scholarly journals Evaluation of Matrix-Assisted Laser Desorption/Ionization Mass spectrometry for second-Generation Lignin Analysis

2012 ◽  
Vol 7 ◽  
pp. ACI.S10799 ◽  
Author(s):  
Aurore Richel ◽  
Caroline Vanderghem ◽  
Mathilde Simon ◽  
Bernard Wathelet ◽  
Michel Paquot
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Size Exclusion ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Molecular Weights ◽  
Desorption Ionization ◽  
Maldi Tof ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Matrix-Assisted Laser Desorption/Ionization time-of-flight (MALDI-TOF) mass spectrometry is evaluated as an elucidation tool for structural features and molecular weights estimation of some extracted herbaceous lignins. Optimization of analysis conditions, using a typical organic matrix, namely α-cyano-4-hydroxycinnamic acid (CHCA), in combination with α-cyclodextrin, allows efficient ionization of poorly soluble lignin materials and suppression of matrix-related ions background. Analysis of low-mass fragments ions ( m/z 100-600) in the positive ion mode offers a “fingerprint” of starting lignins that could be a fine strategy to qualitatively identify principal inter-unit linkages between phenylpropanoid units. The molecular weights of lignins are estimated using size exclusion chromatography and compared to MALDI-TOF-MS profiles. Miscanthus ( Miscanthus x giganteus) and Switchgrass ( Panicum Virgatum L.) lignins, recovered after a formic acid/acetic acid/water process or aqueous ammonia soaking, are selected as benchmarks for this study.

scholarly journals An improved simple method for the identification of Mycobacteria by MALDI-TOF MS (Matrix-Assisted Laser Desorption- Ionization mass spectrometry)

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Adela Alcolea-Medina ◽  
M. T. Cabezas Fernandez ◽  
N. Montiel ◽  
M. P. Luzón García ◽  
C. Delamo Sevilla ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Isolation And Identification ◽  
Simple Method ◽  
Desorption Ionization ◽  
Maldi Tof ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

AbstractThe aim of this study was to establish a simple method for the rapid identification of Mycobacteria species by MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass spectrometry) using the Bruker MALDI-TOF Biotyper system (Bruker Daltonik, Bremen, Germany). A multicentre, prospective, and single blind study was performed in three European Hospitals, two Spanish and one UK hospital from May to August 2018. The BD BACTEC MGIT (Becton Dickinson, Berks, UK) liquid culture system was used in all three centres for the growth of Mycobacteria. When signal positive, tubes were removed from the analyser and in addition to standard laboratory procedures were subcultured on blood agar plates for MALDI-TOF analysis. Plates were incubated aerobically for 1 to 7 days at 37 °C and inspected every day. Once any growth was visible, it was transferred to the steel target plate, overlaid with 1 μl of neat formic acid and 1 μl HCCA matrix (alpha hydroxyl 4 cinnamic acid), and analysed in a Bruker Biotyper MALDI-TOF. Results given by MALDI-TOF were compared with the reference methods used for identification in the different centres. At two Spanish hospitals, identification by MALDI-TOF was only attempted on presumptive non-tuberculosis mycobacteria (NTM) and the results were initially compared with the results obtained by a commercial reverse hybridisation assay, GenoType CM/AS (Hain Lifescience, Tübingen, Germany). At the UK Hospital, identification of any presumptive mycobacteria was attempted and compared with the results obtained by whole genome sequencing (WGS). Overall in 142/167 (85%) of cases the identifications obtained were concordant; all Mycobacterium tuberculosis (MTB) isolates 43/43 (100%), 57/76 (75%) of the rapid growing nontuberculous mycobacteria (NTM), and 42/48 (85%) slow growing NTM tested were identified correctly. We report a new, easy, cheap and quick method for isolation and identification of Mycobacterium spp. without the need for additional steps or equipment and this method is in routine used in all three centres.

scholarly journals Uncatalyzed reactions of 4,4′-diphenylmethane-diisocyanate with polymer polyols as revealed by matrix-assisted laser desorption/ionization mass spectrometry

RSC Advances ◽  
2016 ◽  
Vol 6 (52) ◽  
pp. 47023-47032 ◽  
Author(s):  
Tibor Nagy ◽  
Borbála Antal ◽  
Anita Dékány-Adamoczky ◽  
József Karger-Kocsis ◽  
Miklós Zsuga ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Ionization Mass ◽  
Diphenylmethane Diisocyanate ◽  
Laser Desorption Ionization ◽  
Maldi Tof Mass Spectrometry ◽  
Desorption Ionization ◽  
Maldi Tof ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

The reactivities of various polymer polyols towards MDI were evaluated using MALDI-TOF mass spectrometry.

scholarly journals Effect of Culture Conditions on Microorganism Identification by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

2005 ◽  
Vol 71 (1) ◽  
pp. 58-64 ◽  
Author(s):  
Nancy Valentine ◽  
Sharon Wunschel ◽  
David Wunschel ◽  
Catherine Petersen ◽  
Karen Wahl
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Maldi Tof Ms ◽  
Desorption Ionization ◽  
Maldi Tof ◽  
Tof Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

ABSTRACT Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) has been used to identify bacteria based upon protein signatures. This research shows that while some different proteins are produced by vegetative bacteria when they are cultured in different growth media, positive identification with MALDI-TOF MS is still possible with the protocol established at the Pacific Northwest National Laboratory (K. H. Jarman, S. T. Cebula, A. J. Saenz, C. E. Petersen, N. B. Valentine, M. T. Kingsley, and K. L. Wahl, Anal. Chem. 72:1217-1223, 2000). A core set of small proteins remain constant under at least four different culture media conditions and blood agar plates, including minimal medium M9, rich media, tryptic soy broth (TSB) or Luria-Bertani (LB) broth, and blood agar plates, such that analysis of the intact cells by matrix-assisted laser desorption/ionization mass spectrometry allows for consistent identification.

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