Tumor Cell Development: A Role for Viruses and Telomerase Activity?

2014 ◽  
Vol 4 ◽  
pp. 7-16 ◽  
Keyword(s):  
Tumor Cell ◽  
Telomerase Activity ◽  
Cell Development

scholarly journals Real-Time RT-PCR Quantification of Human Telomerase Reverse Transcriptase Splice Variants in Tumor Cell Lines and Non–Small Cell Lung Cancer

2007 ◽  
Vol 53 (1) ◽  
pp. 53-61 ◽  
Author(s):  
Eleni Mavrogiannou ◽  
Areti Strati ◽  
Aliki Stathopoulou ◽  
Emily G Tsaroucha ◽  
Loukas Kaklamanis ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Real Time ◽  
Cell Lines ◽  
Splice Variant ◽  
Splice Variants ◽  
Telomerase Activity ◽  
Tumor Cell Lines ◽  
Rt Pcr ◽  
Human Telomerase Reverse Transcriptase ◽  
Tissue Samples

Abstract Background: We developed and validated a real-time reverse transcription (RT)–PCR for the quantification of 4 individual human telomerase reverse transcriptase (TERT) splice variants (α+β+, α−β+, α+β−, α−β−) in tumor cell lines and non–small cell lung cancer (NSCLC). Methods: We used in silico designed primers and a common TaqMan probe for highly specific amplification of each TERT splice variant, PCR transcript–specific DNA external standards as calibrators, and the MCF-7 cell line for the development and validation of the method. We then quantified TERT splice variants in 6 tumor cell lines and telomerase activity and TERT splice variant expression in cancerous and paired noncancerous tissue samples from 28 NSCLC patients. Results: In most tumor cell lines, we observed little variation in the proportion of TERT splice variants. The α+β− splice variant showed the highest expression and α−β+ and α−β− the lowest. Quantification of the 4 TERT splice variants in NSCLC and surrounding nonneoplastic tissues showed the highest expression percentage for the α+β− variant in both NSCLC and adjacent nonneoplastic tissue samples, followed by α+β+, with the α−β+ and α−β− splice variants having the lowest expression. In the NSCLC tumors, the α+β+ variant had higher expression than other splice variants, and its expression correlated with telomerase activity, overall survival, and disease-free survival. Conclusions: Real-time RT-PCR quantification is a specific, sensitive, and rapid method that can elucidate the biological role of TERT splice variants in tumor development and progression. Our results suggest that the expression of the TERT α+β+ splice variant may be an independent negative prognostic factor for NSCLC patients.

The long noncoding RNA HOTAIR activates autophagy by upregulating ATG3 and ATG7 in hepatocellular carcinoma

2016 ◽  
Vol 12 (8) ◽  
pp. 2605-2612 ◽  
Author(s):  
Liang Yang ◽  
Xiuwei Zhang ◽  
Hangyu Li ◽  
Jingang Liu
Keyword(s):  
Hepatocellular Carcinoma ◽  
Tumor Cell ◽  
Long Noncoding Rna ◽  
Antisense Rna ◽  
Noncoding Rna ◽  
Cell Development

The long noncoding RNA HOX transcript antisense RNA (HOTAIR) has been reported to be an oncogene that influences tumor cell development and that correlates with prognosis in hepatocellular carcinoma (HCC).

scholarly journals p53 and FBXW7: Sometimes Two Guardians Are Worse than One

Cancers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 985
Author(s):  
María Galindo-Moreno ◽  
Servando Giráldez ◽  
M. Cristina Limón-Mortés ◽  
Alejandro Belmonte-Fernández ◽  
Carmen Sáez ◽  
...  
Keyword(s):  
Dna Damage ◽  
Tumor Suppressor ◽  
Tumor Cell ◽  
Cell Fate ◽  
Cell Development ◽  
Good Thing ◽  
Cancer Disease

Too much of a good thing can become a bad thing. An example is FBXW7, a well-known tumor suppressor that may also contribute to tumorigenesis. Here, we reflect on the results of three laboratories describing the role of FBXW7 in the degradation of p53 and the possible implications of this finding in tumor cell development. We also speculate about the function of FBXW7 as a key player in the cell fate after DNA damage and how this could be exploited in the treatment of cancer disease.

Effect of modified DNA and RNA oligonucleotides on telomerase activity and tumor cell survival in vitro

2011 ◽  
Vol 47 (8) ◽  
pp. 718-722 ◽  
Author(s):  
L. V. Svinareva ◽  
A. I. Glukhov ◽  
E. Yu. Moskaleva ◽  
V. I. Shvets
Keyword(s):  
Tumor Cell ◽  
Cell Survival ◽  
Telomerase Activity ◽  
Dna And Rna ◽  
Modified Dna ◽  
Tumor Cell Survival

scholarly journals Reciprocal impacts of telomerase activity and tumor cell differentiation in neuroblastoma tumor biology

2021 ◽  
Author(s):  
Eun Young Yu ◽  
Syed S Zahid ◽  
Sarah Aloe ◽  
Erik Falck-Pedersen ◽  
Xi Kathy Zhou ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Tumor Cell ◽  
Tumor Biology ◽  
Telomerase Activity ◽  
Telomere Maintenance ◽  
Growth Potential ◽  
Neuroblastoma Tumor ◽  
Telomere Protein ◽  
Mes Cells ◽  
Tumor Cell Differentiation

Telomere maintenance and tumor cell differentiation have been separately implicated in neuroblastoma malignancy. Their mechanistic connection is unclear. We analyzed neuroblastoma cell lines and morphologic subclones representing the adrenergic (ADRN) and mesenchymal (MES) differentiation states and uncovered sharp differences in their telomere protein and telomerase activity levels. Pharmacologic conversion of ADRN into MES cells elicited consistent and robust changes in the expression of telomere-related proteins. Conversely, stringent down-regulation of telomerase activity triggers the differentiation of ADRN into MES cells, which was reversible upon telomerase up-regulation. Interestingly, the MES differentiation state is associated with elevated levels of innate immunity factors, including key components of the DNA-sensing pathway. Accordingly, MES but not ADRN cells can mount a robust response to viral infections in vitro. A gene expression signature based on telomere and cell lineage-related factors can cluster neuroblastoma tumor samples into predominantly ADRN or MES-like groups, with distinct clinical outcomes. Our findings establish a novel mechanistic connection between telomere and differentiation and suggest that manipulating telomeres may suppress malignancy not only by limiting the tumor growth potential but also by inducing tumor cell differentiation and altering its immunogenicity.

Rules of Tumor Cell Development and Their Application to Biomarkers for Ovarian Cancer

2008 ◽  
Vol 35 (3) ◽  
pp. 403-409 ◽  
Author(s):  
Monica R. McLemore ◽  
Christine Miaskowski ◽  
Bradley E. Aouizerat ◽  
Lee-may Chen ◽  
Marylin Dodd
Keyword(s):  
Ovarian Cancer ◽  
Tumor Cell ◽  
Cell Development
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