scholarly journals Morphologic and immunophenotypic features of a case of acute monoblastic leukemia with unusual positivity for Glycophorin-A

2018 ◽  
Vol 10 (4) ◽  
Author(s):  
Giovanni Carulli ◽  
Paola Sammuri ◽  
Cristiana Domenichini ◽  
Martina Rousseau ◽  
Virginia Ottaviano ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Essential Role ◽  
Leukemic Cells ◽  
Specific Marker ◽  
Glycophorin A ◽  
Leukemic Transformation ◽  
Erythroid Precursor ◽  
Acute Monoblastic Leukemia ◽  
Acetate Esterase ◽  
Antibody Panel

Acute monoblastic leukemia (AMoL) is characterized by cells with highly undifferentiated morphology. Cytochemistry with non-specific esterases is negative in up to 20% of cases. Immunophenotyping by flow cytometry has an essential role in diagnosing such a subtype of leukemia and a multiparametric approach with a wide monoclonal antibody panel is necessary. We describe a case of AMoL with morphology resembling either plasma blasts or very immature erythroblasts. Diagnosis was made by alpha-naphtyl-acetate esterase staining and with immunophenotyping, which was made with a wide monoclonal antibody panel. Blasts were positive for monocytic markers. Most of leukemic cells, however, were positive for Glycophorin-A. The presence of Glycophorin-A, which is considered as a specific marker of the erythroid lineage, has never been reported previously in cases of AMoL. This peculiar immunophenotype might be interpreted as deriving from a common myelo-erythroid precursor undergone leukemic transformation.

scholarly journals Phenotype study of fresh and cultured hairy cells with the use of immunologic markers and electron microscopy

Blood ◽  
1984 ◽  
Vol 64 (2) ◽  
pp. 547-552
Author(s):  
M Divine ◽  
JP Farcet ◽  
MF Gourdin ◽  
A Tabilio ◽  
A Vasconcelos ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
Peroxidase Activity ◽  
Leukemic Cells ◽  
Specific Marker ◽  
Surface Immunoglobulin ◽  
Immunologic Markers ◽  
Hairy Cells

The phenotype of fresh and cultured leukemic cells from patients with hairy cell leukemia was studied using a panel of monoclonal antibodies in addition to the detection of peroxidase activity under electron microscopy. In fresh samples, the leukemic cells from 11 patients displayed predominantly a B phenotype, as judged by their reactivity with the B1 monoclonal antibody and surface immunoglobulin expression. Ultrastructural peroxidase activity, characteristic of hairy cells, was observed in all cases studied. When hairy cells were cultured in the presence of phytohemagglutinin and irradiated T cells, their phenotype converted from surface Ig+, B1+, OKT3-, OKT11- to surface Ig-, B1+, OKT3-, OKT11+. In contrast, the peroxidase activity remained unchanged. Some hairy cells were also OKM1+, but no conclusion could be made about the MO2 antigen, a more specific marker of monocytes. The variability of the phenotype in vivo and in vitro indicates that reliable markers are required for identifying hairy cells. When studied together, the staining by B1 monoclonal antibody and the ultrastructural detection of peroxidase, enable the identification of hairy cells with certainty.

scholarly journals Phenotype study of fresh and cultured hairy cells with the use of immunologic markers and electron microscopy

Blood ◽  
1984 ◽  
Vol 64 (2) ◽  
pp. 547-552 ◽  
Author(s):  
M Divine ◽  
JP Farcet ◽  
MF Gourdin ◽  
A Tabilio ◽  
A Vasconcelos ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
Peroxidase Activity ◽  
Leukemic Cells ◽  
Specific Marker ◽  
Surface Immunoglobulin ◽  
Immunologic Markers ◽  
Hairy Cells

Abstract The phenotype of fresh and cultured leukemic cells from patients with hairy cell leukemia was studied using a panel of monoclonal antibodies in addition to the detection of peroxidase activity under electron microscopy. In fresh samples, the leukemic cells from 11 patients displayed predominantly a B phenotype, as judged by their reactivity with the B1 monoclonal antibody and surface immunoglobulin expression. Ultrastructural peroxidase activity, characteristic of hairy cells, was observed in all cases studied. When hairy cells were cultured in the presence of phytohemagglutinin and irradiated T cells, their phenotype converted from surface Ig+, B1+, OKT3-, OKT11- to surface Ig-, B1+, OKT3-, OKT11+. In contrast, the peroxidase activity remained unchanged. Some hairy cells were also OKM1+, but no conclusion could be made about the MO2 antigen, a more specific marker of monocytes. The variability of the phenotype in vivo and in vitro indicates that reliable markers are required for identifying hairy cells. When studied together, the staining by B1 monoclonal antibody and the ultrastructural detection of peroxidase, enable the identification of hairy cells with certainty.

A monoclonal antibody that detects expression of transferrin receptor in human erythroid precursor cells

Blood ◽  
1982 ◽  
Vol 59 (3) ◽  
pp. 671-678 ◽  
Author(s):  
D Lebman ◽  
M Trucco ◽  
L Bottero ◽  
B Lange ◽  
S Pessano ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Cell Line ◽  
Cell Lines ◽  
Peripheral Blood ◽  
Transferrin Receptor ◽  
Time Course ◽  
Surface Membrane ◽  
Peripheral Blood Lymphocytes ◽  
Leukemic Cells ◽  
Erythroid Precursor

Abstract A monoclonal antibody, L5.1, obtained by immunizing a Balb/c mouse with HL60 human promyelocytic leukemia cells, was found to react with both HL60 cells and with the K562(S) cell line. This monoclonal antibody binds and immunoprecipitates a glycoprotein (Mr 87,000) present on the cell surface membrane of K562(S) as a disulfide bonded dimer. In competition experiments L5.1 competes with both transferrin and OKT9 (a known antitransferrin receptor antibody) for binding to target K562(S) erythroleukemia cells. Binding of both L5.1 and transferrin to the surface of K562(S) cells is inhibited by treatment with 12--O- tetradecanoyl-phorbol-13-acetate, and the extent and time course of inhibition is similar in both cases. Cell sorting analysis of normal human marrow cells incubated with L5.1 indicates that L5.1 reacts strongly with all the morphologically recognizable erythroid lineage precursors, from the pronormoblast to the orthochromatic normoblast, and with reticulocytes. Erythrocytes, myeloid elements, monocytes, megakaryocytes and platelets, peripheral blood B and T lymphocytes do not bind significantly with this antibody and only a small fraction of promyelocytes was reactive. Antibody L5.1 did not react with leukemic cells of patients with acute lymphoblastic, myeloblastic and promyelocytic leukemias, but it did react with some established B (1 of 5) and T (2 of 3) cell lines, and a myeloid (1 of 3) cell line, and with PHA-stimulated peripheral blood lymphocytes. The nonhemopoietic cell lines tested did not bind with L5.1 with the exception of a colorectal adenocarcinoma and a melanoma cell line, which were both strongly positive. The relationship of antibody L5.1 to other monoclonal antibodies that bind the transferrin receptor is discussed.

scholarly journals A monoclonal antibody that detects expression of transferrin receptor in human erythroid precursor cells

Blood ◽  
1982 ◽  
Vol 59 (3) ◽  
pp. 671-678
Author(s):  
D Lebman ◽  
M Trucco ◽  
L Bottero ◽  
B Lange ◽  
S Pessano ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Cell Line ◽  
Cell Lines ◽  
Peripheral Blood ◽  
Transferrin Receptor ◽  
Time Course ◽  
Surface Membrane ◽  
Peripheral Blood Lymphocytes ◽  
Leukemic Cells ◽  
Erythroid Precursor

A monoclonal antibody, L5.1, obtained by immunizing a Balb/c mouse with HL60 human promyelocytic leukemia cells, was found to react with both HL60 cells and with the K562(S) cell line. This monoclonal antibody binds and immunoprecipitates a glycoprotein (Mr 87,000) present on the cell surface membrane of K562(S) as a disulfide bonded dimer. In competition experiments L5.1 competes with both transferrin and OKT9 (a known antitransferrin receptor antibody) for binding to target K562(S) erythroleukemia cells. Binding of both L5.1 and transferrin to the surface of K562(S) cells is inhibited by treatment with 12--O- tetradecanoyl-phorbol-13-acetate, and the extent and time course of inhibition is similar in both cases. Cell sorting analysis of normal human marrow cells incubated with L5.1 indicates that L5.1 reacts strongly with all the morphologically recognizable erythroid lineage precursors, from the pronormoblast to the orthochromatic normoblast, and with reticulocytes. Erythrocytes, myeloid elements, monocytes, megakaryocytes and platelets, peripheral blood B and T lymphocytes do not bind significantly with this antibody and only a small fraction of promyelocytes was reactive. Antibody L5.1 did not react with leukemic cells of patients with acute lymphoblastic, myeloblastic and promyelocytic leukemias, but it did react with some established B (1 of 5) and T (2 of 3) cell lines, and a myeloid (1 of 3) cell line, and with PHA-stimulated peripheral blood lymphocytes. The nonhemopoietic cell lines tested did not bind with L5.1 with the exception of a colorectal adenocarcinoma and a melanoma cell line, which were both strongly positive. The relationship of antibody L5.1 to other monoclonal antibodies that bind the transferrin receptor is discussed.

Novel use of the BD FACS™ SPA to automate custom monoclonal antibody panel preparations for immunophenotyping

2005 ◽  
Vol 66B (1) ◽  
pp. 40-45 ◽  
Author(s):  
Abigail S. Kelliher ◽  
David W. Parent ◽  
David C. Anderson ◽  
Michelle E. Dorn ◽  
Jessica L. Hahn ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Antibody Panel

Monocytic Leukemia in a Horse

1984 ◽  
Vol 21 (4) ◽  
pp. 394-398 ◽  
Author(s):  
E. Burkhardt ◽  
F. v. Saldern ◽  
B. Huskamp
Keyword(s):  
Electron Microscopy ◽  
Lymph Nodes ◽  
Light And Electron Microscopy ◽  
Leukemic Cells ◽  
Monocytic Leukemia ◽  
Impaired Performance ◽  
History Of ◽  
Acetate Esterase ◽  
Generalized Lymphadenopathy ◽  
Naphthyl Acetate

On clinical examination, a six-year-old Hassian gray gelding with a history of impaired performance, slight cough, colic, and edema of the ventral abdomen, prepuce and the legs had reduced skin turgor, pale mucous membranes, forced costoabdominal breathing, reduced venous return, enlarged lymph nodes, and splenomegaly. Hematologic findings revealed anemia, leukocytosis and a high percentage of monocytoid leukemic cells. Generalized lymphadenopathy, splenomegaly, ascites, hydrothorax, and a diffusely thickened gut wall were found at necropsy. Massive infiltration with monocytoid leukemic cells was detected in lymph nodes, spleen, bone marrow, liver, gut wall, kidneys, and choroid plexus. Incubation of living cells obtained from a leukocyte concentrate with latex particles revealed phagocytosis in the leukemic cells on light and electron microscopy. The leukemic cells also had a marked α-naphthyl-acetate and naphthol-AS-acetate esterase activity, but were only weakly positive to naphthol-AS-D-chloroacetate esterase. A very weak alkaline phosphatase activity only was demonstrated in a few leukemic cells. On scanning electron microscopy, the leukemic cells had prominent ruffles and ridge-like profiles. These features of the leukemic cells excluded lymphocytic and granulocytic leukemia, and monocytic leukemia was diagnosed.

scholarly journals A monoclonal antibody reacting with human basophils

Blood ◽  
1987 ◽  
Vol 69 (5) ◽  
pp. 1414-1418
Author(s):  
MP Bodger ◽  
GL Mounsey ◽  
J Nelson ◽  
PH Fitzgerald
Keyword(s):  
Monoclonal Antibody ◽  
Cell Line ◽  
Myeloid Leukemia ◽  
Leukemia Cell ◽  
Leukemic Cells ◽  
Leukemia Cell Line ◽  
Hel Cells ◽  
Basophilic Leukemia Cell ◽  
Human Basophils ◽  
Basophilic Leukemia

Bsp-1 is an IgM murine monoclonal antibody raised against the human erythroblastic leukemia cell line (HEL) that reacts with basophils but not neutrophils or eosinophils. Western blotting techniques showed that Bsp-1 reacts with a 45-kilodalton surface antigen on HEL cells. The distribution of Bsp-1 antigen on leukemic cells is confined to a basophilic leukemia cell line, KU812, chronic myeloid leukemia with basophilia, and some cases of acute undifferentiated leukemia. Bsp-1 might therefore be a useful reagent for the study of basophil function and differentiation.

A novel monoclonal antibody induces the differentiation of monocyte leukemic cells

1990 ◽  
Vol 168 (3) ◽  
pp. 898-904 ◽  
Author(s):  
Yoshikazu Koyama ◽  
Banri Yamanoha ◽  
Takeshi Yoshida
Keyword(s):  
Monoclonal Antibody ◽  
Leukemic Cells

Reticulated Platelets: Changing Focus from Basics to Outcomes

2018 ◽  
Vol 118 (09) ◽  
pp. 1517-1527 ◽  
Author(s):  
Bashar Hannawi ◽  
Yousef Hannawi ◽  
Neal Kleiman
Keyword(s):  
Coronary Artery Disease ◽  
Coronary Artery ◽  
Essential Role ◽  
State Of The Art ◽  
Specific Marker ◽  
Messenger Ribonucleic Acid ◽  
Coronary Syndrome ◽  
Reticulated Platelets ◽  
Artery Disease ◽  
Circulating Levels

AbstractPlatelets play an essential role in the pathophysiology of atherothrombosis. Reticulated platelets (RPs) are the youngest platelet population in the circulation; their presence is an indicator of platelet turnover. Circulating levels of RPs are increased in patients with coronary artery disease and stroke. Preliminary indications are that the proportion of circulating RP is associated with the likelihood of ischaemic events such as acute coronary syndrome and stroke. Plausible mechanisms include: (1) increased participation of these platelets in thrombosis due to messenger ribonucleic acid that may be translated to active proteins, (2) lack of exposure to anti-platelet drugs since they are newly released from the bone marrow or (3) their presence is a non-specific marker of inflammation. In this state-of-the-art review, we discuss the implication of RP in coronary artery disease and in hypo-responsiveness to the most commonly used anti-platelet drugs.

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