scholarly journals Tetracycline Resistant Genes as Bioindicators of Water Pollution

2020 ◽  
Vol 93 (1) ◽  
Author(s):  
Manal F. Abdelall ◽  
Safa S. Hafez ◽  
Maryam El. Fayad ◽  
Hanan A. Nour El-Din, ◽  
Soad A. Abdallah
Keyword(s):  
Water Samples ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
M Gene ◽  
Chain Reaction ◽  
16S Rdna Gene ◽  
Polymerase Chain ◽  
Tet Genes ◽  
16S Sequence ◽  
Sequence Techniques

The current study aimed to investigate the prevalence of tetracycline resistant bacteria isolated from different water samples and the genes responsible for this resistance. Two hundred fifty isolates were isolated from different water samples from two different locations. Isolates were obtained from El-Zamalek site was (n =110) and from Rod El-Farag site was (n = 140). A hundred isolates out of 250 bacterial isolates (40%) were resistant to tetracycline at a concentration of 16 μg/ml. Only 31 (31%) were selected due to their resistance to (32 μg/ml) tetracycline for identification. All selected isolates were identified according to biochemical and the 16S sequence techniques. The 16S rDNA gene sequences of the bacterial isolates which were reported in this study were submitted to the NCBI database. Of the 31 isolates were analyzed by Polymerase Chain Reaction (PCR), results showed that 41.9 % (13/31) harbored tet A gene, 74.2% (23/31) carried tet D gene, while 12.9 % (4/31) carried tet M gene. Whereas tet B, tet C and tet O were not detected. Twenty-one isolates (67.7%) harbored a single tet gene, five isolates (16.1%) harbored two different tet genes while three isolates (9.7 %) harbored three different tet genes. Moreover, two isolates were free from any tested tet genes.

scholarly journals Features of Lactobacillus sakei isolated from Italian sausages: focus on strains from Ventricina del Vastese

2015 ◽  
Vol 4 (4) ◽  
Author(s):  
Carmela Amadoro ◽  
Franca Rossi ◽  
Michele Piccirilli ◽  
Giampaolo Colavita
Keyword(s):  
Meat Products ◽  
Lactobacillus Sakei ◽  
Bacterial Isolates ◽  
Chain Reaction ◽  
Spontaneous Fermentation ◽  
Meat Extract ◽  
Fermented Sausages ◽  
Polymerase Chain ◽  
High Diversity

In this study bacterial isolates from <em>Ventricina del Vastese</em> sausage, previously identified as <em>Lactobacillus (L.) sakei</em>, were characterised genotypically, physiologically and on the basis of some technologically relevant traits. A total of 70 <em>L. sakei</em> isolates from sausages manufactured with spontaneous fermentation in the same producing plant were taken into account. Six genotypic groups were distinguished on the basis of Rep-polymerase chain reaction with the GTG<sub>5</sub> primer, some of which were found only in the sausages ripened at temperatures lower than 10°C for the first two months and lower than 16°C for the remaining three months, according to the traditional ripening process. Six strains were selected as representative of the genotypic profiles and further characterised. A high diversity in their fermentation profiles was observed, and different groups were separated on the basis of growth and acidifying capacity in meat extract. None of the strains produced histamine or tyramine <em>in vitro</em>. One strain was able to slightly inhibit <em>Listeria (L.) monocytogenes</em> and <em>L. innocua</em> and all six strains were able to slightly inhibit <em>Enterobacteriaceae</em> isolated from <em>Ventricina del Vastese</em> sausages <em>in vitro</em>. Results showed that most <em>L. sakei</em> strains can have a role in improving the safety of low acidity fermented sausages, even though a limited acidifying capacity was observed in a meat-like substrate, and that <em>L. sakei</em> strains able to produce biogenic amines are unlikely to occur in spontaneously fermented meat products.

scholarly journals Diversity ofLeptospiraspp. in Rats and Environment from Urban Areas of Sarawak, Malaysia

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Chai Fung Pui ◽  
Lesley Maurice Bilung ◽  
Kasing Apun ◽  
Lela Su’ut
Keyword(s):  
Polymerase Chain Reaction ◽  
Water Samples ◽  
Urban Areas ◽  
Environmental Samples ◽  
Soil Samples ◽  
Chain Reaction ◽  
Prevalence Studies ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Soil And Water

Various prevalence studies onLeptospirain animals and humans, as well as environmental samples, had been conducted worldwide, including Malaysia. However, limited studies have been documented on the presence of pathogenic, intermediate, and saprophyticLeptospirain selected animals and environments. This study was therefore conducted to detectLeptospiraspp. in rats, soil, and water from urban areas of Sarawak using the polymerase chain reaction (PCR) method. A total of 107 rats, 292 soil samples, and 324 water samples were collected from April 2014 to February 2015. PathogenicLeptospirawas present in 5.6% (6/107) of rats, 11.6% (34/292) of soil samples, and 1.9% (6/324) of water samples. IntermediateLeptospirawas present in 2.7% (8/292) of soil samples and 1.9% (6/324) of water samples. SaprophyticLeptospirawas present in 10.3% (11/107) of rats, 1.4% (4/292) of soil samples, and 0.3% (1/324) of water samples. From this study, 76Leptospiraspp. were isolated. Based on DNA sequencing, the dominantLeptospiraspp. circulating in urban areas of Sarawak are pathogenicLeptospira noguchii, intermediateLeptospira wolffiiserovar Khorat, and saprophyticLeptospira meyeri, respectively. Overall, this study provided important surveillance data on the prevalence ofLeptospiraspp. from rats and the environment, with dominant local serovars in urban areas of Sarawak.

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