The Immune Response in Inbred and Outbred Strains of Mice before and after Bone Marrow Transplantation

2013 ◽  
pp. 9
Author(s):  
David Harris ◽  
CL Shultz ◽  
M Badowski ◽  
David Harris
Keyword(s):  
Bone Marrow ◽  
Immune Response ◽  
Bone Marrow Transplantation ◽  
Marrow Transplantation ◽  
Before And After

scholarly journals Cryopreserved ovarian cortex from patients with leukemia in complete remission contains no apparent viable malignant cells

Blood ◽  
2012 ◽  
Vol 120 (22) ◽  
pp. 4311-4316 ◽  
Author(s):  
Tine Greve ◽  
Erik Clasen-Linde ◽  
Morten T. Andersen ◽  
Mette K. Andersen ◽  
Stine D. Sørensen ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Complete Remission ◽  
Quantitative Pcr ◽  
Marrow Transplantation ◽  
Ovarian Tissue ◽  
Malignant Cells ◽  
Ovarian Cortex ◽  
Before And After ◽  
Histology And Immunohistochemistry

Abstract Some women suffering from leukemia require bone marrow transplantation to be cured. Bone marrow transplantation is associated with a high risk of sterility, and some patients are offered fertility preservation by cryopreservation of the ovarian cortex. Transplantation of the ovarian cortex to women cured of leukemia who became menopausal is currently not performed because of the risk of introducing the disease. In this study, individual pieces of ovarian cortex intended for reimplantation from 25 patients with leukemia were transplanted to each of 25 nude mice for 20 weeks. The ovarian cortex was examined before and after transplantation by histology and immunohistochemistry, and RT–quantitative PCR (in the 7 patients with a known marker). Seventeen patients had the ovarian cortex retrieved when they were in complete remission. Before transplantation, 4 of 7 pieces (2 from patients in complete remission) of ovarian cortex had a positive RT–quantitative PCR. After transplantation, none of the mice revealed any sign of disease, neither in the pieces of ovarian cortex transplanted nor in any of the murine organs evaluated. Thus, the ovaries from patients in complete remission do not appear to contain viable malignant cells contrasting ovarian tissue retrieved before treatment.

scholarly journals Phlebotomy to Reduce Iron Overload in Patients Cured of Thalassemia by Bone Marrow Transplantation

Blood ◽  
1997 ◽  
Vol 90 (3) ◽  
pp. 994-998 ◽  
Author(s):  
Emanuele Angelucci ◽  
Pietro Muretto ◽  
Guido Lucarelli ◽  
Marta Ripalti ◽  
Donatella Baronciani ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Iron Overload ◽  
Marrow Transplantation ◽  
Iron Concentration ◽  
Transferrin Saturation ◽  
Dry Weight ◽  
Liver Iron ◽  
Before And After ◽  
Upper Level

In thalassemia after successful bone marrow transplantation (BMT), iron overload remains an important cause of morbidity. After BMT, patients have normal erythropoiesis capable of producing a hyperplastic response to phlebotomy so that this procedure can be contemplated as a method of mobilizing iron from overloaded tissues. A phlebotomy program (6 mL/kg blood withdrawal at 14-day intervals) was proposed to 48 patients with prolonged follow-up (range, 2 to 7 years) after BMT. Seven patients were not submitted to the program (five because of refusal and two because of reversible side effects). The remaining 41 patients (mean age, 16 ± 2.9 years) were treated for a mean period of 35 ± 18 months. All were evaluated before and after 3 ± 0.6 years of followup. Values are expressed as mean ± standard deviation (SD) or as median with a range (25 to 75 percentile). Serum ferritin decreased from 2,587 (2,129 to 4,817) to 417 (210 to 982) μg/L (P < .0001), total transferrin increased from 2.34 ± 0.37 to 2.7 ± 0.58 g/L (P = .0001), transferrin saturation decreased from 90% ± 14% to 50% ± 29% (P < .0001). Liver iron concentration evaluated on liver biopsy specimens decreased from 20.8 (15.5 to 28.1) to 4.2 (1.6 to 14.6) mg/g dry weight (P < .0001). Aspartate transaminase decreased from 2.7 ± 2 to 1.1 ± 0.6 (P < .0001) and alanine transaminase from 5.2 ± 3.4 to 1.7 ± 1.2 (P < .0001) times the upper level of normality. The Knodell score for liver histological activity decreased from 6.9 ± 3 to 4.9 ± 2.8 (P < .0001). These data indicate that phlebotomy is safe, efficient, and widely applicable to ex-thalassemics after BMT.

Exercise Assessment of Cardiac Function in Children and Young Adults Before and After Bone Marrow Transplantation

PEDIATRICS ◽  
1992 ◽  
Vol 89 (4) ◽  
pp. 722-729
Author(s):  
Ranae L. Larsen ◽  
Gerald Barber ◽  
Charles T. Heise ◽  
Charles S. August
Keyword(s):  
Bone Marrow ◽  
Young Adults ◽  
Bone Marrow Transplantation ◽  
Cardiac Function ◽  
Marrow Transplantation ◽  
Cardiac Toxicity ◽  
Intensive Therapy ◽  
Left Ventricular ◽  
Before And After ◽  
Children And Young Adults

Cardiac toxicity is a potential complication of bone marrow transplantation because recipients frequently receive cardiotoxic chemotherapy and/or irradiation before transplantation. Most studies indicate that transient cardiac toxicity occurs within weeks of transplantation, but few studies have evaluated either cardiac status before or late after transplantation. Cardiac performance was assessed via cycle ergometry in 20 children and young adults before transplantation and 31 other children and young adults after transplantation. Mean survival time in the group post-transplantation was 3.9 years with a range of 11 months to 12.1 years. Left ventricular size and shortening fraction at rest were assessed via echocardiography. Data were compared to those of 70 healthy subjects from our laboratory. Patients before and after transplantation had normal oxygen consumptions and cardiac indices at rest. During exercise, however, patients treated for cancer both before and after bone marrow transplantation had reduced exercise times, reduced maximal oxygen consumptions, and reduced ventilatory anaerobic thresholds. Cardiac reserve, as judged by the response of the cardiac output during exercise, was reduced severely. There were no significant differences between the groups tested before and after transplantation. Patients who had been treated for aplastic anemia, who had received less intensive therapy before transplantation, performed significantly better than did patients treated for cancer. Despite these findings, only four patients had abnormalities by echocardiography. In conclusion, before transplantation patients with oncologic diagnoses had serious limitations in exercise performance, most likely as a result of the effects of the cardiotoxic therapy given as part of their conventional cancer therapy. Long-term survivors of bone marrow transplantation also had similar abnormalities. Since the same patients were not studied before and after transplantation, one cannot draw definite conclusions about the effect of the transplantation itself upon cardiac function. However, exercise testing is a sensitive, noninvasive method of assessing patients at risk for cardiac dysfunction secondary to potentially cardiotoxic agents.

Clonal Evolution in Ph-Negative, bcr-Positive Chronic Myeloid Leukaemia before and after Bone Marrow Transplantation

1989 ◽  
Vol 82 (4) ◽  
pp. 205-209 ◽  
Author(s):  
L.M. Secker-Walker ◽  
H.M. Cooke ◽  
P.J. Browett ◽  
J.D. Norton ◽  
C. Kitchen ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Chronic Myeloid Leukaemia ◽  
Myeloid Leukaemia ◽  
Marrow Transplantation ◽  
Clonal Evolution ◽  
Before And After

scholarly journals Phlebotomy to Reduce Iron Overload in Patients Cured of Thalassemia by Bone Marrow Transplantation

Blood ◽  
1997 ◽  
Vol 90 (3) ◽  
pp. 994-998 ◽  
Author(s):  
Emanuele Angelucci ◽  
Pietro Muretto ◽  
Guido Lucarelli ◽  
Marta Ripalti ◽  
Donatella Baronciani ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Iron Overload ◽  
Marrow Transplantation ◽  
Iron Concentration ◽  
Transferrin Saturation ◽  
Dry Weight ◽  
Liver Iron ◽  
Before And After ◽  
Upper Level

Abstract In thalassemia after successful bone marrow transplantation (BMT), iron overload remains an important cause of morbidity. After BMT, patients have normal erythropoiesis capable of producing a hyperplastic response to phlebotomy so that this procedure can be contemplated as a method of mobilizing iron from overloaded tissues. A phlebotomy program (6 mL/kg blood withdrawal at 14-day intervals) was proposed to 48 patients with prolonged follow-up (range, 2 to 7 years) after BMT. Seven patients were not submitted to the program (five because of refusal and two because of reversible side effects). The remaining 41 patients (mean age, 16 ± 2.9 years) were treated for a mean period of 35 ± 18 months. All were evaluated before and after 3 ± 0.6 years of followup. Values are expressed as mean ± standard deviation (SD) or as median with a range (25 to 75 percentile). Serum ferritin decreased from 2,587 (2,129 to 4,817) to 417 (210 to 982) μg/L (P &lt; .0001), total transferrin increased from 2.34 ± 0.37 to 2.7 ± 0.58 g/L (P = .0001), transferrin saturation decreased from 90% ± 14% to 50% ± 29% (P &lt; .0001). Liver iron concentration evaluated on liver biopsy specimens decreased from 20.8 (15.5 to 28.1) to 4.2 (1.6 to 14.6) mg/g dry weight (P &lt; .0001). Aspartate transaminase decreased from 2.7 ± 2 to 1.1 ± 0.6 (P &lt; .0001) and alanine transaminase from 5.2 ± 3.4 to 1.7 ± 1.2 (P &lt; .0001) times the upper level of normality. The Knodell score for liver histological activity decreased from 6.9 ± 3 to 4.9 ± 2.8 (P &lt; .0001). These data indicate that phlebotomy is safe, efficient, and widely applicable to ex-thalassemics after BMT.

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