scholarly journals Wound healing effects of Persian walnut (Juglans regia L.) green husk on the incision wound model in rats

2020 ◽  
Vol 30 (1) ◽  
pp. 210-218
Author(s):  
Ayat Taheri ◽  
Sayid Mahdi Mirghazanfari ◽  
Masoumeh Dadpay
Keyword(s):  
Nitric Oxide ◽  
Wound Healing ◽  
Phenolic Content ◽  
Aqueous Ethanol ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Persian Walnut ◽  
Incision Wound

Walnut green husk (WGH) has been mentioned as a wound-healing agent in traditional Iranian medicine. Although previous studies indicated that WGH is a good source of pharmaceutical ingredients, they did not assess its wound healing activity; so the present study set out the scientific validation of the wound healing potential of the Persian walnut. Total phenolic content, reducing power, DPPH, and nitric oxide scavenging activity of aqueous ethanol extract of WGH was evaluated. Forty-eight male Wistar albino rats were divided into four groups of 12 each. An incision wound was created on the dorsal region of each rat. WGH extract (20% w/w), WGH burnt residues (20% w/w), Eucerin, and Phenytoin ointments were used in each group. Wound length, contraction percentage, and histopathological evaluations were recorded on days 3, 7, 10, and 14. Total phenolic content and EC50 values of reducing power, DPPH and nitric oxide scavenging activity of the WGH extract were 61.34 ± 0.64 mg/g dry extract, 0.95 ± 0.02 mg/mL, 0.35 ± 0.01 mg/mL, and 0.28 ± 0.01 mg/mL, respectively. Treated animals with WGH extract showed significantly (p ≤ 0.05) better results for physical and pathological parameters compared to the control group; overall, WGH extract showed better results than WGH burnt residues. The present study indicated that the WGH aqueous ethanol extract has a promising potential for wound healing in the animal model and could be a valuable resource for developing new wound-healing medicines for humans.

scholarly journals Effects of solvent extraction system on antioxidant activity of Lamium purpureum L.

2017 ◽  
Vol 71 (5) ◽  
pp. 361-370
Author(s):  
Slavica Grujic ◽  
Ana Dzamic ◽  
Violeta Mitic ◽  
Vesna Stankov-Jovanovic ◽  
P.D. Marin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Radical Scavenger ◽  
Scavenging Activity

Antioxidant and free radical scavenging activity of methanol, ethanol, ethyl acetate and chloroform extracts of aerial parts of Lamium purpureum L. was determined by DPPH, ABTS, FRAP and TRP assays. Contents of flavonoids and phenols were also investigated. The total phenolic content in the extracts, determined using Folin?Ciocalteu assay, ranged between 8.57 to 128.00 mg GAE/g d.e. while concentrations of flavonoids in the extracts varied from 24.20 to 39.80 mg QuE/g d.e. The highest phenolic content was found in methanol extract (128.00 mg GAE/g d.e.). The highest content of total flavonoids was identified in the methanol extract (39.80 mg QuE/g d.e.) and the lowest was in the chloroform (24.30 mg QuE/g d.e.). DPPH scavenging of the extracts was determined and obtained IC50 values ranged from 0.12 to 3.12 mg/mL of solution. The values of ABTS radical scavenging activity ranged from 0.35 to 1.80 mg AA/g. The highest ABTS antiradical activity was registered for methanol extract. The FRAP value was found within the range 0.08 to 1.04 ?mol Fe/mg. The best radical scavenger was methanol (1.04 ?mol Fe/mg). In reducing power assay different extracts of L. purpureum showed increasing of activity with increased concentration, and all extracts possessed substantial dose dependent antioxidant activity. The best reducing capacity was obtained with methanol extract of L. purpureum (0.0132 mg AA/mL). The results in this study confirmed that L. purpureum possesses moderate antioxidant properties.

scholarly journals In-vitro antioxidant potential of the flavonoid glycosides from Cassia tora linn. Leaves

2016 ◽  
Vol 3 (1) ◽  
pp. 19-26
Author(s):  
A. Vijayalakshmi ◽  
S. Jayakumari ◽  
K. Masilamani
Keyword(s):  
Nitric Oxide ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanol Extract ◽  
Antioxidant Potential ◽  
Flavonoid Glycosides ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Cassia Tora ◽  
Ic50 Values

The present study aimed to study the anti-oxidant potential of isolated flavonoids from the ethanol extract (70%v/v) of Cassia tora leaves. Three flavonoids were isolated viz Luteolin-7-O-β-glucopyranoside (I), quercetin-3-O-β-d-glucuronide (II) and Formononetin-7-O-β-D-Glucoside (III) from the ethanol extract (70%v/v) of Cassia tora leaves in the previous study. A comprehensive study on the phytochemical contents - total phenolic content and flavonoid content was accessed using Folin-Denis and AlCl3 method respectively. The antioxidant potential of the samples was evaluated using inhibition of hydroxyl radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitric oxide scavenging methods. The total phenolic content and flavonoid content was found to be 18.60 % w/w and 9.5% w/w respectively. The IC50 values of ethanol extract against hydroxyl, DPPH and nitric oxide radical were found to be 270 µg/mL, 190 µg/mL and 130 µg/mL respectively. The radical scavenging activity of the isolated flavonoids decreased in the following order: quercetin (IC50 values 15, 14, 18 µg/mL) >formononetin (IC50 values 19, 21, 14 µg/mL) > luteolin (IC50 values 20, 23, 18 µg/mL) respectively.

scholarly journals Antioxidant, Anti-Inflammatory and Antibacterial Activities of the Seeds of A Sri Lankan Variety of Carica Papaya

2019 ◽  
Vol 12 (2) ◽  
pp. 539-547
Author(s):  
Akshani Anjula Wijesooriya ◽  
Srianthie A. Deraniyagala ◽  
Chamari M. Hettiarachchi
Keyword(s):  
Ascorbic Acid ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Sri Lankan ◽  
Scavenging Activity ◽  
Flavonoid Content ◽  
Anti Inflammatory

Plant based remedies are of much importance in healthcare due to low side effects. The effective constituents contained in seeds of a papaya have not been utilized efficiently in the production of medicines. The purpose of this study was driven towards determining the total phenolic content, total flavonoid content, antioxidant capacity, anti-inflammatory activity and antibacterial properties of the aqueous seeds extract of a Sri Lankan variety (Red Lady) of papaya (AESP). The AESP prepared according to the method of “Kasaya” in Ayurvedic medicine was used for the investigations according to standard procedures. Total phenolic content and the total flavonoid content of the AESP were 13.5±2.2 mg (pyrogallol equivalence)/g and 315.9±104.6 mg (quercetin equivalence)/g respectively. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of AESP ranged from 12.4% to 81.2% for concentrations of 1.0-20.0 mg/ml; ascorbic acid gave 31.7% - 91.9% activity. The AESP displayed strong reducing power compared to ascorbic acid in the reducing power assay. The hydroxyl radical scavenging activity of the AESP ranged from 6.6% to 69.1% for concentrations ranging from 20.0-95.0 mg/ml; ascorbic acid activity was 37.7%-74.9%. The nitric oxide radical scavenging activity of AESP was 23.9%-62.7% for concentrations of 2.0-20.0 µg/ml; ascorbic acid gave 26.8%-63.5% activity. AESP concentrations ranging from 75.0-150.0 µg/ml exhibited human red blood cell (HRBC) membrane stabilization protection of 15.5%-22.7% compared to 50.8%-58.4% for aspirin. The AESP showed antibacterial activity against Bacillus subtilis and Staphylococcus aureus bacterial species. AESP possess promising antioxidant, anti-inflammatory and antibacterial activity. The aqueous decoction of the discarded seeds of papaya would facilitate remedies for many diseases in which radicals are implicated as well as assist against certain bacterial infections and also has an anti-inflammatory potential.

scholarly journals Feasibility of Using Bitterness and Foaming Properties as Surrogate Instrument in Assessing Antioxidant Potentials of Selected Eggplant Cultivars

2019 ◽  
Vol 4 (2) ◽  
Author(s):  
Eunice M Ogunbusola ◽  
Bode Daramola
Keyword(s):  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Health Promoting ◽  
Foaming Capacity ◽  
Antioxidant Markers ◽  
Insight Into

This study attempt to circumvent the hurdle associated with the utilization of laborious and expensive procedure to assess some health promoting properties of eggplant fruits. The feasibility of using simple and cheap procedure as a surrogate tool to gain insight into the antioxidant potentials of eggplant cultivars was evaluated. Some eggplants cultivars obtained fresh were used for the work. Extracts from the garden eggs were analysed for relative reducing power, total phenolic content and radical scavenging activity using DPPH. Physical and Sensory attributes such as foaming ability and stability, and level of bitterness were also carried out. Results showed that assessment of some antioxidant markers notably total phenolic content (2.266 – 6.406), radical scavenging activity (35.00 – 82.50) and relative reducing power (0.139 – 1.145), showed positive correlation (r = 0.60281 - 0.803348; r = 0.861877 -0.905333; and r = 0.851459 - 0.958941) with bitterness, foaming capacity and foaming stability respectively. Simple and cheap quality control protocol could be used as surrogate tool to gain insight into the antioxidant markers, a health promoting endowment of some eggplants. Keywords - Eggplants, bitterness, foaming capacity, surrogate tool, antioxidant potentials.

scholarly journals Effect of grinding and extraction conditions on the determination of antioxidant activity and phenolic acids in barley

2021 ◽  
Author(s):  
Bing Zhou ◽  
Zhao Jin ◽  
Paul B. Schwarz ◽  
Yin Li
Keyword(s):  
Phenolic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Dpph Scavenging

AbstractIn the present study, the objective was to evaluate the effects of barley kernel size, grind level, and extract solvent on the antioxidant activities associated with total phenolic content and phenolic acid compositions. Three barley varieties (Kindred, Azure, and Tradition), were used and the results showed that with the exception of superoxide anion radical scavenging activity, thin kernel size fraction (2.0 mm) showed much higher antioxidant activities and total phenolic content than those in the 2.4 mm and 2.8 mm fractions when averaged across barley cultivar, grind level, and extract solvent. A similar trend was found for individual phenolic acid compositions among kernel sizes. Simple correlation analysis revealed that total phenolic content showed strong correlation with DPPH· scavenging activity, ABTS + scavenging activity, and reducing power. Generally, fine grind and 80% acetone extract showed averaged highest values in antioxidant activities, total phenolic content, and individual phenolic acid compositions. Stepwise linear regression showed that extract solvent was the most important factor for DPPH· scavenging activity, ABTS + scavenging activity, reducing power, and iron chelating activity. The sum of syringic acid and caffeic acid exhibited a dominant role in explaining the major variation in antioxidant activities except for superoxide anion radical scavenging activity.

scholarly journals Propolis Extract as Antioxidant to Improve Oxidative Stability of Fresh Patties during Refrigerated Storage

Foods ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 614 ◽  
Author(s):  
Rey Vargas-Sánchez ◽  
Gastón Torrescano-Urrutia ◽  
Brisa Torres-Martínez ◽  
Mirian Pateiro ◽  
José Lorenzo ◽  
...  
Keyword(s):  
Oxidative Stability ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Butylated Hydroxytoluene ◽  
Total Phenolic ◽  
Propolis Extract ◽  
Pork Patties

The effect of propolis ethanol extract (PEE), butylated hydroxytoluene (BHT), and ascorbic acid (Asc) against lipid (Lox) and protein oxidation (Pox), color deterioration, and the antioxidant stabilizer of raw beef and pork patties during chilled storage (9 days at 2 °C/under darkness) was investigated. Total phenolic content (TPC), reducing power ability (RPA), DPPH● radical scavenging activity (FRSA) of the PEE was evaluated. Meat samples were evaluated for pH, Lox (TBARS), Pox (Carbonyls), color (L*, a*, b*, C*, and h*), metmyoglobin formation (MMb), TPC, RPA, and FRSA. Results indicated that PEE is rich in phenolic content and antioxidant activity, and their incorporation in beef and pork patties reduced (p < 0.05) Lox and Pox (TBARS-88.7 and 80% inhibition; Pox-47.3 and 30.6% inhibition, respectively), as well as loss of color and increased the oxidative stability throughout storage.

scholarly journals Various antioxidant assays of agarwood extracts (Gyrinops versteegii) from West Lombok, West Nusa Tenggara, Indonesia

2019 ◽  
Vol 3 (01) ◽  
pp. 1-5 ◽  
Author(s):  
AMALIA INDAH PRIHANTINI ◽  
KANTI DEWI RIZQIANI
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Dry Extract ◽  
Antioxidant Assays ◽  
Β Carotene

Abstract. Prihantini AI, Rizqiani K. 2019. Various antioxidant assays of agarwood extracts (Gyrinops versteegii) from West Lombok, West Nusa Tenggara, Indonesia. Asian J Agric 3: 1-5. However, the species have not been widely explored as a source of natural products in particular antioxidant agents, which protect cells from damage caused by free radicals. The present study was aimed to evaluate antioxidant activities of agarwood extracts from West Nusa Tenggara using various antioxidant assays. The antioxidant activity of leaf, fruit and fruit bark extracts were investigated based on DPPH radicals scavenging activity, reducing power, and β-carotene bleaching assays. The total phenolic content was also investigated. The result showed that leaf extract revealed the strongest antioxidant activity on all assays performed such as DPPH radicals scavenging activity (IC50 22.13±0.71 μg/mL); reducing power (251.85±0.03 mg QE/g dry extract); and β-carotene bleaching activity (IC50 24.23±2.60 μg/mL). The total phenolic content (TPC) in the leaf was higher (184.90±0.76 mg GAE/g dry extract) than fruit bark and bark extracts. The high content of phenolic compounds in G. versteegii leaves indicated that these compounds might contribute to the antioxidant activities. In conclusion, these findings showed that G. versteegii leaves are potential for development as an antioxidant source.

scholarly journals Scavenging Activity, Anti-Inflammatory and Diabetes Related Enzyme Inhibition Properties of Leaves Extract from some Varieties of Phoenyx dactylifera l.

2013 ◽  
Vol 14 ◽  
pp. 125-135 ◽  
Author(s):  
Laouini Salah Eddine ◽  
Ladjel Segni ◽  
Gherraf Noureddine ◽  
Ouahrani Mohammed Redha ◽  
Mokni Sonia
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Significant Inhibition ◽  
Total Phenolic ◽  
No Production ◽  
Scavenging Activity ◽  
Anti Inflammatory

In this study we investigate the antioxidant, anti-inflammatory, and antidiabetic activities of ethanolic leaves extracts of three selected varieties of Phoenyx dactylifera L. namely: “Ghars”, “Deglet Nour” and “Hamraya”. The assessment of the antioxidant potential of crude leaves extracts, using superoxide anions inhibition, DPPH and total antioxidant activity essays, was carried out. Furthermore, the anti-inflammatory properties of the extracts were determined by measuring the inhibition of nitric oxide (NO) production. Moreover, the antidiabetic effect was evaluated by inhibition of α-amylase enzymes. The total phenolic content measured by Folin-Ciocalteu method was as well conducted. The raw leaves extracts of the selected varieties was found to contain a high content of total phenolic content (342.45 mg GAE/gDW for GE) and therefore exhibited a higher antioxidant activity and inhibitory effect of radicals scavenging activity against DPPH and superoxide anion (IC50 = 7.44 μg/mL and 39.11 μg/mL respectively). The three varieties exhibited significant inhibition nitric oxide using In Vitro assay (IC50 = 240.28 μg/mL for GE). The extracts also displayed high inhibition actions against α-amylase enzymes. The results suggest that the leaves of the three selected varieties of Phoenyx dactylifera can be considered as a good source of natural antioxidant and anti-inflammation drugs as well as potent antidiabetic medicine.

Antioxidant Potential of Ecklonia cavaon Reactive Oxygen Species Scavenging, Metal Chelating, Reducing Power and Lipid Peroxidation Inhibition

2006 ◽  
Vol 12 (1) ◽  
pp. 27-38 ◽  
Author(s):  
Mahinda Senevirathne ◽  
Soo-Hyun Kim ◽  
Nalin Siriwardhana ◽  
Jin-Hwan Ha ◽  
Ki-Wan Lee ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Lipid Peroxidation ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Reducing Power ◽  
Total Phenolic ◽  
Oxygen Species ◽  
Metal Chelating ◽  
Lipid Peroxidation Inhibition

The antioxidative potential of different fractions (respective organic and aqueous fractions of n-hexane, chloroform and ethyl acetate) of 70% methanol extract of Ecklonia cava(a brown seaweed) was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion, hydrogen peroxide, hydroxyl radical, nitric oxide, ferrous ion chelating, reducing power and lipid peroxidation inhibition (conjugated diene hydroperoxide and thiobarbituric acid-reactive substances production) assays. The 70% methanol extract showed significant (p< 0.05) activities in all antioxidant assays and contained a high level of total phenolic content. It was observed that the level of hydrophilic phenolic content was higher than that of hydrophobics. Among those organic solvent fractions, ethyl acetate fraction exhibited significant activities due to the highest level of total phenolic content and their IC50 values were 0.013mg/mL, 0.009mg/mL and 0.33mg/mL in DPPH, hydrogen peroxide and nitric oxide radical inhibition, respectively. These activities were superior to those of a commercial synthetic and natural antioxidants tested. The aqueous chloroform and ethyl acetate fractions also exhibited significant (p< 0.05) activities in reactive oxygen species (ROS) scavenging and metal chelating, attributed to the high amount of hydrophilic phenolics. Moreover, E. cava extracts showed strong reducing power and a notable capacity to suppress lipid peroxidation.

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