scholarly journals Proteomic Analysis of Soybean [Glycine max (L.) Merrill] Roots Inoculated with Bradyrhizobium japonicum Strain CPAC 15

2013 ◽  
Vol 6 ◽  
pp. PRI.S13288 ◽  
Author(s):  
Adalgisa R. Torres ◽  
Elisete P. Rodrigues ◽  
Jesiane S.S. Batista ◽  
Douglas F. Gomes ◽  
Mariangela Hungria
Keyword(s):  
Proteomic Analysis ◽  
Bradyrhizobium Japonicum ◽  
Pyridine Nucleotide ◽  
Rubisco Activase ◽  
Information Storage ◽  
Functional Categories ◽  
Glutathione S Transferase ◽  
Cellular Processes ◽  
Glycine Max L ◽  
Commercial Inoculants

This research intended to analyze the expression pattern of proteins in roots of the Brazilian soybean cultivar Conquista when inoculated with Bradyrhizobium japonicum CPAC 15, a strain broadly used in commercial inoculants in Brazil. At ten days after bacterial inoculation, whole-cell proteins were extracted from roots and separated by 2-D gel electrophoresis. Comparative analysis revealed significant changes in the intensity of 37 spots due to the inoculation (17 up-regulated and 20 down-regulated proteins), identified by MALDI-TOF/TOF-TOF. Identified proteins were associated with COG functional categories of information storage and processing, cellular processes and signaling, metabolism, and also in the “poorly characterized” and “not in COG” categories. Among the up-regulated proteins, we identified sucrose synthase (nodulin-100), β-tubulin, rubisco activase, glutathione-S-transferase, a putative heat-shock 70-kDa protein, pyridine nucleotide-disulphideoxidoreductase and a putative transposase. Proteomic analysis allowed for the identification of some putative symbiotic functions and confirmed the main biological processes triggered in the nitrogen-fixing symbiosis with soybean.

Effect of numbers of Bradyrhizobium japonicum applied in commercial inoculants on soybean seed yield in Ontario

1992 ◽  
Vol 38 (6) ◽  
pp. 588-593 ◽  
Author(s):  
D. J. Hume ◽  
D. H. Blair
Keyword(s):  
Glycine Max ◽  
Seed Yield ◽  
Bradyrhizobium Japonicum ◽  
Field Experiments ◽  
Soybean Seed ◽  
Yield Response ◽  
Cubic Equation ◽  
Glycine Max L ◽  
Seed Yields ◽  
Commercial Inoculants

In the absence of Bradyrhizobium japonicum populations in the soil, yields of field-grown soybean (Glycine max (L.) Merrill) usually respond to inoculation with B. japonicum. The objective of this research was to determine the relationship between numbers of B. japonicum per seed in inoculants and soybean nodulation and yield. A total of six field experiments were conducted in 1989 and 1990 on new soybean soils. In dilution trials, Grip inoculant was applied to provide approximately 106, 105, 104, and 103B. japonicum per seed at two locations in 1989. Nodule number and mass, as well as seed yield, increased curvilinearly upward with increasing log10 most probable numbers (MPNs) of B. japonicum. The yield response curve was best fit by a cubic equation, which accounted for 97% of the variation in yield. Seed yields increased 19% (1.83 to 2.13 Mg/ha) from 105 to 106B. japonicum per seed. In field experiments involving 8 commercial inoculants in 1989 and 10 in 1990, and conducted at two locations in each year, responses to increasing log MPNs in the inoculants also were concave upwards and cubic. In the two years, 78 and 46% of the yield variation was accounted for by log MPN per seed. Increasing MPN per seed from 105 to 106 improved yields in first-time fields by an average of 24%, indicating the present minimum standard of 105B. japonicum per seed should be increased. Key words: most probable numbers, response to inoculation, nodulation, Glycine max (L.) Merrill.

Short-term recovery of Bradyrhizobium japonicum during an inoculation process using mineral microgranules

1994 ◽  
Vol 40 (4) ◽  
pp. 322-325 ◽  
Author(s):  
Georges Fouilleux ◽  
Cécile Revellin ◽  
Gérard Catroux
Keyword(s):  
Glycine Max ◽  
Moisture Content ◽  
Bradyrhizobium Japonicum ◽  
Water Retention ◽  
Short Term ◽  
Relative Water ◽  
Glycine Max L ◽  
Retention Characteristics ◽  
Granule Type ◽  
Commercial Inoculants

Soybean (Glycine max (L.) Merr.) can be inoculated by delivering inoculant mixed with mineral microgranules to the seedbed. An average decline of 0.61 log units in the number of viable Bradyrhizobium japonicum was found during the 1sth, using available commercial inoculants and granules. These losses were shown to be influenced by inoculant type (peat based, liquid, lyophilized) and granule type. When mixed with granules, a peat-based inoculant was found to be desiccated immediately and subjected to a water potential as low as −170 MPa. Bradyrhizobium japonicum recovery was improved when the moisture content of the granules increased. It was concluded that water suction by granules can subject the bacteria to rapid desiccation and significantly decrease their number. Thus, the nature of inoculant and granular material and their relative water retention characteristics must be taken into account to improve the efficiency of this inoculation process.Key words: Bradyrhizobium japonicum, granular inoculant, inoculation, soybean, survival.

Metabolic Adjustment of Glycine max (L.) Merril in the Presence of Nitrate and Bradyrhizobium japonicum

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1518
Author(s):  
Alberto Mongolo Júnior ◽  
Felipe Girotto Campos ◽  
Gustavo Ribeiro Barzotto ◽  
Jonas Akenaton Venturineli Pagassini ◽  
Maria Aparecida Ribeiro Vieira ◽  
...  
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Dry Mass ◽  
Antioxidant Systems ◽  
Oxygen Species ◽  
Membrane Stabilization ◽  
Total Sugars ◽  
Vegetative Phase ◽  
Glycine Max L ◽  
Metabolic Adjustment ◽  
Plant Dry Mass

Reactive oxygen species are generated during the processes of photosynthesis and nitrate reduction, which can compromise the integrity of biomolecules and membranes. During the vegetative phase of Fabaceae species, around half of translocated carbohydrate is used for nodule growth, while the other half returns to the aerial part with nitrogen incorporated. These sugars may be yet involved with membrane stabilization, signaling, and activation of important genetic pathways for plant development. Thus, the aim was to study the adjustments of the photosynthetic and antioxidant systems and the accumulation of carbohydrates and biomass in Glycine–Bradyrhizobium cultivated with nitrate (NO3−). Four treatments were evaluated in completely randomized blocks. Glycine–Bradyrhizobium was grown with 1.7 mM of NO3− (GB: 1.7 mM NO3−) and without NO3− (GB: 0 mM NO3−), and Glycine was grown with 1.7 mM of NO3− (G: 1.7 mM NO3−) and without NO3− (G: 0 mM NO3−). Glycine–Bradyrhizobium symbiosis contributes to photosynthetic metabolism and total sugars, reduces the action of antioxidant enzymes, and minimizes the use of nitrate in soybean cultivation.; Glycine–Bradyrhizobium with nitrate provided greater plant dry mass in the vegetative phase, along with increased enzymatic activity and reduced nodule mass.

scholarly journals Response of Soybean (Glycine max (L.) Merrill) to Mineral Nitrogen Fertilization and Bradyrhizobium japonicum Seed Inoculation

Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1300
Author(s):  
Janusz Prusiński ◽  
Anna Baturo-Cieśniewska ◽  
Magdalena Borowska
Keyword(s):  
Nitrogen Fertilization ◽  
Bradyrhizobium Japonicum ◽  
Plant Density ◽  
Higher Plants ◽  
High Sensitivity ◽  
N Fertilization ◽  
Mineral N ◽  
Plant Nitrogen ◽  
Seed Inoculation ◽  
Glycine Max L

A growing interest in soybean cultivation in Poland has been observed in the recent years, however it faces a lot of difficulties resulting from a poorly understood effectiveness of plant nitrogen fertilization and from the introduction of Bradyrhizobium japonicum to the environment. The aim of the study was to evaluate the consistency of response of two soybean cultivars to three different rates of mineral N fertilization and two seed inoculation treatments with B. japonicum in field conditions over four years regardless of previous B. japonicum presence in the soil. A highly-diversified-over-years rainfall and temperature in the growing season do not allow for a definite statement of the differences resulting from seed inoculation and mineral N fertilization applied separately or jointly in soybean. A high sensitivity of the nodulation process to rainfall deficits was noted, which resulted in a decreased amount of B. japonicum DNA measured in qPCR and dry matter of nodules. ‘Annushka’ demonstrated a higher yield of seeds and protein, higher plants and the 1st pod setting. ‘Aldana’, due to a significant decrease in plant density, produced a higher number of pods, seeds per pod and the 1000 seed weight per plant. Both cultivars responded with an increase in the seed yield after seed inoculation with HiStick, also with an application of 30 and 60 kg N, as well as with Nitragina with 60 kg N.

scholarly journals Amplification of 16S rRNA gene sequences to differentiate two highly related bradyrhizobia species

2007 ◽  
Vol 42 (9) ◽  
pp. 1361-1364 ◽  
Author(s):  
Adriana Giongo ◽  
Adriana Ambrosini ◽  
João Ruy Jardim Freire ◽  
Maria Helena Bodanese Zanettini ◽  
Luciane Maria Pereira Passaglia
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Bradyrhizobium Japonicum ◽  
Diagnostic Method ◽  
Molecular Diagnostic ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Closely Related Species ◽  
Reference Strains ◽  
Commercial Inoculants

A 16S rRNA gene PCR-based assay was developed aiming at a fast molecular diagnostic method to differentiate the two phylogenetically closely related species Bradyrhizobium japonicum and B. elkanii, isolated from soybean nodules, in order to identify those more competitive and comprising greater nitrogen fixation ability for use in the formulation of commercial inoculants. The assay used was able to discriminate ten reference strains belonging to these two Bradyrhizobium species, as well as to efficiently identify 37 strains isolated from fields cultivated with soybean.

scholarly journals Diversidad de los rizobios que nodulan la soja en los suelos de la Pampa húmeda e identificación de cepas para la fabricación de inoculantes comerciales

2016 ◽  
Author(s):  
◽  
Graciela N. Pastorino
Keyword(s):  
Glycine Max ◽  
Bradyrhizobium Japonicum ◽  
Glycine Max L

Las labores culturales, provocan modificaciones de las propiedades físicas, químicas y biológicas de los suelos. Estas se clasifican en: labranza convencional, labranza vertical y siembra directa. Cada tecnología de manejo genera estreses a los que las poblaciones microbianas se adaptan, como resultado de cambios morfológicos, fisiológicos y genéticos. La inoculación de la soja (Glycine max [L.] Merr) es una tecnología que se introdujo en la Argentina junto con el cultivo y por ello los inoculantes fueron el vehículo de ingreso de las cepas exóticas de Bradyrhizobium, que una vez incorporadas al suelo, se adaptaron y establecieron dando origen a las poblaciones de rizobios naturalizadas. Lo que condujo a generar diversidad a nivel del genoma de los bradyrizobios. El objetivo de este trabajo fue evaluar la diversidad de los simbiontes de soja que se encuentran en la población del suelo y el rol que cumple como recurso en la selección de cepas para la producción comercial de inoculantes. Se evaluaron dos muestras de suelo que durante los últimos 8 años fueron trabajados con siembra directa (SD) y con labranza convencional (LC). Se realizó el recuento y aislamiento de rizobios. Los aislados se caracterizaron fisiológica y genéticamente. La diversidad se evaluó mediante la amplificación de las secuencias BOX A1R. Se seleccionaron 52 aislados que se identificaron amplificando las regiones RSα y nifD y la secuenciación del fragmento ITS 16S - 23S rDNA. El recuento de rizobios mostró que en el suelo bajo SD y antecesor soja la población fue mayor que en la muestra de suelo bajo LC y antecesor maíz. La caracterización fisiológica de 200 aislados reflejó que los suelos LC contuvieron un mayor número de cepas tolerantes a salinidad y alta temperatura. Además un porcentaje mayor de aislados del suelo LC, respecto del suelo bajo SD, resultó con capacidad de sobrevivencia sobre semilla superior a la cepa control E109. Los aislados además presentaron diferencias en la nodulación y fijación de nitrógeno, en la tolerancia a glifosato y en la producción de ácido indol acético (AIA). Sólo un aislado mostró capacidad de solubilización de fósforo. El análisis del dendrograma generado con los fingerprints BOX A1R, mostró que hay mayor diversidad en los suelos bajo SD. Sobre los 52 aislados analizados en base a la secuencia del ITS se encontró que el 70 % son Bradyrhizobium japonicum y 30 % B. elkanii. Se identificaron 4 aislados que comparten características genéticas de ambas especies de Bradyrhizobium. En esta tesis se confirmó que en los suelos con historia de cultivo de soja, manejados bajo distintos sistemas de labranzas, se indujeron cambios a nivel del genoma de las cepas de rizobios que contribuyó a modificar la diversidad de la población. Las poblaciones naturalizadas son un recurso para la identificación de rizobios con características superiores para la fabricación de inoculantes, sin embargo es necesario profundizar las evaluaciones de la supervivencia, tolerancia, competitividad y promoción del crecimiento vegetal de rizobios naturalizados para emplearlos como bioinoculantes.

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