scholarly journals Functionalized Palladium Nanoparticles for Hydrogen Peroxide Biosensor

2011 ◽  
Vol 2011 ◽  
pp. 1-4 ◽  
Author(s):  
H. Baccar ◽  
T. Ktari ◽  
A. Abdelghani
Keyword(s):  
Hydrogen Peroxide ◽  
Cyclic Voltammetry ◽  
Detection Limit ◽  
Horseradish Peroxidase ◽  
Palladium Nanoparticles ◽  
Gold Electrode ◽  
Volume Ratio ◽  
Gold Surface ◽  
Amino Groups ◽  
Limit Detection

We present a comparison between two biosensors for hydrogen peroxide (H2O2) detection. The first biosensor was developed by the immobilization of Horseradish Peroxidase (HRP) enzyme on thiol-modified gold electrode. The second biosensor was developed by the immobilization of cysteamine functionalizing palladium nanoparticles on modified gold surface. The amino groups can be activated with glutaraldehyde for horseradish peroxidase immobilization. The detection of hydrogen peroxide was successfully observed in PBS for both biosensors using the cyclic voltammetry and the chronoamperometry techniques. The results show that the limit detection depends on the large surface-to-volume ratio attained with palladium nanoparticles. The second biosensor presents a better detection limit of 7.5 μM in comparison with the first one which is equal to 75 μM.

A Simple Hydrogen Peroxide Biosensor Based on Catalase Immobilized in Nano-Structural Composite Film

2013 ◽  
Vol 704 ◽  
pp. 258-263
Author(s):  
Zhao Hao Wang ◽  
Han Cui ◽  
Qi Jin Wan ◽  
Nian Jun Yang
Keyword(s):  
Hydrogen Peroxide ◽  
Electron Transfer ◽  
Detection Limit ◽  
Self Assembly ◽  
Nanocomposite Film ◽  
Gold Electrode ◽  
Multilayer Film ◽  
Au Nanoparticles ◽  
Direct Electron Transfer ◽  
Structural Composite

A robust and effective nanocomposite film modified gold electrode based on 2, 3-dimercaptosuccinic acid (DMSA) and Au nanoparticles (AuNPs) was prepared by a method combining self-assembly with underpotential deposition. Direct electron transfer can easily take place between a gold electrode and catalase molecules anchored on AuNPs films. Besides, the CAT/AuNPs/DMSA multilayer film had a relatively rapid and satisfactory response toward H2O2 with a wide linear range from 3.0×106 to 5.86×103 M and a low detection limit of 3.0 μM (S/N = 3). The Michaelis-Menten constant of the immobilized CAT was calculated to be 2.2 mM. The simple CAT/AuNPs/DMSA/Au system can be developed into a H2O2 biosensor.

scholarly journals Electrocatalytical Chemical Sensor for Hydrogen Peroxide

2021 ◽  
Vol 6 (1) ◽  
pp. 1
Author(s):  
Arwa Laroussi ◽  
Noureddine Raouafi ◽  
Vladimir Mirsky
Keyword(s):  
Hydrogen Peroxide ◽  
Cyclic Voltammetry ◽  
Chemical Sensor ◽  
Surface Concentration ◽  
Gold Surface ◽  
Potential Range ◽  
Effective Electron ◽  
Selective Determination ◽  
Relative Standard ◽  
Decomposition Of H2o2

The fast and selective determination of hydrogen peroxide (H2O2) is of importance not only because of strong interest in this widely applied analyte, but also because of the development of enzymatic biosensors for glucose or other metabolites where the sensor for H2O2 can be used as the transducer. Here, we report on an electrocatalytical amperometric sensor for the detection of H2O2. It is a sensor that consists of a gold electrode covered by a self-assembled monolayer (SAM) with immobilized p-benzoquinone. To provide highly stable immobilization of p-benzoquinone at the distance of effective electron tunneling, a new anchor compound—1,3-dimercaptopropan-2-ol—was synthesized and used for preparation of the SAM. Due to two thiol groups binding gold surface, this compound provides high stability of the SAM. The surface concentration of p-benzoquinone obtained from cyclic voltammetry is 2.5 ± 0.2 × 10−10 mol·cm−2. Cyclic voltammetry and chronoamperometry experiments proved that the immobilized benzoquinone exhibited high electrocatalytic activity towards the decomposition of H2O2. Depending on the used potential range, different sensing modes can be realized. For example, one can measure electrochemical response due to the oxidation of H2O2 at anodic potentials, or due to the reduction of oxygen formed during oxidative decomposition of H2O2. Also, amperometric response at fixed potential of +0.4 V vs. Ag/AgCl corresponding to the oxidation of benzoquinone to hydroquinone was studied. The sensor exhibited a linear response over a concentration range of 0.1–2 mM with a low detection limit of 4.24 µM. The reproducibility of three different electrodes prepared was examined at the H2O2 concentration range from 0.1 till 3 mM, which resulted in a relative standard deviation below 4.2%.

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