scholarly journals Establishment of Methylation-Specific PCR for the Mouse p53 Gene

2011 ◽  
Vol 2011 ◽  
pp. 1-4 ◽  
Author(s):  
Ryuji Okazaki ◽  
Akira Ootsuyama ◽  
Yasuhiro Yoshida ◽  
Toshiyuki Norimura
Keyword(s):  
Cpg Islands ◽  
P53 Gene ◽  
Pcr Primers ◽  
Young Mouse ◽  
Methylation Specific Pcr ◽  
Specific Pcr ◽  
Pcr Product ◽  
Induced Tumors ◽  
Radiation Induced ◽  
Radiation Induced Tumors

Methylation-specific PCR (MSP) of the mouse p53 gene has not yet been reported. We searched the CpG islands, sequenced the bisulfited DNA, and designed PCR primers for methylation and unmethylation sites. DNA from a young mouse produced a strong PCR product with the unmethylated primer and a weaker band with the methylated primer. DNA from an old mouse produced bands of similar intensities with both primers. In radiation-induced tumors, DNA from an old mouse yielded similar bands with both types of primers. We suggest that MSP is a valuable technique for the epigenetic study of the mouse p53 gene.

Prognosis of glioblastoma with faint MGMT methylation-specific PCR product

2015 ◽  
Vol 122 (1) ◽  
pp. 179-188 ◽  
Author(s):  
Chih-Yi Hsu ◽  
Hsiang-Ling Ho ◽  
Shih-Chieh Lin ◽  
Yi-Chun Chang-Chien ◽  
Ming-Hsiung Chen ◽  
...  
Keyword(s):  
Mgmt Methylation ◽  
Methylation Specific Pcr ◽  
Specific Pcr ◽  
Pcr Product

Radiation-induced tumors in irradiated stage I testicular seminoma: Results of a 25-year follow-up (1968–1993)

1998 ◽  
Vol 67 (1) ◽  
pp. 38-40 ◽  
Author(s):  
Moshe E. Stein ◽  
Michelle Leviov ◽  
Keren Drumea ◽  
Boaz Moshkovitz ◽  
Ofer Nativ ◽  
...  
Keyword(s):  
Stage I ◽  
Testicular Seminoma ◽  
Induced Tumors ◽  
Radiation Induced ◽  
Radiation Induced Tumors

RETROVIRUS PARTICLES IN SPONTANEOUSLY OCCURRING AND RADIATION-INDUCED TUMORS IN ddY MICE

1981 ◽  
Vol 31 (2) ◽  
pp. 233-247
Author(s):  
Fumitoshi Chino ◽  
Fumiaki Sato ◽  
Shunsaku Sasaki
Keyword(s):  
Induced Tumors ◽  
Radiation Induced ◽  
Radiation Induced Tumors

Intracranial Meningioma Subsequent to Radiation for a Pituitary Tumor: Case Report

Neurosurgery ◽  
1989 ◽  
Vol 25 (4) ◽  
pp. 643-645 ◽  
Author(s):  
K. Sridhar ◽  
B. Ramamurthi
Keyword(s):  
Radiation Therapy ◽  
Case Report ◽  
Pituitary Tumor ◽  
Malignant Potential ◽  
Growth Characteristics ◽  
Intracranial Meningioma ◽  
Induced Tumors ◽  
Radiation Induced ◽  
Biological Group ◽  
Radiation Induced Tumors

Abstract The authors report a case of an intracranial meningioma that developed 33 years after radiation therapy for a pituitary tumor. Radiation-induced tumors form a distinct biological group with aggressive growth characteristics and a malignant potential.

scholarly journals Studying The Expression of Human P53 Gene in Patients Carrying Breast Cancer.

2017 ◽  
Vol 28 (1) ◽  
pp. 14
Author(s):  
Dawood S. Edan
Keyword(s):  
Breast Cancer ◽  
Healthy Woman ◽  
P53 Gene ◽  
Suppressor Gene ◽  
Women Patients ◽  
Cell Protection ◽  
Specific Pcr ◽  
Pcr Product ◽  
Dna Repair Proteins ◽  
Healthy Sample

The gene P53 can activate DNA repair proteins when DNA has sustained damage. So, it is an important factor in aging. Also, P53 gene can also be modified by mutagens (chemicals, radiation, or viruses), increasing the likelihood for uncontrolled cell division. For this importance, we collected thirty samples from Yarmouk hospital in Baghdad, Iraq. Twenty-nine from women patients carrying breast cancer and the last one was from healthy woman, and the samples that we have taken were embedded in paraffin wax. The extracted RNA from each samples was used to check the expression of a suppressor gene (P53) via Real time PCR. We designed primers of P53 gene on encoded sequence (Exon), to make sure generation a specific PCR product of P53 gene by converting mRNA to cDNA then PCR Product. The expression of each samples was fluctuated between 0.1 fold to 0.2 fold compared with the control (the healthy sample). The highest expression showed was samples 7 (0.2 fold), and the lowest expression showed was samples 27 (0.1 fold) that compared with control expression (sample 19). While the control expression was the highest (0.25 fold) among all samples. The results indicate that tumor may affects on a suppressor gene (P53) and can decrease the gene expression, and eventually can decrease the P53 suppressor protein that can be used it in the cell protection against cancer.

Evidence of oncogenic cofactors in radiation-induced tumors in mice

1975 ◽  
Vol 7 (1) ◽  
pp. 57-61
Author(s):  
M. B. Patricio ◽  
W. H. Clode ◽  
J. A. Ricardo
Keyword(s):  
Induced Tumors ◽  
Radiation Induced ◽  
Radiation Induced Tumors
Sign in / Sign up

Export Citation Format

Share Document