scholarly journals Angiotensin-converting enzyme 2 polymorphism and COVID-19: Molecular change and natural history of illness in different settings

2021 ◽  
Vol 0 (0) ◽  
pp. 0
Author(s):  
Rujittika Mungmunpuntipantip ◽  
Viroj Wiwanitkit
Keyword(s):  
Natural History ◽  
Angiotensin Converting Enzyme ◽  
Converting Enzyme ◽  
Angiotensin Converting Enzyme 2 ◽  
Molecular Change ◽  
History Of

scholarly journals Effect of COVID-19 infection on characteristics of sperm in men with impaired fertility

2021 ◽  
Vol 22 (3) ◽  
pp. 25-33
Author(s):  
T. M. Sorokina ◽  
E. E. Bragina ◽  
E. A. Sorokina ◽  
A. O. Sedova ◽  
M. I. Shtaut ◽  
...  
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Sperm Quality ◽  
Sperm Count ◽  
World Health ◽  
Research Centre ◽  
Control Group ◽  
Converting Enzyme ◽  
Angiotensin Converting Enzyme 2 ◽  
Sperm Analysis ◽  
History Of

Introduction. The risk of infection with SARS-CoV-2 virus and potential cross-infection in an in vitro fertilization laboratory remains unclear. SARS-CoV-2 enters the body through angiotensin-converting enzyme 2 receptor. There is a possibility that high expression of angiotensin-converting enzyme 2 on testicular cells can lead to spermatogenesis disfunction and decreased quality of the ejaculate.The study objective is to compare quantitative and qualitative sperm characteristics in patients who sought medical help at the Laboratory of Genetics of Reproductive Disorders at the Research Centre for Medical Genetics with reproduction problems between 2017 and 2020 with a focus on the period of COVID-19 pandemic.Materials and methods. Results of semen analysis of 4403 patients (mean age 34 years) were considered. Taking into account histories of patients examined in 2020, a separate group of 428 men was formed with 42 men with a history of coronavirus infection confirmed by lab tests (40 cases of mild disease, 2 cases of moderate disease). In 14 patients, sperm analysis was performed twice: prior to COVID-19 and after. Standard sperm analysis was performed in accordance with the World Health Organization Guidelines. For statistical analysis Student’s t-test was used with significance level p <0.05.Results. Results of 2020 semen analyses were compared to the control group including 3300 patients examined between 2017 and 2019 prior to emergence of COVID-19 virus in Russia. Decreased mean sperm count was observed in patients examined in 2020 compared to the control group: 302 ± 6 million versus 250 ± 8 million with concurrent increased sperm motility (14.7 ± 0.2 % versus 16.5 ± 0.4 %) and improved sperm morphological (3.07 ± 0.05 % versus 3.92 ± 0.11 %). The differences for these three sperm parametres are statistically significant. In 2020, normospermia was observed in 10.3 % of cases which does not significantly differ from 2017 and 2019 levels. Comparison of semen analyses in 2020 showed that the percentage of normospermia in patients after COVID-19 infection was almost 2-fold lower than in the control group of patients without history of the infection (7.1 % vs 13 %) and percentage of azpoospermia was more 2.5-fold higher (7.1 % vs 2.5 %). In 14 patients, sperm analyses before and after COVID-19 were compared. Mean sperm count after the disease decreased from 244 ± 74 million to 178 ± 34 million. Mean motility characteristics (12.7 and 15.8 %) and normal sperm morphology (2.6 and 3.2 %), on the other hand, had an upward trend after the disease. However, these differences were not statistically significant.Conclusion. In conclusion, SARS-CoV-2 virus and condition of COVID-19 pandemic did not lead to serious decrease in sperm quality in the studied group of Russian patients in 2020. This can be associated with small number of patients after the infection included in the sample, as well as general trend toward improvement in sperm quality observed in recent years.

Thermodynamics of the interaction between the spike protein of severe acute respiratory syndrome- coronavirus-2 and the receptor of human angiotensin converting enzyme 2. Effects of possible ligands

2020 ◽  
Author(s):  
Cristina Garcia-Iriepa ◽  
Cecilia Hognon ◽  
Antonio Francés-Monerris ◽  
Isabel Iriepa ◽  
Tom Miclot ◽  
...  
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Molecular Mechanisms ◽  
Molecular Design ◽  
Binding Free Energy ◽  
Transmembrane Protein ◽  
Spike Protein ◽  
Converting Enzyme ◽  
Angiotensin Converting Enzyme 2 ◽  
Rational Molecular Design ◽  
Rational Evaluation

<div><p>Since the end of 2019, the coronavirus SARS-CoV-2 has caused more than 180,000 deaths all over the world, still lacking a medical treatment despite the concerns of the whole scientific community. Human Angiotensin-Converting Enzyme 2 (ACE2) was recently recognized as the transmembrane protein serving as SARS-CoV-2 entry point into cells, thus constituting the first biomolecular event leading to COVID-19 disease. Here, by means of a state-of-the-art computational approach, we propose a rational evaluation of the molecular mechanisms behind the formation of the complex and of the effects of possible ligands. Moreover, binding free energy between ACE2 and the active Receptor Binding Domain (RBD) of the SARS-CoV-2 spike protein is evaluated quantitatively, assessing the molecular mechanisms at the basis of the recognition and the ligand-induced decreased affinity. These results boost the knowledge on the molecular grounds of the SARS-CoV-2 infection and allow to suggest rationales useful for the subsequent rational molecular design to treat severe COVID-19 cases.</p></div>

Sign in / Sign up

Export Citation Format

Share Document