scholarly journals Aberrant DKK3 expression in the oral leukoplakia and oral submucous fibrosis: a comparative immunohistochemical study

2016 ◽  
Author(s):  
T. Al-dhohorah ◽  
M. Mashrah ◽  
Z. Yao ◽  
J. Huang
Keyword(s):  
Histological Grade ◽  
Oral Submucous Fibrosis ◽  
Suppressor Gene ◽  
Oral Leukoplakia ◽  
Premalignant Lesions ◽  
Severe Dysplasia ◽  
Normal Oral Mucosa ◽  
Significant Difference ◽  
Risk Of Progression ◽  
Submucous Fibrosis

We aimed to assess and compare the expression of Dickkopf homolog 3 (DKK3), a possible tumor suppressor gene (TSG), in oral leukoplakia (OLK) and oral submucous fibrosis (OSF) using immunohistochemistry. Seventy-five cases of normal oral mucosa (NOM), OLK, OSF, and squamous cell carcinoma (OSCC) were studied. DKK3 was expressed in all cases of NOM, OLK and OSCC. There was steady increases in the percentage of the positive cells progressing toward OSCC. The expression was localized in the cytoplasm and cell membrane of cell affected by OLK with mild dysplasia and OLK with severe dysplasia. No significant association was observed between DKK3 expression and dysplastic status of OLK. Loss of DKK3 expression was observed in 15 of 30 cases in the OSF group, which was significantly associated with histological grade of OSF (P<0.0001). The percentage of positive cells gradually declined with the increasing severity of epithelial atrophy. A significant difference (P<0.01) was observed when comparing DKK3 expression among different groups of OLK and OSF cases. DKK3 may have diverse expressions in oral premalignant lesions. Loss of DKK3 expression in dysplastic/advanced stage of OSF may imply a high risk of progression to oral cancer.

Nuclear Blebbing Frequency in Tobacco-Induced Oral Potentially Malignant Disorders: A Pilot Study

2021 ◽  
pp. 1-8
Author(s):  
Roopa Yadahalli ◽  
Supriya Kheur ◽  
Aanchal Adwani ◽  
Ramesh Bhonde ◽  
A. Thirumal Raj ◽  
...  
Keyword(s):  
Oral Mucosa ◽  
Tobacco Use ◽  
Oral Submucous Fibrosis ◽  
Oral Leukoplakia ◽  
Clinical Staging ◽  
Oral Potentially Malignant Disorders ◽  
Normal Oral Mucosa ◽  
Significant Difference ◽  
Potentially Malignant Disorders ◽  
Potentially Malignant

<b><i>Introduction:</i></b> Tobacco contains several genotoxic agents including N-nitrosamine which has the potential to cause significant nuclear damage. Nuclear blebbing is a form of protrusion on the nuclear membrane and could potentially be caused by tobacco-induced genotoxicity and is closely associated with malignancy. Thus, the present study aimed to assess if tobacco-associated oral potentially malignant disorders including oral submucous fibrosis (OSF) and oral leukoplakia have a higher nuclear blebbing frequency than patients with normal oral mucosa with no history of tobacco use. <b><i>Methods:</i></b> The sample consisted of patients with OSF (<i>n</i> = 30) and oral leukoplakia (<i>n</i> = 10) and normal oral mucosa (<i>n</i> = 10). Exfoliated cells collected from the study groups were smeared on a clean microscopic slide and stained by May-Grunwald-Giemsa stain. A baseline frequency of nuclear blebbing was evaluated using a bright-field microscope with a ×100 objective. The number of nuclear blebbing per 1,000 epithelial cells was recorded and expressed in percentage. ANOVA, the Mann-Whitney U test, and Spearman’s correlation were used to analyze the data. <b><i>Results:</i></b> The mean rank of distribution of nuclear blebbing showed significant difference between all 3 groups, with the highest frequency noted in leukoplakia, followed by oral submucous and normal oral mucosa. Within OSF, the frequency of nuclear blebbing significantly increased from early stage to advanced stage. In OSF, a statistically significant positive linear correlation was noted between duration (in years), frequency (per day) of tobacco use, clinical grading, and nuclear blebbing. <b><i>Discussion/Conclusions:</i></b> The frequency of nuclear blebbing was significantly higher in oral potentially malignant disorders than normal mucosa. Nuclear blebbing also exhibited a strong dose- and time-dependent correlation with tobacco usage and clinical staging in OSF. The nuclear blebbing frequency could be a noninvasive, economic tool to assess malignant risk in tobacco-induced oral potentially malignant disorders.

scholarly journals Comparative Study of Intralesional Dexamethasone Plus Hyaluronidase & Oral Colchicine in Patients with Oral Submucous Fibrosis

2018 ◽  
Vol 14 (2) ◽  
pp. 60-65
Author(s):  
Ganesh Prasad Neupane ◽  
Maya Rai ◽  
R. S. Rathore ◽  
V. K. Bhargava ◽  
A. K. Mahat ◽  
...  
Keyword(s):  
Treatment Outcomes ◽  
Oral Mucosa ◽  
Oral Submucous Fibrosis ◽  
Mouth Opening ◽  
Study Groups ◽  
T Test ◽  
Significant Difference ◽  
Intralesional Injections ◽  
Submucous Fibrosis ◽  
Larger Sample

Introduction: Oral Submucous Fibrosis (OSMF) is a precancerous condition of the oral mucosa. It is characterized by excessive production of collagen leading to inelasticity of the oral mucosa and atrophic changes of the epithelium.Aim and objective: To evaluate the efficacy of oral Colchicine in comparison to intralesional injections of Dexamethasone plus Hyaluronidase in the management of OSMF patients.Materials and Methods: Fourty patients with OSMF were randomly divided equally into two groups. 20 patients in Dexamethasone group received biweekly intralesional injections of Dexamethasone (4mg/ml) plus Hyaluronidase 1500 IU in buccal mucosa for a period of 12 weeks. Other 20 patients in Colchicine group received oral Colchicine 0.5 mg tablets twice daily for 12 weeks. Parameters taken in the study were burning sensation, and mouth opening. Descriptive statistics, paired t test and unpaired t test were used for statistical analysis.Results and Conclusions: The pre- and post-treatment differences were found to be statistically significant for both the groups (p<0.001) and for both the treatment outcomes. When the average difference of the treatment outcomes was compared between the two study groups, statistically highly significant difference was noted (p <0.001) only in mouth opening but not in burning sensation.These encouraging results should prompt further clinical trials with Colchicine on a larger sample size to broaden the therapeutic usefulness of the drug in the management of OSMF. JNGMC,  Vol. 14 No. 2 December 2016, Page: 60-65

scholarly journals Regulation of Collagen Synthesis in Tobacco/Pan-Masala Induced Oral Sub-Mucosal Fibrosis and Diagnostic Approach - Review Article

2021 ◽  
pp. 134-143
Author(s):  
Shraddha Awasthi ◽  
Ausaf Ahmad ◽  
Anand Narain Srivastava
Keyword(s):  
Oral Mucosa ◽  
Collagen Synthesis ◽  
Oral Submucous Fibrosis ◽  
Malignant Lesion ◽  
Life Quality ◽  
Premalignant Lesions ◽  
Reference List ◽  
Transformation Rate ◽  
Dental Services ◽  
Submucous Fibrosis

<p><strong>Introduction :</strong> oral submucosal fibrosis (OSMF) is related to juxta epithelial inflammatory response followed by a fibroelastic change of the lamina propria, with epithelial atrophy leading to stiffness of the oral mucosa, causing trismus and inability to eat.</p> <p><strong>Methods and Materials:</strong> The suitability standards for studies were: Research Articles that studied the metabolic activities in oral submucous fibrosis mainly caused by some carcinogenic substances or chemicals which may present in tobacco, alcohol, cigarettes, pan-masala etc Reference list of selected articles and reviews on the topic also were searched. studies were extracted, A total of 90-100 studies were extracted, using the keywords oral submucous fibrosis, oral potentially malignant lesion and diagnosis, collagen synthesis etc.,</p> <p><strong>Results: </strong>cancer risk among these patients, periodic biopsies of suspicious regions of the oral mucosa are essential for the early detection and management of high?risk oral premalignant lesions and prevention of cancer.</p> <p><strong>Conclusion</strong>: OSMF is hardly recognized and is poorly understood across the globe. significant improvement in management, nor reduction in its high malignant transformation rate. Better integration of medical and dental services, especially in developing countries, may reduce patients’ suffering and improve their life quality.</p>

Carcinogenesis of Male Oral Submucous Fibrosis Alters Salivary Microbiomes

2020 ◽  
pp. 002203452096875
Author(s):  
M.Y. Chen ◽  
J.W. Chen ◽  
L.W. Wu ◽  
K.C. Huang ◽  
J.Y. Chen ◽  
...  
Keyword(s):  
Malignant Transformation ◽  
Oral Submucous Fibrosis ◽  
Oral Microbiome ◽  
Premalignant Lesions ◽  
Rrna Gene ◽  
Transformation Rate ◽  
Validation Data ◽  
Oral Carcinogenesis ◽  
Key Species ◽  
Submucous Fibrosis

Most oral squamous cell carcinoma (OSCC) tumors arise from oral premalignant lesions. Oral submucous fibrosis (OSF), usually occurring in male chewers of betel quid, is a premalignant stromal disease characterized by a high malignant transformation rate and high prevalence. Although a relationship between the inhabited microbiome and carcinogenesis has been proposed, no detailed information regarding the oral microbiome of patients with OSF exists; the changes of the salivary microbiome during cancer formation remain unclear. This study compared the salivary microbiomes of male patients with OSCC and a predisposing OSF background (OSCC-OSF group) and those with OSF only (OSF group). The results of high-throughput sequencing of the bacterial 16S rRNA gene indicated that OSF-related carcinogenesis and smoking status significantly contributed to phylogenetic composition variations in the salivary microbiome, leading to considerable reductions in species richness and phylogenetic diversity. The microbiome profile of OSF-related malignancy was associated with increased microbial stochastic fluctuation, which dominated the salivary microbiome assembly and caused species co-occurrence network collapse. Artificial intelligence selection algorithms consistently identified 5 key species in the OSCC-OSF group: Porphyromonas catoniae, Prevotella multisaccharivorax, Prevotella sp. HMT-300, Mitsuokella sp. HMT-131, and Treponema sp. HMT-927. Robust accuracy in predicting oral carcinogenesis was obtained with our exploratory and validation data sets. In functional analysis, the microbiome of the OSCC-OSF group had greater potential for S-adenosyl-l-methionine and norspermidine synthesis but lower potential for l-ornithine and pyrimidine deoxyribonucleotide synthesis and formaldehyde metabolism. These findings indicated that the salivary microbiome plays important roles in modulating microbial metabolites during oral carcinogenesis. In conclusion, our results provided new insights into salivary microbiome alterations during the malignant transformation of OSF.

scholarly journals Use of buccal fat pad flap for premalignant buccal mucosa defect reconstruction

2020 ◽  
Vol 7 (11) ◽  
pp. 3769
Author(s):  
Pankaj Kshirsagar ◽  
Prabhat B. Nichkaode ◽  
Saicharan Reddy ◽  
Manichandra Reddy C.
Keyword(s):  
Buccal Mucosa ◽  
Postoperative Morbidity ◽  
Oral Submucous Fibrosis ◽  
Premalignant Lesions ◽  
Fast Method ◽  
Fat Pad ◽  
Medical College ◽  
Operative Complications ◽  
Submucous Fibrosis ◽  
Buccal Pad Of Fat

Premalignant lesions over buccal mucosa are very common in India, some of these lesions require excision leaving behind defects and various techniques can be used in reconstruction of these defects. Small palatal defect, oroantral fistulas have been successfully closed by buccal pad of fat flap. Aim of this study was to evaluate reconstruction of defects after excision of premalignant lesion over buccal mucosa using buccal pad of fat. 20 patients were selected for this study who presented with different premalignant lesions like erythroplakia, proliferative verrucous leukoplakia, chronic hyperplastic candidiasis, Oral submucous fibrosis over buccal mucosa in Dr. DY Patil medical college surgery unit 4 and 7. Excision of lesion followed by reconstruction with buccal pad of fat was done. Patients were followed up every 14 days for 14 weeks, and was evaluated for epithelisation of flap and post-operative complications like infection and flap necrosis and also the functionality of the flap. In this study we have observed complete epithelisation of the flap in most of our patients. Dehiscence of the wound was observed in 3 of the patients with large defects (>4×5 cm). No postoperative morbidity was in postoperative patients. Buccal pad of fat is an excellent technique for reconstruction of small defects of buccal mucosa. It is a reliable and a fast method which has easy accessibility less complications and minimal morbidity.

Loss of Fhit Expression in Squamous Cell Carcinoma and Premalignant Lesions of the Larynx

2005 ◽  
Vol 114 (2) ◽  
pp. 127-131 ◽  
Author(s):  
Tadashi Yuge ◽  
Junji Shibahara ◽  
Kenichi Nibu ◽  
Niro Tayama ◽  
Kenji Kondo ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Suppressor Gene ◽  
Squamous Cell Carcinomas ◽  
Premalignant Lesions ◽  
Complex Method ◽  
Biopsy Specimens ◽  
Significant Difference ◽  
Fhit Protein

The tumor suppressor gene FHIT (fragile histidine triad) at chromosomal position 3p14.2 is altered by deletions in human tumors. The frequency and specificity of its inactivation vary among carcinomas, but few articles have referred to premalignant lesions such as dysplasia. We studied the expression of FHIT in a series of squamous cell carcinomas and premalignant lesions of the larynx. We observed 36 laryngeal carcinoma biopsy specimens and 70 dysplasia biopsy specimens. We studied FHIT expression in carcinoma and dysplasia with the immunohistochemical ABC (avidin–biotinylated peroxidase complex) method. Loss of FHIT protein was observed in 42% of the squamous cell carcinomas and 23% of the premalignant lesions. There was no significant difference among the three grades of dysplasia in FHIT expression. These findings of loss of FHIT protein expression, not only in squamous cell carcinoma, but also in premalignant lesions, indicate that FHIT alterations play an important role in the early events of carcinogenesis.

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