butyrate and 0.5% benzoic acid (PSB). All pigs were fed a common low Zn diet (197 ppm) during phase 3. The whole blood was obtained from a close-to-average pen-BW pig repeatedly at weaning, and at the end of phase 2 and 3 to determine CBC. Titanium dioxide was used as an indigestible marker to determine nutrient digestibility. Data were analyzed using the Mixed procedures of SAS as a RCBD with treatment as fixed effect, and BW block as random effect. In overall phase 1&2, pigs fed PSB had similar ADG and BW when compared to pigs fed 0.25PZ and both were greater than NC pigs (Table 1). With the same inclusion rate of peptide, pigs fed a high zinc diet had greater BW and ADG than pigs fed a standard zinc diet. PSB pigs had the greatest G:F ratio and nitrogen digestibility among treatments. Increasing peptide in high zinc diets gradually decreased Neutrophil-to-lymphocyte ratio. This study indicates that the improvement in growth performance from pigs fed peptide is pharmaceutical zinc dependent and acidifiers can be an alternative to replace ZnO without affecting growth performance.

Protective Effect of Curcumin on Carbapenem-Resistant Escherichia coli–Induced Lung Injury in Rats

2016 ◽  
Vol 101 (7-8) ◽  
pp. 304-312 ◽  
Author(s):  
Cagla Bali ◽  
Nejat Altintas ◽  
Ozlem Ozmete ◽  
Ibrahim Gelincik ◽  
Hakan Yabanoglu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lung Injury ◽  
Intraperitoneal Injection ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
E Coli ◽  
Carbapenem Resistant

Curcumin has remarkable anti-inflammatory and antioxidant properties. The aim of this study was to investigate the protective effects of curcumin on a rat model of carbapenem-resistant Escherichia coli–induced acute lung injury (ALI). Thirty-two rats were randomly allocated to 4 groups to induce an ALI: negative control group (rats not infected with E coli with no antibiotic treatment), positive control group (rats infected with E coli with no antibiotic treatment), imipenem group (rats infected with E coli that received intraperitoneal injection of imipenem), and the imipenem+curcumin group (rats infected with E coli that received intraperitoneal injection of imipenem and were fed on curcumin).The rats were killed, and lung tissues samples were harvested for biochemical analyses and histopathologic examination. Total antioxidant status (TAS), total oxidant status (TOS), tumor necrosis factor α (TNFα), and interleukin-6 (IL6) were measured. TOS increased in the positive control group (P &lt; 0.001) and decreased in the imipenem and imipenem+curcumin groups (P &lt; 0.001 and P &lt; 0.001, respectively). TAS decreased in the positive control group (P = 0.005). Imipenem treatment did not increase TAS, but the imipenem+curcumin group increased TAS (P = 0.014). TNFα and IL6 increased in the positive control group compared with the negative control group (P &lt; 0.001 and P = 0.010, respectively). Imipenem decreased TNFα (P &lt; 0.001), but did not decrease IL6 (P = 0.418). Imipenem+curcumin decreased TNFα (P &lt; 0.001); this decrease was more pronounced compared with the imipenem group (P = 0.008). IL6 decreased in the curcumin group compared with the positive control group (P = 0.011). Curcumin combined with imipenem can be an alternative therapeutic agent to overcome the resistance of E coli strains.

scholarly journals Nutrient digestibility of multi-enzyme supplemented low-energy and AA diets for grower pigs1

2019 ◽  
Vol 97 (7) ◽  
pp. 2979-2988
Author(s):  
Jung Wook Lee ◽  
Robert Patterson ◽  
Anna Rogiewicz ◽  
Tofuko A Woyengo
Keyword(s):  
Latin Square ◽  
Nutrient Utilization ◽  
Positive Control ◽  
Negative Control ◽  
Linear Increase ◽  
Nutrient Digestibility ◽  
Low Energy ◽  
Partial Replacement ◽  
Complete Replacement ◽  
Corn Distillers

Abstract A study was conducted to determine effects of supplementing multi-enzyme on apparent ileal digestibility (AID) of energy and AA; and apparent total tract digestibility (ATTD) of energy for pigs fed low-energy and AA diets. Eight ileal-cannulated barrows (initial BW: 38.7 ± 2.75 kg) were fed four diets in a replicated 4 × 4 Latin square design to give 8 replicates per diet. Diets were positive control (PC) diet, negative control (NC) diet without or with multi-enzyme at 0.5 or 1.0 g/kg. The PC diet was formulated to meet or exceed NRC (2012) nutrient recommendations for grower pigs (25 to 50 kg), except for Ca and digestible P, which were lower than NRC (2012) recommendations by 0.13 and 0.17 percentage points, respectively, due to phytase supplementation at 1,000 FTU/kg. The NC diet was formulated to be lower in NE by 75 kcal/kg and standardized ileal digestible AA content by a mean of 3%. These reductions were achieved by partial replacement of corn and soybean meal (SBM) and complete replacement of soybean oil and monocalcium phosphate in PC diet with 25% corn distillers dried grains with solubles (DDGS) and 3.6% soybean hulls. Multi-enzyme at 1.0 g/kg supplied 1,900 U of xylanase, 300 U of β-glucanase, 1,300 U of cellulase, 11,500 U of amylase, 120 U of mannanase, 850 U of pectinase, 6,000 U of protease, and 700 U of invertase per kilogram of diet. The AID of GE, N, most AA, most component sugars of nonstarch polysaccharides (NSP) and P; ATTD of GE for PC diet was greater (P < 0.05) than those for NC diets. An increase in dietary level of multi-enzyme from 0 to 1.0 g/kg resulted in a linear increase (P < 0.05) in AID of Ile by 4.3%, and tended to linearly increase (P < 0.10) AID of Leu, Met, Phe, and Val by a mean of 3.4%. Increasing dietary multi-enzyme from 0 to 1.0 g/kg linearly increased (P < 0.05) AID of total NSP and P by 53.7% and 19.2%, respectively; ATTD of GE by 8.4% and DE and NE values by 8.8% and 8.2%, respectively; tended to linearly increase (P < 0.10) AID of GE by 8.1%. The NE values for NC diet with multi-enzyme at 1.0 g/kg tended to be greater (P < 0.10) than that for PC diet (2,337 vs. 2,222 kcal/kg of DM). In conclusion, multi-enzyme supplementation improved energy and nutrient digestibilities of a corn–SBM–corn DDGS-based diet, implying that the multi-enzyme fed in the current study can be used to enhance energy and nutrient utilization of low-energy AA diets for grower pigs.

scholarly journals Effects of alphamune G on the performance, serum and haematological parameters of Escherichia coli challenged turkey poults

2020 ◽  
Vol 40 (2) ◽  
pp. 112-121
Author(s):  
S. A. Bolu ◽  
M. T. Adelakun
Keyword(s):  
Escherichia Coli ◽  
Poor Performance ◽  
Positive Control ◽  
Negative Control ◽  
E Coli ◽  
Turkey Poults ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Improved Performance ◽  
Haematological Indices

A study was conducted to determine the response of Turkey poults to graded levels of Alphamune G (0.00+, 0.03, 0.04, 0.05, 0.06 and 0.00 %-) when challenged with Escherichia coli orally for 7 days. The graded levels were the treatments viz 0.00%+ (positive control), Alphamune G at 0.03, 0.04, 0.05, 0.06% and 0.00%- (negative control; infected without Alphamune G supplementation). Each treatment was allotted 3 replicates of 6 poults. The experiment which was conducted for 56 days employed a completely randomized design. E. coli was isolated from the intestinal digesta of a colisepticaemic chicken. 108 turkey poults were used in this study. Poults were infected with E.coli for 7 days through the drinking water and given the treatment. The performance parameters of Alphamune G supplementation were significantly affected. The cumulative weight, Feed intake and weight gain were highest for turkey poults fed 0.06% Alphamune G supplementation. These values were also directly proportional to the supplementation levels of Alphamune G. The birds given the negative treatment (0.00 %-) had relatively poor performance compared to the other treatments. The specific enzymes studied were significantly affected (p<0.05) by the treatments. ALT and AST were significantly highest for turkey poults fed the negative control. Enzyme values became optimum at 0.05% Alphamune G supplementation. At 0.06% of Alphamune G supplementation, cellular mitigations of the effects of E. coli was measurable. Urea and creatinine were not significantly (p>0.05) influenced by the treatments. Haematological indices such as WBC and specific differential counts (lymphocytes and neutrophils) were affected significantly (P<0.05) by supplemental levels of Alphamune G The Inclusion of Alphamune G at 0.06% in the diets improved performance of turkey poults when challenged with Escherichia coli.

scholarly journals PEMBERIAN RANSUM KOMBINASI HERBAL DENGAN MINERAL ZINK TERHADAP PERFORMANS AYAM BROILER YANG DIINFEKSI Escherichia coli

ZOOTEC ◽  
2013 ◽  
Vol 33 (1) ◽  
pp. 35
Author(s):  
M. N. Regar ◽  
R. Mutia ◽  
S. D. Widhyari ◽  
Y. H. S. Kowel
Keyword(s):  
Escherichia Coli ◽  
Sweet Potato ◽  
Basal Diet ◽  
Positive Control ◽  
Negative Control ◽  
E Coli ◽  
Garlic Powder ◽  
Ipomea Batatas ◽  
Sweet Potato Leaves ◽  
Better Than

ABSTRAKPenelitian ini dilakukan untuk menguji pengaruh pemberian kombinasi herbal dengan mineral zink dalam ransum broiler yang diinfeksi Escherichia coli (E. coli). Dua ratus ekor d.o.c (day old chick) dibagi secara acak ke dalam lima perlakuan, masing-masing perlakuan diulang empat kali sehingga terdapat 20 unit percobaan, setiap unit percobaan terdiri dari 10 ekor d.o.c. Anak ayam percobaan dipelihara selama 35 hari. Ransum perlakuan terdiri dari R1 = Pakan basal/ ayamsehat (kontrolnegatif); R2 = Pakan basal/ ayamdiinfeksiE.coli (kontrolpositif); R3= Pakan basal + serbukkunyit 1.5% + ZnO 180 ppm/ ayamdiinfeksiE.coli; R4 = Pakan basal + serbukbawangputih 2.5% + ZnO 180 ppm/ ayamdiinfeksiE.coli; R5 = Pakan basal + antibiotik/ ayamdiinfeksiE.coli.Penelitian menggunakan rancangan acak lengkap. Peubah yang diamati konsumsi ransum, pertambahan bobot badan, dan konversi ransum. Hasil dari penelitian ini menunjukkan bahwa pemberian kombinasi kunyit 1.5% dengan ZnO 180 ppm dan kombinasi bawang putih 2.5% dengan zink 180 ppm dalam ransum mampu memperlihatkan performa yang lebih baik.Kata Kunci:Daun ubi jalar, Pertambahan berat badan, Konsumsi ransum, Konversi ransum, Ayambroiler.ABSTRACTTHE EFFECT OF SUPPLEMENTA-TION SWEET POTATO LEAVES MEAL (Ipomea batatas) ON BROILER PERFORMANCE.This experiment was conducted to study the combination of herbal with zink in poultry diet on the performance of Escherichia coli – challenged broiler. Two hundred d.o.c unsexed were devided into five treatments and four replications, with ten chicks in each replicates. The treatments were R1 (basal diet as a negative control/ healhty chickens), R2 (basal diet as a positive control/ Escherichia coli challenged), R3 (basal diet +1.5% turmeric powder + ZnO 180 ppm/ Escherichia coli challenged), R4 (basal diet + 2.5% garlic powder + ZnO 180 ppm/ Escherichia coli challenged), and R5 (basal diet + antibiotic/ Escherichia coli challenged). Data were collected during 35 days, diet and water were offered ad libitum. The results of this research indicated that chickens fed basal diet + 1.5% turmeric powder + ZnO 180 ppm/ challenged Escherichia coli and chickens fed basal diet + 2.5% garlic powder +ZnO 180 ppm/ challenged Escherichia coli showed performances better than control .Keywords: Sweet potato leaves, Body weight, Consumtion ration, Convertion ration, Broiler

scholarly journals PENGARUH PEMBERIAN RANSUM KOMBINASI SUPLEMEN HERBAL DENGAN MINERAL ZINK TERHADAP JUMLAH LEUKOSIT, ERITROSIT, DAN KADAR HEMOGLOBIN BROILER YANG DIINFEKSI ESCHERICHIA COLI

ZOOTEC ◽  
2014 ◽  
Vol 34 (2) ◽  
pp. 82
Author(s):  
M. R. Regar ◽  
R. Mutia ◽  
S. D. Widhyari ◽  
Y. H.S. Kowel
Keyword(s):  
Escherichia Coli ◽  
Basal Diet ◽  
Positive Control ◽  
Negative Control ◽  
Hemoglobin Level ◽  
Herbal Supplement ◽  
Broiler Performance ◽  
Garlic Powder ◽  
Control Chick ◽  
Ad Libitum

EFFECT OF COMBINATION BETWEEN HERBAL SUPPLEMENT AND ZINC MINERALS IN RATION ON TOTAL OF LEUCOCYT, ERYTHROCYTE, AND HEMOGLOBIN LEVEL IN BROILERS INFECTED BY ESCHERICHIA COLI. This experiment was conducted to study the combination of herbal material and zink in poultry diet on  the leucocyte, erythrocyte and hemoglobin of broilers infected by the amount of Escherichia coli. Two hundred of unsexed d.o.c broilers were devided into five treatments and four replications in each treatment, with five chicks in each replicate. The treatments were basal diet as a negative control/ healhty chickens (R1), basal diet as a positive control, chick was infected by Escherichia coli (R2), basal diet + 1.5% turmeric powder + ZnO 180 ppm, chick was infected by Escherichia coli (R3) , basal diet + 2.5% garlic powder + ZnO 180 ppm,chick was infected by Escherichia coli (R4), and basal diet + antibiotic, chick was infected by Escherichia coli (R5). Data were collected during 35 days. Diet and water were offered ad libitum. The results of this research indicated that chicken fed basal diet + 1.5% turmeric powder + ZnO 180 ppm, chick was infected by Escherichia coli (R3) and chickens fed basal diet + 2.5% garlic powder +ZnO 180 ppm chick was infected by Escherichia coli (R4) showed the best leucocyte, erythrocyte and hemoglobin amount compared with control. Key words: Herbal supplement, zinc mineral, broiler performance.

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