scholarly journals Prevalence of Mycobacterium avium subsp. paratuberculosis in milk and dairy cattle in Southern Italy: preliminary results

2013 ◽  
Vol 2 (3) ◽  
pp. 35 ◽  
Author(s):  
Giacomo Marchetti ◽  
Matteo Ricchi ◽  
Andrea Serraino ◽  
Federica Giacometti ◽  
Elena Bonfante ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Mycobacterium Avium ◽  
Southern Italy ◽  
Mycobacterium Avium Subsp ◽  
Preliminary Results ◽  
Mycobacterium Avium Subsp Paratuberculosis

scholarly journals Longitudinal Study of Mycobacterium avium Subsp. paratuberculosis Antibody Kinetics in Dairy Cattle Using Sera and Milk throughout the Lactation Period

2020 ◽  
Vol 7 (3) ◽  
pp. 81 ◽  
Author(s):  
Md. Shohel Al Faruk ◽  
Young-hoon Jung ◽  
Tai-young Hur ◽  
Sang-suk Lee ◽  
Yong-il Cho
Keyword(s):  
Dairy Cattle ◽  
Antibody Titer ◽  
Mycobacterium Avium ◽  
Elisa Test ◽  
Mycobacterium Avium Subsp ◽  
Control Group ◽  
Lactation Period ◽  
Antibody Titers ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Antibody Kinetics

Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne’s disease in dairy cattle populations around the world. The objective of this study was to evaluate MAP antibody kinetics in serum and milk samples throughout the lactation period in dairy cattle. The samples were collected simultaneously from eight MAP-positive and two healthy MAP-negative (control group) cows. The MAP antibody was detected by using serum and milk ELISA. The serum and milk MAP antibody titers fluctuated between the positive and negative cut-off values in this study. Specifically, cattle with low MAP antibody titer (<100) showed fluctuation between the cut-off values. Variable changes of MAP antibody titer were also observed after parturition. Between the serum and milk MAP antibody titers, there was a positive correlation (R2 = 0.5358) observed throughout the assessment period. The milk MAP ELISA test had low diagnostic performance in cows with low MAP titer due to its weak correlation (R2 = 0.0198). Finally, this study suggest that the periodic MAP ELISA test is recommended for the application of Johne’s eradication program due to the fluctuating nature of MAP antibody kinetics.

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