Double Labeling of Vagal Preganglionic and Sympathetic Postganglionic Fibers in Celiac Ganglion, Superior Mesenteric Arteries and Myenteric Plexus

2017 ◽  
Vol 60 (1) ◽  
pp. 41-53 ◽  
Author(s):  
Shi-Jane Ting
Keyword(s):  
Myenteric Plexus ◽  
Mesenteric Arteries ◽  
Double Labeling ◽  
Celiac Ganglion

Intestinal anaphylaxis induces Fos immunoreactivity in myenteric plexus of rat small intestine

1997 ◽  
Vol 272 (1) ◽  
pp. G181-G189 ◽  
Author(s):  
M. Miampamba ◽  
D. T. Tan ◽  
M. R. Oliver ◽  
K. A. Sharkey ◽  
R. B. Scott
Keyword(s):  
Myenteric Plexus ◽  
Cell Activation ◽  
Mast Cell Activation ◽  
In Vivo Studies ◽  
Double Labeling ◽  
Myenteric Neurons ◽  
Control Tissue ◽  
Afferent Nerves

Fos immunohistochemistry was used to identify myenteric neurons activated as a consequence of intestinal anaphylaxis in Hooded-Lister rats sensitized to egg albumin (EA 10 micrograms ip). After incubation in test solutions, or after in vivo challenge, jejunal tissues were processed for immunohistochemistry with an anti-Fos antibody (1:500, TF161). The neuronal identity of the Fos-labeled nuclei was confirmed by double labeling with neuron-specific enclose (1:1,000). In in vitro studies, exposure of control tissue to 50 mM K(+)-Krebs-EA (2 x 10(-5) M) solutions significantly increased Fos immunoreactivity in the myenteric plexus, whereas a basal level of Fos was seen in control tissue incubated in Krebs solution, sham-sensitized tissue exposed to bovine serum albumin (BSA, 2 x 10(-5) M), or EA and sensitized tissue exposed to BSA. Pretreatment of sensitized tissue with doxantrazole (10(-4) M) markedly reduced Fos immunoreactivity observed after EA exposure. In in vivo studies, there was negligible Fos immunoreactivity in the myenteric plexus of control, sham-sensitized, or sensitized rats challenged with saline. A low level of Fos was seen in neurons of sham-sensitized rats challenged with BSA or EA and in sensitized rats challenged with BSA. Significantly greater levels of Fos were observed in the myenteric plexus of sensitized animals challenged with EA, even after pretreatment with capsaicin (125 mg/kg). These results suggest a role for myenteric neurons in intestinal anaphylaxis. In sensitized rats, activation of myenteric neurons is dependent on antigen-induced mast cell activation and occurs independently of capsaicin-sensitive afferent nerves.

Projections of bombesin-like immunoreactive fibers from the rat stomach to the celiac ganglion revealed by a double-labeling technique

1987 ◽  
Vol 416 (1) ◽  
pp. 192-194 ◽  
Author(s):  
Masayasu Hamaji ◽  
Yoshinori Kawai ◽  
Yasunaru Kawashima ◽  
Masaya Tohyama
Keyword(s):  
Rat Stomach ◽  
Double Labeling ◽  
Celiac Ganglion

scholarly journals Distribution and biological activity of obestatin in the rat

2006 ◽  
Vol 191 (2) ◽  
pp. 481-489 ◽  
Author(s):  
Siok L Dun ◽  
G Cristina Brailoiu ◽  
Eugen Brailoiu ◽  
Jun Yang ◽  
Jaw Kang Chang ◽  
...  
Keyword(s):  
Biological Activity ◽  
Gastric Mucosa ◽  
Myenteric Plexus ◽  
Cortical Neurons ◽  
Leydig Cells ◽  
Ganglion Cells ◽  
Myenteric Ganglion ◽  
Double Labeling ◽  
Central Neurons ◽  
In Cells

Obestatin, a 23 amino acid peptide recently isolated from the rat stomach, is encoded by the same gene that encodes ghrelin. With the use of an antiserum directed against the mouse/rat obestatin, obestatin immunoreactivity (irOBS) was detected in cells of the gastric mucosa, myenteric plexus, and in Leydig cells of the testis in Sprague–Dawley rats. Double labeling the myenteric plexus with obestatin antiserum and choline acetyltransferase (ChAT) antiserum revealed that nearly all irOBS neurons were ChAT positive and vice versa. For comparative purposes, myenteric ganglion cells, cells in the gastric mucosa, and Leydig cells of the testis were shown to be immunoreactive to preproghrelin. The biological activity of obestatin on rat central neurons was assessed by the calcium microfluorimetric Fura-2 method. Obestatin (100 nM) administered to dissociated and cultured rat cerebral cortical neurons elevated cytosolic calcium concentrations [Ca2+]i in a population of cortical neurons. The result provides the first immunohistochemical evidence that obestatin is expressed in cells of the gastric mucosa and myenteric ganglion cells, and also in Leydig cells of the testis; the peptide is biologically active on central neurons.

Gold labeling of teichoic acid on adjacent surfaces of staphylococcus aureus

1990 ◽  
Vol 48 (3) ◽  
pp. 578-579
Author(s):  
Margaret Hukee
Keyword(s):  
Staphylococcus Aureus ◽  
Teichoic Acid ◽  
Protein Labeling ◽  
Minimal Amount ◽  
Section Thickness ◽  
Double Labeling ◽  
Parallel Surfaces ◽  
Supporting Membrane

Gold labeling of two antigens (double labeling) is often done on two section surfaces separated by section thickness. Whether labeling is done on both sides of the same section or on two parallel surfaces separated by section thickness (PSSST), comparable results are dependent on an equal number of epitopes being exposed at each surface. We propose a method to study protein labeling within the same field of proteins, by examining two directly adjacent surfaces that were split during sectioning. The number of labeling sites on adjacent surfaces (AS) were compared to sites on PSSST surfaces in individual bacteria.Since each bacteria needed to be recognizable in all three section surfaces, one-hole grids were used for labeling. One-hole grids require a supporting membrane and excessive handling during labeling often ruptures the membrane. To minimize handling, a labeling chamber was designed that is inexpensive, disposable, minimizes contamination, and uses a minimal amount of solution.

Neurochemical phenotype and function of endomorphin-2-containing neurons in the myenteric plexus of the rat colon

2010 ◽  
Vol 34 (8) ◽  
pp. S19-S19
Author(s):  
Jun‑Ping Li ◽  
Chang‑Jun Gao ◽  
Bo‑Chang Lü ◽  
Ting Zhang ◽  
Jiang‑Bo Ma ◽  
...  
Keyword(s):  
Myenteric Plexus ◽  
Rat Colon ◽  
And Function

Sox2 expression in the myenteric plexus in the caecum of postnatal and adult mouse

2016 ◽  
Vol 54 (08) ◽  
Author(s):  
D Grundmann ◽  
E Loris ◽  
L Aigner ◽  
S Couillard-Despres ◽  
KH Schäfer
Keyword(s):  
Myenteric Plexus ◽  
Adult Mouse ◽  
Sox2 Expression
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