scholarly journals Molecular identification and phylogenetic relationship of Demodex mites based on mitochondrial 16S rDNA

2020 ◽  
Vol 10 (2) ◽  
pp. 136
Author(s):  
AnupKumar Kesavan ◽  
Pawan Prasher ◽  
Dolly Baghra ◽  
Drishtant Singh ◽  
Sharad Thakur ◽  
...  
Keyword(s):  
16S Rdna ◽  
Phylogenetic Relationship ◽  
Molecular Identification ◽  
Mitochondrial 16S Rdna ◽  
Demodex Mites ◽  
Relationship Of

scholarly journals Phylogenetic relationship of Salmonella enterica strains in Tehran, Iran, using 16S rRNA and gyrB gene sequences

2011 ◽  
Vol 5 (06) ◽  
pp. 465-472 ◽  
Author(s):  
Mercedeh Tajbakhsh ◽  
Babak Noory Nayer ◽  
Kamyar Motavaze ◽  
Pedram Kharaziha ◽  
Mohsen Chiani ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rdna ◽  
Phylogenetic Relationship ◽  
Salmonella Enterica ◽  
Phylogenetic Trees ◽  
Gyrb Gene ◽  
Rrna Gene ◽  
Gene Sequences ◽  
Comparison Results ◽  
Relationship Of

Introduction: We assessed whether 16S rDNA and gyrB gene sequences, alone or combined, were suitable for determining the phylogenetic relationship among Salmonella enterica strains isolated from Tehran, Iran.  Patients over five years of age enrolled in an acute diarrheal surveillance project in Tehran province between May 2004 and October 2006 were selected as our study group.  Methodology: 16S ribosomal DNA (rDNA) and gyrB genes from 40 Salmonella isolates obtained from patients with acute diarrhea were sequenced and the data was used to generate phylogenetic trees that facilitated isolate comparison. Results: Salmonella strains clustered into five to seven phylogenetic groups, dependent on analysis of 16S rDNA (1546 bp), gyrB (1256 bp) or a combination of the two genes.  By 16S rDNA sequence analysis, only strains of Salmonella enterica  serovar Typhi ( S. Typhi)  clustered exclusively together.  gyrB sequences permitted clustering of all the S. Typhi and S. Paratyphi A isolates, and clustering of S. Enteritidis into two separate but exclusive groups.  Concatenation of the two data sets did not significantly improve the resolution of the strains compared to the gyrB gene.  None of the analyses completely resolved S. enterica Paratyphi B and C into mutually exclusive groups.   Conclusion: Sequencing of gyrB represents a potentially useful tool for determining the phylogenetic relationship of S. enterica strains in Tehran, Iran. Genetic analysis of the 16S rRNA gene alone or in combination with gyrB did not increase the resolution between serotypes of S. enterica.  We speculate that inclusion of additional genetic markers would improve the sensitivity of the analysis.

scholarly journals Construction of a DNA database for ticks collected in Japan: application of molecular identification based on the mitochondrial 16S rDNA gene

2014 ◽  
Vol 65 (1) ◽  
pp. 13-21 ◽  
Author(s):  
Ai TAKANO ◽  
Hiromi FUJITA ◽  
Teruki KADOSAKA ◽  
Mamoru TAKAHASHI ◽  
Takeo YAMAUCHI ◽  
...  
Keyword(s):  
16S Rdna ◽  
Molecular Identification ◽  
16S Rdna Gene ◽  
Rdna Gene ◽  
Dna Database ◽  
Mitochondrial 16S Rdna

scholarly journals Phylogenetic Relationship of Barnacles (Fam:Balanidae) Sampled on Artificial Substrata of Kuala Selangor and Morib

2019 ◽  
Vol 7 (4.14) ◽  
pp. 82
Author(s):  
N N Roseman ◽  
R Rasol ◽  
A Ismail ◽  
H R Singh ◽  
F Z M Yusof
Keyword(s):  
Phylogenetic Tree ◽  
16S Rdna ◽  
Sequence Comparison ◽  
Morphological Characteristics ◽  
Economic Losses ◽  
Morphological Identification ◽  
Molecular Method ◽  
Artificial Substrata ◽  
Mitochondrial 16S Rdna ◽  
Relationship Of

Barnacles are marine sessile crustacean inhabiting intertidal areas of the Selangor coastline. They are seen attaching themselves to rocks and artificial structures such as jetty, piers, boats and sea walls. Being the most successful biofoulers, barnacles cause economic losses to some extent. Most of barnacles study focused on morphological identification only. Since molecular method gave more accurate results by sequence comparison, species identification was done on samples of obviously different species inhabiting artificial substrata by using mitochondrial 16S rDNA identification. In Kuala Selangor, there was only one species found on artificial substrata in Bagan Pasir and Pasir Penambang which was identified as Amphibalanus cirratus. Two species that differed in their morphological characteristics found on Morib sea walls were identified as Amphibalanus cirratus and Chthamalus malayensis. Phylogenetic tree based on the 16S rDNA showed that all the samples were in the same cluster reflecting that they are in the same clade.  

scholarly journals Phylogenetic relationship of Pasteurella pneumotropica Isolates from Laboratory Rodents Based on 16S rDNA Sequence

2006 ◽  
Vol 68 (6) ◽  
pp. 639-641 ◽  
Author(s):  
Hiraku SASAKI ◽  
Eiichi KAWAMOTO ◽  
Hidehiro UESHIBA ◽  
Hiromi AMAO ◽  
Takuo SAWADA
Keyword(s):  
16S Rdna ◽  
Phylogenetic Relationship ◽  
Rdna Sequence ◽  
16S Rdna Sequence ◽  
Laboratory Rodents ◽  
Relationship Of
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