scholarly journals Evaluation and comparison of Vitamin D receptors in periodontal ligament tissue of Vitamin D-deficient chronic periodontitis patients before and after supplementation of Vitamin D3

2019 ◽  
Vol 23 (2) ◽  
pp. 100 ◽  
Author(s):  
VishakhaSarang Patil ◽  
RohiniSalil Mali ◽  
AlpanaSubodh Moghe
Keyword(s):  
Vitamin D ◽  
Vitamin D3 ◽  
Chronic Periodontitis ◽  
Periodontal Ligament ◽  
Vitamin D Receptors ◽  
Before And After

SUBLINGUAL VITAMIN D3 DRUG THERAPY IN VITAMIN D DEFICIENCY PATIENTS AT PRAVARA RURAL HOSPITAL

2019 ◽  
pp. 18-20
Author(s):  
Sanjeeva Kumar Goud T ◽  
Rahul Kunkulol
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Vitamin D3 ◽  
Serum Vitamin ◽  
Cross Sectional ◽  
Serum Vitamin D ◽  
Significant Difference ◽  
Before And After

The present study was aimed to study the effect of Sublingual Vitamin D3 on Serum Vitamin D level in Vitamin D deficiency patients. This was a cross-sectional and interventional study. All the Vitamin D deficiency patients of age 18-60years and either gender, willing to participate in the study were included. Patients who had greater than 20 ng/ml were excluded from the study. The total number of participants in our study was 200, out of these 111 males and 89 females, the mean age in our study was 51.07 ± 7.39Yrs. All volunteers were given sublingual vitamin D3 (60,000IU) in six doses every fifteen days of follow up for 3 months. The subject’s serum 25(OH)D levels were estimated before and after the treatment of sublingual vitamin D3. There was a statistically significant difference in serum vitamin D3 level before 16.61±6.71 ng/ml and after 35.80±7.80 ng/ml after treatment with Sublingual Vitamin D3. Six doses of 60,000IU of Vitamin D3 sublingual route having improved the role of serum 25(OH)D levels in the treatment of Vitamin D3 deficiency patients.Keywords: Vitamin D3; Sublingual route

scholarly journals The Essential Role of Vitamin D in the Biosynthesis of Endogenous Antimicrobial Peptides May Explain Why Deficiency Increases Mortality Risk in COVID-19 Infections

2020 ◽  
Author(s):  
Patrick J. McCullough ◽  
Jeffrey Amend ◽  
William P. McCullough ◽  
Steven J. Repas ◽  
Jeffrey B. Travers ◽  
...  
Keyword(s):  
Vitamin D ◽  
Antimicrobial Peptides ◽  
Vitamin D3 ◽  
Skin Pigmentation ◽  
Sun Exposure ◽  
Ultraviolet B ◽  
Whole Body ◽  
High Risk Population ◽  
Uvb Radiation ◽  
Vitamin D Receptors

Abstract: A primary action of vitamin D is regulation of gene transcription. Many cell types possess genes that make antimicrobial peptides (AMPS) (endogenous antibiotics), recently discovered to be regulated by vitamin D. Two examples are cathelicidin and beta defensins, both bioactive against many different bacteria, fungi, mycobacteria, parasites and viruses. The signal transduction pathway is triggered by sensing microorganisms via cell surface receptors, causing intracellular production of calcitriol (1,25(OH)2D) and vitamin D receptors, leading to upregulation of AMP production. Serum 25(OH)D concentrations required to sustain adequate AMP production to eradicate infections are unknown. Vitamin D3 is photosynthesized in skin in amounts ranging from 10,000 (250 mcg) to 25,000 (625 mcg) International Units (IU) from 7-dehydrocholesterol after whole-body exposure to one minimal erythemal dose (MED) of ultraviolet B (UVB) radiation, and is impacted by many factors including geographic localities, seasonal changes and skin pigmentation. We and others have reported extended daily oral dosing with these amounts of vitamin D3 safe. We routinely observe serum 25(OH)D concentrations below 20ng/ml on new admissions, which have been reported insufficient to sustain AMP production. In contrast serum 25(OH)D concentrations above 100ng/ml have been reported after serial UVB treatments for psoriasis. Little vitamin D naturally occurs in food, and insufficient sun exposure may be causing worldwide deficiency. We review evidence suggesting that higher daily intakes of vitamin D3 than the currently recommended 600 (15 mcg) IU/day may be necessary to sustain AMP production in the face of an overwhelming infection, particularly in non-Hispanic blacks, a high risk population suffering the worst outcomes from COVID-19. We propose that increased vitamin D supplementation could provide a safe and cost-effective way to protect all populations from infections, in particular those from pandemic COVID-19.

scholarly journals Transcriptional activation of the wild-type and mutant vitamin D receptors by vitamin D3 analogs

2016 ◽  
Vol 57 (1) ◽  
pp. 23-32
Author(s):  
Kumi Futawaka ◽  
Tetsuya Tagami ◽  
Yuki Fukuda ◽  
Rie Koyama ◽  
Ayaka Nushida ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D3 ◽  
Transcriptional Activation ◽  
Target Genes ◽  
Active Form ◽  
Therapeutic Effects ◽  
Wild Type ◽  
Human Embryonic Kidney Cells ◽  
Vitamin D Receptors ◽  
High Doses

The active form of vitamin D3 (1α,25(OH)2D3, also known as calcitriol) controls the expression of target genes via the vitamin D receptor (VDR). Vitamin D-dependent rickets type II (VDDRII) is a congenital disease caused by inactivating mutations in the VDR. The condition is treated with high doses of calcitriol, but the therapeutic effects of other synthetic VD3 analogs have not yet been investigated. In the present study, we analyzed the transcriptional activity of seven different VD3 analogs with VDRs carrying ligand-binding domain mutations identified in VDDRII patients. Wild-type VDR (WT-VDR) and seven mutant VDRs were expressed in TSA201 human embryonic kidney cells, HepG2 human liver cancer cells, and MC3T3-E1 mouse calvaria cells, and their transcriptional activation with VD3 analogs were analyzed by performing transient expression assays, western blotting, and quantitative real-time PCR. The results demonstrated that falecalcitriol stimulated significantly higher transcriptional activation of the WT-VDR and some mutant VDRs than did calcitriol. Calcitriol showed almost no transcriptional activation of the VDR with the I268T mutation identified in a severe case of VDDRII, whereas falecalcitriol caused a dose-dependent increase in the activation of this mutant VDR. Our findings demonstrate that falecalcitriol has a VDR activation profile distinct from that of calcitriol and may exhibit therapeutic effects even on difficult-to-treat VDDRII cases resistant to calcitriol. It is also possible that VDDRII patients responding to high doses of calcitriol could be appropriately treated with low doses of falecalcitriol.

scholarly journals The Relationship between Serum Vitamin D Level and Psychosomatic Symptoms in Females in Mosul City

2021 ◽  
Author(s):  
Janan A. Alrefaee ◽  
Ban K. Rushdi ◽  
Ghada K. Adeeb
Keyword(s):  
Vitamin D ◽  
Vitamin D3 ◽  
Serum Vitamin ◽  
Serum Levels ◽  
The Body ◽  
Reproductive Age ◽  
Mild Depression ◽  
Psychosomatic Symptoms ◽  
Serum Vitamin D ◽  
Before And After

Background: A widespread severe deficiency of vitamin D (vit D) exists in Iraq, especially among women of reproductive age. Depression or anxiety is related to deficiency of vit D. These patients may present with psychosomatic symptoms which are symptoms with no sufficient organic cause in the body but from psychological causality. The study aimed to investigate the serum level of vit D in females with psychosomatic symptoms residing in Mosul City, Iraq and explore the effect of vitamin D3 (vit D3) supplements for treating psychosomatic symptoms. Methods: A total of 73 female patients aged 15–45 years presented with several symptoms. All patients underwent physical examination and psychiatric interviews. Patients with minimal to mild depression were selected. All included patients underwent investigation of first serum vit D level. The supplementation with vitamin D3 was started. The second serum vit D level was investigated at first post-improvement visit. Results: In the improved group, the post-supplementation serum vit D level was higher than that before the supplementation (p = 0.00). In addition, the vit D serum difference before and after its supplementation was greater in the improved group than the nonimproved one (p = 0.00). Patients not only experienced improvement in their mood swings, but also recovered from other recorded symptoms such as headache, fatigue, tiredness, and joint pain. Conclusion: This study revealed a noticeable association concerning serum levels of 25-OH D and mild depression in addition to psychosomatic symptoms in females living in Mosul City. Supplementation with suitable loading vit D3 dosages improved these symptoms showing a probable causal relationship.

scholarly journals Impact of Vitamin D supplementation on Heart Rate Variability in Vitamin D deficient Asthma COPD Overlap Syndrome patients: A Randomized Controlled Trial

2020 ◽  
Vol 15 (2) ◽  
pp. 98-107
Author(s):  
Salsa Bill Nahar ◽  
Sultana Ferdousi ◽  
Shamima Sultana ◽  
Kazi Saifuddin Bennoor ◽  
Mustafijur Rahman ◽  
...  
Keyword(s):  
Heart Rate ◽  
Vitamin D ◽  
Heart Rate Variability ◽  
Vitamin D3 ◽  
Time Domain ◽  
Controlled Trial ◽  
Serum Vitamin ◽  
Vitamin D Supplementation ◽  
Overlap Syndrome ◽  
Before And After

Background:Vitamin D3 deficiency is common in Asthma COPD overlap syndrome (ACO) and administration of vitamin D3 increased heart rate variability in healthy subjects. Objective: This randomized clinical trial aimed to investigate the therapeutic effect of vitamin D3administration on time domain measures of heart rate variability in 51 male vitamin D3(<30- 10ng/ml) deficient ACO patients. Methods: Patients were given either vitaminD3 capsule or placebo per week orally for 3 months and serum vitamin D3 level and time domain parameters of heart rate variability were assessed before and after the intervention. For statistical analysis, independent and paired sample t test was used. Results: Before intervention, vitamin D3 level and time domain parameters of heart rate variability were similar in all patients but these outcome measures significantly increased in vitamin D3 treated but not in placebo treated patients after 3 months. Conclusion:In conclusion, vitaminD3therapy is effective to improve heart rate variability in vitamin D3 deficient Asthma COPD overlap syndrome. J Bangladesh Soc Physiol. 2020, December; 15(2): 98-107

scholarly journals Study of association between hypovitminosis D and fibroid uterus

2021 ◽  
Vol 10 (6) ◽  
pp. 2432
Author(s):  
Sangeeta Chowdhary ◽  
Mala Shukla ◽  
Ruchi Joshi
Keyword(s):  
Vitamin D ◽  
Vitamin D3 ◽  
Financial Burden ◽  
Critical Role ◽  
Serum Vitamin ◽  
P Value ◽  
Vitamin D Receptors ◽  
Fibroid Uterus ◽  
Vitamin D Levels ◽  
Prospective Longitudinal

Background: Fibroid uterus or leiomyoma is a benign tumour composed mainly of unicellular smooth muscle cells with varying amounts of fibrous connective tissue. UF are associated with significant morbidity as it presents in form of abnormal uterine bleeding, anaemia, pelvic pain, subfertility, and obstetric complications and causes financial burden on the patient. Recent studies have shown the critical role that vitamin D plays in fibroid formation, with individual fibroids expressing lower levels of vitamin D receptors than adjacent healthy tissue, making vitamin D deficiency a crucial, yet preventable risk factor.Methods: This was cross-sectional observational study. It was conducted on 140 female patients aged (18 to 50 years) presenting to the OPD of department of obstetrics and gynecology at NDMC & Hindu Rao Hospital during July 2019 to June 2020. 70 women had uterine fibroids on ultrasound and treated as cases and rest healthy women without fibroids served as controls. All women were subjected to ultrasound examination of uterus followed by serum vitamin D3 levels.Results: The mean value of vitamin D levels in cases was 12.81±8.56 ng/ml and in controls it was 19.83±9.21 ng/ml with p value<0.0001. Thus, it was statistically significant lower in cases of fibroid uterus as compared to controls. Secondary incidental outcomes were found between vitamin D3 level and BMI as fibroids occur statistically significantly more often in patient having of BMI ≥25 kg/m2. Also, menorrhagia and dysmenorrhea were the major complaints in 62.5% of cases followed by lowers abdominal pain and dyspareunia.Conclusions: Our study reached significance in the inverse correlation between fibroids and vitamin D levels at the primary outcome level. Larger prospective longitudinal studies drawing on more number and eliminating confounding variables are needed.

scholarly journals Vitamin D3 Enriches Ceramide Content in Exosomes Released by Embryonic Hippocampal Cells

2021 ◽  
Vol 22 (17) ◽  
pp. 9287
Author(s):  
Carmela Conte ◽  
Samuela Cataldi ◽  
Cataldo Arcuri ◽  
Alessandra Mirarchi ◽  
Andrea Lazzarini ◽  
...  
Keyword(s):  
Vitamin D ◽  
Cell Differentiation ◽  
Vitamin D3 ◽  
Cell Communication ◽  
Cellular Functions ◽  
Whole Cells ◽  
Vitamin D Receptors ◽  
Neutral Sphingomyelinase ◽  
Liquid Chromatography Tandem Mass ◽  
Ceramide Content

The release of exosomes can lead to cell–cell communication. Nutrients such as vitamin D3 and sphingolipids have important roles in many cellular functions, including proliferation, differentiation, senescence, and cancer. However, the specific composition of sphingolipids in exosomes and their changes induced by vitamin D3 treatment have not been elucidated. Here, we initially observed neutral sphingomyelinase and vitamin D receptors in exosomes released from HN9.10 embryonic hippocampal cells. Using ultrafast liquid chromatography tandem mass spectrometry, we showed that exosomes are rich in sphingomyelin species compared to whole cells. To interrogate the possible functions of vitamin D3, we established the optimal conditions of cell treatment and we analyzed exosome composition. Vitamin D3 was identified as responsible for the vitamin D receptor loss, for the increase in neutral sphingomyelinase content and sphingomyelin changes. As a consequence, the generation of ceramide upon vitamin D3 treatment was evident. Incubation of the cells with neutral sphingomyelinase, or the same concentration of ceramide produced in exosomes was necessary and sufficient to stimulate embryonic hippocampal cell differentiation, as vitamin D3. This is the first time that exosome ceramide is interrogated for mediate the effect of vitamin D3 in inducing cell differentiation.

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