scholarly journals Endothelial Nitric Oxide Synthase Gene Variation Associated With Chronic Kidney Disease After Liver Transplant

2010 ◽  
Vol 85 (9) ◽  
pp. 814-820 ◽  
Author(s):  
Kiran Bambha ◽  
W. Ray Kim ◽  
Charles B. Rosen ◽  
Rachel A. Pedersen ◽  
Cynthia Rys ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Chronic Kidney Disease ◽  
Nitric Oxide Synthase ◽  
Kidney Disease ◽  
Liver Transplant ◽  
Endothelial Nitric Oxide Synthase ◽  
Gene Variation ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

scholarly journals Endothelial cell transfusion ameliorates endothelial dysfunction in 5/6 nephrectomized rats

2013 ◽  
Vol 305 (8) ◽  
pp. H1256-H1264 ◽  
Author(s):  
Maricica Pacurari ◽  
Dongqi Xing ◽  
Rob H. P. Hilgers ◽  
Yuan Yuan Guo ◽  
Zhengqin Yang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Chronic Kidney Disease ◽  
Endothelial Dysfunction ◽  
Nitric Oxide Synthase ◽  
Kidney Disease ◽  
Endothelial Nitric Oxide Synthase ◽  
Vascular Function ◽  
Vascular Reactivity ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

Endothelial dysfunction is prevalent in chronic kidney disease. This study tested the hypothesis that transfusion of rat aortic endothelial cells (ECs) ameliorates endothelial dysfunction in a rat model of chronic kidney disease. Male Sprague-Dawley rats underwent sham surgery or 5/6 nephrectomy (Nx). Five weeks after Nx, EC (1.5 × 106 cells/rat) or vehicle were transfused intravenously. One week later, vascular reactivity of mesenteric artery was assessed on a wire myograph. Sensitivity of endothelium-dependent relaxation to acetylcholine and maximum vasodilation were impaired by Nx and improved by EC transfusion. Using selective pharmacological nitric oxide synthase isoform inhibitors, we demonstrated that the negative effect of Nx on endothelial function and rescue by EC transfusion are, at least in part, endothelial nitric oxide synthase mediated. Plasma asymmetric dimethylarginine was increased by Nx and decreased by EC transfusion, whereas mRNA expression of dimethylarginine dimethylaminohydrolases 1 (DDAH1) was decreased by Nx and restored by EC transfusion. Immunohistochemical staining confirmed that local expression of DDAH1 is decreased by Nx and increased by EC transfusion. In conclusion, EC transfusion attenuates Nx-induced endothelium-dependent vascular dysfunction by regulating DDAH1 expression and enhancing endothelial nitric oxide synthase activity. These results suggest that EC-based therapy could provide a novel therapeutic strategy to improve vascular function in chronic kidney disease.

Endothelial nitric oxide synthase gene intron4 VNTR polymorphism in patients with chronic kidney disease

2011 ◽  
Vol 22 (6) ◽  
pp. 487-492 ◽  
Author(s):  
Manal F. Elshamaa ◽  
Samar Sabry ◽  
Ahmed Badr ◽  
Mostafa El-Ahmady ◽  
Eman A. Elghoroury ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Chronic Kidney Disease ◽  
Nitric Oxide Synthase ◽  
Kidney Disease ◽  
Endothelial Nitric Oxide Synthase ◽  
Vntr Polymorphism ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

scholarly journals Reduced endothelial nitric oxide synthase activation contributes to cardiovascular injury during chronic kidney disease progression

2019 ◽  
Vol 317 (2) ◽  
pp. F275-F285 ◽  
Author(s):  
Isabel Amador-Martínez ◽  
Rosalba Pérez-Villalva ◽  
Norma Uribe ◽  
César Cortés-González ◽  
Norma A. Bobadilla ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Brain Natriuretic Peptide ◽  
Endothelial Nitric Oxide Synthase ◽  
Ischemia Reperfusion ◽  
Cardiac Injury ◽  
Ckd Progression ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

Major cardiovascular events are a common complication in patients with chronic kidney disease (CKD). Endothelial dysfunction can contribute to the cardiovascular injury observed in CKD. Here, we used a rat model of acute kidney injury to CKD transition to investigate heart alterations in the pathway activating endothelial nitric oxide synthase (eNOS) and its impact on the cardiac injury observed during CKD progression. Fifty male Wistar rats were subjected to sham surgery ( n = 25) or bilateral renal ischemia-reperfusion (IR-CKD) for 45 min ( n = 25). Rats were studied on a monthly basis up to 5 mo ( n = 5). In another set of sham and IR-CKD rats, l-arginine was administered starting on the third month after renal ischemia. CKD development and cardiac alterations were monitored in all groups. CKD was characterized by a progressive increase in proteinuria and renal dysfunction that was evident after the fifth month of followup. Heart hypertrophy was observed starting on the fourth month after ischemia-reperfusion. There was a significant increase in brain natriuretic peptide levels. In the heart, IR-CKD rats had increased eNOS phosphorylation at threonine 495 and reduced eNOS-heat shock protein-90α interactions. l-Arginine administration prevented the heart alterations observed during CKD and increased eNOS coupling/dimerization and activation. In summary, CKD progression is accompanied by cardiac hypertrophy, fibrosis, oxidative stress, and increased brain natriuretic peptide levels. These alterations were associated with limited eNOS activation in the heart, which may result in reduced nitric oxide bioavailability and contribute to cardiac injury during CKD.

Does Endothelial Nitric Oxide Synthase Gene Variation Play a Role in the Occurrence of Hypertension in Pregnancy?

2003 ◽  
Vol 22 (2) ◽  
pp. 149-155 ◽  
Author(s):  
Elvira Grandone ◽  
Donatella Colaizzo ◽  
Pasquale Martinelli ◽  
Giuseppe Pavone ◽  
Massimo Errico ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Endothelial Nitric Oxide Synthase ◽  
Gene Variation ◽  
Hypertension In Pregnancy ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide ◽  
In Pregnancy

Gene Variation in Resistant Hypertension: Multilocus Analysis of the Angiotensin 1-Converting Enzyme, Angiotensinogen, and Endothelial Nitric Oxide Synthase Genes

2011 ◽  
Vol 30 (8) ◽  
pp. 555-564 ◽  
Author(s):  
Juan Carlos Yugar-Toledo ◽  
José Fernando Vilela Martin ◽  
José Eduardo Krieger ◽  
Alexandre C Pereira ◽  
Caroline Demacq ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Resistant Hypertension ◽  
Endothelial Nitric Oxide Synthase ◽  
Converting Enzyme ◽  
Gene Variation ◽  
Multilocus Analysis ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide
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