scholarly journals Establishment and biological characteristics of a Jingning chicken embryonic fibroblast bank

2011 ◽  
Vol 55 (1) ◽  
pp. 4 ◽  
Author(s):  
C. Bai ◽  
D. Wang ◽  
C. Li ◽  
D. Jin ◽  
C. Li ◽  
...  
Keyword(s):  
Biological Characteristics ◽  
Chicken Embryonic Fibroblast ◽  
Embryonic Fibroblast

Prokaryotic Expression and Anti-IBDV Activity of Chicken Interleukin-18 and Interferon-γ

2017 ◽  
Vol 153 (1) ◽  
pp. 36-45 ◽  
Author(s):  
Baifen Song ◽  
Xiaoting Li ◽  
Jinzhu Ma ◽  
Liquan Yu ◽  
Zhenyue Feng ◽  
...  
Keyword(s):  
Natural Killer Cells ◽  
Natural Killer ◽  
Prokaryotic Expression ◽  
Interferon Γ ◽  
Fibroblast Cells ◽  
Killer Cells ◽  
Chicken Embryonic Fibroblast ◽  
Prokaryotic Expression Vector ◽  
Ifn Γ ◽  
Embryonic Fibroblast

Interferon-γ (IFN-γ), a cytokine produced by activated natural killer cells and T lymphocytes, is an important regulator of innate and adaptive immunity. Interleukin (IL)-18, also known as IFN-γ-inducing factor, is a cytokine that induces T and natural killer cells to produce IFN-γ. In this study, the chicken IL-18 (ChIL-18) and chicken IFN-γ (ChIFN-γ) genes were inserted into the pET28a prokaryotic expression vector, resulting in pET28a-IL-18 and pET28a-IFN-γ, respectively. These plasmids were transformed into Escherichia coli strain BL21, and the ChIL-18 and ChIFN-γ proteins were expressed and purified. To determine their antiviral activities, 200 ng/mL of ChIL-18 and/or ChIFN-γ were inoculated into chicken embryonic fibroblast cells. After 24 h, one 50% tissue culture infective dose (TCID50) of infectious bursal disease virus (IBDV) was inoculated into the chicken embryonic fibroblast cells. The results showed that the antiviral effect of ChIL-18 and ChIFN-γ in combination was better than that of ChIL-18 or ChIFN-γ alone. Next, 14-day-old chicken were injected with 200 µg of ChIL-18 and/or ChIFN-γ and then were challenged with 103 TCID50 of IBDV via intraperitoneal injection. The results showed that the proliferation of IBDV was inhibited by the injection of the recombinant proteins, especially the combination of ChIL-18 and ChIFN-γ, as evidenced by cytokine detection, quantitative PCR, and pathology analyses. These results indicate that ChIL-18 and ChIFN-γ could inhibit IBDV infection and the combination of ChIL-18 and ChIFN-γ has a better inhibitory effect than either cytokine alone.

Induced Pluripotency in Chicken Embryonic Fibroblast Results in a Germ Cell Fate

2014 ◽  
Vol 23 (15) ◽  
pp. 1755-1764 ◽  
Author(s):  
Yangqing Lu ◽  
Franklin D. West ◽  
Brian J. Jordan ◽  
Erin T. Jordan ◽  
Rachel C. West ◽  
...  
Keyword(s):  
Germ Cell ◽  
Cell Fate ◽  
Induced Pluripotency ◽  
Chicken Embryonic Fibroblast ◽  
Embryonic Fibroblast

scholarly journals Molecular characterization of chicken syndecan-2 proteoglycan

2002 ◽  
Vol 366 (2) ◽  
pp. 481-490 ◽  
Author(s):  
Ligong CHEN ◽  
John R. COUCHMAN ◽  
Jacqueline SMITH ◽  
Anne WOODS
Keyword(s):  
Xenopus Laevis ◽  
Transmembrane Domain ◽  
Heparan Sulphate ◽  
Fibroblast Cell ◽  
In Vitro Transcription ◽  
Northern Analysis ◽  
Nucleotide Polymorphisms ◽  
Chicken Embryonic Fibroblast ◽  
Embryonic Fibroblast

A partial syndecan-2 sequence (147bp) was obtained from chicken embryonic fibroblast poly(A)+ RNA by reverse transcription–PCR. This partial sequence was used to produce a 5′-end-labelled probe. A chicken liver cDNA library was screened with this probe, and overlapping clones were obtained encompassing the entire cDNA of 3kb. The open reading frame encodes a protein of 201 amino acids. The cytoplasmic domain is identical with that of mammalian syndecan-2, and highly similar to those of Xenopus laevis and zebrafish syndecan-2. The transmembrane domain is identical with that of mammalian and zebrafish syndecan-2, and highly similar to that of Xenopus laevis syndecan-2. The ectodomain is 45–62% identical with that of zebrafish, Xenopus laevis and mammalian syndecan-2. Two coding single nucleotide polymorphisms were observed. In vitro transcription and translation yielded a product of 30kDa. Western blotting of chicken embryonic fibroblast cell lysates with species-specific monoclonal antibody mAb 8.1 showed that chicken syndecan-2 is substituted with heparan sulphate, and that the major form of chicken syndecan-2 isolated from chicken fibroblasts is consistent with the formation of SDS-resistant dimers, which is common for syndecans. A 5′-end-labelled probe hybridized to two mRNA species in chicken embryonic fibroblasts, while Northern analysis with poly(A)+ RNAs from different tissues of chicken embryos showed wide and distinct distributions of chicken syndecan-2 during embryonic development. This pattern was different from that reported for syndecan-4, but consistent with the roles of syndecan-2 in neural maturation and in mesenchymal–matrix interactions.

scholarly journals Characterization of Endogenous Avian Leukosis Viruses in Chicken Embryonic Fibroblast Substrates Used in Production of Measles and Mumps Vaccines

2001 ◽  
Vol 75 (8) ◽  
pp. 3605-3612 ◽  
Author(s):  
Jeffrey A. Johnson ◽  
Walid Heneine
Keyword(s):  
Rt Pcr ◽  
Rna Sequences ◽  
Virus Particles ◽  
Chicken Embryonic Fibroblast ◽  
Full Length Sequence ◽  
Avian Leukosis Viruses ◽  
Low Levels ◽  
Culture Supernatants ◽  
Embryonic Fibroblast

ABSTRACT Previous findings of low levels of reverse transcriptase (RT) activity in chick cell-derived measles and mumps vaccines showed this activity to be associated with virus particles containing RNA of both subgroup E endogenous avian leukosis viruses (ALV-E) and endogenous avian viruses (EAV). These particles originate from chicken embryonic fibroblast (CEF) substrates used for propagating vaccine strains. To better characterize vaccine-associated ALV-E, we examined the endogenous ALV proviruses (ev loci) present in a White Leghorn CEF substrate pool by restriction fragment length polymorphism. Five ev loci were detected, ev-1, ev-3, ev-6, ev-18, andev-19. Both ev-18 andev-19 can express infectious ALV-E, while ev-1, ev-3, and ev-6 are defective. We analyzed the full-length sequence of ev-1 and identified an adenosine insertion within the pol RT-β region at position 5026, which results in a truncated RT-β and integrase. We defined the 1,692-bp deletion in the gag-pol region ofev-3, and we found that in ev-6, sequences from the 5′ long terminal repeat to the 5′ pol region were absent. Based on the sequences of the ev loci, RT-PCR assays were developed to examine expression of ALV-E particles (EV) in CEF supernatants. Both ev-1- and ev-3-like RNA sequences were identified, as well as two other RNA sequences with intact pol regions, presumably of ev-18 andev-19 origin. Inoculation of susceptible quail fibroblasts with CEF culture supernatants from both 5-azacytidine-induced and noninduced CEF led to ALV infection, confirming the presence of infectious ALV-E. Our data demonstrate that both defective and nondefective ev loci can be present in CEF vaccine substrates and suggest that both ev classes may contribute to the ALV present in vaccines.

scholarly journals Changes in the NS1 gene of avian influenza viruses isolated in Thailand affect expression of type I interferon in primary chicken embryonic fibroblast cells

2013 ◽  
Vol 24 (3) ◽  
pp. 365-372 ◽  
Author(s):  
Chutamas Thepmalee ◽  
Phanchana Sanguansermsri ◽  
Naratchala Suwanankhon ◽  
Chanpen Chamnanpood ◽  
Pornchai Chamnanpood ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Type I Interferon ◽  
Influenza Viruses ◽  
Type I ◽  
Fibroblast Cells ◽  
Avian Influenza Viruses ◽  
Chicken Embryonic Fibroblast ◽  
Affect Expression ◽  
Ns1 Gene ◽  
Embryonic Fibroblast
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