scholarly journals Histochemical properties of sialic acids and antimicrobial substances in canine anal glands

10.4081/1850 ◽  
2011 ◽  
Vol 55 (3) ◽  
Author(s):  
T. Nara ◽  
T. Yasui ◽  
O. Fujimori ◽  
W. Meyer ◽  
A. Tsukise
Keyword(s):  
Sialic Acids ◽  
Anal Glands ◽  
Antimicrobial Substances

scholarly journals Histochemical properties of sialic acids and antimicrobial substances in canine anal glands

2011 ◽  
Vol 55 (3) ◽  
pp. 29 ◽  
Author(s):  
T. Nara ◽  
T. Yasui ◽  
O. Fujimori ◽  
W. Meyer ◽  
A. Tsukise
Keyword(s):  
Sialic Acids ◽  
Anal Glands ◽  
Antimicrobial Substances

Histochemical demonstration of sialic acids and antimicrobial substances in the porcine anal glands

2012 ◽  
Vol 114 (4) ◽  
pp. 327-333 ◽  
Author(s):  
Takayuki Nara ◽  
Tadashi Yasui ◽  
Wilfried Meyer ◽  
Azuma Tsukise
Keyword(s):  
Histochemical Demonstration ◽  
Sialic Acids ◽  
Anal Glands ◽  
Antimicrobial Substances

Histochemical distribution of sialic acids and antimicrobial substances in porcine carpal glands

2012 ◽  
Vol 304 (8) ◽  
pp. 599-607 ◽  
Author(s):  
Kousuke Fukui ◽  
Tadashi Yasui ◽  
Hiroshi Gomi ◽  
Hiroshi Sugiya ◽  
Osamu Fujimori ◽  
...  
Keyword(s):  
Sialic Acids ◽  
Antimicrobial Substances

scholarly journals A male sexually dimorphic trait provides antimicrobials to eggs in blenny fish

2006 ◽  
Vol 2 (3) ◽  
pp. 330-333 ◽  
Author(s):  
Eva Giacomello ◽  
Daniela Marchini ◽  
Maria B Rasotto
Keyword(s):  
Internal Surface ◽  
Sexually Dimorphic ◽  
Offspring Survival ◽  
Early Life Stages ◽  
Anal Glands ◽  
Antimicrobial Substances ◽  
Microbial Infections ◽  
Egg Care ◽  
Salaria Pavo ◽  
Peacock Blenny

Predation and microbial infections are the major causes of natural mortality for early life stages of oviparous species. The parental traits reducing the effects of predation are rather well described, whereas antimicrobial mechanisms enhancing offspring survival are largely unexplored. In this paper, we report that a male sexually dimorphic trait, the anal glands, of the redlip blenny ( Ophioblennius atlanticus atlanticus ) and the peacock blenny ( Salaria pavo ), two fish species with paternal egg care, produce a mucus enriched with antimicrobial substances. Histological and histochemical analyses showed that the anal glands of these species are characterized by the massive presence of mucus-secreting cells. Anal gland extracts, from both the hydrophilic and the hydrophobic protein fraction, exhibited a lysozyme-like activity. Field observations demonstrated that redlip blenny males, while performing egg care, rub the anal region over the nest internal surface, probably facilitating the transfer of mucus to eggs. These results strongly indicate that this sexually dimorphic trait is involved in egg defence against microbial infections.

Sialic acids and antimicrobial substances in the apocrine glands of porcine perianal skin

2012 ◽  
Vol 304 (8) ◽  
pp. 609-617
Author(s):  
Takayuki Nara ◽  
Tadashi Yasui ◽  
Osamu Fujimori ◽  
Wilfried Meyer ◽  
Azuma Tsukise
Keyword(s):  
Sialic Acids ◽  
Perianal Skin ◽  
Apocrine Glands ◽  
Antimicrobial Substances

scholarly journals Histochemical localization of sialic acids and antimicrobial substances in eccrine glands of porcine snout skin

2012 ◽  
Vol 56 (1) ◽  
pp. 6 ◽  
Author(s):  
K. Fukui ◽  
T. Yasui ◽  
H. Gomi ◽  
H. Sugiya ◽  
O. Fujimori ◽  
...  
Keyword(s):  
Sialic Acids ◽  
Histochemical Localization ◽  
Antimicrobial Substances ◽  
Eccrine Glands

COMPARATIVE ACTION OF VARIOUS OESTROGENIC COMPOUNDS ON MOUSE VAGINAL SIALIC ACIDS (II)

1969 ◽  
Vol 62 (4) ◽  
pp. 663-670 ◽  
Author(s):  
Lars Carlborg
Keyword(s):  
Sialic Acid ◽  
Response Curve ◽  
Sialic Acids ◽  
Clear Cut ◽  
Suitable Parameter ◽  
Sufficient Degree ◽  
Comparative Action ◽  
Relative Potencies ◽  
Good Agreement

ABSTRACT Oestrogens administered in lower doses than necessary to induce full cornification of the mouse vagina induce mucification. It was shown previously that the degree of mucification could be estimated by quantitative determination of sialic acids. A suitable parameter for oestrogen assay was the measurement of vaginal sialic acid concentration which exhibited a clear cut dose response curve. Eleven assays of various oestrogens were performed with this method. Their estimated relative potencies were in good agreement with other routine oestrogen assays. A statistically sufficient degree of precision was found. The sensitivity was of the same order, or slightly higher, than the Allen-Doisy test.

Interspecific variation of zona pellucida glycoconjugates in several species of marsupial

Reproduction ◽  
2000 ◽  
pp. 111-120 ◽  
Author(s):  
JA Chapman ◽  
OW Wiebkin ◽  
WG Breed
Keyword(s):  
Glycine Max ◽  
Sialic Acid ◽  
Zona Pellucida ◽  
Fluorescein Isothiocyanate ◽  
Interspecific Variation ◽  
Lectin Histochemistry ◽  
Sialic Acids ◽  
Arachis Hypogea ◽  
Marsupial Species ◽  
Similar Intensity

The zona pellucida glycoconjugate content of several marsupial species was investigated using differential lectin histochemistry. Ovaries from fat-tailed dunnarts, a southern brown bandicoot, grey short-tailed opossums, brushtail possums, ringtail possums, koalas and eastern grey kangaroos were fixed, embedded in paraffin wax, sectioned and stained with ten fluorescein isothiocyanate-conjugated lectins. Sections were also incubated with either neuraminidase or saponified, respectively, before incubation with the lectins to identify saccharide residues masked by sialic acids or O-acetyl groups on sialic acids. The zonae pellucidae surrounding the oocytes of the marsupials demonstrated interspecific variation in glycoconjugate content, with mannose-containing glycoconjugates exhibiting the greatest variation. Some of the zona pellucida glycoconjugates of all species, except those of the opossums, were masked by sialic acid with an increase in fluorescence with lectins from Arachis hypogea (PNA), and Glycine max (SBA), after desialylation. The disaccharide beta-galactose(1-4)N-acetyl-D-glucosamine appeared to be conformationally masked by O-acetyl groups of sialic acids in the zonae pellucidae of all species, with an increase in fluorescence with the lectin from Erythrina cristagalli (ECA), after saponification. Similar intensity and localization of beta-(1-4)-N-acetyl-D-glucosamine, as shown by staining of the lectin from Triticum vulgaris (WGA), to the inner and outer regions of the zona pellucida, were found to those reported in eutherian species. WGA fluorescence became uniform throughout the zonae pellucidae after saponification, indicating differential O-acetylation of sialic acids on the internal compartment of the zonae pellucidae.

Antimicrobial Activity of Iron-Halide Complexes Against Gram-Positive and Gram-Negative Bacteria

2020 ◽  
Author(s):  
Nusrat Abedin ◽  
Abdullah Hamed A Alshehri ◽  
Ali M A Almughrbi ◽  
Olivia Moore ◽  
Sheikh Alyza ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Resistance ◽  
Extracellular Dna ◽  
Antibiofilm Activity ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative ◽  
Antimicrobial Substances ◽  
Mammalian Cell Lines

Antimicrobial resistance (AMR) has become one of the more serious threats to the global health. The emergence of bacteria resistant to antimicrobial substances decreases the potencies of current antibiotics. Consequently, there is an urgent and growing need for the developing of new classes of antibiotics. Three prepared novel iron complexes have a broad-spectrum antimicrobial activity with minimum bactericidal concentration (MBC) values ranging from 3.5 to 10 mM and 3.5 to 40 mM against Gram-positive and Gram-negative bacteria with antimicrobial resistance phenotype, respectively. Time-kill studies and quantification of the extracellular DNA confirmed the bacteriolytic mode of action of the iron-halide compounds. Additionally, the novel complexes showed significant antibiofilm activity against the tested pathogenic bacterial strains at concentrations lower than the MBC. The cytotoxic effect of the complexes on different mammalian cell lines show sub-cytotoxic values at concentrations lower than the minimum bactericidal concentrations.

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