scholarly journals Nilgirianthus ciliatus mediated Environment friendly extracellular synthesis of AgNps for exacting its potential against the Dengue vector, Aedes aegypti and the Microbial pathogen, Staphylococcus aureus

2018 ◽  
Vol 50 (1) ◽  
Author(s):  
K. Meenambigai ◽  
R. Kokila ◽  
A. Naresh Kumar
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Aedes Aegypti ◽  
Respiratory Infections ◽  
Food Poisoning ◽  
Bacterial Pathogen ◽  
Multidrug Resistant ◽  
Particle Dispersion ◽  
Dengue Vector ◽  
Microbial Pathogen

Aedes aegypti, the dengue vector is a menace continues since 1780’s due to development of resistant to synthetic insecticides and Staphylococcus aureus, the common microbial pathogen agent of food poisoning, skin infections and respiratory infections have developed multidrug resistant, which forced us to focus on novel agent for which the dengue vector and bacterial pathogen have not practiced to develop resistant and which cannot detoxify it using its usual enzyme activity as it did earlier. Silver nanoparticles a challenging insecticidal agents for the toxic degrading enzymes of both the insect and microbe was the target of our present study to excavate the potential in killing immature of dengue vector and bacterial pathogen. Green protocolled nanoparticles were successfully synthesized using leaf extract of Nilgirianthus ciliatus which act as reducing as well as capping agents. The UV-vis spectra observed at 441nm confirmed the presence of silver nanoparticles. XRD and FT-IR confirmed the crystalline nature and organic capping around the silver nanoparticles respectively. SEM and DLS showed the average size at 117nm and the particle dispersion was at -17.2mV zeta potential.

scholarly journals Complement Inactivation Strategy of Staphylococcus aureus Using Decay-Accelerating Factor and the Response of Infected HaCaT Cells

2021 ◽  
Vol 22 (8) ◽  
pp. 4015
Author(s):  
Kyoung Ok Jang ◽  
Youn Woo Lee ◽  
Hangeun Kim ◽  
Dae Kyun Chung
Keyword(s):  
Staphylococcus Aureus ◽  
Immune Cells ◽  
Respiratory Infections ◽  
Food Poisoning ◽  
Surface Expression ◽  
Host Cells ◽  
Hacat Cells ◽  
Decay Accelerating Factor ◽  
Ligand Expression ◽  
The Complement System

Staphylococcus aureus is a species of Gram-positive staphylococcus. It can cause sinusitis, respiratory infections, skin infections, and food poisoning. Recently, it was discovered that S. aureus infects epithelial cells, but the interaction between S. aureus and the host is not well known. In this study, we confirmed S. aureus to be internalized by HaCaT cells using the ESAT-6-like protein EsxB and amplified within the host over time by escaping host immunity. S. aureus increases the expression of decay-accelerating factor (CD55) on the surfaces of host cells, which inhibits the activation of the complement system. This mechanism makes it possible for S. aureus to survive in host cells. S. aureus, sufficiently amplified within the host, is released through the initiation of cell death. On the other hand, the infected host cells increase their surface expression of UL16 binding protein 1 to inform immune cells that they are infected and try to be eliminated. These host defense systems seem to involve the alteration of tight junctions and the induction of ligand expression to activate immune cells. Taken together, our study elucidates a novel aspect of the mechanisms of infection and immune system evasion for S. aureus.

Tackling the growing threat of dengue: Phyllanthus niruri-mediated synthesis of silver nanoparticles and their mosquitocidal properties against the dengue vector Aedes aegypti (Diptera: Culicidae)

2015 ◽  
Vol 114 (4) ◽  
pp. 1551-1562 ◽  
Author(s):  
Udaiyan Suresh ◽  
Kadarkarai Murugan ◽  
Giovanni Benelli ◽  
Marcello Nicoletti ◽  
Donald R. Barnard ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Aedes Aegypti ◽  
Dengue Vector ◽  
Phyllanthus Niruri

scholarly journals Silver Nanoparticles Synthesized by Using the Endophytic Bacterium Pantoea ananatis are Promising Antimicrobial Agents against Multidrug Resistant Bacteria

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3220 ◽  
Author(s):  
Tahmina Monowar ◽  
Md. Rahman ◽  
Subhash Bhore ◽  
Gunasunderi Raju ◽  
Kathiresan Sathasivam
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Candida Albicans ◽  
Bacillus Cereus ◽  
Antimicrobial Agents ◽  
Endophytic Bacterium ◽  
Multidrug Resistant ◽  
Pantoea Ananatis

Antibiotic resistance is one of the most important global problems currently confronting the world. Different biomedical applications of silver nanoparticles (AgNPs) have indicated them to be promising antimicrobial agents. In the present study, extracellular extract of an endophytic bacterium, Pantoea ananatis, was used for synthesis of AgNPs. The synthesized AgNPs were characterized by UV–Vis spectroscopy, FTIR, transmission electron microscopy (TEM), scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX), and Zeta potential. The antimicrobial potential of the AgNPs against pathogenic Staphylococcus aureus subsp. aureus (ATCC 11632), Bacillus cereus (ATCC 10876), Escherichia coli (ATCC 10536), Pseudomonas aeruginosa (ATCC 10145) and Candida albicans (ATCC 10231), and multidrug resistant (MDR) Streptococcus pneumoniae (ATCC 700677), Enterococcus faecium (ATCC 700221) Staphylococcus aureus (ATCC 33592) Escherichia coli (NCTC 13351) was investigated. The synthesized spherical-shaped AgNPs with a size range of 8.06 nm to 91.32 nm exhibited significant antimicrobial activity at 6 μg/disc concentration against Bacillus cereus (ATCC 10876) and Candida albicans (ATCC 10231) which were found to be resistant to conventional antibiotics. The synthesized AgNPs showed promising antibacterial efficiency at 10 µg/disc concentration against the MDR strains. The present study suggests that AgNPs synthesized by using the endophytic bacterium P. ananatis are promising antimicrobial agent.

Predatory efficiency of the copepod Megacyclops formosanus and toxic effect of the red alga Gracilaria firma-synthesized silver nanoparticles against the dengue vector Aedes aegypti

Hydrobiologia ◽  
2016 ◽  
Vol 785 (1) ◽  
pp. 359-372 ◽  
Author(s):  
Kandasamy Kalimuthu ◽  
Chellasamy Panneerselvam ◽  
Chi Chou ◽  
Showe-Mei Lin ◽  
Li-Chun Tseng ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Aedes Aegypti ◽  
Toxic Effect ◽  
Red Alga ◽  
Dengue Vector ◽  
Predatory Efficiency ◽  
Gracilaria Firma
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