scholarly journals Phytochemical screening, antimicrobial and antioxidant studies of Lannea egregia Engl. and K. Krause (Anacardiaceae) stem bark

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Basirat O. Rafiu ◽  
Adeola M. Sonibare ◽  
Enitan O. Adesanya
Keyword(s):  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Standard Procedure ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Skin Disorders ◽  
Bacterial Strains ◽  
Fungal Strains

Background: A substantial number of drugs are being developed from plants for the treatment of various diseases. Lannea egregia (LE) is a woody perennial plant used traditionally in the management of skin disorders and wounds.Aim: To investigate the biological activities of different solvent extracts of LE bark.Settings: Fresh stem bark of Lannea egregia was collected from Itabo Lanlate, Oyo State, Nigeria. The authentication was done in Forest Herbarium, Ibadan (FHI), Nigeria. The biological activities of the air-dried sample were carried out in the Department of Pharmacognosy, University of Ibadan, Nigeria.Materials and methods: Powdered LE bark sample was extracted by gradient extraction. Phytochemical screening was performed on the extracts using standard procedure. In vitro antimicrobial study was performed on 14 strains of bacteria and 5 fungal strains at a concentration range of 25, 50, 75 and 100 mg/mL of the extract using ciprofloxacin and itraconazole as standard. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) were determined. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and total phenolic content (TPC) of two active extracts were determined spectrophotometrically.Results: The phytochemical screening of LE revealed the presence of tannins, terpenoids, flavonoids, anthraquinones, saponins and alkaloids. The dichloromethane (DCM) extract exhibited the highest activity against all the bacterial strains as well as four of the fungal strains. The zones of inhibition (ZI) of bacteria ranged from 9.0 ± 2.0 to 24.6 ± 2.4 mm, MIC of 0.0008 to 12.5 mg/mL and MBC of 25 to 75 mg/mL, while ZI of the fungal strains ranged from 10.3 ± 4.6 to 18.0 ± 5.3 mm, MIC 0.391 – 0.781 and MFC of 50 mg/mL in all the strains. The TPC values of DCM and ethyl acetate (EtOAc) extracts were 1582.47 ± 6.69 and 1579.89 ± 12.77 µg GAE/mg and inhibitory antioxidant activity of 2.54 ± 0.58 and 2.44 ± 0.54, respectively.Conclusion: These findings provide scientific evidence to support the ethnomedicinal use of Lannea egregia bark for treating skin disorders and wounds.

scholarly journals Determination of Phenolic Compounds in Various Propolis Samples Collected from an African and an Asian Region and Their Impact on Antioxidant and Antibacterial Activities

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4589
Author(s):  
Soumaya Touzani ◽  
Hamada Imtara ◽  
Shankar Katekhaye ◽  
Hamza Mechchate ◽  
Hayat Ouassou ◽  
...  
Keyword(s):  
Cinnamic Acid ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Reducing Power ◽  
Antibacterial Activities ◽  
Total Phenolic ◽  
Bacterial Strains ◽  
Photodiode Array Detection

The biological activities of propolis samples are the result of many bioactive compounds present in the propolis. The aim of the present study was to determine the various chemical compounds of some selected propolis samples collected from Palestine and Morocco by the High-Performance Liquid Chromatography–Photodiode Array Detection (HPLC-PDA) method, as well as the antioxidant and antibacterial activities of this bee product. The chemical analysis of propolis samples by HPLC-PDA shows the cinnamic acid content in the Palestinian sample is higher compared to that in Moroccan propolis. The results of antioxidant activity demonstrated an important free radical scavenging activity (2,2-Diphenyl-1-picrylhydrazyl (DPPH); 2,2′-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and reducing power assays) with EC50 values ranging between 0.02 ± 0.001 and 0.14 ± 0.01 mg/mL. Additionally, all tested propolis samples possessed a moderate antibacterial activity against bacterial strains. Notably, Minimum Inhibitory Concentrations (MICs) values ranged from 0.31 to 2.50 mg/mL for Gram-negative bacterial strains and from 0.09 to 0.125 mg/mL for Gram-positive bacterial strains. The S2 sample from Morocco and the S4 sample from Palestine had the highest content of polyphenol level. Thus, the strong antioxidant and antibacterial properties were apparently due to the high total phenolic and flavone/flavonol contents in the samples. As a conclusion, the activities of propolis samples collected from both countries are similar, while the cinnamic acid in the Palestinian samples was more than that of the Moroccan samples.

scholarly journals Phytochemical Screening, Free Radical Scavenging and α-Amylase Inhibitory Activities of Selected Medicinal Plants from Western Nepal

Medicines ◽  
2019 ◽  
Vol 6 (2) ◽  
pp. 70 ◽  
Author(s):  
Kusum Sai ◽  
Rashmi Thapa ◽  
Hari Prasad Devkota ◽  
Khem Raj Joshi
Keyword(s):  
Free Radical ◽  
Medicinal Plants ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Inhibitory Activities

Background: More than 700 plants are reported to be used for medicinal purposes in Nepal; however, many of them are not studied for their scientific evidences. The aims of the present study were the estimation of the total phenolic and flavonoid contents, and the evaluation of the free radical scavenging and α-amylase inhibitory activities of five selected medicinal plants from western Nepal: Aeschynanthus parviflorus Wall. (Gesneriaceae), Buddleja asiatica Lour. (Loganiaceae), Carica papaya L. (Caricaceae), Drepanostachyum falcatum (Nees) Keng f. (Gramineae) and Spondias pinnata (L. f.) Kurz (Anacardiaceae). Methods: The total phenolic content (TPC) and total flavonoid content (TFC) were measured using Folin-Ciocalteu’s phenol reagent and aluminium chloride methods, respectively. A 1,1–diphenyl–2–picrylhydrazyl (DPPH) free radical scavenging assay was used to evaluate the free radical scavenging activity and an α-amylase inhibitory assay was carried out to determine the in vitro antidiabetic activity. Results: The phytochemical screening of five hydroalcoholic plant extracts revealed the presence of various secondary metabolites, including alkaloids, flavonoids, reducing sugars, saponins, terpenoids and tannins. The amounts of total phenolics and flavonoids were found to be the highest in B. asiatica leaf extract, which also showed the most potent free radical scavenging activity. Extract of C. papaya fruits showed the highest α-amylase inhibitory activity, whereas the extracts of B. asiatica leaves and S. pinnata leaves exhibited moderate activity. Conclusions: Some of the medicinal plants selected in this study showed high TPC and TFC values and potent bioactivities. These results may provide the scientific evidences of the traditional uses of these plants. However, further detailed studies on bioactive compounds isolation and identification and evaluation of in vivo pharmacological activities should be performed in future.

scholarly journals PHYTOCHEMICAL SCREENING AND ANTIOXIDANT POTENTIAL OF ANACARDIUM OCCIDENTALE, ACHYRANTHES ASPERA, AND AEGLE MARMELOS

2019 ◽  
pp. 202-205
Author(s):  
BRIJYOG ◽  
LALITESHWAR PRATAP SINGH ◽  
SUSHIL KUMAR ◽  
SHWETA VERMA
Keyword(s):  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Anacardium Occidentale ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Aegle Marmelos ◽  
Hydroalcoholic Extract ◽  
Achyranthes Aspera ◽  
Aerial Parts

Objective: Anacardium occidentale, Achyranthes aspera, and Aegle marmelos are the common plants found in North and South India. The present study identifies the active phytochemicals and antioxidant properties in various extracts derived from dried bark, whole aerial parts, and leaves of the above plants. Materials and Methods: Phytochemical screening included extracts of pet ether, hydroalcoholic, and aqueous. The antioxidant activity was determined by measuring total phenolic contents (TPC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (RSA), and total flavonoid content (TFC). Results: The phytochemical screening of A. occidentale, A. aspera, and A. marmelos of dried bark, whole aerial parts, and leaves revealed the presence of flavonoids, phenols, tannins, and proteins. It also contains alkaloids and glycosides. Hydroalcoholic extract of A. occidentale, A. aspera, and A. marmelos showed highest TPC 0.125 mg/g, 0.256 mg/g, and 0.254 mg/g, respectively. TFCs 0.094 mg/g, 0.145 mg/g, and 0.121 mg/g and highest DPPH RSA with the half maximal inhibitory concentration of 125 μg/mL, 105.58 μg/mL, and 98.89 μg/mL compare to the standard ascorbic acid of 21.65 μg/mL. Conclusion: This study showed that the hydroalcoholic extract of A. occidentale, A. aspera, and A. marmelos is potential source of natural antioxidants.

scholarly journals Biological activities of spike moss Selaginella tamariscina (Beauv.) Spring. collected from Co Thach, Binh Thuan

2019 ◽  
Vol 2 (2) ◽  
pp. 5-13
Author(s):  
Tran Ngoc Bao Do ◽  
Tien Thi My Le ◽  
Hiep Minh Dinh ◽  
Phuong Ngo Diem Quach
Keyword(s):  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Petroleum Ether Extract ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Antibacterial Activities ◽  
Phytochemical Screening ◽  
Reducing Ability ◽  
Whole Plant ◽  
Selaginella Tamariscina

In Vietnam, Selaginella tamariscina (Beauv.) Spring. has been used as a traditional medicine for osteoarthritis, haemorrhage, hepatitis, burn, … However, Selaginella tamariscina is less studied than in other countries. Therefore, this research focuses on study some bioactivities (such as antioxidative, antibacterial activities, and inhibition of albumin denaturation) and preliminary phytochemical screening of six extracts (ethanol extracts of root, leaf, whole plant, petroleum extracts of root, leaf, whole plant) of S. tamariscina collect from Co Thach, Binh Thuan Province. The preliminary phytochemical screening results show that Selaginella tamariscina extracts have most of secondary metabolite groups such as phenolics, alkaloids, flavonoids, steroids, and lactone. The antibacterial activity of the ethanol extract of root is higher than that of other extracts. Moreover, the ethanol extract of root also has the highest reducing ability and free radical scavenging activity, with EC50 = 1.590 ± 0.091mg/ml. In the anti-denaturation of albumin test, the petroleum ether extract of whole plant has the lowest IC50 at 99 ± 5μg/ml.

scholarly journals Tetrapleura tetraptera of Ghanaian Origin: Phytochemistry, Antioxidant and Antimicrobial Activity of Extracts of Plant Parts

2020 ◽  
pp. 78-96
Author(s):  
Christopher Larbie ◽  
Felix Charles Mills- Robertson ◽  
Emmanuel Bright Quaicoe ◽  
Rita Opoku ◽  
Naomi Chounbayor Kabiri ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Scavenging Activity ◽  
Methanolic Extracts ◽  
Healthcare Needs ◽  
Tetrapleura Tetraptera ◽  
Phytochemical Constituents

Introduction: The role of medicinal plants in meeting the healthcare needs of the populace, particularly in developing countries cannot be overemphasized. They provide holistic treatment and wellbeing due to a plethora of phytochemicals found in them. Among such is Tetrapleura tetraptera (Schum. & Thonn.) Taub., a multipurpose tree plant with varied biological activities. The current study was aimed at assessing the basic phytochemical constituents, free radical scavenging activity as well as the antimicrobial effect on various solvent extracts of leaves, fruit and stem bark of T. tetraptera of Ghanaian origin. Materials and Methods: Aqueous, 50% hydroethanolic and methanolic extracts were made from the leaves, fruits, and stem bark of T. tetraptera. They were assessed for the qualitative and quantitative phytochemical constituents, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, antimicrobial activity as well as the spectroscopic properties (Ultraviolet – visible and Fourier-transform infrared spectroscopy). Compounds in the methanolic extracts were evaluated using the gas chromatography–mass spectrometry. Results: Tannins, reducing sugars, coumarins and flavonoids were present in all extracts while hydroethanolic and methanolic extracts were rich in phenols, tannins and flavonoids, especially for the stem bark. Leaf extracts had better antimicrobial activity against Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Candida albicans and Proteus mirabilis. Conclusion: Generally, leaves and stem bark extracts of T. tetraptera are rich in biologically active phytochemicals, supporting their use in ethnomedicine and could be exploited further for other biological activities.

scholarly journals Isolation of phytoconstituents and evaluation of biological potentials of Berberis hispanica from Algeria

2018 ◽  
Vol 13 (2) ◽  
pp. 179 ◽  
Author(s):  
Redouane Lemoui ◽  
Samira Benyahia ◽  
Labib Noman ◽  
Ilham Bencherchar ◽  
Feyza Oke-Altuntas ◽  
...  
Keyword(s):  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Video Clip ◽  
Radical Scavenging ◽  
2D Nmr ◽  
Butylated Hydroxytoluene ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
Remarkable Effect ◽  
Berberis Hispanica

<p class="Abstract">The aim of this study was to isolate the phytoconstituents and evaluate the biological activities of <em>Berberis hispanica</em>. Three phenolic compounds (tamarixetin, caffeic acid and rutin)  were isolated from <em>B. hispanica</em>. The structures of the pure compounds were elucidated by spectroscopic and mass-spectrometric analyses, including 1D-, 2D-NMR, and HPLC-TOF/MS. In addition, antimicrobial, anti-oxidant and anti-proliferative effects of the extracts, some fractions and isolated compounds were evaluated. The extracts of B. hispanica were evaluated against six bacterial strains and exhibited the highest activity against <em>Klebsiella pneumonia</em> (15 mm at 100 mg/mL). Fraction T36 (IC<sub>50</sub>&lt;5 μg/mL) from the n-butanol extract displayed higher radical scavenging activity than butylated hydroxytoluene. The isolated compounds were evaluated for their antiproliferative effects against human cervical adenocarcinoma (HeLa) cell line by real time cell analyzer assay and tamarixetin exhibited the remarkable effect (IC<sub>50</sub>&lt;50 μg/mL) on HeLa cells. This study supports the documented medicinal effects of <em>B. hispanica</em>.</p><p class="Abstract"><strong>Video Clip of Methodology</strong>:</p><p class="Abstract">Antiproliferative Assay: 4 min 31 sec   <a href="https://www.youtube.com/v/vi_fS47fpY0&amp;t=3s">Full Screen</a>   <a href="https://www.youtube.com/watch?v=vi_fS47fpY0&amp;t=3s">Alternate</a></p>

scholarly journals Biological activities of Annona montana Macfad extracts

2021 ◽  
Vol 26 (3) ◽  
Author(s):  
Ana Isabel Giraldo Rivera ◽  
Gloria Edith Guerrero-Álvarez
Keyword(s):  
Insecticidal Activity ◽  
Phenolic Content ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Artemia Salina ◽  
Thrips Tabaci ◽  
Total Phenolic ◽  
Promising Source

Annona montana Macfad is a fruit species of the Annonaceae family. In this study, the phytochemical potential of A. montana seeds was investigated. Ethanol and hexane extracts from seeds were evaluated for cytotoxicity and insecticidal activity, phenolic content, and antioxidant capacity. The latter being related to free radical scavenging activity assay (DPPH) and ferric reducing power (FRAP). Exposing Artemia salina to both seed extract types reveled their high toxicity with a median lethal concentration (LC50) of < 10 μg mL-1. Further A. montana seed insecticidal activity was evaluated against Thrips tabaci L., revealing that the most promising treatments were observed for a concentration of 100 mg L-1 in both extracts. The ethanol extract resulted in a mortality of 67.5 % and the hexane extract in a 53.3 % mortality. The ethanolic extract of A. montana seeds showed the highest total phenolic content: 297.38 mg GAE/100 g of dried extract and 192.66 mg TE/100 g, and 385.46 mg TE/100 g for DPPH and FRAP, respectively. The chemical characterization of both extracts by high performance liquid chromatography (HPLC) revealed the presence of acetogenins. The results obtained idicate that the A. montana extracts are a promising source of compounds with insecticidal activity.

Protective effects of ethanolic extract of Lemon Beebrush (Aloysia citrodora) leaf against hypoxia-induced lethality in mice

2020 ◽  
Author(s):  
Mohammad Hossein Hosseinzadeh ◽  
Mohammad Ali Ebrahimzadeh
Keyword(s):  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Positive Control ◽  
Experimental Models ◽  
Total Phenolic ◽  
Control Group ◽  
Antihypoxic Activity ◽  
Significant Activity ◽  
Protective Effects

Lemon Beebrush, known as Lippia citroiodora and Aloysia citrodora is a known medicinal plant in Iran. Many biological activities have been reported from this plant. In spite of many works, nothing is known about protective effect of A. citrodora against hypoxia conditions. In this study, protective effects of A. citrodora leaf extract against hypoxia-induced lethality in mice were evaluated by three experimental models of hypoxia, asphyctic, haemic and circulatory. Its phenol and flavonoid contents and antioxidant activity were also evaluated. Statistically significant protective activities were established in some doses of extract in three models. Antihypoxic activity was especially pronounced in circulatory hypoxia where extract at 62.5 mg kg-1 prolonged the latency for death with respect to control group (p<0.01). The effect was dose dependent. At 250 mg kg-1, it prolonged the latency for death with the same activity of propranolol (20 mg kg-1), that used as positive control (p>0.05). Extract showed weak activity in haemic model. Only at the highest tested dose, 250 mg kg-1, it significantly prolonged latency for death with respect to control group (p<0.05). Extract at this dose showed the same activity of propranolol which used as positive control (p>0.05). In asphytic model, extract at the highest tested dose showed statistically significant activity respect to the control. At 250 mg kg-1, it significantly prolonged the latency for death (26.84 ± 4.11 vs. 19.45 ± 1.13 min, p = 0.0006). At 125 mg kg-1, it also prolonged survival time but this increase was not significantly different. Phenytoin that used as positive control kept mice alive for 29.60 ± 2.51 min (p<0.0001). Extract at 250 mg kg-1 showed the same activity of phenytoin (p>0.05). The total phenolic content was 342.9 ± 11.5 mg gallic acid equivalent/g of extract powder and flavonoid content was 90.2 ± 7.8 mg quercetin equivalent/g of extract powder. IC50 for DPPH radical-scavenging activity was 21.97 ± 2.4 mg/ml. The presence of polyphenols in this plant may be a proposal mechanism for reported antihypoxic activities.

Sign in / Sign up

Export Citation Format

Share Document