scholarly journals A comparative study of Chicago Sky Blue and ParkerTM ink blue black potassium hidroxide in the diagnosis of dermatophytes

2019 ◽  
Author(s):  
Netty Sukmawati ◽  
R. Rahmadewi ◽  
Evy Ervianti

Parker ink blue black potassium hydroxide (KOH) is a regular stain for the diagnosis of dermatophytoses. KOH giving less color contrast to the fungal element. The Chicago Sky Blue (CSB) solution is a new contrast have various sensitivity and specificity values. This is analytical observational,cross sectional design study conducted at Dermatomycology Division of Outpatient Clinic, Dr. Soetomo General Hospital. 40 samples from lesion and perilesional infected area, diagnosed dermatophytoses with culture positive was include in this study. All two tests were performed examined by KOH and CSB. The slides were examined after incubation in x 10 and x 40 magnifications by experienced observer. The sensitivity, specificity and McNemar test of CSB and KOH was calculated using culture as the standard test. The sensitivities, specificities, PPV, NPV of the Chicago sky blue and KOH respectively are 95% and 100%, 95,24% and 100%, 100% and 100%, with McNemar test p = 1 and Kappa score is 0,950. The Chicago Sky Blue stain provides a good color contrast and IT could be a alternative staining for the dermatophytoses.

2020 ◽  
Vol 16 (2) ◽  
pp. 155
Author(s):  
Amalia Nadiasari ◽  
Diana Tri Ratnasari ◽  
Masfufatun Masfufatun

Abstract: Superficial dermatomycosis is a skin, nail and hair infection caused by fungal pathogen. Based on the pathogen, this infection can be divided into dermatophytosis, pityriasis versicolor and superficial candidiasis. The rapid and proper diagnosis is necessary to determine the initial theraphy and prevent the treatment delay. Superficial dermatomycosis diagnosis can be performed using anamnesis, physical examination or supporting investigation. The routine investigation method commonly use Potassium Hydroxide (KOH) because the KOH method is easy to be performed, rapid, simple and affordable. Chicago Sky Blue (CSB) is a dye to give a better color contrast to the fungi so the fungi would be easier to be detected. Objectives of this research is to observe the sensitivity and specifity difference of Chicago Sky Blue (CSB) dye and Potassium Hydroxide (KOH) methods for Superficial Dermatomycosis. The research was performed using cross sectional design analitical obsevation with 30 research subjects. The subjects consist of 15 superficial dermatomycosis patients and 15 non-superficial dermatomycosis patients. The samples were taken from the patients lesion swabs. The samples were checked using KOH and CSB, then observed by the medical analyst. The superficial dermatomycosis samples consist of mostly dermathophytosis (53.33%), then pityriasis versicolor (26.67%) and superficial candidiasis (20%). The sensitivity and specificity of KOH were 86.67% and 100%, respectively. The sensitivity and specificity of CSB were 93.33% and 100%, respectively. The CSB dye method has a higher sensitivity than KOH. The fungal elements are nicely dyed and more easily detected using CSB dye.  Keywords: KOH, Chicago Sky Blue, sensitivity, specificity, superficial dermatomycosis


2017 ◽  
pp. 64-71
Author(s):  
Vera Yulia ◽  
Mefri Yanni ◽  
Didik Hariyanto

Background: Determining severity of mitral regurgitation (MR) is very important, asit is related with prognosis and management of the disease. Currently, there is no goldstandard exists for quantification of MR severity using echocardiography. AmericanSociety of Echocardiography (ASE) guidelines recommend several parameters basedon integrative criteria using color and spectral Doppler and anatomic measurement.Left Ventricular Early Inflow Outflow Index (LVEIO Index) was proposed as one parameterto assess the severity of MR by omitting geometric error from regurgitantvolume method. Thus, LVEIO index offers a simple, easy and accurate measurementin determining severity of MR.Methods: This study was a diagnostic test research with cross-sectional design. Thesubjects were all patients with organic mitral regurgitation who underwent echocardiographyexamination to measure severity of MR using the LVEIO index as well asother parameters measurements based on parameters of ASE guidelines. Diagnostictest was used to determine the sensitivity, specificity, positive predictive value, negativepredictive value and accuracy of LVEIO index.Results: Of 49 patients in this study, 23 of them have severe MR and 26 are non-severeMR patients. The proportion of gender between two groups almost similar with an agerange from 56 to 61 years and the most common etiology finding was degenerative MR.Echocardiography examination showed the ejection fraction in severe MR was 63±4.2%and 64±3.9% in non-severe MR. The value of LAVI, MPAP, E-wave, VC, EROA andLVEIO index increased parallel with severity of MR (LAVI; 67±18.2 vs. 40±14.2 ml/m2,MPAP; 28±12.8 vs. 20±12.6 mmHg, E-wave 1.5±0.3 vs. 1±0.2 m/s, VC 0.8±0.3 vs.0.5±0.1 cm, EROA 0.4±0.1 vs 0,2±0,1cm2, LVEIO 9±2,4 vs. 5±1,8). The sensitivity,specificity, and accuracy of LVEIO index for diagnosis of severe MR were 86%, 84%,and 89%, respectively.Conclusion: LVEIO can be proposed as a relatively simple, easy and accurate methodin determining severity of MR.


2020 ◽  
Vol 9 (2) ◽  
pp. 218
Author(s):  
Firda Ulfa Ramadhani ◽  
Diana Tri Ratnasari Ratnasari ◽  
Masfufatun Masfufatun

Superficial dermatomycosis is a fungal infection of the skin, nail and hair. Based on the pathogen, this infection can be divided into dermatophytosis, phytiriasis versicolor and candidiasis. The rapid diagnosis for this infection is the key point to deliver the right theraphy. The supporting investigation for this infection can be performed directly using KOH. The KOH 20% solution has an indistict color contrast, that cause the fungal element seemed transparent. The observant expertise would be required for this case. The alternative method to enhance sensitivity and specificity of KOH is to add dyes such as Blue Black parker ink. The objectives of this research was to observe the sensitivity and specificity of KOH 20% + blue black parker ink in comparisson with KOH 20% method in superficial dermatomycosis. The research method was cross sectional design analitical obsevation with 30 research subjects. The samples were taken from superficial dermatomycosis lesion swabs. These samples were tested using KOH 20% solution and KOH 20% + blue black parker ink solution in Siti Khodijah Muhammadiyah Hospital, Sepanjang and dr. Makmuri Clinics, Surabaya. The results demonstrated that the sensitivity of KOH 20% solution and KOH 20% + blue black parker ink solution were 86.67% and 93.33%, respectively. The specificity of KOH 20% solution and KOH 20% + blue black parker ink solution were 100% and 100%, respectively. It can be concluded from the research that KOH 20% + blue black parker ink solution can be used for the routine diagnosis for superficial dermatomycosis due to the high sensitivity and specificity.


2021 ◽  
Vol 8 (1) ◽  
pp. 053-059
Author(s):  
Indrastuti Normahayu ◽  
Whenny Pramusinta ◽  
Widanto ◽  
Sri Andarini ◽  
Yuyun Yueniwati

Introduction: Intussusception is a pediatric emergency. If it is not treated immediately, the obstruction process can cause arterial obstruction and leads to intestinal necrosis. Not all patient shows classic syndrome triad. Thus, imaging is needed to make the diagnosis. Ultrasonography is the first choice of imaging in children because it is easy, without radiation and sedation. It has high accuracy for the diagnosis of intussusception. Ultrasonographic features of intussusception including target or doughnut sign and pseudo kidney sign. The presence of trapped fluid in intussusception, correlated with intestinal ischemia and necrosis found in surgery. This research aimed to assess sensitivity, specificity, and accuracy of trapped fluid in ultrasound examination compared with the intra-operative findings reported by surgeons in the form of intestinal necrosis in pediatric patients with intussusception in Saiful Anwar Hospital, Malang. Material and Methods: This research was observational analytic with a cross-sectional design. It used retrospective data of abdominal ultrasound results and surgical operating reports in 30 patients who were diagnosed with intussusception. Results: Trapped fluid depiction on ultrasound has a sensitivity of 80%, a specificity of 75%, and an accuracy of 76% with regards to necrotic bowel as compared to intra-operative findings. Conclusion: Trapped fluid in intussusception is a good predictor of intestinal necrosis in cases of intussusception in children.


2021 ◽  
Vol 5 (2) ◽  
Author(s):  
Heriyanto Lim ◽  
Made Sukmawati ◽  
Wayan Dharma Artana ◽  
Made Kardana ◽  
Putu Junara Putra

Sepsis is a major problem in neonatal care that has not been resolved. This condition is becoming one of major cause of morbidity and mortality in neonates especially in developing countries. Neutrophil lymphocytes count ratio (NLCR) is a new marker of infection that is easy, rapid method and less expensive that can be done in limited health care facilities. A retrospective study with cross sectional design was conducted on 84 neonates with sepsis. The data was collected from medical records from April 2018 to September 2018 in Perinatology Division Sanglah hospital. ROC curve is was done to determine the value cut-off point, sensitivity and specificity of NLCR. With cut-off point of 2.31 NLCR has prevalence, sensitivity, specificity, positive predictive value (PPV) and negative pedictive value (NPV) of 26.1%, 81.8%, 66.1%, 46.1% and 91.1%. NLCR is potential to be used in the diagnosis of neonatal sepsis.


2020 ◽  
Author(s):  
Aprezo Pardodi Maba ◽  
Mulawarman ◽  
Ma'rifatin Indah Kholili ◽  
Anugrah Intan Cahyani ◽  
kushendar ◽  
...  

Background: This study was conducted based on many studies on mental health problems during the Covid-19 pandemic and the urgency for the availability of mental health services. Unfortunately, research related to help-seeking intention for mental health in general public, particularly in Indonesia, is very limited. Meanwhile, several studies have shown that help-seeking intention is a good predictor whether someone will attend mental health services or not.Objective: The aims of this study are to determine the sources of help that are most soughtafter and the factors predicting help-seeking intention during the COVID-19 pandemic.Methods: Online instruments consist of demographical questions, 11 items of General Helpseeking Questionnaire (GHSQ) (Wilson, Deane, Ciarrochi, & Rickwood, 2005), 7 items of Fear of COVID-19 Scale (FCV-19S) (Ahorsu et al., 2020), and 5 items of Coronavirus Anxiety Scale (CAS) (Lee, 2020) were distributed via email, Facebook messenger, and Whatsapp during August 11 – 21, 2020. The data were analyzed using multiple linear regression and multivariate logistic regression.Results: The results showed that parents were the most sought-after source of help during the COVID-19 pandemic. While age, education, occupation, living in infected area, and fear significantly predict help-seeking intention. Meanwhile, the factors significantly predict the level of help-seeking intention are anxiety and fear.Limitations: This research uses a cross-sectional design. Therefore, it cannot test help-seeking intention after data collection. Furthermore, data was obtained using the self-report method which depends on the participants' ability to understand the items of the questionnaire. Conclusion: The results showed that parents were the main choice of the participants if they seek for help. Therefore, it is important for parents to be equipped with skills in providing mental health help for their children or relatives. Moreover, all parties should be equipped and trained to provide mental health help. Mental health services should be provided to various demographical background of general public during of COVID-19 pandemic. The attention of mental health services should be directed to the not infected area as well rather than infected area only.


2020 ◽  
Vol 80 (04) ◽  
pp. 268-279
Author(s):  
Johana Santa María ◽  
◽  
Mariela Zavaleta

Objective: To assess the performance of the protein/creatinine index to predict proteinuria significantly and secondarily, to find the best cut-off point that had better sensitivity and specificity. Methods: Diagnostic test study developed in a cross-sectional design of patients exposed to the hypertensive syndrome of pregnancy who did or did not develop posterior preeclampsia. 173 patients were selected non-probabilistically. The new cutoff of protein/creatinine index was found using the ROC curve, we calculated sensitivity, specificity, positive and negative prediction value, positive and negative likelihood ratios and find the Spearman correlation with 24 hours urine proteinuria. Data analysis was performed using STATA software version 13. Results: The new cutoff was 0.39 with 68.3% sensitivity, specificity 73.5%, positive prediction value 91.3%, negative prediction value 36.2%, likelihood ratios (+) 2.58, likelihood ratios (-) 0.43 and an area under the curve of 0.7799. Excellent correlation was obtained with 24 hours proteinuria (rs =0.9308, p=0.000). Conclusion: Although the new cutoff for the protein/creatinine index is different from conventional, this conventional this is more specific and therefore may be useful in the outpatient management on patients with low suspicious of preeclampsia in our population. Keywords: Preeclampsia, Protein/Creatinine index, 24 hours proteinuria.


Crisis ◽  
2020 ◽  
pp. 1-7
Author(s):  
Laura R. Umphrey ◽  
John C. Sherblom ◽  
Paulina Swiatkowski

Abstract. Background: Cultivating positive feelings of self in relationships with others can affect perceptions of belongingness and burdensomeness. Aims: The present study examines the relationships of self-compassion, hope, and emotional control to thwarted belongingness, perceived burdensomeness, and suicidal ideation. Method: Participants were 481 college students who completed scales measuring self-compassion, hope, emotional control, thwarted belongingness, perceived burdensomeness, and suicidal ideation. Results: Correlation and parallel mediation analysis results show relationships between self-compassion, hope, and emotional control with perceived burdensomeness, thwarted belongingness, and suicidal ideation. Limitations: The study is limited by its cross-sectional design, sample demographics, and inability to distinguish between individuals with suicidal ideation and those who attempt suicide. Conclusion: The results show that the relationships of self-compassion, hope, and emotional control to perceived burdensomeness, thwarted belongingness, and suicidal ideation are worth further investigation.


Author(s):  
Caspar C. Berghout ◽  
Jolien Zevalkink ◽  
Abraham N. J. Pieters ◽  
Gregory J. Meyer

In this study we used a quasiexperimental, cross-sectional design with six cohorts differing in phase of treatment (pretreatment, posttreatment, 2-year posttreatment) and treatment type (psychoanalysis and psychoanalytic psychotherapy) and investigated scores on 39 Rorschach-CS variables. The total sample consisted of 176 participants from four mental health care organizations in The Netherlands. We first examined pretreatment differences between patients entering psychoanalysis and patients entering psychoanalytic psychotherapy. The two treatment groups did not seem to differ substantially before treatment, with the exception of the level of ideational problems. Next, we studied the outcome of psychoanalysis and psychoanalytic psychotherapy by comparing the Rorschach-CS scores of the six groups of patients. In general, we found significant differences between pretreatment and posttreatment on a relatively small number of Rorschach-CS variables. More pre/post differences were found between the psychoanalytic psychotherapy groups than between the psychoanalysis groups. More research is needed to examine whether analyzing clusters of variables might reveal other results.


2018 ◽  
Vol 1 (2) ◽  
pp. 58
Author(s):  
Setia Budi ◽  
Ria Dila Syahfitri

The rate of stroke incidence is about 200 per 100,000 people throughout the world. This study aims to determine the Relation Suffer Stroke With Independence Level In Neurology Polyclinic TK II DR Ak Gani Palembang Year Hospital 2017. The research method used is descriptive quantitative with cross sectional design that is done by interviewing techniques with questionnaires on 42 respondents with Accidental sampling technique. This research was conducted in August 2017. Data analysis used is univariate data analysis and bivariate data analysis with one way anova test result. The results of univariate analysis showed that the duration of the respondents suffering from stroke was between 2.10 years to 3.38 years. Also found that most respondents were at the level of independence f; independent, except bathing, dressing, moving, and one other function with a total of 12 respondents. The results showed that there was a significant relationship between the long suffering stroke with the level of independence with the value of p value 0.025. For that the need for rehabilitation to patients and families of patients in order to help improve the independence of stroke patients in doing their daily activities. Keywords : Long Suffer Stroke, Level of Independence


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