scholarly journals In vitro antiproliferative effect of earthworm coelomic fluid of Eudrilus eugeniae, Eisenia foetida, and Perionyx excavatus on squamous cell carcinoma-9 cell line: A pilot study

2017 ◽  
Vol 9 (5) ◽  
pp. 61 ◽  
Author(s):  
Dominic Augustine ◽  
RoopaS Rao ◽  
Jayaraman Anbu ◽  
KN Chidambara Murthy
Keyword(s):  
Squamous Cell Carcinoma ◽  
Pilot Study ◽  
Cell Carcinoma ◽  
Cell Line ◽  
Squamous Cell ◽  
Coelomic Fluid ◽  
Eisenia Foetida ◽  
Eudrilus Eugeniae ◽  
Perionyx Excavatus

scholarly journals Establishment of a Tongue Squamous Cell Carcinoma Cell Line from Indian Gutka Chewer

2014 ◽  
Vol 2014 ◽  
pp. 1-9
Author(s):  
Tejas T. Patil ◽  
Pradnya K. Kowtal ◽  
Abhijeet Nikam ◽  
Madan S. Barkume ◽  
Asawari Patil ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Retinoic Acid ◽  
Cell Carcinoma ◽  
Cell Line ◽  
Squamous Cell ◽  
Scid Mice ◽  
Oral Cancers ◽  
Hpv Status ◽  
Epithelial Phenotype

CD cell line has been established from a poorly differentiated squamous cell carcinoma of tongue. This is a first ever cell line established from an Indian gutka chewer. Cell line was characterized for morphology, ultrastructure, doubling time, expression of epithelial markers, DNA content, karyotyping, STR markers, p53 mutations, HPV status, and tumorigenicity in SCID mice with all-trans-retinoic acid and cisplatin. The epithelial phenotype of the cell line was confirmed with surface markers and ultrastructure. The cell line is hyperploid with chromosomal alterations like gain of chromosomes 8q and 11q. CD cell line shows a unique pattern on STR genotyping and carries a missense mutation R273C in TP53. It does not show genomic integration of HPV. The cells are nontumorigenic to SCID mice and show growth inhibition upon treatment with cisplatin, and all-trans-retinoic acid. This cell line may be useful as an in vitro tool to understand the molecular changes associated with oral cancers.

scholarly journals Tongue Cancer and Epigenetic Factors: An in-vitro Study on 298 Micro-RNAS

2012 ◽  
Vol 10 (3) ◽  
pp. 447-454
Author(s):  
A. Guida ◽  
G. Pannone ◽  
A. Lucchese ◽  
R. Serpico ◽  
D. Pasquali ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Line ◽  
Cell Lines ◽  
Squamous Cell ◽  
Tongue Cancer ◽  
In Vitro Study ◽  
Early Results ◽  
Micro Rnas

Oral Squamous Cell Carcinoma (OSCC) is the sixth most frequent malignant tumour. There is some evidence that tongue cancer has a higher local failure rate and poorer prognosis than other anatomical sites in the oral cavity. We used tongue squamous cell carcinoma cell lines harbouring mutated p53/p16 as tongue cancer models to study the influences exerted by p53 and p16 genes on the expression of micro RNAs (miRNAs). The study was performed on microarray chips harbouring 298 miRNA sequences. OSCC cell lines used in this study were SCC-4, SCC-15 and SCC-25, all three carrying mutated/hypermethylated p53/p16. The expression values normalized to healthy control of 298 miRNAs were obtained for each cell line. MiRNA 196b was found hyperexpressed in the three cell lines. MiRNAs 19b-1, 21, 27a, 30d, 134, 339, 379 and 465 were found altered in two out of three cell lines. miRNAs found altered in one cell line out of three were: 7b, 23a, 25, 30c, 30e-3p, 107,125b, 124a, 214, 216, 325 and 384. A literature review for each miRNA found significant was undertaken. Some miRNAs have a well-known role in oral cancer, some have been put in correlation with other cancers/diseases, others are found significant for the first time. These early results in tongue cancer cell lines harbouring mutation of p16/p53 need further analyses to understand whether this variation of miRNA levels are directly influenced by the malfunction of these proteins or if, vice-versa, altered miRNA levels influence the function of p16 and p53.

scholarly journals Cytotoxic Effects of Different Aromatic Plants Essential Oils on Oral Squamous Cell Carcinoma- an in vitro Study

2019 ◽  
Vol 23 (2) ◽  
pp. 73-79
Author(s):  
Miloš Lazarević ◽  
Maja Milošević ◽  
Nada Petrović ◽  
Slobodan Petrović ◽  
Giuseppe Damante ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Essential Oils ◽  
Cell Carcinoma ◽  
Cell Line ◽  
Squamous Cell ◽  
Mentha Piperita ◽  
Proliferative Effect ◽  
Viable Cells

Summary Background/Aim: Current approaches in therapy of head and neck cancers are surgery, radiotherapy and chemotherapy. However, recurrence, development of multidrug resistance, side effects, and high costs of therapy are significant problems which point to the need for more efficient and less toxic drugs and interventions. Material and Methods: Eight essential oils obtained from Thymus serpyllum, Mentha piperita, Juniperus communis, Rosmarinus officinalis, Melissa officinalis, Achillea millefolium, Zingiber officinale, and Helichrysum arenarium were tested for their anti-proliferative on oral squamous cell carcinoma (OSCC) culture and SCC-25 cell line. Cytotoxicity assays (MTT and Neutral red) were used to detect the effect of the mentioned essential oils. Results: T. serpyllum, M. piperita, J. communis, and R. officinalis essential oils exhibited the best anti-proliferative effect, on both types of cells. M. piperita had the greatest effect on SCC-25 cell line (4,5% of viable cells) and OSCC cells (7,2% of viable cells). Overall, cytotoxicity was higher in OSCC than in SCC-25 cell line. Conclusions: This study showed a clear anti-proliferative effect of four essential oils, in vitro making them novel potential antineoplastic agents.

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