scholarly journals Cellulose in algal cell wall : an “in situ” localization

10.4081/1613 ◽  
2009 ◽  
Vol 45 (1) ◽  
pp. 51 ◽  
Author(s):  
B Baldan ◽  
P Andolfo ◽  
L Navazio ◽  
C Tolomio ◽  
P Mariani
Keyword(s):  
Cell Wall ◽  
Algal Cell ◽  
Algal Cell Wall

scholarly journals Algal cell wall-degrading enzymes from viscera of marine animals.

1989 ◽  
Vol 55 (1) ◽  
pp. 105-110 ◽  
Author(s):  
Kuniko Yamaguchi ◽  
Toshiyoshi Araki ◽  
Takahiko Aoki ◽  
Chao-Huang Tseng ◽  
Manabu Kitamikado
Keyword(s):  
Cell Wall ◽  
Algal Cell ◽  
Cell Wall Degrading Enzymes ◽  
Marine Animals ◽  
Degrading Enzymes ◽  
Algal Cell Wall

scholarly journals Insights into cell wall disintegration of Chlorella vulgaris

PLoS ONE ◽  
2022 ◽  
Vol 17 (1) ◽  
pp. e0262500
Author(s):  
Sophie Weber ◽  
Philipp M. Grande ◽  
Lars M. Blank ◽  
Holger Klose
Keyword(s):  
Cell Wall ◽  
Chlorella Vulgaris ◽  
Cell Walls ◽  
Algal Cell ◽  
Monosaccharide Composition ◽  
Total Biomass ◽  
Algal Cell Wall ◽  
Wall Structures ◽  
Valuable Compounds ◽  
Hydrolysis Of

With their ability of CO2 fixation using sunlight as an energy source, algae and especially microalgae are moving into the focus for the production of proteins and other valuable compounds. However, the valorization of algal biomass depends on the effective disruption of the recalcitrant microalgal cell wall. Especially cell walls of Chlorella species proved to be very robust. The wall structures that are responsible for this robustness have been studied less so far. Here, we evaluate different common methods to break up the algal cell wall effectively and measure the success by protein and carbohydrate release. Subsequently, we investigate algal cell wall features playing a role in the wall’s recalcitrance towards disruption. Using different mechanical and chemical technologies, alkali catalyzed hydrolysis of the Chlorella vulgaris cells proved to be especially effective in solubilizing up to 56 wt% protein and 14 wt% carbohydrates of the total biomass. The stepwise degradation of C. vulgaris cell walls using a series of chemicals with increasingly strong conditions revealed that each fraction released different ratios of proteins and carbohydrates. A detailed analysis of the monosaccharide composition of the cell wall extracted in each step identified possible factors for the robustness of the cell wall. In particular, the presence of chitin or chitin-like polymers was indicated by glucosamine found in strong alkali extracts. The presence of highly ordered starch or cellulose was indicated by glucose detected in strong acidic extracts. Our results might help to tailor more specific efforts to disrupt Chlorella cell walls and help to valorize microalgae biomass.

scholarly journals Microbial Population Changes in Decaying Ascophyllum nodosum Result in Macroalgal-Polysaccharide-Degrading Bacteria with Potential Applicability in Enzyme-Assisted Extraction Technologies

Marine Drugs ◽  
2019 ◽  
Vol 17 (4) ◽  
pp. 200 ◽  
Author(s):  
Maureen Ihua ◽  
Freddy Guihéneuf ◽  
Halimah Mohammed ◽  
Lekha Margassery ◽  
Stephen Jackson ◽  
...  
Keyword(s):  
Cell Wall ◽  
Algal Cell ◽  
Hydrolytic Enzyme ◽  
Ascophyllum Nodosum ◽  
Extraction Methods ◽  
Bacterial Isolates ◽  
Algal Cell Wall ◽  
Assisted Extraction ◽  
Degrading Bacteria ◽  
Enzyme Assisted Extraction

Seaweeds are of significant interest in the food, pharmaceutical, and agricultural industries as they contain several commercially relevant bioactive compounds. Current extraction methods for macroalgal-derived metabolites are, however, problematic due to the complexity of the algal cell wall which hinders extraction efficiencies. The use of advanced extraction methods, such as enzyme-assisted extraction (EAE), which involve the application of commercial algal cell wall degrading enzymes to hydrolyze the cell wall carbohydrate network, are becoming more popular. Ascophyllum nodosum samples were collected from the Irish coast and incubated in artificial seawater for six weeks at three different temperatures (18 °C, 25 °C, and 30 °C) to induce decay. Microbial communities associated with the intact and decaying macroalga were examined using Illumina sequencing and culture-dependent approaches, including the novel ichip device. The bacterial populations associated with the seaweed were observed to change markedly upon decay. Over 800 bacterial isolates cultured from the macroalga were screened for the production of algal cell wall polysaccharidases and a range of species which displayed multiple hydrolytic enzyme activities were identified. Extracts from these enzyme-active bacterial isolates were then used in EAE of phenolics from Fucus vesiculosus and were shown to be more efficient than commercial enzyme preparations in their extraction efficiencies.

Surface Complexation Modeling of Proton and Cd Adsorption onto an Algal Cell Wall

2005 ◽  
Vol 39 (11) ◽  
pp. 4060-4065 ◽  
Author(s):  
Emily S. Kaulbach ◽  
Jennifer E. S. Szymanowski ◽  
Jeremy B. Fein
Keyword(s):  
Cell Wall ◽  
Algal Cell ◽  
Surface Complexation ◽  
Surface Complexation Modeling ◽  
Algal Cell Wall ◽  
Cd Adsorption
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