scholarly journals Noradrenergic Modulation of Light-Driven Egr-1 Expression in the Adult Visual Cortex

2011 ◽  
Vol 5 ◽  
pp. JEN.S6497 ◽  
Author(s):  
Liisa A. Tremere
Keyword(s):  
Gene Expression ◽  
Visual Cortex ◽  
Neuronal Plasticity ◽  
Adrenergic Receptor ◽  
Receptor Activation ◽  
Early Gene ◽  
Receptor Subtype ◽  
Vertebrate Species ◽  
Regulated Gene Expression ◽  
Noradrenergic Modulation

Noradrenaline has been shown to modulate sensory driven responses in the primary visual cortex (V1) of a number of vertebrate species. Moreover, this neurotransmitter has been postulated to bridge neuronal activation to genomic responses in order to instruct cells in long-lasting changes in neuronal performance. Here we show that local noradrenergic receptor activation in V1 is required for experience-regulated gene expression in the mouse V1. More specifically, we demonstrate that noradrenaline used locally within V1 mediates the light-driven gene expression of egr-1, an immediate early gene implicated as a mediator of neuronal plasticity. Visually-driven egr-1 expression largely depends on the α-adrenergic receptor subtype, with a lesser involvement of the β-subtype. Our findings suggest that noradrenergic transmission regulates plasticity associated gene expression in V1 of awake mice and is well positioned to broadly integrate experience-dependent changes at the cell's membrane and the genomic machinery in neurons.

scholarly journals Genome-Wide Transposon Mutagenesis Identifies a Role for Host Neuroendocrine Stress Hormones in Regulating the Expression of Virulence Genes in Salmonella

2009 ◽  
Vol 192 (3) ◽  
pp. 714-724 ◽  
Author(s):  
H. Spencer ◽  
M. H. Karavolos ◽  
D. M. Bulmer ◽  
P. Aldridge ◽  
S. R. Chhabra ◽  
...  
Keyword(s):  
Gene Expression ◽  
Antimicrobial Peptide ◽  
Receptor Antagonist ◽  
Adrenergic Receptor ◽  
Stress Hormones ◽  
Virulence Gene ◽  
Transposon Mutagenesis ◽  
Environmental Signals ◽  
Virulence Gene Expression ◽  
Regulated Gene Expression

ABSTRACT Bacterial sensing of environmental signals plays a key role in regulating virulence and mediating bacterium-host interactions. The sensing of the neuroendocrine stress hormones epinephrine (adrenaline) and norepinephrine (noradrenaline) plays an important role in modulating bacterial virulence. We used MudJ transposon mutagenesis to globally screen for genes regulated by neuroendocrine stress hormones in Salmonella enterica serovar Typhimurium. We identified eight hormone-regulated genes, including yhaK, iroC, nrdF, accC, yedP, STM3081, and the virulence-related genes virK and mig14. The mammalian α-adrenergic receptor antagonist phentolamine reversed the hormone-mediated effects on yhaK, virK, and mig14 but did not affect the other genes. The β-adrenergic receptor antagonist propranolol had no activity in these assays. The virK and mig14 genes are involved in antimicrobial peptide resistance, and phenotypic screens revealed that exposure to neuroendocrine hormones increased the sensitivity of S. Typhimurium to the antimicrobial peptide LL-37. A virK mutant and a virK mig14 double mutant also displayed increased sensitivity to LL-37. In contrast to enterohemorrhagic Escherichia coli (EHEC), we have found no role for the two-component systems QseBC and QseEF in the adrenergic regulation of any of the identified genes. Furthermore, hormone-regulated gene expression could not be blocked by the QseC inhibitor LED209, suggesting that sensing of hormones is mediated through alternative signaling pathways in S. Typhimurium. This study has identified a role for host-derived neuroendocrine stress hormones in downregulating S. Typhimurium virulence gene expression to the benefit of the host, thus providing further insights into the field of host-pathogen communication.

scholarly journals A dopamine-induced gene expression signature regulates neuronal function and cocaine response

2020 ◽  
Vol 6 (26) ◽  
pp. eaba4221 ◽  
Author(s):  
Katherine E. Savell ◽  
Jennifer J. Tuscher ◽  
Morgan E. Zipperly ◽  
Corey G. Duke ◽  
Robert A. Phillips ◽  
...  
Keyword(s):  
Gene Expression ◽  
Large Scale ◽  
Drugs Of Abuse ◽  
Transcriptional Response ◽  
Gene Expression Signature ◽  
Receptor Activation ◽  
Early Gene ◽  
Behavioral Adaptations

Drugs of abuse elevate dopamine levels in the nucleus accumbens (NAc) and alter transcriptional programs believed to promote long-lasting synaptic and behavioral adaptations. Here, we leveraged single-nucleus RNA-sequencing to generate a comprehensive molecular atlas of cell subtypes in the NAc, defining both sex-specific and cell type–specific responses to acute cocaine experience in a rat model system. Using this transcriptional map, we identified an immediate early gene expression program that is up-regulated following cocaine experience in vivo and dopamine receptor activation in vitro. Multiplexed induction of this gene program with a large-scale CRISPR-dCas9 activation strategy initiated a secondary synapse-centric transcriptional profile, altered striatal physiology in vitro, and enhanced cocaine sensitization in vivo. Together, these results define the transcriptional response to cocaine with cellular precision and demonstrate that drug-responsive gene programs can potentiate both physiological and behavioral adaptations to drugs of abuse.

β-Adrenergic receptor subtype gene expression in timed-pregnant rat myometrium

1997 ◽  
Vol 176 (2) ◽  
pp. 349-357 ◽  
Author(s):  
David Principe ◽  
Mark Sanseverino ◽  
Trevania Saunders ◽  
Mark Phillippe
Keyword(s):  
Gene Expression ◽  
Adrenergic Receptor ◽  
Receptor Subtype ◽  
Pregnant Rat

MAP kinase-dependent induction of clock gene expression by α1 -adrenergic receptor activation

FEBS Letters ◽  
2003 ◽  
Vol 542 (1-3) ◽  
pp. 109-114 ◽  
Author(s):  
Masashi Akiyama ◽  
Yoichi Minami ◽  
Kouji Kuriyama ◽  
Shigenobu Shibata
Keyword(s):  
Gene Expression ◽  
Map Kinase ◽  
Adrenergic Receptor ◽  
Clock Gene ◽  
Receptor Activation ◽  
Clock Gene Expression

scholarly journals Noradrenergic alpha-2A receptor activation suppresses courtship vocalization in male Japanese quail.

2021 ◽  
Author(s):  
Yasuko Tobari ◽  
Ami Masuzawa ◽  
Norika Harada ◽  
Kenta Suzuki ◽  
Simone Meddle
Keyword(s):  
Japanese Quail ◽  
High Frequency ◽  
Adrenergic Receptor ◽  
Receptor Activation ◽  
Early Gene ◽  
Intracerebroventricular Injection ◽  
Receptor Mrna ◽  
Noradrenergic Innervation ◽  
Dose Dependent ◽  
Stimulation Of

Male Japanese quail produce high-frequency crow vocalizations to attract females during the breeding season. The nucleus of intercollicularis (ICo) is the midbrain vocal center in birds and electrical stimulation of the ICo produces calls that include crowing. Noradrenaline plays a significant role in sexual behavior but the contribution of noradrenaline in the control of courtship vocalizations in quail has not been well established. Using dose-dependent intracerebroventricular injection of clonidine, an α2-adrenergic receptor-specific agonist, crowing vocalization was immediately suppressed. At the same time as crow suppression by clonidine there was a reduction of immediate early gene, zenk mRNA, in the ICo; no zenk mRNA expression was detected in the dorsomedial division of the nucleus. Using histochemistry, we determined that the ICo receives noradrenergic innervation and expresses α2A-adrenergic receptor mRNA. Taken together, these data suggest that noradrenaline regulates courtship vocalization in quail, possibly via the alpha2A-adrenergic receptor expressed on ICo neurons.

Distribution of α2-adrenergic receptor subtype gene expression in rat brain

1994 ◽  
Vol 21 (1-2) ◽  
pp. 133-149 ◽  
Author(s):  
Mika Scheinin ◽  
Jon W. Lomasney ◽  
Diane M. Hayden-Hixson ◽  
Uta B. Schambra ◽  
Marc G. Caron ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rat Brain ◽  
Adrenergic Receptor ◽  
Receptor Subtype
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