scholarly journals Derivation of Two New Human Embryonic Stem Cell Lines from Nonviable Human Embryos

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Svetlana Gavrilov ◽  
Darja Marolt ◽  
Nataki C. Douglas ◽  
Robert W. Prosser ◽  
Imran Khalid ◽  
...  
Keyword(s):  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Poor Quality ◽  
Human Embryos ◽  
Clinical Use ◽  
Stem Cell Lines ◽  
Hesc Lines ◽  
Hesc Derivation

We report the derivation and characterization of two new human embryonic stem cells (hESC) lines (CU1 and CU2) from embryos with an irreversible loss of integrated organismic function. In addition, we analyzed retrospective data of morphological progression from embryonic day (ED) 5 to ED6 for 2480 embryos not suitable for clinical use to assess grading criteria indicative of loss of viability on ED5. Our analysis indicated that a large proportion ofin vitrofertilization (IVF) embryos not suitable for clinical use could be used for hESC derivation. Based on these combined findings, we propose that criteria commonly used in IVF clinics to determine optimal embryos for uterine transfer can be employed to predict the potential for hESC derivation from poor quality embryos without the destruction of vital human embryos.

Derivation and characterization of human embryonic stem cell lines from poor quality embryos

2009 ◽  
Vol 36 (4) ◽  
pp. 229-239 ◽  
Author(s):  
Weiqiang Liu ◽  
Yifei Yin ◽  
Xiaolin Long ◽  
Yumei Luo ◽  
Yonghua Jiang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Lines ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Poor Quality ◽  
Stem Cell Lines

scholarly journals Blockade of STAT3 Causes Severe In Vitro and In Vivo Maturation Defects in Intestinal Organoids Derived from Human Embryonic Stem Cells

2019 ◽  
Vol 8 (7) ◽  
pp. 976 ◽  
Author(s):  
Kwang Bo Jung ◽  
Ohman Kwon ◽  
Mi-Ok Lee ◽  
Hana Lee ◽  
Ye Seul Son ◽  
...  
Keyword(s):  
Stem Cells ◽  
Human Embryonic Stem Cell ◽  
In Vitro Maturation ◽  
Embryonic Stem ◽  
Generating Functional ◽  
Intestinal Maturation ◽  
Intestinal Organoids ◽  
Hesc Lines

Human intestinal organoids (hIOs), which resemble the human intestine structurally and physiologically, have emerged as a new modality for the study of the molecular and cellular biology of the intestine in vitro. We recently developed an in vitro maturation technique for generating functional hIOs from human pluripotent stem cells (hPSCs). Here, we investigated the function of STAT3 for inducing in vitro maturation of hIOs. This was accompanied by the tyrosine phosphorylation of STAT3, whereas treatment with pharmacological inhibitors of STAT3 suppressed the phosphorylation of STAT3 and the expression of intestinal maturation markers. We generated and characterized STAT3 knockout (KO) human embryonic stem cell (hESC) lines using CRISPR/Cas9-mediated gene editing. We found that STAT3 KO does not affect the differentiation of hESCs into hIOs but rather affects the in vitro maturation of hIOs. STAT3 KO hIOs displayed immature morphologies with decreased size and reduced budding in hIOs even after in vitro maturation. STAT3 KO hIOs showed markedly different profiles from hIOs matured in vitro and human small intestine. Additionally, STAT3 KO hIOs failed to maintain upon in vivo transplantation. This study reveals a core signaling pathway consisting of STAT3 controlling the in vitro maturation of hIOs derived from hPSCs.

scholarly journals Establishment and characterization of two new human embryonic stem cell lines, SYSU-1 and SYSU-2

2007 ◽  
Vol 120 (7) ◽  
pp. 589-594 ◽  
Author(s):  
Guo HUANG ◽  
Wei-qiang LI ◽  
Rui CHEN ◽  
Zhen-guang CHEN ◽  
Xiu-ming ZHANG ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Lines ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Lines

scholarly journals Derivation of new human embryonic stem cell lines reveals rapid epigenetic progression in vitro that can be prevented by chemical modification of chromatin

2011 ◽  
Vol 21 (4) ◽  
pp. 751-764 ◽  
Author(s):  
Silvia V. Diaz Perez ◽  
Rachel Kim ◽  
Ziwei Li ◽  
Victor E. Marquez ◽  
Sanjeet Patel ◽  
...  
Keyword(s):  
Stem Cell ◽  
Chemical Modification ◽  
Embryonic Stem Cell ◽  
Cell Lines ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Lines

scholarly journals Derivation and characterization of four new human embryonic stem cell lines: the Danish experience

2006 ◽  
Vol 12 (1) ◽  
pp. 119-126 ◽  
Author(s):  
Helle Lysdahl ◽  
Anette Gabrielsen ◽  
Stephen L Minger ◽  
Minal J Patel ◽  
Trine Fink ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Lines ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Danish Experience ◽  
Stem Cell Lines

scholarly journals A molecular scheme for improved characterization of human embryonic stem cell lines

BMC Biology ◽  
2006 ◽  
Vol 4 (1) ◽  
Author(s):  
Richard Josephson ◽  
Gregory Sykes ◽  
Ying Liu ◽  
Carol Ording ◽  
Weining Xu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Lines ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Lines ◽  
Molecular Scheme

scholarly journals Derivation and characterization of new Chinese human embryonic stem cell lines with normal and abnormal karyotypes

Cell Research ◽  
2008 ◽  
Vol 18 (S1) ◽  
pp. S33-S33
Author(s):  
Xiaofang Sun ◽  
Yifei Yin ◽  
Yonghua Jiang ◽  
Xiaolin Long ◽  
Hongzi Du ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Lines ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Lines
Sign in / Sign up

Export Citation Format

Share Document