Phytochemical screening, antibacterial-guided fractionation, and thin-layer chromatographic pattern of the extract obtained from Diploknema butyracea

2020 ◽  
Vol 12 (4) ◽  
pp. 437
Author(s):  
Ravin Bhandari ◽  
Supriya Tiwari ◽  
Samjhana Nepal ◽  
Shraddha Sigdel ◽  
Sarju Bhattarai ◽  
...  
Keyword(s):  
Thin Layer ◽  
Phytochemical Screening ◽  
Chromatographic Pattern

scholarly journals PHYTOCHEMICAL INVESTIGATION OF THE MEDICINAL PLANT MORICANDIA ARVENSIS L. FROM ALGERIAN SAHARA

2018 ◽  
Vol 11 (5) ◽  
pp. 450 ◽  
Author(s):  
Abdelaziz Berreghioua ◽  
Abdelkrim Cheriti
Keyword(s):  
Medicinal Plant ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Acetone Extract ◽  
Phytochemical Screening ◽  
Algerian Sahara ◽  
Phytochemical Investigation ◽  
Moricandia Arvensis ◽  
Chemical Groups ◽  
Layer Chromatography

Objective: The aim of this research was to isolate and identify flavonoids extracted from the leaves of Moricandia arvensis.Methods: The phytochemical screening reaction and thin-layer chromatography have been used to characterize the chemical groups, before they were identified by nuclear magnetic resonance.Results: The leaves contain essentially flavonoids, tannins, cardenolides, saponins, and alkaloids. The phytochemical investigation of the water-acetone extract led to the isolation of five flavonoids derivatives, namely: 5,7-dihydroxy-3,6,4’-trimethoxyflavone (1); 5,7,4’-trihydroxy- 3,6,8,3’-tetramethoxyflavone (2); 3,3’,4’, 5,7- pentahydroxy flavanone (3); 3-glucosyl 3’,4’,5,7 tetrahydroxy flavonol (4); and kaempférol-3- digalactopyranoside (5). The structures of 1–5 were identified by comparison of their spectral data with those reported in the literature.Conclusion: In this work, it was possible to isolate and identify five flavonoids after fractionation of the hydroacetone extract from the leaves of the medicinal plant M. arvensis.

scholarly journals PHARMACOGNOSTIC SCREENING STUDIES OF JUSTICIA GENDARUSSA BURM. LEAVES FOUND IN DEHRADUN DISTRICT OF UTTARAKHAND

2017 ◽  
Vol 10 (16) ◽  
pp. 83
Author(s):  
Nondita Prasad ◽  
Balbir Singh ◽  
Diksha Puri
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Large Range ◽  
Quantitative Estimation ◽  
Biological Activities ◽  
Water Soluble ◽  
Phytochemical Screening ◽  
Geographical Differences ◽  
Layer Chromatography ◽  
Chloroform Methanol

  Objective: Justicia gendarussa Burm. (family Acanthaceae) commonly known as nilinirgundi, is found in Southern India possesses multifarious biological activities due to large range of phytoconstituents. The present study is designed to evaluate the various pharmacognostic parameters of the leaves of J. gendarussa, found in Dehradun district of Uttarakhand for its authentication.Methods: Fresh leaves were taken for the morphological and microscopical (histology and powder) evaluation. Physicochemical parameters (ash values, extractives values, florescence analysis, microbial contamination, and loss on drying) were also performed. Phytochemical screening and thin-layer chromatographic fingerprinting of extracts were also performed to check the presence of various phytoconstituents.Results: The microscopy of the leaves evinced the presence of anisocytic stomata, cuboidal calcium oxalate crystals, cystoliths, multicellular covering trichomes, starch grains and oil globules. The quantitative estimation of total ash, acid insoluble, and water soluble ash values were 13.8%, 1.2%, and 4.5% w/w, respectively. The alcohol soluble and water soluble extractives were estimated as 11.45% and 15.67% w/w, respectively. Foreign organic matter and loss on drying values obtained were 0.23% and 11.2% w/w. Phytochemical screening of petroleum ether, chloroform, methanol and aqueous extracts ascertained the presence of alkaloids, phenolic compounds, saponins, tannins, carbohydrates, flavonoids, glycosides, steroids and triterpenoids. The thin-layer chromatography (TLC) profiling of different extracts revealed the presence of potential compounds which can be further isolated with the help of high-performance liquid chromatography or high-performance TLC.Conclusion: The results of this study provide suitable standards for the authentication of this plant. In the present study, there are certain variations observed from the evaluations done on the same species by other research groups. The probable reason suggested for such disparity is due to the environmental and geographical differences in the locations of the plant collected.

scholarly journals PHARMACOGNOSTIC STANDARDIZATION, PRELIMINARY PHYTOCHEMICAL SCREENING AND TLC FINGERPRINTING OF THE BARK OF CASCABELA THEVETIA L.

2019 ◽  
pp. 125-130
Author(s):  
Neelutpal Gogoi ◽  
Biman Bhuyan ◽  
Trinayan Deka
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Thin Layer Chromatography ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Alcoholic Extract ◽  
Phytochemical Screening ◽  
Cork Cell ◽  
Layer Chromatography

Objectives: In this study, systematic pharmacognostic study and preliminary phytochemical screening of the bark of Cascabela thevetia L. were carried out. Methods: The selected plant part was collected, processed and stored in an airtight container. From the bark different pharmacognostic studies like macroscopic and microscopic evaluation, physicochemical parameters, fluorescence analysis were done. Powdered bark was successively extracted by petroleum ether, chloroform, ethyl acetate, and methanol using a Soxhlet apparatus and finally macerated with the hydro-alcoholic solvent system (30:70). The preliminary phytochemical analysis and thin layer chromatography of the extracts were done to find the nature and number of the different phytoconstituents present. Results: Transverse microscopy reveals the presence of crystal oxalate, cork cell, starch granules, vascular bundle, phloem fiber, parenchyma cells, and collenchyma cells. Powder microscopy also showed the presence of cork cell, fiber and calcium oxalate crystal. Results obtained in different physicochemical analysis like total ash, acid insoluble ash, water soluble ash, alcohol-soluble extractive, water-soluble extractive, and moisture content were 8.67%, 0.83%, 5.33%, 4.53%, 12.27%, and 7.83% respectively. Phytochemical analysis showed the presence of alkaloid, flavonoid, triterpenoid, phytosterol, tannin, saponin, anthraquinone, carbohydrate and fatty acid in the different extracts. TLC (Thin Layer Chromatography) study revealed 4 spots in petroleum ether, chloroform, ethyl acetate, and methanol extracts and 3 spots in the Hydro-alcoholic extract with different solvent systems. Conclusion: The results obtained from the study will provide a reliable basis for identification, purity, and quality of the plant.

scholarly journals Pharmacognostical, Physicochemical and Preliminary Phytochemical Standardization of Aerial Parts of Onosma bracteatum Wall

2017 ◽  
Vol 9 (6) ◽  
Author(s):  
Patel V. G. ◽  
Patel K. G. ◽  
Patel K. V. ◽  
Gandhi T. R.
Keyword(s):  
Phenolic Compounds ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Microscopical Examination ◽  
Phytochemical Screening ◽  
Aerial Parts ◽  
Inorganic Acids ◽  
Physicochemical Evaluation ◽  
Thin Walled ◽  
Layer Chromatography

Objective: The present study was undertaken to develop the standardization parameters of powdered aerial parts of Onosma bracteatum Wall, family Boraginaceae. Methods: Different parameters such as pharmacognostical, physicochemical, preliminary phytochemical evaluation along with thin layer chromatography for identification of phytoconstituents were studied. Results: On microscopical examination of the aerial parts it showed the presence of oval to polygonal thin walled straight epidermal cells; spiral vessels, a few fibres elongated with blunt tips, long warty, tubercle based unicellular hairs and paracytic stomata. On physicochemical evaluation it was found to contain more amount of polar constituents as the ethanol extractive value was found to be more. Total ash value and acid insoluble ash indicated the presence of inorganic acids and silicaceous matter respectively. Foaming index and swelling index were indicative of saponins and mucilaginous matter present in the aerial parts. On preliminary phytochemical screening and thin layer chromatographic studies it revealed the presence of saponins, flavonoids, phenolic compounds and mucilage. Conclusion: The present work carried out can serve as a purpose for identification, authentication and standardization of the crude drug.

scholarly journals HEPATOPROTECTIVE ACTIVITY OF THE WHOLE PLANT OF NEPTUNIA PROSTRATA L. IN CARBON TETRACHLORIDE INDUCED RATS

2021 ◽  
pp. 56-59
Author(s):  
HONEY JAJO ◽  
RAJAT GHOSH
Keyword(s):  
Thin Layer ◽  
Petroleum Ether ◽  
Thin Layer Chromatography ◽  
Methanolic Extract ◽  
Hepatoprotective Activity ◽  
Phytochemical Screening ◽  
Standard Drug ◽  
Whole Plant ◽  
Layer Chromatography ◽  
Serum Glutamate

Objective: The aim of this study is to investigate the hepatoprotective activity of the whole plant of Neptunia Prostrata L. Methods: The whole plant was collected and identified as Neptunia Prostrata L. The collected plants were shade dried and pulverized to fine powdered of particle size (#) 40. It was then defatted with petroleum ether for 24 hour and soaked with methanol and ethanol, respectively. The extracts was filtered and distilled off using a rotary evaporator. The phytochemical screening of the extracts was carried out and thin layer chromatography study was also done. Acute toxicity study and in vivo hepatoprotective activity of the methanolic extract using CCL4 (carbon tetra chloride) induced model was investigated. Results: The phytochemical screening revealed the presence of alkaloids, glycosides (saponins), flavonoids, tannins, carbohydrates, proteins, phenolic, steroids and terpenoids. Thin-layer chromatography of the methanolic and ethanolic extracts with their fractions using different solvents were performed by taking petroleum ether and ethyl acetate (2:8) as mobile phase system and were able to observe the presence of many spots. Oral administration of methanolic extract of Neptunia prostrata at doses till 2000 mg/kg was found safe and shows good hepatoprotective activity by showing decreased levels of serum SGOT (serum glutamate oxaloacetate transaminase), SGPT (serum glutamate pyruvate transaminase) and ALP (alkaline phosphatase) when compared with the standard drug silymarin. Conclusion: The preliminary phytochemical screening of the methanol and ethanolic extract shows phytoconstituents such as flavonoids, triterpenoids, tannins, saponins, alkaloids and chromatographic studies indicates the presence of several components in varying abundance. The decrease of serum bilirubin level by the methanolic extract of the plant shows hepatoprotective activity. It has confirmed the traditional claim for its use in the treatment of jaundice.

scholarly journals Identifikasi Senyawa Metabolit Sekunder pada Durian (Durio zibethinus Murr)

2019 ◽  
Vol 1 (1) ◽  
pp. 1-6
Author(s):  
Aji Suteja ◽  
Emmy Harso Kardhinata ◽  
Rosliana Lubis
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Experimental Method ◽  
Thin Layer Chromatography ◽  
Secondary Metabolite ◽  
Phytochemical Screening ◽  
Screening Analysis ◽  
Durio Zibethinus ◽  
Rf Values ◽  
Layer Chromatography

This study aims to determine how to identify the types of secondary metabolite compounds contained in durian leaves. The study was conducted by an experimental method by identifying secondary metabolite compounds on durian leaves using phytochemical screening. The sample criteria used were the leaf buds and all the leaf strands (except the leaf bones). The results of the study showed that of the three types of durian leaves namely copper, baskets and Sp A. There are several types of secondary metabolite compounds including alkaloids, steroids, and terpenoids. Phytochemical screening analysis was carried out using thin layer chromatography by showing RF values on three types of durian leaves using methanol and ethyl acetate solvents in a ratio of 3: 1. RF value on copper durian is 0.97, bakul durian is 0.95 and Sp durian. A 0.94.

scholarly journals Preliminary Phytochemical Screening and Thin Layer Chromatography Analysis of Stem Bark Extracts of African Mistletoe Parasitic on Vitellaria paradoxa, Piliostigma thonningii and Combretum fragrans

2019 ◽  
pp. 1-6
Author(s):  
T. Agber Cyprian ◽  
Shaakaa Sewuese ◽  
Linus U. Akacha
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Heart Diseases ◽  
Stem Bark ◽  
Phytochemical Screening ◽  
Vitellaria Paradoxa ◽  
Chromatography Analysis ◽  
Plant Hosts ◽  
Layer Chromatography

Aim: Mistletoes are highly utilized in traditional medicine to treat different kinds of diseases such as heart diseases, diabetes and malaria, among others. The chemistry of African mistletoe is not sufficiently documented. This paper is therefore, aimed at determining the phytochemicals present in the crude extracts of mistletoe parasitic on plants that are commonly seen as hosts. Study Design: This study was designed to compare the phytochemical profiles of mistletoe stem barks obtained from different plant hosts. Place and Duration of Study: Department of Chemistry, University of Agriculture, Makurdi, Benue State Nigeria, between August and September, 2018.   Methodology: Powdered stem bark of mistletoe was extracted successively with hexane, ethyl acetate and methanol. Preliminary phytochemical screening was carried out on the extracts. Thin layer chromatography (TLC) was carried out on silica gel precoated plates in 9:1 (hexane/ethyl acetate), 1:1 (hexane/ethyl acetate), and 7:3 (ethyl acetate/methanol) mobile phases for hexane, ethyl acetate and methanol extracts respectively. Results: The study revealed the presence of secondary metabolites such as alkaloids, flavonoids, tannins/phenols, cardiac glycosides, steroids and triterpenoids. It was evident from TLC analysis that mistletoes from various plant hosts contain similar chemical profile. Conclusion: We therefore debunk the claim by some herbalists that medicinal values of mistletoes vary due to host plant. This is the first time a study of this kind is reported on mistletoe parasitic on Vitellaria paradoxa Pilostigma thonningii, Combretum fragrans.

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