scholarly journals Analysis of lymphocyte T(CD4+) cells expression on severe early childhood caries and free caries

2020 ◽  
Author(s):  
Muhammad Luthfi ◽  
Priyawan Rachmadi ◽  
Aqsa Sjuhada Oki ◽  
Retno Indrawati ◽  
Agung Sosiawan ◽  
...  
Keyword(s):  
T Cells ◽  
Early Childhood ◽  
Early Childhood Caries ◽  
Significant Risk ◽  
Significant Risk Factor ◽  
Cell Receptor ◽  
P Value ◽  
Cd4 Cells ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

Early childhood caries (ECC) is still one of the many diseases found in children throughout the world. Cariogenic bacteria are a significant risk factor for ECC associated with early colonization and high levels of cariogenic microbes (Streptococcus mutans, S. mutans). Lymphocyte T (CD4+) cells known as helper T cells, are effector cells for mediated host immunity. Naive T cells (CD4+) must be activated to initiate effector function. This activation occurs through interaction with professional antigen- presenting cells (pro-APC), especially dendritic cells that lead to intracellular pathways that regulate T cell receptor (TCR) more specifically against antigen in T cells. Lymphocyte cells from samples were collected from severe early childhood caries (S-ECC) and Free caries aged 5 to 6 years. The subjects were instructed to gargle 10 mL of sterile NaCl 1.5% solution for 30 seconds, and expectorate it into a sterile glass then analyzing T lymphocyte cell (CD4+) expression using flow cytometry. Lymphocyte T (CD4+) cell expression at SECC (6.2525±64482) while in free caries (8.4138±1.10397) with P-value (P=0. 000). Conclusion of lymphocyte T (CD4+) cells expression at S-ECC is lower than that occurring in free caries.

scholarly journals CD89/CD35 Expression Ratio in Salivary Neutrophil as an Early Detection Marker for Severe Early Childhood Caries

2020 ◽  
Vol 14 (03) ◽  
pp. 386-392
Author(s):  
Muhammad Luthfi ◽  
Aqsa Sjuhada Oki ◽  
Retno Indrawati ◽  
Muhaimin Rifai ◽  
Yoes Prijatna Dachlan ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Early Childhood ◽  
Early Detection ◽  
Free Group ◽  
Early Childhood Caries ◽  
Kindergarten Students ◽  
P Value ◽  
Expression Ratio ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

Abstract Objectives To analyze CD35/CD89 expression ratio on the surface of neutrophils as an early detection marker for S-ECC. Materials and Methods Saliva was collected from 4- to 6-year-old kindergarten students. Salivary neutrophils were obtained by instructing the subjects to rinse their mouth with 1 mL of sterile 1.5% NaCl for 30 seconds before expectorating it into a sterile glass. The expression of CFSE+CD35+ and CFSE+CD89+was measured and analyzed using flow cytometry. Results The expression of CFSE+CD89+ in the caries-free group (2.46 ± 0.39) was significantly lower than that in the S-ECC group (3.41 ± 1.11), with a p-value of 0.0001, while the expression of CFSE+CD35+ in the caries-free group was (2.35 ± 0.56) compared with (1.54 ± 0.35) (p = 0.0001) in the S-ECC group. Conclusions The expression ratio of CFSE+CD89+ and CFSE+CD35+constitutes a marker for S-ECC.

Magnetic Bead-Based Salivary Peptidome Profiling Analysis for Severe Early Childhood Caries

2014 ◽  
Vol 49 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Yan Si ◽  
Shuang Ao ◽  
Weijian Wang ◽  
Feng Chen ◽  
Shuguo Zheng
Keyword(s):  
Mass Spectrometry ◽  
Early Childhood ◽  
Early Childhood Caries ◽  
Expression Profiles ◽  
Salivary Protein ◽  
Preschool Age ◽  
Diagnostic Model ◽  
Peptide Mass ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

Objective: To investigate the differential salivary protein expression profiles between children with severe early childhood caries (S-ECC) and caries-free (CF) children at the age of 3 years. Methods: We used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) combined with weak cation exchange magnetic beads, and peptide mass fingerprints were created by scanning mass spectrometry signals. Salivary samples from 20 children were analyzed (10 for each group). Results: Eleven protein peaks were significantly different (p < 0.05) between the two groups. Eight of these peaks were higher in the S-ECC group and three were higher in the CF group. To establish a diagnostic model for discrimination between the two groups, we chose three peptides (3,186.2, 3,195.8 and 3,324.8 Da) that exhibited the best fitted curve, by which the two groups were better separated when compared with other combinations. Conclusions: The salivary biomarkers identified revealed significant differences between the CF and the S-ECC group. Our results provide novel insight into the salivary protein profile of preschool-age children with dental caries and may lead to the development of a new strategy for screening high-risk populations.

scholarly journals Dental Treatments under the General Anesthesia in a Child with Keratitis, Ichthyosis, and Deafness Syndrome

2013 ◽  
Vol 2013 ◽  
pp. 1-6
Author(s):  
Sera Sımsek Derelioglu ◽  
Yücel Yılmaz ◽  
Sultan Keles
Keyword(s):  
Early Childhood ◽  
Case Report ◽  
General Anesthesia ◽  
Early Childhood Caries ◽  
Sensorineural Deafness ◽  
Oral Symptoms ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

KID syndrome is a rare genodermatosis characterized by keratitis, ichthyosis, and sensorineural deafness. Although the dermatological, ophthalmologic, and sensorineural defects are emphasized in the literature, oral and dental evaluations are so superficial. In this case report, dental and oral symptoms of a three year and five months old boy with KID syndrome, suffering severe Early Childhood Caries (s-ECC) and dental treatments done under General Anesthesia (GA) were reported.

scholarly journals Faktor Risiko Early Childhood Caries pada Anak UsiaPrasekolah di PAUD Kenanga 17 Kelurahan Penjaringan, Kecamatan Penjaringan, Jakarta Utara

2020 ◽  
Vol 2 (1) ◽  
pp. 012-022
Author(s):  
Anita Rosa Delima ◽  
Setya Kusuma Arum
Keyword(s):  
Early Childhood ◽  
Early Childhood Caries ◽  
Parental Education ◽  
Population Data ◽  
Total Sample ◽  
Tooth Brushing ◽  
P Value ◽  
Cross Sectional ◽  
Childhood Caries

Dental caries is a multifactorial disease that can affect all levels of society. There are several factors that take part ini caries initiation. Caries risk factors in children are related to age, gender, use of bottles, tooth brushing frequency, the role of the mother, and parental education. The purpose of this studywas to find out factors related to the cause of early childhood caries experienced by preschool children at PAUD Kenanga 17 Kelurahan Penjaringan, Kecamatan Penjaringan, North Jakarta. An observational analytical methods with cross sectional approach was used in this study. Subject in this study consisted of 20 children as a total sample of the population. Data collection was carried out using questionnaires and def-t index was employed for dental examination. Data processing and statistical analysis using chi-square test were applied with a significance value of p 0.05. The results showed significant correlation between age (p value 0.001) and gander (p value 0.047) andearly childhood caries.hawever, no correlation was observed betwen the use of bottle (p value = 0.909), frequency of tooth brushing (p value 1.00), role of mother (p value 1.00), and parental education (p value = 0.798) and early childhood caries.

Streptococcus mutans isolated from children with severe-early childhood caries form higher levels of persisters

2020 ◽  
Vol 110 ◽  
pp. 104601 ◽  
Author(s):  
Aaron Bottner ◽  
Richard Y. He ◽  
Andrea Sarbu ◽  
S.M. Hashim Nainar ◽  
Delphine Dufour ◽  
...  
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

scholarly journals Candida biome of severe early childhood caries (S-ECC) and its cariogenic virulence traits

2020 ◽  
Vol 12 (1) ◽  
pp. 1724484 ◽  
Author(s):  
Kausar Sadia Fakhruddin ◽  
Lakshman Perera Samaranayake ◽  
Hiroshi Egusa ◽  
Hien Chi Ngo ◽  
Chamila Panduwawala ◽  
...  
Keyword(s):  
Early Childhood ◽  
Early Childhood Caries ◽  
Virulence Traits ◽  
Severe Early Childhood Caries ◽  
Childhood Caries
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