Effects of feeding different levels of conjugated linoleic acid and total oil to pigs on live animal performance and carcass composition

2001 ◽  
Vol 81 (4) ◽  
pp. 505-510 ◽  
Author(s):  
M. E. R. Dugan ◽  
J. L. Aalhus ◽  
K. A. Lien ◽  
A. L. Schaefer ◽  
J. K. G. Kramer

The effects of feeding different dietary levels of conjugated linoleic acid (CLA) and total oil (TO) were evaluated using 216 barrows fed from 36 to 115 kg body weight. A 3 × 2 factorial design was used with three levels of dietary CLA (0, 0.25, and 0.5%), two levels of dietary TO (2 and 5% made up with canola oil) and 12 pens of three barrows fed per diet. Pigs on all diets gained weight at the same rate from 0 to 4 wk (36 to 61 kg) and from 4 wk to slaughter (61 kg to 115 kg) (P > 0.05). Pigs fed 5% versus 2% TO had 6.6% lower feed intake from 0 to 4 wk (P = 0.02) and 5.9% lower feed intake from 4 wk to slaughter (P < 0.003), while feeding CLA had no effect on feed intake (P = 0.70). A CLA by TO interaction for feed intake was not found for 0 to 4 wk but from 4 wk to slaughter a tendency for an interaction (P = 0.06) was found indicating 0.25% CLA may depress feed intake at 2% TO while stimulating intake at 5% TO. Increasing TO and CLA increased the lean content in commercial cuts by 1.6% (P = 0.06) and 2.7% (P = 0.008), respectively. A CLA by TO interaction (P = 0.012), however, indicated CLA was less effective at increasing lean when added to 5% TO diets than to 2% TO diets, with pigs fed the 0% CLA/2% TO diet yielding 5% less lean than pigs fed any of the other diets (P < 0.05). Interestingly, a main effect for TO was not found for subcutaneous fat (SCF), but adding CLA reduced SCF (6.6%; P = 0.002). Again, a CLA by TO interaction (P = 0.008) indicated adding CLA was less effective at reducing SCF in 5% TO than 2% TO diets, with pigs fed the 0% CLA/2% TO diet having 9.7% more SCF than pigs fed any of the other diets (P < 0.05). The difference in effectiveness of CLA at different TO levels might be due to dilution of CLA by canola oil fatty acids and/or canola oil having fat to lean repartitioning effects of its own. A comparison of fats and oils with differing chain lengths and degrees of saturation would, therefore, be of interest to further define their potential differential effects on growth and metabolism. Key words: Pig, pork, swine, conjugated linoleic acid, CLA, canola oil

2003 ◽  
Vol 83 (4) ◽  
pp. 713-720 ◽  
Author(s):  
M. E. R. Dugan ◽  
J. L. Aalhus ◽  
D. C. Rolland ◽  
L. E. Jeremiah

The objective of the present study was to compare pork quality, composition and palatability of pigs fed different levels of conjugated linoleic acid (CLA) and total oil (TO). A 3 × 2 factorial design was used feeding three levels of CLA (0, 0.25, and 0. 5%) and two levels of TO (2 and 5% made up with canola oil). Thirty-six pigs were allotted per diet and diets were fed from 35 to 115 kg liveweight. TO had no effect on longissimus thoracis (LT) moisture or intramuscular fat contents, but feeding CLA decreased LT moisture (P = 0.04) and increased LT intramuscular fat (P = 0.04). CLA and TO had no effect on LT subjective color, structure or marbling scores. Post-mortem LT temperature was also unaffected by feeding CLA or TO. Pigs fed 0.5% CLA had a marginally higher muscle pH (P = 0.01), a tendency toward lower post-mortem lactate levels (P = 0.06) and had higher glycogen concentrations (P = 0.03) at 30 min post mortem. Feeding CLA may thus offer limited protection against rapid post-mortem pH decline. Adding CLA to the diet increased subcutaneous fat hardness (P = 0.01), which was related to increased saturated (P = 0.01) and reduced monounsaturated fatty acid levels (P = 0.01). Increasing TO had an opposite effect resulting in softer fat with lower saturated and higher mono- and polyunsaturated fatty acid levels (P = 0.01). Supplementing diets with CLA in combination with canola oil did not have any detrimental effect on pork quality, composition or palatability. Feeding CLA does, however, have the potential to improve pork quality by increasing intramuscular fat levels, reducing post-mortem glycogen utilization rates and increasing subcutaneous fat hardness. Key words: Conjugated linoleic acid, canola oil, pork quality, palatability, marbling, fat composition


2008 ◽  
Vol 60 (6) ◽  
pp. 1388-1398 ◽  
Author(s):  
S.F. Zanini ◽  
E. Vicente ◽  
G.L. Colnago ◽  
B.M.S. Pessotti ◽  
M.A. Silva

The effect of dietary conjugated linoleic acid (CLA) in association with two vegetable oil sources on the fatty acids of meat and giblets of broiler chickens was evaluated. Two hundred 21-day-old broiler chickens were distributed in a completely randomized factorial design 2 x 5 (two oil sources, soybean or canola oil; and five levels of CLA, 0.0, 2.5, 5.0, 7.5, and 10.0g/kg). The addition of CLA to the diet resulted in an increase (P<0.05) in CLA deposition in the analyzed tissues. CLA supplementation also reduced (P<0.05) the rate of polyunsaturated to saturated fatty acids in thigh, breast, heart, and gizzard. There was interaction of CLA x oil source (P<0.05). The intake of soybean oil, associated with increasing CLA, resulted in an increase in lipid deposition in edible portions as observed by an increase in the overall content of fatty acids, including CLA, while the use of canola oil, associated with increasing CLA in the diet, resulted in a decrease in lipid content in edible portions, specifically regarding that of saturated fat (P<0.05) in breast meat and liver and in the content of monounsaturated fatty acids (P<0.05) in thigh, breast, liver, and gizzard.


2008 ◽  
Vol 75 (3) ◽  
pp. 354-356 ◽  
Author(s):  
Michael R F Lee ◽  
John K S Tweed

This study investigated the evolution of trans-9 trans-11 conjugated linoleic acid (CLA) from cis-9 trans-11 CLA during methylation and its avoidance through a rapid base methylation of milk fat. The study examined three conditions shown to result in loss of cis-9 trans-11 CLA during methylation namely: temperature, methylation time, water contamination in old reagents and acidic conditions. Three techniques currently used for the conversion of milk fat into fatty acid methyl esters for analysis of CLA content by gas liquid chromatography and a fourth procedure designed to eliminate acidic conditions and to limit methylation temperature and time were used. The four methods were: (i) acidic methylation (AM); (ii) acidic and basic bimethylation with fresh reagents (FBM); (iii) acidic and basic bimethylation with pre-prepared reagents (PBM) and (iv) basic methylation (BM). Each regime was carried out on six milk samples over two periods and methylated 1 ml freeze-dried milk (n=12 per regime). Total CLA was not different across methylation regimes (0·30 mg/ml). Isomer cis-9 trans-11 was higher (P<0·01) with BM than the other regimes and lowest with AM: 21·2, 17·8, 18·8 and 14·7 mg/100 ml for BM, FBM, PBM and AM, respectively. The inverse relationship was shown for trans-9 trans-11 with higher (P<0·001) amounts with AM than the other regimes and lowest with BM: 0·57, 2·55, 2·36 and 3·69 mg/100 ml for BM, FBM, PBM and AM, respectively. The trans-10 cis-12 isomer was also shown to alter with methylation procedure being higher (P<0·001) with AM than the other regimes: 0·43, 0·47, 0·29 and 1·20 mg/100 ml for BM, FBM, PBM and AM, respectively. Validation with known CLA free fatty acid and triacylglycerol standards confirmed that AM resulted in conversion of cis-9 trans-11 to trans-9 trans-11, and also elevated trans-10 cis-12 whilst BM of triacylglycerol CLA did not isomerise cis-9 trans-11 and was comparable to FBM.


2011 ◽  
Vol 51 (No. 2) ◽  
pp. 47-53 ◽  
Author(s):  
L. Bartoň ◽  
D. Řehák ◽  
V. Teslík ◽  
D. Bureš ◽  
R. Zahrádková

Breed effects on live weight gain, slaughter characteristics and carcass composition were compared in Aberdeen Angus, Charolais, Hereford and Simmental bulls. The experiment extended over 2 years and involved totally 96 animals. The target slaughter live weights were determined 550 kg for earlier maturing breeds Aberdeen Angus and Hereford and 630 kg for later maturing breeds Charolais and Simmental. Charolais and Simmental gained more rapidly (P &lt; 0.05) than Aberdeen Angus while Hereford were intermediate. Hereford had lower (P &lt; 0.05) dressing percentage than the other breeds. Percentages of grade I meat were significantly higher (P &lt; 0.05) in Charolais and Simmental. The highest percentage of separable fat was recorded in Hereford (P &lt; 0.05). Charolais and Simmental had lower (P &lt; 0.05) thickness of subcutaneous fat over MLLT than Aberdeen Angus andHereford. The later maturing bulls generally tended to achieve higher live weight gains during the experiment, produced less fat and had higher percentage of meat from high priced joints in comparison with earlier maturing animals. &nbsp;


2001 ◽  
Vol 79 (7) ◽  
pp. 1821 ◽  
Author(s):  
R L Thiel-Cooper ◽  
F C Parrish ◽  
J C Sparks ◽  
B R Wiegand ◽  
R C Ewan

1999 ◽  
Vol 69 (3) ◽  
pp. 613-625 ◽  
Author(s):  
N. W. Offer ◽  
M. Marsden ◽  
J. Dixon ◽  
B. K. Speake ◽  
F. E. Thacker

AbstractThe effects of three fat supplements on milk yield and composition were measured using 12 mid-lactation in-calf Hoistein-Friesian cows in a balanced incomplete change-over design over three periods each of 3 weeks. All cows received a basal diet consisting of 36 kg/day grass silage (dry matter (DM) 270 g/kg, metabolizable energy (ME) 11·6 MJ/kg DM) and 7 kg/day o f a concentrate mixture containing (g/kg) rolled barley (501), molassed sugar-beet pulp shreds (277), soya-bean meal (208) and a standard cow mineral supplement (14). Treatments were CON (control-no supplement); LIN and FISH (250 gl day of either linseed oil or marine oil, providing approximately 0·046 of ME intake) or TOA (95 glday of tuna orbital oil, providing 0·018 of total ME intake).There were no significant effects on silage DM intake or milk yield (means 9·25 and 17·2 kg/day respectively). The FISH and TOA treatments depressed (F < 0·05) milk fat concentration (45·4, 44·6, 34·5 and 41·6 (s.e.d. 1·08) g/kg for CON, LIN, FISH and TOA respectively; note — the same treatment order is used for all results quoted). Compared with values for CON, yield of f at (glday) was significantly (F < 0·05) greater for LIN and significantly lower for FISH (739, 808, 572 and 732, s.e.d. 28·7). All three oil supplements reduced (F < 0·05) milk protein content (33·6, 32·5, 30·6 and 32·4 (s.e.d. 0·43) g/kg) but, apart from a small increase for LIN, protein yield (glday) was unaffected (545, 586, 510 and 574, s.e.d. 20·2).The concentrations (g/100 g) of short-chain fatty acids (< C14) and C16 : 0 in milk f at were lower (F < 0·05) for LIN than for the other treatments. All supplements increased the concentrations ofC18:1 (F < 0·05), the value for LIN being greater (F < 0·05) than for the other treatments (21·0, 27·2, 25·3 and 23·7, s.e.d. 0·74). The FISH and TOA treatments increased (F < 0·05) the concentrations of long chain (< C2O) (n-3) poly-unsaturated fatty acids (PUFA), (0·19, 0·17, 0·49 and 0·27, s.e.d. 0·026) but less than proportionately 0·03 of dietary intake of these acids was transferred to milk, probably because they were found to be mostly in the phospholipid and cholesterol ester fractions of plasma. The FISH and TOA treatments increased (F < 0·05) the percentages of total trans fatty acids in milk fat (1·13, 2·19, 10·26 and 3·62, s.e.d. 0·728) whilst a significant (F < 0·05) increase in conjugated linoleic acid (CLA) was observed only for FISH (0·16, 0·28, 1·55, and 0·52, s.e.d. 0·154). Concentrations of CLA and total trans acids in milk were highly correlated (r = 0·91, no. =36, F < 0·001) whilst trans acids in milk were inversely correlated with milk fat content (r = -0·63, no. = 36, F < 0·001) supporting the theory that milk fat depression may be caused by increased supply of trans fatty acids to the mammary gland. The health implications of these changes in milk fat composition are discussed.


1997 ◽  
Vol 77 (4) ◽  
pp. 723-725 ◽  
Author(s):  
M. E. R. Dugan ◽  
J. L. Aalhus ◽  
A. L. Schaefer ◽  
J. K. G. Kramer

The repartioning effect of CLA was evaluated using 108 pigs (54 gilts and 54 barrows) fed from 61.5 to 106 kg liveweight. Pigs were fed a cereal-based basal diet containing either 2% CLA or 2% sunflower oil (a rich source of α-linoleic acid). Irrespective of gender, pigs fed CLA tended to have reduced feed intakes (−5.2%, P = 0.07), improved feed conversion efficiences (−5.9%, P = 0.06) and similar rates of gain relative to sunflower oil fed pigs. In addition, pigs fed CLA deposited less subcutaneous fat (−6.8%, P = 0.01) and gained more lean (+2.3%, P = 0.03) than pigs fed sunflower oil. These data suggest CLA can be used as a repartioning agent in pigs and that further research to optimize the repartitioning effect is warranted. Key words: Pig, pork, swine, conjugated linoleic acid, CLA, repartioning


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