scholarly journals Cytotoxicity evaluation of fungal-derived silver nanoparticles on human gingival fibroblast cell line: An in vitro study

2019 ◽  
Vol 22 (2) ◽  
pp. 160 ◽  
Author(s):  
KiranR Halkai ◽  
JayashreeA Mudda ◽  
Vasundhara Shivanna ◽  
Veena Patil ◽  
Vandana Rathod ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cell Line ◽  
In Vitro Study ◽  
Fibroblast Cell ◽  
Vitro Study ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cell Line

scholarly journals Comparative Evaluation of Cytotoxicity and Genotoxicity of Two Bioceramic Sealers on Fibroblast Cell Line: An in vitro Study

2018 ◽  
Vol 19 (6) ◽  
pp. 656-661 ◽  
Author(s):  
Moksha Nayak ◽  
CN Vijay Kumar ◽  
Veena Shetty ◽  
Anoop V Nair ◽  
L Krishna Prasada ◽  
...  
Keyword(s):  
Cell Line ◽  
Comparative Evaluation ◽  
In Vitro Study ◽  
Fibroblast Cell ◽  
Vitro Study ◽  
Fibroblast Cell Line

In vitro study for laser gene transfer in BHK-21 fibroblast cell line

2009 ◽  
Author(s):  
M. Abdel Aziz ◽  
D. S. Salem ◽  
M. S. Salama ◽  
Y. Badr
Keyword(s):  
Gene Transfer ◽  
Cell Line ◽  
In Vitro Study ◽  
Fibroblast Cell ◽  
Vitro Study ◽  
Fibroblast Cell Line

scholarly journals Cytotoxicity of acrylic based resin compounds in a human gingival fibroblast cell line

2013 ◽  
Vol 54 (2) ◽  
pp. 87-90
Author(s):  
Mariana Souto-Lopes ◽  
Álvaro Azevedo ◽  
Alexandra Teixeira ◽  
Diana Bastos-Aires ◽  
José Lordelo ◽  
...  
Keyword(s):  
Cell Line ◽  
Fibroblast Cell ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cell Line

scholarly journals Cytotoxic Evaluation of Mala aluation of Malaysian K ysian Kelulut Hone elulut Honey on Human y on Human Gingival Fibr al Fibroblast Cell Line using M oblast Cell Line using MTT Assay

2021 ◽  
Vol 28 (2) ◽  
Author(s):  
Chee Zi Yun ◽  
◽  
Nurul Hafizah Mohd Nor ◽  
Zurairah Berahim ◽  
Kannan Thirumulu Ponnuraj ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cell Line ◽  
Mtt Assay ◽  
Fibroblast Cell ◽  
P Value ◽  
Human Gingival Fibroblast ◽  
Growth Media ◽  
Gingival Fibroblast ◽  
Fibroblast Cell Line ◽  
Time Points

Kelulut honey or stingless bee honey is a type of honey produced by stingless bees of the Trigona species where the nest is found in living trees. Objective: The aim of this study was to evaluate the cytotoxic potential of Malaysian Kelulut honey by employing MTT assay on a human gingival fibroblast cell line. Methods: Human gingival fibroblast cell line was cultured in minimal essential medium alpha (α-MEM) with 10% foetal bovine serum and 1% penicillin-streptomycin solution in a 5% CO2 incubator at 37°C in a humidified atmosphere. The cells were seeded at a cell density of 5x103 cells/well in a 96-well culture plate for 24 hours. The cells were treated with seven different concentrations (200, 100, 50, 25, 12.5, 6.25, and 3.125mg/ml) of Malaysian Kelulut honey and incubated in a CO2 incubator. The negative control comprised cells treated with growth media alone. The cell viability was assessed using MTT assay at 24, 48, and 72 hours. The test plate was shaken using a microplate shaker and the absorbance of the solution was measured at 570nm using an ELISA reader with the Magellan software. Statistical analysis of the data was carried out using Kruskal-Wallis test and SPSS 24.0.0 for Windows. A p value <0.05 was considered as statistically significant. Results: There was no cytotoxic effect of Malaysian Kelulut honey on HGF-1 based on the MTT assay at different concentrations and at different time points tested as the cell viability was above 70%. The highest percentage of cell viability at all three different durations of treatment were observed at 3.125mg/ml, whereas the lowest cell viability was observed at 200mg/ml of Kelulut honey concentration. However, statistically significant differences were seen between some of the concentrations at various time points. Conclusion: Since the cell viability of HGF-1 treated with Malaysian Kelulut honey was more than 70% at all concentrations ranging from 3.125mg/ml to 200mg/ml at three different time points (24, 48 and 72 hours), Malaysian Kelulut honey can be considered as non-cytotoxic on human gingival fibroblasts based on MTT assay under the present test conditions

Effect of cyclosporin A on human gingival fibroblast collagen turnover in relation to the development of gingival overgrowth: an in vitro study

2004 ◽  
Vol 58 (4) ◽  
pp. 231-238 ◽  
Author(s):  
Nicoletta Gagliano ◽  
Claudia Moscheni ◽  
Claudia Dellavia ◽  
Carlo Torri ◽  
Giordano Stabellini ◽  
...  
Keyword(s):  
Cyclosporin A ◽  
In Vitro Study ◽  
Vitro Study ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Collagen Turnover ◽  
Gingival Overgrowth

scholarly journals Apoptosis inducing effects of chlorhexidine and essential oil mouthwashes on BHK-21 fibroblast cell line: An in vitro study

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1703
Author(s):  
Shaimaa Ali Hamouda Ali El Basuony ◽  
Naglaa El Hossary ◽  
Nermine Raouf Amin
Keyword(s):  
Cell Line ◽  
In Vitro Study ◽  
Fibroblast Cell ◽  
Dependent Manner ◽  
Fibroblast Cell Line ◽  
Chemical Agents ◽  
Apoptotic Effects ◽  
Different Doses ◽  
Fibroblast Cell Lines

Background: The maintenance of oral health can be achieved mainly by mechanical and chemical means. Among chemical agents, mouthwashes are widely used for personal oral hygiene because of their ability to inhibit dental plaque. The antibacterial effects of essential oils (EOs) and chlorhexidine (CHX) are well documented; however, the reaction of host tissue to these substances has a poor documentation. Until now studies have not examined the effect of EOs with sodium fluoride (EOF) on fibroblast cell lines. The aim of this study was to examine the effect of mouth rinse EOs, EOF and CHX on the apoptosis of fibroblast cell line. Methods: BHK-21 fibroblast cell line was cultured and incubated in Eagle's Minimum Essential Medium containing EOs, EOF and CHX mouthwashes with different doses (15% or 25%) and various exposure times. Cell apoptosis was assayed using RT-PCR. Results: EOs, EOF and CHX induce apoptotic effects on fibroblasts in a dose and time dependent manner. Conclusion: CHX is the most cytotoxic mouthwash to fibroblasts as compared to mouthwashes containing EOs and EOF.

scholarly journals Effect of various irrigation solutions on cytokine expression of human gingival fibroblast: in vitro study

2019 ◽  
Vol 15 (2) ◽  
pp. 198-203
Author(s):  
Zeliha Uğur Aydın ◽  
Kerem Engin Akpınar ◽  
Ceylan Hepokur ◽  
Merve Alpay ◽  
Demet Altunbaş
Keyword(s):  
In Vitro Study ◽  
Cytokine Expression ◽  
Vitro Study ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast
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