HPLC-DAD-ESI-MS/MS characterization of bioactive secondary metabolites from Strelitzia nicolai leaf extracts and their antioxidant and anticancer activities In vitro

2018 ◽  
Vol 10 (4) ◽  
pp. 368 ◽  
Author(s):  
Mosad Ghareeb ◽  
Amal Saad ◽  
Wafaa Ahmed ◽  
Laila Refahy ◽  
Sami Nasr
Keyword(s):  
Secondary Metabolites ◽  
Anticancer Activities ◽  
Leaf Extracts ◽  
Esi Ms ◽  
Bioactive Secondary Metabolites

Antibacterial properties in-vitro of Mexican serviceberry extracts against dental biofilm species

2021 ◽  
pp. 1-16
Author(s):  
Erika-Alejandra Salinas-Peña ◽  
Martha Mendoza-Rodríguez ◽  
Claudia Velázquez-González ◽  
Carlo Eduardo Medina-Solis ◽  
América Patricia Pontigo-Loyola ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Antibacterial Properties ◽  
Fusobacterium Nucleatum ◽  
Positive Control ◽  
Dilution Method ◽  
Periodontal Pathogens ◽  
Leaf Extracts ◽  
Dental Biofilm ◽  
Biofilm Bacteria

BACKGROUND: The Mexican serviceberry, Malacomeles denticulata, have been used as a successful oral therapy by Mexican communities without enough scientific support. OBJECTIVE: To evaluate the M. denticulata extracts with selective antibacterial properties over dental biofilm bacteria. METHODS: Fruit, Leaf, and Stem of M. denticulata extracts were evaluated with micro-broth dilution method using ATCC bacteria. OD600 values had compared against each positive control (T-student-test). Anaerobically viability had confirmed by Colony-Forming-Units. Thin-Layer-Chromatography was used to identify the number of compounds and phytochemicals to identify secondary metabolites of the selected extracts. RESULTS: Streptococcus mutans showed Minimum-Bactericidal-Concentrations_(MBC) at 30 mg/mL to Fruit, Leaf, and Stem extracts. Periodontal-pathogens Aggregatibacter actinomycetemcomitans serotype b_(MBC = 30 mg/mL_p <  0.01); Fusobacterium nucleatum subsp. nucleatum_(MBC = 30 mg/mL_p<0.001); Parvimonas micra_(MBC = 15 mg/mL_NS); Porphyromonas gingivalis_(MBC = 30 mg/mL_NS); and Prevotella intermedia_(MBC = 3.75 mg/mL_NS) presented higher sensitivity to Leaf-Methanol, than the primary colonizers. Phytochemicals showed positive results to anthraquinones, coumarins, flavonoids, saponins, saponins steroids/triterpenoids, steroids/triterpenes, and tannins/phenols. CONCLUSION: We suggest the natural extracts of fruit and leaf of the Mexican serviceberry for the preventive use over the oral cariogenic or periodontal biofilm species, by their selective antibacterial properties against pathogenic species evaluated in-vitro, and due to the presence of antibacterial secondary metabolites identified as flavonoids and saponins of M. denticulata leaf extracts.

scholarly journals Cytokinin Mediated Increased In Vitro Production of Secondary Metabolites with Special Reference to Solasodine in Solanum erianthum

2021 ◽  
Vol 8 (02) ◽  
pp. e62-e68
Author(s):  
Jeeta Sarkar ◽  
Nirmalya Banerjee
Keyword(s):  
Secondary Metabolites ◽  
High Performance ◽  
Culture Medium ◽  
In Vitro Production ◽  
Leaf Extracts ◽  
Plant Parts ◽  
Regenerated Plants ◽  
Vitro Production ◽  
Regenerated Plantlets

AbstractSteroid alkaloid solasodine is a nitrogen analogue of diosgenin and has great importance in the production of steroidal medicines. Solanum erianthum D. Don (Solanaceae) is a good source of solasodine. The aim of this study was to evaluate the effect of different cytokinins on the production of secondary metabolites, especially solasodine in the in vitro culture of S. erianthum. For solasodine estimation, field-grown plant parts and in vitro tissues were extracted thrice and subjected to high-performance liquid Chromatography. Quantitative analysis of different secondary metabolites showed that the amount was higher in the in vitro regenerated plantlets compared to callus and field-grown plants. The present study critically evaluates the effect of the type of cytokinin used in the culture medium on solasodine accumulation in regenerated plants. The highest solasodine content (46.78±3.23 mg g-1) was recorded in leaf extracts of the in vitro grown plantlets in the presence of 6-γ,γ-dimethylallylamino purine in the culture medium and the content was 3.8-fold higher compared to the mother plant.

Synthesis and characterization of novel PUFA esters exhibiting potential anticancer activities: An in vitro study

2011 ◽  
Vol 46 (10) ◽  
pp. 4878-4886 ◽  
Author(s):  
Azmat Ali Khan ◽  
Mahboob Alam ◽  
Saba Tufail ◽  
Jamal Mustafa ◽  
Mohammad Owais
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Synthesis And Characterization ◽  
Anticancer Activities

scholarly journals Synthesis and Characterization of 2-Pyrazoline Derivatives and their in silico and in vitro Studies on Antimicrobial and Anticancer Activities

2019 ◽  
Vol 31 (10) ◽  
pp. 2191-2196 ◽  
Author(s):  
S. Rathinamanivannan ◽  
K. Megha ◽  
Raja Chinnamanayakar ◽  
Ashok Kumar ◽  
M.R. Ezhilarasi
Keyword(s):  
Breast Cancer ◽  
Synthesis And Characterization ◽  
Anticancer Activities ◽  
1H And 13C Nmr ◽  
Chemical Structures ◽  
Ft Ir ◽  
Affinity Score ◽  
High Binding Affinity

The new series of 1-(4,5-dihydro-5-phenyl-3-diphenylpyrazol-1-yl)butan-1-one derivatives were synthesized by cyclization method using biphenyl chalcone with n-butyric acid and hydrazine hydrate. The synthesized 1-(4,5-dihydro-5-phenyl-3-diphenylpyrazol-1-yl)butan-1-one derivatives chemical structures were confirmed from spectral data such as FT-IR, 1H and 13C NMR. 2-Pyrazoline derivatives were docked with bacterial (1UAG) and breast cancer (1OQA) protein. Based on high binding affinity score, the best compound was subjected to in vitro anticancer activity by MTT assay. Also, antimicrobial activity were studied for synthesized 2-pyrazoline derivatives.

Synthesis and characterization of two novel dicyanidoargentate(i) complexes containing N-(2-hydroxyethyl)ethylenediamine exhibiting significant biological activity

2014 ◽  
Vol 38 (10) ◽  
pp. 4760-4773 ◽  
Author(s):  
Nesrin Korkmaz ◽  
Ahmet Karadağ ◽  
Ali Aydın ◽  
Yusuf Yanar ◽  
İsa Karaman ◽  
...  
Keyword(s):  
Biological Activity ◽  
Synthesis And Characterization ◽  
Anticancer Activities

Two novel cyanido-complexes having very significant antibacterial, antifungal and anticancer activities in vitro were synthesized and characterized using various techniques.

Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativumL.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities

2016 ◽  
Vol 68 (6) ◽  
pp. 988-1000 ◽  
Author(s):  
Nemanja S. Stanisavljević ◽  
Marija D. Ilić ◽  
Ivana Z. Matić ◽  
Živko S. Jovanović ◽  
Tihomir Čupić ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Anticancer Activities ◽  
Seed Coats

scholarly journals Secondary metabolites of Phlebopus species from Northern Thailand

2020 ◽  
Vol 19 (12) ◽  
pp. 1525-1536
Author(s):  
Boontiya Chuankid ◽  
Hedda Schrey ◽  
Benjarong Thongbai ◽  
Olivier Raspé ◽  
Norbert Arnold ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
2D Nmr ◽  
Cytotoxic Activities ◽  
Tumor Cell Lines ◽  
Submerged Cultures ◽  
Esi Ms ◽  
Spectroscopic Analyses ◽  
New Compounds ◽  
Comprehensive Characterization

AbstractSubmerged cultures of the edible mushrooms Phlebopus portentosus and Phlebopus spongiosus were screened for their secondary metabolites by HPLC-UV/Vis and HR-LC-ESI-MS. Two new compounds, 9′-hydroxyphenyl pulvinone (1), containing an unusual pulvinone structure, and phlebopyron (2), together with the seven known pigments, atromentic acid (3), xerocomic acid (4), variegatic acid (5), methyl atromentate (6), methyl isoxerocomate (7), methyl variegatate (8), and variegatorubin (9) were isolated from the cultures. Their structures were assigned on the basis of extensive 1D/2D NMR spectroscopic analyses, as well as HR-ESI-MS, and HR-ESI-MS/MS measurements. Furthermore, the isolated compounds were evaluated for their antimicrobial and cytotoxic properties. 9′-hydroxyphenyl pulvinone (1), xerocomic acid (4), and methyl variegatate (8) exhibited weak to moderate cytotoxic activities against several tumor cell lines. The present paper provides a comprehensive characterization of pigments from the class of pulvinic acids that are present in the basidiomes of many edible bolete species.

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