scholarly journals Determination of cholesterol oxides in anchovies (Engraulis encrasicolus) treated with a commercial mixture of citric acid, trisodium acid and hydrogen peroxide

10.4081/1429 ◽  
2013 ◽  
Vol 1 (6) ◽  
Author(s):  
Marrone Raffaele ◽  
Giorgio Smaldone ◽  
Giuseppe Palma ◽  
Raffaele Romano ◽  
Domenico Bortone ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Citric Acid ◽  
Cholesterol Oxides ◽  
Engraulis Encrasicolus ◽  
Commercial Mixture

scholarly journals Determination of cholesterol oxides in anchovies (Engraulis encrasicolus) treated with a commercial mixture of citric acid, trisodium acid and hydrogen peroxide

2013 ◽  
Vol 1 (6) ◽  
pp. 42
Author(s):  
Raffaele Marrone ◽  
Giorgio Smaldone ◽  
Giuseppe Palma ◽  
Raffaele Romano ◽  
Domenico Bortone ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Citric Acid ◽  
Cholesterol Oxides ◽  
Engraulis Encrasicolus ◽  
Commercial Mixture

Enzymatic colorimetry of lecithin and sphingomyelin in aqueous solution.

1983 ◽  
Vol 29 (8) ◽  
pp. 1513-1517 ◽  
Author(s):  
M W McGowan ◽  
J D Artiss ◽  
B Zak
Keyword(s):  
Aqueous Solution ◽  
Hydrogen Peroxide ◽  
Amniotic Fluid ◽  
Enzymatic Reaction ◽  
Detergent Solution ◽  
Enzymatic Determination ◽  
Red Color ◽  
Hydrolysis Of ◽  
Zwitterionic Detergent

Abstract A procedure for the enzymatic determination of lecithin and sphingomyelin in aqueous solution is described. The phospholipids are first dissolved in chloroform:methanol (2:1 by vol), the solvent is evaporated, and the residue is redissolved in an aqueous zwitterionic detergent solution. The enzymatic reaction sequences of both assays involve hydrolysis of the phospholipids to produce choline, which is then oxidized to betaine, thus generating hydrogen peroxide. The hydrogen peroxide is subsequently utilized in the enzymatic coupling of 4-aminoantipyrine and sodium 2-hydroxy-3,5-dichlorobenzenesulfonate, an intensely red color being formed. The presence of a non-reacting phospholipid enhances the hydrolysis of the reacting phospholipid. Thus we added lecithin to the sphingomyelin standards and sphingomyelin to the lecithin standards. This precise procedure may be applicable to determination of lecithin and sphingomyelin in amniotic fluid.

scholarly journals CHROMATOGRAPHIC DETERMINATION OF THE ACIDS OF THE CITRIC ACID CYCLE IN TISSUES

1951 ◽  
Vol 193 (1) ◽  
pp. 277-283 ◽  
Author(s):  
CharlesE. Frohman ◽  
JamesM. Orten ◽  
ArthurH. Smith
Keyword(s):  
Citric Acid ◽  
Citric Acid Cycle ◽  
Chromatographic Determination ◽  
Acid Cycle
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