scholarly journals Study on the protective effect of an innovative cow milk-based product against some human skin-bacterial pathogens

Author(s):  
Carlo Cosentino ◽  
Hazem Salaheldin Elshafie ◽  
Cristiana Labella ◽  
Carmine D'Adamo ◽  
Giovanni Pecora ◽  
...  

There is a growing interest related to the possible use of some components of cow milk that behave as immunomodulators, exert biological activity, and have anti-inflammatory factors. The aims of the study were: i) to investigate the chemical composition and antioxidant activity of Podolian cow milk; ii) to test the efficacy of some hand soaps supplemented with different percentage of cow milk compared to placebo; iii) to evaluate the antibacterial activity of milk added to soap. Antioxidant activity of Podolian milk resulted in mean 97.03% and 52.09% utilizing 2,2’-azinobis 3-ethylbenzthiazoline-6-acid and 2,2-diphenyl-1-picrylhydrazyl methods, respectively. The efficacy test showed that the most effective soap was obtained adding 5% of Podolian milk. For this soap, total counting bacterial test evidenced the highest reduction of bacterial hand contamination assessed by 98%.

Chemija ◽  
2018 ◽  
Vol 29 (2) ◽  
Author(s):  
Kristina Ramanauskienė ◽  
Aidas Grigonis ◽  
Valdimaras Janulis ◽  
Raimondas Raudonis ◽  
Lina Babickaitė ◽  
...  

Green and red tea due to the strong antioxidant, anti-inflammatory, anti-microbial, anti-tumor features are an intensive object of research. This article explores the chemical composition and biological activity of green and red tea of different fermentation. The preparation of aqueous extracts was implemented in household conditions, and the extraction time of release of active compounds from raw material was evaluated. The antibacterial activity was evaluated by the method in vitro. The research has shown that green tea is rich in catechins and caffeine. Red tea provides rutin and gallic acid. Green tea extracts inhibited the growth of S. aureus ir B. cereus. On the basis of the research results on the antioxidant activity, teas can be arranged in the following sequence: green > white > Oolong > red.


Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2888
Author(s):  
Carmen M. S. Ambrosio ◽  
Gloria L. Diaz-Arenas ◽  
Leidy P. A. Agudelo ◽  
Elena Stashenko ◽  
Carmen J. Contreras-Castillo ◽  
...  

Essential oils (EOs) from Citrus are the main by-product of Citrus-processing industries. In addition to food/beverage and cosmetic applications, citrus EOs could also potentially be used as an alternative to antibiotics in food-producing animals. A commercial citrus EO—Brazilian Orange Terpenes (BOT)—was fractionated by vacuum fractional distillation to separate BOT into various fractions: F1, F2, F3, and F4. Next, the chemical composition and biological activities of BOT and its fractions were characterized. Results showed the three first fractions had a high relative amount of limonene (≥10.86), even higher than the whole BOT. Conversely, F4 presented a larger relative amount of BOT’s minor compounds (carvone, cis-carveol, trans-carveol, cis-p-Mentha-2,8-dien-1-ol, and trans-p-Mentha-2,8-dien-1-ol) and a very low relative amount of limonene (0.08–0.13). Antibacterial activity results showed F4 was the only fraction exhibiting this activity, which was selective and higher activity on a pathogenic bacterium (E. coli) than on a beneficial bacterium (Lactobacillus sp.). However, F4 activity was lower than BOT. Similarly, F4 displayed the highest antioxidant activity among fractions (equivalent to BOT). These results indicated that probably those minor compounds that detected in F4 would be more involved in conferring the biological activities for this fraction and consequently for the whole BOT, instead of the major compound, limonene, playing this role exclusively.


Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3741
Author(s):  
Wioleta Pietrzak ◽  
Renata Nowak

The content of plant secondary metabolites is not stable, and factors such as the region/location effect and seasonal variations have an impact on their chemical composition, especially in parasitic plants. Research in this area is an important step in the development of quality parameter standards of medicinal plants and their finished products. The effects of the time and place of harvest and the host tree species on the chemical composition and antioxidant activity of mistletoe extracts were investigated. Statistical tools were used to evaluate the results of the spectrophotometric and LC-ESI-MS/MS studies of the phenolic composition and antioxidant activity. The investigations indicate that the qualitative and quantitative composition, influencing the biological activity of mistletoe extracts, largely depends on the origin of the plant. The mistletoe extracts exhibited a rich phenol profile and high antioxidant activity. The chemometric analysis indicated that mistletoe collected from conifers (Viscum abietis and Viscum austriacum) had the most advantageous chemical composition and antioxidant activity. Moreover, the chemical profile and biological activity of the plant material were closely related to the climatic conditions and location of the harvested plant. Higher levels of phenolic compounds and high antioxidant activity were found in extracts obtained from plant material collected in cold weather with the presence of snow and less sunshine (autumn–winter period).


2020 ◽  
Vol 92 (2) ◽  
pp. 323-333
Author(s):  
Virginie Dulong ◽  
Marie-Carole Kouassi ◽  
Luc Picton

AbstractIn a previous study, we presented the development of a series of functionalized carboxymethylpullulan (CMP) grafted with aminoguaiacol (derivative of guaiacol with known antibacterial and antioxidant activities) leading to CMP-G derivatives with various degrees of substitution [DS(Ga)] from 0.16 to 0.58. Our results have shown the efficiency of the grafting both with the evidence of antioxidant and antibacterial activities (Staphylococcus aureus) of the CMP-G derivatives. Nevertheless, an important result has shown surprisingly that such biological activity was not clearly improved with the DS(Ga) unlike the antioxidant activity. These results were probably correlated with a peculiar associative behavior of the derivative (i.e. amphiphilic character) due to the grafted hydrophobic guaiacol groups leading to preferential intramolecular association which was particularly important in the more concentrated regime (polysoap behavior). To complete this study, we propose here two strategies in order to diminish the associative character and notably the polysoap behavior: (i) decrease the DS(Ga) of CMP derivative with a CMP-G0.05 [grafted with a DS(Ga) = 0.05], (ii) conduct the functionalization onto a more rigid polysaccharide backbone as alginate. Our results show a good correlation of the associative physicochemical behaviors with both antioxidant and antibacterial activities. They also confirm the availability of these strategies mainly for the first one (i.e. CMP-G0.05). The main result indicates that the lower is the DS(Ga), the better is the antibacterial activity thanks to a lower associative character. Finally, this study also shows that the grafting of aminoguaiacol is possible onto another anionic polysaccharide (i.e. alginate).


2019 ◽  
Vol 9 (3) ◽  
pp. 190-198
Author(s):  
Houda El Hajjouji ◽  
Rachid Rahhal ◽  
Said Gmouh ◽  
Mohammed Hsaine ◽  
Hassan Fougrach ◽  
...  

This study aimed to compare the chemical composition, the antioxidant activity and the antibacterial activity of essential oils (EOs) of Juniperus phoenicea, Juniperus thurifera and Juniperus oxycedrus, obtained from Ait Bouguemez region (Province of Azilal, Morocco). The analysis by GC/MS of essential oils led to identify 37, 54 and 38 components for J. phoenicea, J. thurifera, and J. oxycedrus, respectively. Monoterpenic fraction was found predominant in essential oils of the three samples. The DPPH free radical scavenging activity showed that essential oil of J. thurifera has the strongest antioxidant activity with an IC50 value of 12.07 μg/mL. The antibacterial activity showed that S. aureus was more sensitive than P. aeruginosa and E. coli for the three EOs tested.


2020 ◽  
Vol 18 (3) ◽  
pp. 224-232
Author(s):  
Behnam Mahdavi ◽  
Fereshteh Ghorat ◽  
Mahda S. Nasrollahzadeh ◽  
Mahmood Hosseyni-Tabar ◽  
Hassan Rezaei-Seresht

Introduction: In this research, we have reported the chemical composition and bioactivity of the essential oil from flowers of Matricaria chamomilla var. Chamomilla (MCCO) for the first time. The essential oil was extracted using a Clevenger-type apparatus. The chemical composition of the essential oil was identified by chromatography methods. DPPH Radical Scavenging Activity (RSA), β-carotene bleaching (BCB), and Ferrous Ion Chelating ability (FIC) were chosen to evaluate the MCCO antioxidant activity. Disc diffusion assay and Mínimum Inhibitory Concentration method (MIC) were selected to investigate antibacterial activity of MCCO. Hemolytic activity of MCCO on Red Blood Cells (RBCs) was measured through optical density. Methods: MTT method was used to determine the cytotoxicity effects of MCCO on human cáncer cells. MCCO was dominated byoxygenated sesquiterpenes (59.01%). α-Bisabolone oxide A (35.74%), α- bisabolol oxide A (19.07%), (Z)-β-farnesene (6.63%), and chamazulene (6.46%)wasfound as themajorcomponents oftheessentialoil. The oil represented an acceptable antioxidant activity. For antibacterial activity, MCCO prevented the growth of all selected microorganisms. The oil can be considered as a strong antibacterial agent as well as anantioxidant. Results: MCCO showed a low hemolytic rate (below 1.5%) on RBCs. The safety of MCCO as a food additive or other uses was suggested by the hemolysis result. Conclusions: MCCO was identified as a weak agent in the cytotoxicity assay.


2017 ◽  
Vol 60 (3) ◽  
Author(s):  
Dayane Silva Rocha ◽  
Janete Magali Da Silva ◽  
Daniela Maria Do Amaral Ferraz Navarro ◽  
Claúdio Augusto Gomes Camara ◽  
Camila Soledade De Lira ◽  
...  

The essential oils from leaves, stems and roots of Piper caldense were analyzed by GC-MS. The antibacterial potential of the oils was evaluated against gram-negative bacteria and gram-positive bacteria. The major chemical constituents that were identified from various parts of this plant were α-cardinal, α-muurolol, tujopsan-2-β-ol and δ-cadiene in the leaves, valencene, pentadecane, elina-3,7-11-dieno α-terpineol in the roots and terpine-4-ol, α-terpineol, α-cadinol 2-β-ol in the stems. Tissue oils showed antibacterial activity against the bacteria tested except for Enterococcus faecalis. This is the first report of the biological activity and chemical composition essential oil of P. caldense.


2020 ◽  
Vol 9 (2) ◽  
pp. e53921909
Author(s):  
Layanne Nascimento Fraga ◽  
Izabela Maria Montezano de Carvalho

Talisia esculenta (A. ST.-HIL.) Radlk is a Brazilian wild fruit, known as a pitomba, belonging to the Sapindaceae family. The objective of the present work was to perform a review of the literature in order to identify studies on its physico-chemical characterization, antioxidant activity and biological activity. The articles search was done in PubMed, SciELO, Science Direct and LILACS. We identified 90 publications, 8 in SciELO, 3 in LILACS, 67 in Science Direct and 12 in PubMed. Subsequently, duplication of the articles between the databases was verified, and the triage was done by reading the titles, abstracts and reading in full, to analyze which articles fit the study objective, and only 5 studies were included. The review exposes Talisia esculenta Radlk (A. ST.-HIL.) as a fruit that has a bioactive potential, but there is a shortage of studies that deal with its chemical composition and its biological activity.


2007 ◽  
Vol 2 (6) ◽  
pp. 1934578X0700200
Author(s):  
Sergio Rossellia ◽  
Antonella Maggio ◽  
Carmen Formisano ◽  
Francesco Napolitano ◽  
Felice Senatore ◽  
...  

Helleborus bocconei Ten. subsp. intermedius (Ranunculaceae) is a Sicilian medicinal plant used for the treatment of pneumonia affecting cows and horses and for the removal of human decayed molars. The goal of our study was to assess the biological activity of Helleborus bocconei subsp. intermedius by testing its extracts for their activity against bacteria known to cause respiratory diseases. The two more active extracts (light petroleum from roots and aerial parts), as well as the dichloromethane extracts, were analyzed by GC/MS and their composition is reported.


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