scholarly journals Identification of suitable reference genes in blood samples of carcinoma lung patients using quantitative real-time polymerase chain reaction

2020 ◽  
Vol 19 (1) ◽  
pp. 11
Author(s):  
Sweety Gupta ◽  
ShashiRanjan Mani Yadav ◽  
Bela Goyal ◽  
Raman Kumar ◽  
Amit Gupta ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Reference Genes ◽  
Chain Reaction ◽  
Blood Samples ◽  
Polymerase Chain ◽  
Suitable Reference

scholarly journals Selection of Suitable Reference Genes for Quantitative Real-time Polymerase Chain Reaction in Prunus mume during Flowering Stages and under Different Abiotic Stress Conditions

2014 ◽  
Vol 139 (2) ◽  
pp. 113-122 ◽  
Author(s):  
Tao Wang ◽  
Ruijie Hao ◽  
Huitang Pan ◽  
Tangren Cheng ◽  
Qixiang Zhang
Keyword(s):  
Polymerase Chain Reaction ◽  
Abiotic Stress ◽  
Real Time ◽  
Reference Genes ◽  
Late Embryogenesis Abundant Protein ◽  
Prunus Mume ◽  
Chain Reaction ◽  
Qrt Pcr ◽  
Polymerase Chain ◽  
Suitable Reference

Mei (Prunus mume) is widely cultivated in eastern Asia owing to its favored ornamental characteristics and its tolerance for low temperatures. Reverse transcription quantitative real-time polymerase chain reaction (qRT-PCR) is a widely used method for gene expression analysis, requiring carefully selected reference genes to ensure data reliability. The aim of this study was to identify and evaluate reference genes for qRT-PCR in mei. Ten candidate reference genes were chosen, and their expression levels were assessed by qRT-PCR in four sample sets: 1) flowering mei; 2) mei undergoing abiotic stress; 3) different genotypes of Prunus species; and 4) all mei samples. The stability and suitability of the candidate reference genes were validated using commercially available software. We found that protein phosphatase 2A-1 (PP2A-1) and PP2A-2 were suitable reference genes for flowering with ubiquitin-conjugating enzyme E2 (UBC) also being suitable for different genotypes of Prunus species. UBC and actin (ACT) were most stably expressed under abiotic stress. Finally, the expression of an AGAMOUS homolog of Arabidopsis thaliana (PmAG) and a putative homolog of Group 2 late embryogenesis abundant protein gene in A. thaliana (PmLEA) were assessed to allow comparisons between selected candidate reference genes, highlighting the importance of careful reference gene selection.

Validation of Reference Genes for Real-Time Polymerase Chain Reaction Studies in Atlantic Salmon

2006 ◽  
Vol 8 (4) ◽  
pp. 398-408 ◽  
Author(s):  
Sven Martin Jorgensen ◽  
Ellen Johanne Kleveland ◽  
Unni Grimholt ◽  
Tor Gjoen
Keyword(s):  
Polymerase Chain Reaction ◽  
Atlantic Salmon ◽  
Real Time ◽  
Reference Genes ◽  
Chain Reaction ◽  
Polymerase Chain

Validation of reference genes for quantitative real‐time polymerase chain reaction in Drosophila melanogaster exposed to two chemicals

2019 ◽  
Vol 49 (6) ◽  
pp. 277-283 ◽  
Author(s):  
YeongHo Kim ◽  
YiSeul Kim ◽  
Donghun Kim ◽  
SeYeon Kim ◽  
GyeongJin Seo ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Drosophila Melanogaster ◽  
Real Time ◽  
Reference Genes ◽  
Chain Reaction ◽  
Polymerase Chain
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