scholarly journals Quantitative estimation of urinary ortho-cresol by high pressure liquid chromatography for biomonitoring of toluene exposed population

2020 ◽  
Vol 5 (3) ◽  
pp. 78
Author(s):  
Anupa Yadav ◽  
Amit Chakrabarti ◽  
Geoffrey Nengzapum
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Quantitative Estimation ◽  
Exposed Population ◽  
Ortho Cresol

Eine quantitative Bestimmungsmethode des Uracilgehaltes biologischen Materials im Nanogramm-Bereich mit Hilfe der Hochdruck-Flüssigchromatografie / A Method for the Quantitative Estimation of Uracil Content in Biological Materials in the Nanogramme-Range Using High-Pressure Liquid Chromatography

1974 ◽  
Vol 29 (3-4) ◽  
pp. 139-141 ◽  
Author(s):  
Hans-Joachim Breter ◽  
Marianne Schnaus ◽  
Werner E. G. Müller ◽  
Rudolf K. Zahn
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Quantitative Estimation ◽  
Biological Materials ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Isotope Dilution Analysis ◽  
Highly Sensitive ◽  
Hydrolysis Of

Abstract A method is described for the estimation of the uracil content in biological materials by means of high-pressure liquid chromatography. Hydrolysis of the tissues and total liberation of RNA bases are carried out in 70% perchloric acid. Less than 1 mg of the materials are needed for analysis. A pre-purification of the hydrolyzates is carried out by anion-exchange chromatography. Recoveries are estimated by isotope dilution analysis with [2-14C] labelled uracil. The method is highly sensitive - about 6000 pmol of uracil content can at least be estimated quantitatively - and analysis time is short. In routine analysis a single sample needs 4 hours to be completed. When preparing several samples at a time an analysis is completed every 45min.

Historical chemical annotations of Cinchona bark collections are comparable to results from current day high-pressure liquid chromatography technologies

2020 ◽  
Vol 249 ◽  
pp. 112375 ◽  
Author(s):  
Nataly Allasi Canales ◽  
Tobias Nikolaj Gress Hansen ◽  
Claus Cornett ◽  
Kim Walker ◽  
Felix Driver ◽  
...  
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography
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