Cranberry (Vaccinium Macrocarpon) Changes the surface hydrophobicity and biofilm formation of E. coli

Microbiology Insights ◽

10.4137/mbi.s8526 ◽

2011 ◽

Vol 4 ◽

pp. MBI.S8526 ◽

Cited By ~ 4

Author(s):

Jose Uberos ◽

Ishan Iswaldi ◽

Rocio Rodríguez Belmonte ◽

Antonio Segura-Carretero ◽

Verónica Fernández-Puentes ◽

...

Keyword(s):

Biofilm Formation ◽

Culture Medium ◽

Urinary Infection ◽

Haemagglutination Inhibition ◽

Surface Hydrophobicity ◽

Vaccinium Macrocarpon ◽

Bacterial Suspension ◽

Adhesive Properties ◽

E Coli

Cranberry has been shown useful in the prevention of urinary infection by E. coli. In this study, we examined the changes in the hydrophobicity of P fimbriated E. coli and biofilm formation after incubation with commercial cranberry syrup extract at various concentrations. After incubating a bacterial suspension with cranberry at dilutions of 1:100 and 1:1000, tests of haemagglutination inhibition, surface hydrophobicity and biofilm formation were carried out. The surface hydrophobicity of E. coli decreased significantly after incubation with cranberry and this effect was not modified by the culture medium. Biofilm formation was inhibited after incubation with cranberry syrup and this effect was dependent on the culture medium. Thus, in some circumstances cranberry can modify nonspecific adhesive properties of E. coli. In previous studies, cranberry has only been implicated in the inhibition of P-fimbriated E. coli, but our observations show that it acts by modifying adhesive properties under P-related fimbriae.

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Antibiofilm Activity of an Experimental Ricinus Communis Dentifrice on Soft Denture Liners

Brazilian Dental Journal ◽

10.1590/0103-6440201902326 ◽

2019 ◽

Vol 30 (3) ◽

pp. 252-258 ◽

Cited By ~ 1

Author(s):

Maurício Malheiros Badaró ◽

Vanessa Maria Fagundes Leite-Fernandes ◽

Luciano Trevisan Martin ◽

Viviane de Cássia Oliveira ◽

Evandro Watanabe ◽

...

Keyword(s):

Biofilm Formation ◽

Culture Medium ◽

Solid Medium ◽

Ricinus Communis ◽

Positive Control ◽

Antibiofilm Activity ◽

Candida Spp ◽

E Coli ◽

Liquid Culture Medium ◽

Denture Liners

Abstract The disadvantage of liners materials is the difficulty of biofilm control. It was compared an experimental dentifrice contained Ricinus communis, with commercials dentifrices as antibiofilm activity against microorganisms on denture liner. Six hundred specimens were distributed in 5 groups (n=18/ microorganism): water; experimental dentifrice; specific dentifrice for denture and two conventional dentifrices against C. albicans; C. glabrata; S. mutans; S. aureus; E. coli. Each group had a negative (n=5; without contamination) and positive control (n=15/ microorganism; without cleaning). The antibiofilm activity was evaluated by the method of biofilm formation in triplicate. The specimens were contaminated in a standard way and incubated. After that, manual brushing was performed (60 s), washed with PBS, immersed in liquid culture medium for resuspension and sowing in solid medium. The results (mean of triplicates) were expressed in CFU/mL. The data was submitted to Shapiro-Wilk, ANOVA and Tukey test (p<0.05). The specific dentifrice (1.27±1.20) was the most effective against S. mutans, followed by conventional (Trihydral, 3.13±0.88; Colgate, 2.16±2.02) and experimental (3.81±1.37) dentifrices, which were similar to each other (p=0.008). All of them were different from water (4.79±1.42). The specific (0.21±0.21) and experimental (0.36±0.25) dentifrices were similar against S. aureus, with a higher mean of CFU when compared to conventional (Colgate, 0.06±0.13), which was more efficient (p=0.000). For C. albicans, C. glabrata and E. coli, all dentifrices were similar to water (p=0.186). It was concluded, that the experimental dentifrice was effective against S. aureus and had not efficacy against Candida spp.; S. mutans; E. coli, as occurred with the commercials dentifrices.

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Uropathogenic Escherichia coli Biofilm-Forming Capabilities are not Predictable from Clinical Details or from Colonial Morphology

Diseases ◽

10.3390/diseases8020011 ◽

2020 ◽

Vol 8 (2) ◽

pp. 11 ◽

Cited By ~ 2

Author(s):

Shane Whelan ◽

Mary Claire O’Grady ◽

Dan Corcoran ◽

Karen Finn ◽

Brigid Lucey

Keyword(s):

Escherichia Coli ◽

Biofilm Formation ◽

Culture Medium ◽

Recurrent Infection ◽

Positive Control ◽

Uropathogenic Escherichia Coli ◽

E Coli ◽

Recurrent Uti ◽

Colonial Morphology ◽

The Difference

Antibiotic resistance is increasing to an extent where efficacy is not guaranteed when treating infection. Biofilm formation has been shown to complicate treatment, whereby the formation of biofilm is associated with higher minimum inhibitory concentration values of antibiotic. The objective of the current paper was to determine whether biofilm formation is variable among uropathogenic Escherichia coli isolates and whether formation is associated with recurrent urinary tract infection (UTI), and whether it can be predicted by phenotypic appearance on culture medium A total of 62 E. coli isolates that were reported as the causative agent of UTI were studied (33 from patients denoted as having recurrent UTI and 29 from patients not specified as having recurrent UTI). The biofilm forming capability was determined using a standard microtitre plate method, using E. coli ATCC 25922 as the positive control. The majority of isolates (93.6%) were found to be biofilm formers, whereby 81% were denoted as strong or very strong producers of biofilm when compared to the positive control. Through the use of a Wilcox test, the difference in biofilm forming propensity between the two patient populations was found to not be statistically significant (p = 0.5). Furthermore, it was noted that colony morphology was not a reliable predictor of biofilm-forming propensity. The findings of this study indicate that biofilm formation is very common among uropathogens, and they suggest that the biofilm-forming capability might be considered when treating UTI. Clinical details indicating a recurrent infection were not predictors of biofilm formation.

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Antibacterial activity of isolated phenolic compounds from cranberry (Vaccinium macrocarpon) against Escherichia coli

Food & Function ◽

10.1039/c5fo01441g ◽

2016 ◽

Vol 7 (3) ◽

pp. 1564-1573 ◽

Cited By ~ 24

Author(s):

Celia Rodríguez-Pérez ◽

Rosa Quirantes-Piné ◽

José Uberos ◽

Cecilia Jiménez-Sánchez ◽

Alejandro Peña ◽

...

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Phenolic Compounds ◽

Surface Hydrophobicity ◽

Vaccinium Macrocarpon ◽

E Coli

Apart from proanthocyanidins, isolated polyphenols from cranberry can act againstE. coliadherence and/or modify its surface hydrophobicityin vitro.

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Inhibitory Effect ofBacillus velezensison Biofilm Formation byStreptococcus mutans

10.1101/313965 ◽

2018 ◽

Cited By ~ 1

Author(s):

Yesol Yoo ◽

Dong-Ho Seo ◽

Hyunjin Lee ◽

Young-Do Nam ◽

Myung-Ji Seo

Keyword(s):

Dental Caries ◽

Streptococcus Mutans ◽

Biofilm Formation ◽

Culture Medium ◽

Focal Point ◽

Cell Surface Hydrophobicity ◽

Surface Hydrophobicity ◽

Inhibitory Effect ◽

Fermented Foods ◽

Bacillus Velezensis

ABSTRACTStreptococcus mutansplays a key role in the development of dental caries and promotes the formation of oral biofilm produced by glucosyltransferases (GTFs).Bacillus velezensisK68 was isolated from traditional fermented foods and inhibits biofilm formation mediated byS. mutans. Gene amplification results demonstrated thatB. velezensisK68 contained genes for the biosynthesis of 1-deoxynojirimycin (1-DNJ), a known GTF expression inhibitor. The presence of the GabT1, Yktc1, and GutB1 genes required for 1-DNJ synthesis inB. velezensisK68 was confirmed. Supernatant fromB. velezensisK68 culture medium inhibited biofilm formation by 84% whenS. mutanswas cultured for 48 h, and inhibited it maximally when 1% glucose was added to theS. mutansculture medium as a GTF substrate. In addition, supernatant fromB. velezensisK68 medium containing 3 ppb 1- DNJ decreasedS. mutanscell surface hydrophobicity by 79.0 ± 0.8% compared with that of untreated control. The supernatant containing 1-DNJ decreasedS. mutansadherence by 99.97% and 98.83% under sugar-dependent and sugar-independent conditions, respectively.S. mutanstreated with the supernatant exhibited significantly reduced expression of the essential GTF genesgtfB,gtfC,andgtfDcompared to that in the untreated group. Thus,B. velezensisinhibits the biofilm formation, adhesion, and GTF gene expression ofS. mutansthrough 1- DNJ production.IMPORTANCEDental caries is among the most common infectious diseases worldwide, and its development is closely associated with physiological factors of bacteria, such as the biofilm formation and glucosyltransferase production ofStreptococcus mutans.Biofilms are difficult to remove once they have formed due to the exopolysaccharide matrix produced by the microorganisms residing in them; thus, inhibiting biofilm formation is a current focal point of research into prevention of dental caries. This study describes the inhibitory properties ofBacillus velezensisK68, an organism isolated from traditional Korean fermented foods, against biofilm formation byS. mutans. Herein, we show thatB. velezensisinhibits the biofilm formation, adherence to surfaces, and glucosyltransferase production ofS. mutans.

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Effect of multi-walled carbon nanotubes on Escherichia coli biofilm formation

Вестник Пермского университета. Серия «Биология»=Bulletin of Perm University. Biology ◽

10.17072/1994-9952-2021-2-87-92 ◽

2021 ◽

pp. 87-92

Author(s):

Yu. G. Maksimova ◽

◽

Ya. E. Bykova ◽

◽

Keyword(s):

Escherichia Coli ◽

Carbon Nanotubes ◽

Biofilm Formation ◽

Culture Medium ◽

Nano Materials ◽

E Coli ◽

Multi Walled Carbon Nanotubes ◽

Walled Carbon Nanotubes ◽

Different Sources ◽

Degree Of Purification

The effect of purified and unpurified multi-walled carbon nanotubes on the biofilm formation of Esche-richia coli strains isolated from different sources has been studied. It has been shown that carbon nano-materials in the culture medium do not inhibit biofilm formation, but on days 1–3 of growth lead to the formation of more massive biofilms of some strains. Significantly more intense destruction of mature bio-films of E. coli K12, E. coli K12 TG1 (pXen7) and one natural strain in the presence of carbon nanotubes in the medium was noted. No clear dependence of biofilm formation and destruction of formed biofilms on the degree of purification of nanotubes was found.

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Influence of some parameters on the ability of Listeria monocytogenes, Listeria innocua, and Escherichia coli to form biofilms

Veterinary World ◽

10.14202/vetworld.2019.459-465 ◽

2019 ◽

Vol 12 (3) ◽

pp. 459-465 ◽

Cited By ~ 1

Author(s):

Sara Lezzoum-Atek ◽

Leila Bouayad ◽

Taha Mossadak Hamdi

Keyword(s):

Escherichia Coli ◽

Listeria Monocytogenes ◽

Biofilm Formation ◽

Culture Medium ◽

Staining Technique ◽

Listeria Innocua ◽

E Coli ◽

Key Factor ◽

Polystyrene Support ◽

Multispecies Biofilms

Aim: The present study was conducted to evaluate the capacity of Listeria monocytogenes (L.m), Listeria innocua (L.i), and Escherichia coli to form biofilms on polystyrene support under different parameters by performing crystal violet (CV) staining technique. Materials and Methods: Different suspensions were prepared with single strains and with multiple combinations of strains including two serogroups of L.m (IIa and IIb), L.i, and E. coli strains at different microbial load. Selected strains and combinations were grown in biofilms for 6 days attached to polystyrene microplates under aerobic and microaerophilic conditions. The evaluation of the power of adhesion and biofilm formation was determined by CV staining followed by the measurement of optical density at 24 h, 72 h, and 6 days incubation time with and without renewal of the culture medium. Results: All the strains tested, presented more or less adhesion power depending on the variation of the studied parameters as well as the ability to form multispecies biofilms. Their development is more important by renewing the culture medium and increasing the initial load of bacteria. The ability to adhere and form biofilms differs from one serogroup to another within the same species. In bacterial combination, strains and species of bacteria adopt different behaviors. Conclusion: The ability to form biofilms is a key factor in the persistence of tested strains in the environment. Our study showed that L.m, L.i, and E. coli could adhere to polystyrene and form biofilms under different conditions. More researches are necessary to understand the mechanisms of biofilm formation and the influence of different parameters in their development.

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Biofilm formation by ESBL-producing E. coli and K. pneumoniae

10.26226/morressier.56d5ba30d462b80296c95657 ◽

2016 ◽

Author(s):

Laure Surgers

Keyword(s):

Biofilm Formation ◽

E Coli

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Label-free quantitative proteomic analysis of the inhibition effect of Lactobacillus rhamnosus GG on Escherichia coli biofilm formation in co-culture

Proteome Science ◽

10.1186/s12953-021-00172-0 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Huiyi Song ◽

Ni Lou ◽

Jianjun Liu ◽

Hong Xiang ◽

Dong Shang

Keyword(s):

Escherichia Coli ◽

Proteomic Analysis ◽

Biofilm Formation ◽

Lactobacillus Rhamnosus ◽

Differentially Expressed ◽

Differentially Expressed Proteins ◽

Label Free ◽

Quantitative Proteomic Analysis ◽

E Coli ◽

Protein Ubiquitination

Abstract Background Escherichia coli (E. coli) is the principal pathogen that causes biofilm formation. Biofilms are associated with infectious diseases and antibiotic resistance. This study employed proteomic analysis to identify differentially expressed proteins after coculture of E. coli with Lactobacillus rhamnosus GG (LGG) microcapsules. Methods To explore the relevant protein abundance changes after E. coli and LGG coculture, label-free quantitative proteomic analysis and qRT-PCR were applied to E. coli and LGG microcapsule groups before and after coculture, respectively. Results The proteomic analysis characterised a total of 1655 proteins in E. coli K12MG1655 and 1431 proteins in the LGG. After coculture treatment, there were 262 differentially expressed proteins in E. coli and 291 in LGG. Gene ontology analysis showed that the differentially expressed proteins were mainly related to cellular metabolism, the stress response, transcription and the cell membrane. A protein interaction network and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis indicated that the differentiated proteins were mainly involved in the protein ubiquitination pathway and mitochondrial dysfunction. Conclusions These findings indicated that LGG microcapsules may inhibit E. coli biofilm formation by disrupting metabolic processes, particularly in relation to energy metabolism and stimulus responses, both of which are critical for the growth of LGG. Together, these findings increase our understanding of the interactions between bacteria under coculture conditions.

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Antimicrobial and Immunomodulating Activities of Two Endemic Nepeta Species and Their Major Iridoids Isolated from Natural Sources

Pharmaceuticals ◽

10.3390/ph14050414 ◽

2021 ◽

Vol 14 (5) ◽

pp. 414

Author(s):

Neda Aničić ◽

Uroš Gašić ◽

Feng Lu ◽

Ana Ćirić ◽

Marija Ivanov ◽

...

Keyword(s):

Biofilm Formation ◽

Food Industry ◽

Balkan Peninsula ◽

Antimicrobial Activities ◽

Natural Sources ◽

E Coli ◽

Food Borne ◽

Metabolite Profiles ◽

Aspergillus Sp ◽

First Time

Two Balkan Peninsula endemics, Nepeta rtanjensis and N. argolica subsp. argolica, both characterized by specialized metabolite profiles predominated by iridoids and phenolics, are differentiated according to the stereochemistry of major iridoid aglycone nepetalactone (NL). For the first time, the present study provides a comparative analysis of antimicrobial and immunomodulating activities of the two Nepeta species and their major iridoids isolated from natural sources—cis,trans-NL, trans,cis-NL, and 1,5,9-epideoxyloganic acid (1,5,9-eDLA), as well as of phenolic acid rosmarinic acid (RA). Methanol extracts and pure iridoids displayed excellent antimicrobial activity against eight strains of bacteria and seven strains of fungi. They were especially potent against food-borne pathogens such as L. monocytogenes, E. coli, S. aureus, Penicillium sp., and Aspergillus sp. Targeted iridoids were efficient agents in preventing biofilm formation of resistant P. aeruginosa strain, and they displayed additive antimicrobial interaction. Iridoids are, to a great extent, responsible for the prominent antimicrobial activities of the two Nepeta species, although are probably minor contributors to the moderate immunomodulatory effects. The analyzed iridoids and RA, individually or in mixtures, have the potential to be used in the pharmaceutical industry as potent antimicrobials, and in the food industry to increase the shelf life and safety of food products.

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