Influence of condensed tannins on gut morphology in sheep fed Lotus pedunculatus

2001 ◽  
Vol 81 (4) ◽  
pp. 605-607 ◽  
Author(s):  
J. P. Walton ◽  
G. C. Waghorn ◽  
J. C. Plaizier ◽  
M. Birtles ◽  
B. W. McBride
Keyword(s):  
Condensed Tannins ◽  
Dry Matter ◽  
Intestinal Morphology ◽  
Feeding Trial ◽  
Gut Morphology ◽  
Lotus Pedunculatus ◽  
Small Intestinal ◽  
Mucosal Morphology ◽  
Effect Of Feeding ◽  
Distal Jejunum

Feeding forages containing condensed tannins (CT) can reduce intestinal absorption of amino acids (AA) and peptides. This experiment tested the hypothesis that CT in Lotus pedunculatus altered small intestinal mucosal morphology reducing absorptive capacity of the gut. Thirteen young Romney wethers were fed ad libitum either Lotus pedunculatus (cv. Grasslands Maku, N = 7) containing 5.5% CT in the dry matter (DM) or white clover-perennial ryegrass pasture (N = 6), which did not contain CT, for 4 wk. The wethers were euthanized at the end of the feeding trial and samples were collected for histological examination from the proximal and distal duodenum, proximal, mid-, and distal jejunum and ileum, and ventral rumen. There was no effect of feeding L. pedunculatus containing 5.5% CT (P > 0.05) on rumen or gut morphological parameters in sheep. The cause of reduced AA absorption in the presence of CT is not known, but this study suggests it is not due to changes in intestinal morphology. Key words: Lotus pedunculatus, condensed tannins, gut morphology, sheep

Effects of condensed tannins in Lotus pedunculatus on its nutritive value for sheep. 1. Non-nitrogenous aspects

1994 ◽  
Vol 123 (1) ◽  
pp. 99-107 ◽  
Author(s):  
G. C. Waghorn ◽  
I. D. Shelton ◽  
W. C. McNabb
Keyword(s):  
Condensed Tannins ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Nutritive Value ◽  
High Fertility ◽  
Feeding Trial ◽  
Molar Ratios ◽  
Rumen Degradation ◽  
Outflow Rate ◽  
Lotus Pedunculatus

SummaryLotus pedunculatus was grown under high fertility conditions and its nutritive value was determined in a feeding trial with sheep at Palmerston North, New Zealand in 1989. The condensed tannins (CT) accounted for 5·5 % of lotus dry matter (DM) and its effect on digestion was evaluated by giving an intraruminal infusion of polyethylene glycol (PEG) to six of the sheep (PEG group). PEG preferentially binds with CT so that the lotus becomes essentially CT-free.The experiment was carried out with 14 sheep (six PEG and eight ‘Tannin’) held in metabolism crates indoors and given freshly cut lotus hourly, for 32 days. This paper presents data relating to carbohydrate and mineral digestion, together with aspects of rumen function.Digestibility of lotus DM was 68%, and the digestibility of fibre was not affected by CT. Infusion of PEG increased rumen concentrations of NH3 and volatile fatty acids (P < 0·001) but effects on molar ratios of VFA were inconsistent with time. CT reduced rumen degradation and absorption of sulphur and increased net absorption of both phosphorus and zinc, but other effects on mineral digestion were small.Although the lotus was offered at c. 90% of ad libitum, intakes of the tannin sheep began to decline after c. 15 days of feeding and were c. 12% lower than those of the PEG sheep at the end of the trial (P < 0·05). At slaughter, rumen pool sizes were similar for the two treatments but the Tannin sheep had a lower fractional outflow rate, which suggests a slower rate of digestion in the rumen. Growth rate and wool production were similar for sheep on both treatments. It is concluded that the CT in Lotus pedunculatus grown under high fertility conditions had little effect on fibre and mineral digestion but the depression in DM intake reduced its nutritive value for sheep.

Effect of condensed tannins inLotus pedunculatuson the nutritive value of ryegrass (Lolium perenne) fed to sheep

1995 ◽  
Vol 125 (2) ◽  
pp. 291-297 ◽  
Author(s):  
G. C. Waghorn ◽  
I. D. Shelton
Keyword(s):  
Lolium Perenne ◽  
Condensed Tannins ◽  
Volatile Fatty Acids ◽  
Nutritive Value ◽  
Major Effect ◽  
Substantial Effect ◽  
Feeding Trial ◽  
Plasma Urea ◽  
Principal Finding ◽  
Lotus Pedunculatus

SUMMARYA feeding trial was undertaken in Palmerston North, New Zealand in 1991, to determine the extent to which condensed tannins (CT) inLotus pedunculatuswere able to affect the nutritive value of ryegrass (which does not contain CT) for sheep. The trial involved three groups of 11 male cryptorchid sheep held in metabolism crates so that intakes, digestibilities and nitrogen balances could be measured over a 42-day feeding period. One group was fed freshly cut ryegrass (Lolium perenneL.) as a sole diet (Grass group) and the other two groups were given a mixture of about one third freshly cut lotus and two thirds ryegrass (dry matter (DM) basis). This mixture containedc. 1·8% CT in the DM. One group given the mixed diet was drenched twice daily with polyethylene glycol (PEG; PEG group) to bind and remove the effects of the CT from the diet, whilst those not drenched were designated the ‘Tannin’ group. Intakes of all groups were constrained to that of the Grass group of sheep.Condensed tannins in the lotus had a major effect on the digestion of ryegrass. When the forages were immature, apparent digestibility of nitrogen (N) was substantially reduced in the Tannin sheep (65·3%)compared to the PEG (77·5%) and Grass (77·9%) groups (P< 0·001). With mature forages, the respective values were 48·8, 62·5 and 53·7% (P< 0·01). Rumen ammonia concentrations and plasma urea concentrations were also reduced by CT. Condensed tannins reduced DM digestibility by 3·7 percentage units (P< 0·05) mainly through the effects on N digestion. Concentrations of acetate, propionate and butyrate in rumen liquor were not affected by treatment, but CT reduced the proportions of the minor volatile fatty acids (VFA; isobutyrate, valerate and isovalerate). Treatment did not affect liveweight gain (131—164 g/day), or wool growth from mid-side patches, and N retention was not reduced by CT, so that the nutritive value of the forage was maintained despite the reduction in N digestibility. The principal finding was that CT inLotus pedunculatuswere able to affect the digestibility of both grass and lotus when fed together, and that as little as 1·8% of CT in the dietary DM had a substantial effect on rumen function.

Effect of condensed tannins in Lotus corniculatus on the nutritive value of pasture for sheep

1997 ◽  
Vol 128 (3) ◽  
pp. 365-372 ◽  
Author(s):  
G. C. WAGHORN ◽  
I. D. SHELTON
Keyword(s):  
Soluble Protein ◽  
Condensed Tannins ◽  
Dry Matter ◽  
Nutritive Value ◽  
Experimental Period ◽  
Lotus Corniculatus ◽  
Feeding Trial ◽  
Wool Growth

The extent to which condensed tannins (CT) in Lotus corniculatus were able to affect protein degradation and the nutritive value of pasture were evaluated at Palmerston North, New Zealand in 1992 in an indoor feeding trial with sheep and using in vitro incubations. The feeding trial involved three groups of seven young wether sheep held indoors in metabolism crates for the 32-day experimental period with one group fed freshly cut ryegrass/clover pasture (Pasture group) whilst the other two groups received a mixture of c. 37% freshly cut Lotus corniculatus and 63% pasture (dry matter (DM) basis). One of the latter groups was given twice daily oral drenches of polyethylene glycol (PEG) to bind with and remove the effects of the CT (PEG group) whilst the remaining sheep (Tannin group) were not drenched. Intakes of sheep given the mixed diets were constrained to that of the pasture group. The CT concentration in the lotus was 26·1 g/kg DM so that the mixed diets contained c. 10g CT/kg DM. Effects of CT on digestion and sheep performance were minimal. Apparent digestibility of N was lower in the Tannin group (76·4%) than PEG (79·8%) or Pasture (79·4%) sheep (P<0·001), but there were no differences in DM digestibility (75%) or wool growth from mid-side patches. Rumen ammonia and soluble protein concentrations appeared to be similar in PEG and Tannin sheep and volatile fatty acid (VFA) concentrations were similar in all groups (100 mmol/l).For incubations in vitro, several ratios of Lotus corniculatus: pasture were used to determine the effects of CT on precipitation of soluble proteins and the net yield of ammonia and gas with and without PEG. Homogenates of Lotus corniculatus leaves and pasture showed that 50% of soluble protein was precipitated by 1·3% CT in homogenate DM and there was a substantial decrease in in vitro degradation to ammonia when lotus leaf accounted for 33% or more of DM with pasture. This work emphasises the need for caution when extrapolating from in vitro to the in vivo situation and suggests that 1% CT from Lotus corniculatus may be insufficient to affect the nutritive value of fresh forages for sheep.

Small intestinal morphology and bacterial populations in ileal digesta and feces of newly weaned pigs receiving a high dietary level of zinc oxide

2001 ◽  
Vol 81 (4) ◽  
pp. 511-516 ◽  
Author(s):  
B. T. Li ◽  
A. G. Van Kessel ◽  
W. R. Caine ◽  
S. X. Huang ◽  
R. N. Kirkwood
Keyword(s):  
Zinc Oxide ◽  
Small Intestine ◽  
Intestinal Morphology ◽  
Weaned Pigs ◽  
Bacterial Populations ◽  
Crypt Depth ◽  
Post Surgery ◽  
Mucosal Thickness ◽  
Small Intestinal ◽  
Mucosal Morphology

A study was carried out to determine the effect of dietary supplementation of 3000 mg kg–1 zinc oxide (ZnO) on the small intestinal morphology and populations of enterobacteriaceae, lactobacilli and clostridia in ileal digesta and feces of weaned pigs. At 17 d of age, 36 pigs from nine litters were fitted with simple T-cannulae at the distal ileum and after a 2-h post-surgery recovery returned to their sows. At 21 d of age, the pigs were weaned and housed in individual metabolism crates. Pigs were allocated to receive a standard starter diet supplemented with or without 3000 mg kg–1 of ZnO. Ileal digesta and fecal samples were collected immediately before weaning and then on days 2, 4, 7, 9, and 11 after weaning and were used to quantify enterobacteriaceae, lactobacilli and clostridial populations by colony enumeration on selective media. Pigs were euthanized following the final sampling, and 2 cm sections of tissue were collected from sites 25, 50 and 75% along the length of the small intestine for determination of mucosal thickness (MT), crypt depth (CD), villous height (VH) and villous width (VW). Zinc oxide supplementation altered the mucosal morphology of the small intestine. Mucosal thickness (P < 0.08) and VH (P < 0.05) were increased at sites 25 and 50% along the length of the small intestine by ZnO supplementation. Overall VW also increased (P < 0.01) with ZnO supplementation. Crypt depth decreased (P < 0.05) at 75% along the length of the small intestine with ZnO supplementation. The ratio of VH to CD was higher (P < 0.05) for ZnO-supplemented than for control-fed pigs at sites 25, 50 and 75% along the length of the small intestine. There was no effect (P > 0.05) of supplementary ZnO on bacterial populations in ileal digesta or feces. The present study indicates that supplementing ZnO in starter diets changes the epithelial morphology of the small intestine, which may affect nutrient digestion and absorption in newly weaned pigs. Key words: Pigs, zinc oxide, bacteria, intestinal morphology

scholarly journals The effect of feeding frequency and meal size on amounts of total and parotid saliva secreted by sheep

1990 ◽  
Vol 63 (2) ◽  
pp. 305-318 ◽  
Author(s):  
Richard R. Carter ◽  
O. Brian Allen ◽  
W. Larry Grovum
Keyword(s):  
Dry Matter ◽  
Feeding Frequency ◽  
Parotid Saliva ◽  
Fixed Amount ◽  
Sham Feeding ◽  
Time Period ◽  
Production Response ◽  
Stem Leaf ◽  
Effect Of Feeding ◽  
Saliva Production

Total and unilateral parotid saliva production during eating were measured in response to offering sheep a fixed amount of lucerne (Medicago sativa) hay as one, two, four or eight meals. Total saliva measurements were obtained using sham-fed oesophageal-fistulated sheep. Unilateral parotid saliva was collected from sheep fitted with reversible re-entrant cannulas. Dry matter intakes and eating times were measured for each meal but were not constrained to particular values. Total and unilateral parotid saliva production during eating increased linearly with the log of the number of meals (p = 0.0001). The amounts corresponding to one, two, four and eight meals of lucerne hay were 1553, 1737, 1851 and 2087 ml during total collections and 209, 248, 307 and 352 ml during unilateral parotid collections. The time-period spent eating and the amount of food consumed both increased as meal number increased. Total saliva collections when lucerne hay was sham-fed as one, two, four or eight meals were associated with eating times of 56.9, 57.4, 70.8 and 86.0 min and intakes of 562, 622, 629 and 638 g dry matter respectively. For unilateral parotid collections, eating times and dry matter intakes were 64.2, 71.3, 78.0, 82.1 min and 515, 579, 614 and 627 g for one, two, four and eight meals of lucerne hay respectively. The saliva production response appeared to be determined through the effects of the time-period spent eating and amounts consumed, but other undetermined effects of feeding frequency contributed to the response. The importance of meal duration on total saliva production was assessed by sham-feeding of 800 g lucerne as stem, leaf, hay, chopped hay or ground and pelleted hay. Increasing meal duration by feeding with stems resulted in the production of 1808 ml saliva, whereas the rapid consumption of pellets resulted in only 442 ml being produced.

scholarly journals A Grain-Based SARA Challenge Affects the Composition of Epimural and Mucosa-Associated Bacterial Communities throughout the Digestive Tract of Dairy Cows

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1658
Author(s):  
Jan C. Plaizier ◽  
Anne-Mette Danscher ◽  
Paula A. Azevedo ◽  
Hooman Derakhshani ◽  
Pia H. Andersen ◽  
...  
Keyword(s):  
Digestive Tract ◽  
Relative Abundance ◽  
Bacterial Communities ◽  
Dry Matter ◽  
Control Diet ◽  
Rrna Gene ◽  
Ruminal Acidosis ◽  
Clustering Patterns ◽  
The 16S Rrna Gene ◽  
Distal Jejunum

The effects of a subacute ruminal acidosis (SARA) challenge on the composition of epimural and mucosa-associated bacterial communities throughout the digestive tract were determined in eight non-lactating Holstein cows. Treatments included feeding a control diet containing 19.6% dry matter (DM) starch and a SARA-challenge diet containing 33.3% DM starch for two days after a 4-day grain step-up. Subsequently, epithelial samples from the rumen and mucosa samples from the duodenum, proximal, middle and distal jejunum, ileum, cecum and colon were collected. Extracted DNA from these samples were analyzed using MiSeq Illumina sequencing of the V4 region of the 16S rRNA gene. Distinct clustering patterns for each diet existed for all sites. The SARA challenge decreased microbial diversity at all sites, with the exception of the middle jejunum. The SARA challenge also affected the relative abundances of several major phyla and genera at all sites but the magnitude of these effects differed among sites. In the rumen and colon, the largest effects were an increase in the relative abundance of Firmicutes and a reduction of Bacteroidetes. In the small intestine, the largest effect was an increase in the relative abundance of Actinobacteria. The grain-based SARA challenge conducted in this study did not only affect the composition and cause dysbiosis of epimural microbiota in the rumen, it also affected the mucosa-associated microbiota in the intestines. To assess the extent of this dysbiosis, its effects on the functionality of these microbiota must be determined in future.

scholarly journals Effect of Dietary Hazelnut Peels on the Contents of Fatty Acids, Cholesterol, Tocopherols, and on the Shelf-Life of Ripened Ewe Cheese

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 538
Author(s):  
Vita Maria Marino ◽  
Teresa Rapisarda ◽  
Margherita Caccamo ◽  
Bernardo Valenti ◽  
Alessandro Priolo ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dry Matter ◽  
Tocopherol Content ◽  
The Other ◽  
Cholesterol Oxidation ◽  
Cholesterol Levels ◽  
Bulk Milk ◽  
Chocolate Industry ◽  
Experimental Group ◽  
Effect Of Feeding

Hazelnut peel (HNP), a by-product from the chocolate industry, is considered to be a suitable ingredient to be included in the diet of ruminants. This study aimed to evaluate the effect of feeding dairy ewes with a diet containing HNP on ripened cheese quality, including fatty acid (FA) profile, cholesterol, and tocopherol content, as well as stability during storage under commercial conditions. In total, 10 experimental cheeses were produced with bulk milk obtained from ewes fed a commercial concentrate (C group; n = 5) or a concentrate containing 36% HNP in dry matter (HNP group; n = 5). After 40 days of aging, each cheese was sub-sampled into three slices: one was analyzed immediately (C0 and HNP0), and the other two were refrigerated and analyzed after seven days (C7 and HNP7) and 14 days (C14 and HNP14), respectively. Compared to C, HNP cheese had more than twice as many tocopherols and mono-unsaturated FA and respectively 38% and 24% less of cholesterol and saturated FA. Tocopherols and cholesterol levels remained rather stable up to 14 days of storage regardless of the experimental group, suggesting no cholesterol oxidation. Therefore, the inclusion of HNP in ewe diets could be a valid resource to produce cheese with a healthier lipid profile and higher tocopherols content.

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