scholarly journals Apoptotic-Like Tumor Cells and Apoptotic Neutrophils in Mitochondrion-Rich Gastric Adenocarcinomas: A Comparative Study with Light and Electron Microscopy between these Two Forms of Cell Death

Rare Tumors ◽  
2013 ◽  
Vol 5 (2) ◽  
pp. 68-71 ◽  
Author(s):  
Rosario A. Caruso ◽  
Francesco Fedele ◽  
Luciana Rigoli ◽  
Giovanni Branca ◽  
Anna Bonanno ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cell Death ◽  
Comparative Study ◽  
Tumor Cells ◽  
Light And Electron Microscopy ◽  
Gastric Adenocarcinomas

scholarly journals A Case of Iris Melanocytoma Demonstrating Diffuse Melanocytic Proliferation with Uncontrolled Intraocular Pressure

2017 ◽  
Vol 8 (1) ◽  
pp. 190-194 ◽  
Author(s):  
Mami Kusunose ◽  
Yuji Sakino ◽  
Yoshihiro Noda ◽  
Tsutomu Daa ◽  
Toshiaki Kubota
Keyword(s):  
Electron Microscopy ◽  
Intraocular Pressure ◽  
Tumor Cells ◽  
Trabecular Meshwork ◽  
Light And Electron Microscopy ◽  
Secondary Glaucoma ◽  
Pigment Dispersion ◽  
Large Tumor ◽  
History Of ◽  
Ring Melanoma

We report a rare case with histologically proven melanocytoma of the iris that demonstrated diffuse melanocytic proliferation with uncontrolled secondary glaucoma and investigate the etiology of the intraocular pressure elevation. The patient was a 78-year-old man with a history of darkened iris of his left eye. The intraocular pressure was 39 mm Hg. A slit-lamp examination showed a diffuse darkened iris, and a gonioscopic examination revealed open angle with circumferential heavy pigmentation. There was no pigment dispersion of the anterior chamber and no pigment deposition of the cornea. We suspected malignant ring melanoma in the left eye and enucleated it. The globe was examined with light and electron microscopy. Light microscopy revealed the presence of heavily pigmented tumor cells in the iris, ciliary body, trabecular meshwork, and Schlemm’s canal. A bleached preparation showed large tumor cells with central and paracentral nuclei without mitosis. Electron microscopy of the trabecular meshwork revealed melanin-bearing tumor cells invading the intertrabecular spaces, and the melanin granules were not phagocytosed in the trabecular cells. The mechanical obstruction of the aqueous flow by the tumor cells may be a major cause of secondary glaucoma in eyes with iris melanocytoma presenting diffuse proliferation.

scholarly journals Dynamics of in vivo ASC speck formation

2017 ◽  
Vol 216 (9) ◽  
pp. 2891-2909 ◽  
Author(s):  
Paola Kuri ◽  
Nicole L. Schieber ◽  
Thomas Thumberger ◽  
Joachim Wittbrodt ◽  
Yannick Schwab ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cell Death ◽  
Light And Electron Microscopy ◽  
Three Dimensional ◽  
Inflammasome Activation ◽  
Caspase Activation ◽  
Endogenous Gene ◽  
Pyrin Domain ◽  
Effector Caspase

Activated danger or pathogen sensors trigger assembly of the inflammasome adaptor ASC into specks, large signaling platforms considered hallmarks of inflammasome activation. Because a lack of in vivo tools has prevented the study of endogenous ASC dynamics, we generated a live ASC reporter through CRISPR/Cas9 tagging of the endogenous gene in zebrafish. We see strong ASC expression in the skin and other epithelia that act as barriers to insult. A toxic stimulus triggered speck formation and rapid pyroptosis in keratinocytes in vivo. Macrophages engulfed and digested that speck-containing, pyroptotic debris. A three-dimensional, ultrastructural reconstruction, based on correlative light and electron microscopy of the in vivo assembled specks revealed a compact network of highly intercrossed filaments, whereas pyrin domain (PYD) or caspase activation and recruitment domain alone formed filamentous aggregates. The effector caspase is recruited through PYD, whose overexpression induced pyroptosis but only after substantial delay. Therefore, formation of a single, compact speck and rapid cell-death induction in vivo requires a full-length ASC.

scholarly journals Comparative Study of Cultured Burkitt Tumor Cells by Immunofluorescence, Autoradiography, and Electron Microscopy

1967 ◽  
Vol 1 (4) ◽  
pp. 830-837 ◽  
Author(s):  
Harald Zur Hausen ◽  
Werner Henle ◽  
Klaus Hummeler ◽  
Volker Diehl ◽  
Gertrude Henle
Keyword(s):  
Electron Microscopy ◽  
Comparative Study ◽  
Tumor Cells

Prolactinoma of pituitary with associated amyloid-like substances

1983 ◽  
Vol 59 (6) ◽  
pp. 1067-1070 ◽  
Author(s):  
Jun-ichi Kuratsu ◽  
Yasuhiko Matsukado ◽  
Masaki Miura
Keyword(s):  
Electron Microscopy ◽  
Pituitary Adenoma ◽  
Tumor Cells ◽  
Pituitary Adenomas ◽  
Light And Electron Microscopy ◽  
Rare Entity ◽  
Short History ◽  
Content Type ◽  
History Of ◽  
Fine Print

✓ A prolactin-secreting pituitary adenoma containing amyloid substance was studied by light and electron microscopy. The tumor was found in a 32-year-old woman who presented with a short history of amenorrhea and galactorrhea. Pituitary adenoma containing amyloid substance is a very rare entity, and the implications of this association are discussed. Previous reports, suggesting that mesenchymal cells or hormone-secreting tumor cells in pituitary adenomas produce amyloid substances, are reviewed.

Mouse Hepatoblastomas: A Histologic, Ultrastructural, and Immunohistochemical Study

1988 ◽  
Vol 25 (4) ◽  
pp. 286-296 ◽  
Author(s):  
T. Nonoyama ◽  
F. Fullerton ◽  
G. Reznik ◽  
T. J. Bucci ◽  
J. M. Ward
Keyword(s):  
Electron Microscopy ◽  
Tumor Cells ◽  
Immunohistochemical Study ◽  
Light And Electron Microscopy ◽  
Alpha Fetoprotein ◽  
Cell Populations ◽  
Bile Canaliculi ◽  
Cytoplasmic Organelles ◽  
Dense Bodies ◽  
Hepatocellular Adenomas

Hepatoblastomas from B6C3F1 and BALB/c mice were examined by light and electron microscopy and by immunohistochemical reactions for alpha-fetoprotein, keratin, and vimentin. Tumors occurred in one group of a chronic bioassay for the interaction of diet, genetic strain, and the carcinogen, 2-acetylaminofluorene. Tumors had several populations (including epithelial and mesenchymal cells) in various stages of differentiation. Neoplastic epithelial cells had features of embryonal hepatocytes, such as sparse cytoplasmic organelles, absence of glycogen, abundant free ribosomes, occasional bile canaliculi, and peroxisome-like dense bodies. Embryonal fibroblast-like cells had pleomorphic and folded nuclei with prominent perinuclear chromatin and dispersed cytoplasmic organelles. Fibroblast-like cells were surrounded by bundles of collagen fibrils. Intermediate or transitional types of cells were seen. No tumor cells were immunoreactive for mouse alpha-fetoprotein (AFP) antibody, unlike those in hepatocellular adenomas or carcinomas. Epithelial and mesenchymal tumor cells contained intermediate filaments throughout the cytoplasm; some of these cells stained for keratin but not for vimentin. These findings suggest that mouse hepatoblastomas are derived from bipotential liver blastema cells and are composed of a mixture of several cell populations.

Sign in / Sign up

Export Citation Format

Share Document