scholarly journals Clinical Comparison of Fissure Sealants Retention Following Preparation with Seventh Generation Bonding Agent with Prior Etching and Conventional Acid Etch and Bond System (fifth Generation)

2021 ◽  
Vol 12 (1) ◽  
pp. 22
Author(s):  
Azin Sohrabi ◽  
Leila Erfanparast ◽  
Masoumeh Vatandoust ◽  
Leila Mahmoudi
Keyword(s):  
Bonding Agent ◽  
Fissure Sealants ◽  
Fifth Generation ◽  
Clinical Comparison ◽  
Bond System ◽  
Acid Etch

scholarly journals In vitro antibacterial effect of fifth generation dentin bonding agent incorporated with nisin on Streptococcus mutans

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Gopal Keerthipriya ◽  
Nesamani Ravikumar ◽  
Sekar Mahalaxmi
Keyword(s):  
Streptococcus Mutans ◽  
Optical Density ◽  
Bacterial Growth ◽  
Membrane Integrity ◽  
Bonding Agent ◽  
Control Group ◽  
Dentin Bonding ◽  
Fifth Generation ◽  
Live Bacteria ◽  
Dentin Bonding Agent

Abstract Introduction Bacterial adherence to restorative materials such as composite resin is one of the aetiology of secondary caries. This study evaluated the antibacterial efficacy of fifth generation bonding agent (BA) modified with nisin, against Streptococcus mutans based on its growth, adherence and membrane integrity. Methods Adhesive eluents of the experimental bonding agents were obtained using 250 μl Brain Heart Infusion (BHI) broth and the groups were control (BA with 0% Nisin), bonding agent with 1 wt% (NBA 1) and 5 wt% nisin (NBA 5). To this, 10 μl S. mutans culture was added and incubated at 37 °C. Bacterial growth was estimated by changes in optical density using spectrophotometer every 20 min for 2 h. The results were statistically analysed using one way ANOVA followed by Tukey Post Hoc test. For adherence and membrane integrity test, 10 μl of BHI supplemented with 1% sucrose and 50 μl of bacterial suspension were inoculated onto the cured specimens, and incubated for 4 h. After rinsing, 1 ml of Live/Dead BacLight bacterial viability stain was added and incubated in the dark for 15 min and observed under confocal laser scanning microscope (CLSM) for intact (green/live) and damaged (red/dead) bacterial membranes. Results Mean optical density was significantly higher in control group at all time intervals with maximum value at 2 h (0.83 ± 0.008), while there was a concentration dependant reduction in bacterial growth with the NBA groups (0.50 ± 0.007). Correspondingly, the NBA groups showed higher amount of dead than live bacteria, while live bacteria were predominant in the control group. Significance Addition of an antibacterial agent nisin in dentin bonding agent may render the resin dentin interface more resistant to bacterial penetration, and adherence of cariogenic bacteria like S. mutans.

An Investigation to Estimate the Flouride Uptake Adjacent to a Fluoride-releasing Bonging Agent

1995 ◽  
Vol 22 (2) ◽  
pp. 113-122 ◽  
Author(s):  
S. M. Chadwick ◽  
P. H. Gordon
Keyword(s):  
At Risk ◽  
Clinical Significance ◽  
Orthodontic Treatment ◽  
Bonding Agent ◽  
Bonding Agents ◽  
Acid Etch ◽  
Fixed Appliances

Decalcification of the teeth remains a problem during orthodontic treatment with fixed appliances. It has been suggested that bonding agents which release fluoride could supply it to the area of the tooth most at risk from decalcification. The aim of this study was to estimate uptake by enamel adjacent to a fluoride releasing bonding agent. Acid etch biopsies were used to estimate the concentration of fluoride in enamel adjacent to brackets bonded with Vitrabond® and Geristore®. Results indicate that there was a significant increase in the concentration of fluoride in enamel adjacent to Vitrabond®. The clinical significance of the increase in the concentration of fluoride adjacent to Vitrabond® and the mechanism by which fluoride moves from the material into the enamel remain unclear.

scholarly journals A comparison of microleakage in self-etch fissure sealants and conventional fissure sealants with total-etch or self-etch adhesive systems

2018 ◽  
Vol 12 (02) ◽  
pp. 242-246 ◽  
Author(s):  
Azam Nahvi ◽  
Alireza Razavian ◽  
Hoorieh Abedi ◽  
Jamshid Yazdani Charati
Keyword(s):  
In Vitro Study ◽  
Conventional Technique ◽  
Single Bond ◽  
Adhesive Systems ◽  
Fissure Sealants ◽  
Fissure Sealant ◽  
Acid Etch ◽  
Self Etch ◽  
Group 1

ABSTRACT Objective: The present study was conducted to compare microleakage in self-etching fissure sealants and conventional fissure sealants with total-etch or self-etch adhesive systems. Settings and Design: This experimental in vitro study was conducted on 60 healthy third molars extracted from humans. The first group received Acid etch + Clinpro sealant, the second group received Acid etch + Single bond 2 + Clinpro sealant, the third group received Single bond universal (self-etching bonding) + Clinpro sealant, and the fourth group received prevent seal self-etching sealant. Materials and Methods: An incision was made on the teeth after they were immersed in methylene blue 5%. The samples were then examined under a stereomicroscope and the dye penetration rate was measured based on the Williams and Winter criteria. Statistical Analysis Used: The Kruskal–Wallis and Mann–Whitney tests were used for data analysis in SPSS-18 (P < 0.05). Results: Group 1 which was treated with the conventional technique (acid + fissure sealant) had the highest rate of microleakage compared to Groups 2 and 3 (P < 0.001). Conclusion: The results showed that the use of bonding results in a significant reduction in the microleakage of fissure sealants. The microleakage caused when using self-etch fissure sealant was not different from that caused by the use of the conventional method.

Evaluation of Shear Bond Strength and SEM Observation of All-in-one Self-etching Primer Used for Bonding of Fissure Sealants

2006 ◽  
Vol 7 (2) ◽  
pp. 9-16 ◽  
Author(s):  
Maha Abdulla Al-Sarheed
Keyword(s):  
Phosphoric Acid ◽  
Bond Strength ◽  
Shear Bond Strength ◽  
Testing Machine ◽  
Fissure Sealants ◽  
Fissure Sealant ◽  
Sem Observation ◽  
Instron Testing Machine ◽  
Acid Etch ◽  
The Mean

Abstract Objectives To evaluate and compare enamel shear bond strength of an all-in-one self-etching primer (Prompt™ L-Pop™) to regular acid etch material when bonded to two-fissure sealant systems (Concise and Dyract Seal). Method and Materials Forty newly extracted non-carious first permanent molars were embedded in a Teflon mold. The teeth were divided into four groups and each consisted of ten specimens. The bonding surfaces were treated with either Prompt L-Pop as recommended by the manufacturer or etched with phosphoric acid. After 24 hours of water storage, the specimens were evaluated for shear bond strength using an Instron testing machine. Scanning electron microscope (SEM) examinations were carried out to evaluate the failure sites of the sealants. Results The mean shear bond strengths using Prompt L-Pop were Concise: 23.46 MPa and Dyract Seal: 20.34 MPa. These values were higher than and statistically different from those of Dyract Seal (9.99 MPa) and Concise (8.85 MPa) when phosphoric acid was used. The failure was predominantly of the adhesive type. The SEM examination showed the failure of debonding was predominantly related to the type of etching systems used rather than the type of fissure sealants. Conclusion The use of the all-in-one self-etching adhesive Prompt L-Pop improves the mean enamel shear bond strength of fissure sealants. Citation Al-Sarheed MA. Evaluation of Shear Bond Strength and SEM Observation of All-in-one Self-etching Primer Used for Bonding of Fissure Sealants. J Contemp Dent Pract 2006 May;(7)2:009-016.

scholarly journals In Vitro Antibacterial Effect of Fifth Generation Dentin Bonding Agent Incorporated With Nisin on Streptococcus Mutans

2021 ◽  
Author(s):  
Gopal Keerthipriya ◽  
Nesamani Ravikumar ◽  
Sekar Mahalaxmi
Keyword(s):  
Streptococcus Mutans ◽  
Optical Density ◽  
Bacterial Growth ◽  
Membrane Integrity ◽  
Bonding Agent ◽  
Control Group ◽  
Integrity Test ◽  
Secondary Caries ◽  
Fifth Generation ◽  
Live Bacteria

Abstract Introduction Secondary caries is the most common cause of failure of composite restorations. This study evaluated the antibacterial efficacy of fifth generation bonding agent (BA) modified with Nisin, a polypeptide bacteriocin against Streptococcus mutans based on its growth, adherence and membrane integrity. Methods Adhesive eluents of the three experimental bonding agents, namely control BA only, bonding agent with 1 wt% Nisin (NBA 1) and 5 wt% Nisin (NBA 5) were obtained using 250 μl Brain Heart Infusion (BHI) broth. To this, 10 μl S. mutans culture was added and incubated at 37°C. Bacterial growth was estimated by changes in optical density using spectrophotometry every 20 min for 2 hours. The results were statistically analysed using one way ANOVA followed by Post Hoc test. For adherence and membrane integrity test, 10μl of BHI supplemented with 1% sucrose and 50 μl of bacterial suspension were inoculated onto the cured specimens, and incubated for 4 hours. After rinsing, 1ml of Live/Dead BacLight bacterial viability stain was added and incubated in the dark for 15 min and observed under CLSM for intact (green/live) and damaged (red/dead) bacterial membranes. Results Mean optical density was significantly higher in control group at all time intervals, with experimental groups showing concentration dependant reduction in bacterial growth. Correspondingly, the experimental groups showed higher amount of dead than live bacteria, while live bacteria were predominant in the control group. Significance Addition of an antibacterial agent Nisin in bonding agent may render the resin dentin interface more resistant to bacterial penetration, thus reducing the incidence of secondary caries.

scholarly journals Effect of Enamel Pre-etching with Sodium Hypochlorite Deproteinisation and Bonding Agent on Retention and Microleakage of Pit and Fissure Sealants: An In-vitro Study

2021 ◽  
Author(s):  
VV Mubeena ◽  
Tharian B Emmatty ◽  
Kumar Kavita ◽  
Bijimole Jose ◽  
AM Riswana
Keyword(s):  
Sodium Hypochlorite ◽  
Artificial Saliva ◽  
Medical Science ◽  
Enamel Surface ◽  
Bonding Agent ◽  
Permanent Teeth ◽  
Acid Etching ◽  
Fissure Sealants ◽  
Pit And Fissure Sealants

Introduction: Sealants have proved to be one of the easiest methods of caries prevention in young permanent teeth, the placement of which is very technique sensitive. The non-invasive method of pretreating the enamel surface by deproteinising it with 5.25% sodium hypochlorite (NaOCl) for 60 seconds prior to etching has proven to be a promising method for improving retention and reducing microleakage of sealant. Aim: To evaluate the effect of enamel pre-etching with sodium hypochlorite deproteinisation and bonding agent on retention and microleakage of pit and fissure sealants. Materials and Methods: This in-vitro experimental study was conducted in Department of Paediatric and Preventive Dentistry at Annoor Dental College, Muvattupuzha, Kerala and Nanotechnology Lab at Amrita Institute of Medical Science. Freshly extracted Intact permanent maxillary and mandibular 20 third molars and 20 premolars mounted on acrylic blocks were divided into 4 groups containing 5 teeth in each group. Molars and premolar were taken for assessing the rate of retention and microleakage, respectively. Teeth in Group 1 were subjected to acid etching only, Group 2 were deproteinised prior to acid etching, Group 3 were subjected to acid etching followed by bonding and Group 4 were subjected to deproteinisation, etching and bonding. Then, all teeth were sealed with pit and fissure sealant and underwent thermocycling in artificial saliva bath for evaluation of sealant retention and microleakage. After thermocycling, retention was assessed on molar by one pre-calibrated blinded examiner by passing a 0.5 mm diameter probe along the margins of the sealant placed to verify integrity, failure, or loss of continuity based on Simonsen’s criteria (1989). To assess microleakage, premolar samples were immersed in rhodamine B, sectioned longitudinally, and examined under a stereomicroscope for assessment of microleakage. Collected data were statistically analysed using Chi-square and Mann-Whitney u test. Results: The results showed 100% retention for teeth in Group 2a, Group 3a, Group 4a, while partial sealant loss was present in 3 teeth from Group 1a. The differences between the groups for retention was statistically significant (p=0.014). In the case of microleakage, Group 3b showed more microleakage and group 4b showed least microleakage and the difference between them was statistically significant (p=0.009). Conclusion: Deproteinisation and use of bonding agent increases retention of the pit and fissure sealants. However, deproteinisation is a more effective method to control microleakage when compared to bonding agent. Thus, it can be considered as an effective method for pretreating enamel surface before sealant placement.

scholarly journals Effect of Precuring and Postcuring of Total-etch and Self-etch Bonding Agents on the Microleakage of Fissure Sealants

2020 ◽  
Author(s):  
Bahman Seraj ◽  
Ghasem Meighani ◽  
Shabnam Milani ◽  
Mostafa Fatemi
Keyword(s):  
Pediatric Dentistry ◽  
Bonding Agent ◽  
Control Group ◽  
Single Bond ◽  
Bonding Agents ◽  
Fissure Sealant ◽  
The Third ◽  
Fifth Generation ◽  
Significant Difference ◽  
Self Etch

Objectives: Considering the importance of timesaving in pediatric dentistry, if the efficacy is achieved along with shorter working time and less technical sensitivity, the behavior management of young patients can be anticipated. This study aimed to compare the effect of precuring and postcuring of total-etch and self-etch bonding agents on the microleakage of sealants. Materials and Methods: This study was conducted on forty impacted third molars, which were surgically extracted. The samples were divided into five groups: 1. Control (etching and sealant), 2. Precured fifth-generation bonding agent (Adper Single Bond 2) and fissure sealant, 3. Postcured fifth-generation bonding agent (Adper Single Bond 2) and fissure sealant, 4. Precured seventh-generation bonding agent (Single Bond Universal) and fissure sealant, and 5. Postcured seventh-generation bonding agent (Single Bond Universal) and fissure sealant. All specimens were thermocycled (×1000), immersed in 0.5% basic fuchsine solution for 24 hours, sectioned, digitally photographed, and measured using the LAS EZ program. The data were analyzed using Kruskal-Wallis and Dunn’s tests. Results: Leakage in the control group and the third group was significantly lower than that in other groups. In pairwise comparisons, a significant difference was found between the control group and the fifth group and between the third group and the fifth group. Conclusion: The conventional method of sealant placement showed superior results in comparison with the use of an intermediate layer of the bonding agent.

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