scholarly journals Effect of Alcohol Structure on the Optimum Condition for Novozym 435-Catalyzed Synthesis of Adipate Esters

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Mohd Basyaruddin Abdul Rahman ◽  
Naz Chaibakhsh ◽  
Mahiran Basri
Keyword(s):  
Maximum Yield ◽  
Candida Antarctica ◽  
Novozym 435 ◽  
Molar Ratio ◽  
Optimum Conditions ◽  
Lipase B ◽  
Tert Butanol ◽  
Ester Yield ◽  
Optimized Conditions ◽  
Central Composite

Immobilized Candida antarctica lipase B, Novozym 435, was used as the biocatalyst in the esterification of adipic acid with four different isomers of butanol (n-butanol, sec-butanol, iso-butanol, and tert-butanol). Optimum conditions for the synthesis of adipate esters were obtained using response surface methodology approach with a four-factor-five-level central composite design concerning important reaction parameters which include time, temperature, substrate molar ratio, and amount of enzyme. Reactions under optimized conditions has yielded a high percentage of esterification (>96%) for n-butanol, iso-butanol, and sec-butanol, indicating that extent of esterification is independent of the alcohol structure for primary and secondary alcohols at the optimum conditions. Minimum reaction time (135 min) for achieving maximum ester yield was obtained for iso-butanol. The required time for attaining maximum yield and also the initial rates in the synthesis of di-n-butyl and di-sec-butyl adipate were nearly the same. Immobilized Candida antarctica lipase B was also capable of esterifying tert-butanol with a maximum yield of 39.1%. The enzyme is highly efficient biocatalyst for the synthesis of adipate esters by offering a simple production process and a high esterification yield.

scholarly journals Galacto-oligosaccharides Production Using Permeabilized Cells of Kluyveromyces marxianus

2010 ◽  
Vol 6 (6) ◽  
Author(s):  
Ana Paula Manera ◽  
Fátima Aparecida de Almeida Costa ◽  
Maria Isabel Rodrigues ◽  
Susana Juliano Kalil ◽  
Francisco Maugeri Filho
Keyword(s):  
Kluyveromyces Marxianus ◽  
Enzyme Concentration ◽  
Human Intestine ◽  
Central Composite Rotatable Design ◽  
Optimum Conditions ◽  
Permeabilized Cells ◽  
Lactose Concentration ◽  
Optimized Conditions ◽  
Central Composite ◽  
Stimulation Of

Galacto-oligosaccharides are non-digestible carbohydrates and are recognized as important prebiotics for than stimulation of the proliferation of lactic acid bacteria and bifidobacteria in the human intestine. GOS can be produced by a transgalactosylation reaction catalysed by ?-galactosidase enzyme, and microorganisms can be used as a source of ?-galactosidase. In this work, a process for producing GOS using permeabilized cells of Kluyveromyces marxianus CCT 7082 was proposed. The effects of the concentrations of lactose and enzyme, temperature and pH were studied using a fractional design followed by a central composite rotatable design. The optimum conditions for galacto-oligosaccharides production were found to be: lactose concentration 500 g/L, enzyme concentration 10 U/mL, 45°C and pH 7.0. Under optimized conditions, the GOS concentration, yield and productivity were 83 g/L, 16.5% and 27.6 g/L.h, respectively.

Comparative Study between Candida antarctica Lipase B and Pseudomonas floroscens as Catalyst for Polycaprolactone Production

2012 ◽  
Vol 626 ◽  
pp. 547-550
Author(s):  
Senthil Kumar Senthil ◽  
M.H. Uzir ◽  
Z. Ahmad
Keyword(s):  
Molecular Weight ◽  
Reaction Time ◽  
Comparative Study ◽  
Ring Opening ◽  
Gradual Increase ◽  
Candida Antarctica ◽  
Novozym 435 ◽  
Lipase B ◽  
Polymerization System ◽  
Effects Of Temperature

The Effects of temperature on ring-opening bulk polymerizations of ε-caprolactone was studied by using two different lipases Novozym 435 (immobilized form of lipase B from Candida antarctica), and Pseudomonas Floroscens as biocatalyst. The polymerization of ε-caprolactone was carried out at 50°C, 60°C, 70°C, 80°C, 90°C, and 100°C. For Novozym 435 the results showed that increasing the reaction time of the polymerization system resulted in an increased rate of monomer consumption and hence increased the molecular weight. For an increase in reaction time the conversion increases steadily and after a gradual increase there is a decrease which is found uniform for all the temperature showing a uniform trend. For a temperature of 70°C and 4 hours molecular weight was found to be 8.4 x 104 daltons which were the highest of all the readings that were obtained. In the copolymerizaton of ε-caprolactone (ε-CL) and δ-valerolactone using Pseudomonas fluorescens lipase at 60°C for 20 days a copolymer with molecular weight of 1.97 x 105 was obtained. Effects of the reaction time and temperature on the copolymerization have been examined.

scholarly journals Efficient Physisorption of Candida Antarctica Lipase B on Polypropylene Beads and Application for Polyester Synthesis

Catalysts ◽  
2018 ◽  
Vol 8 (9) ◽  
pp. 369 ◽  
Author(s):  
Simone Weinberger ◽  
Alessandro Pellis ◽  
James Comerford ◽  
Thomas Farmer ◽  
Georg Guebitz
Keyword(s):  
Molecular Weight ◽  
Reaction Time ◽  
Candida Antarctica ◽  
Candida Antarctica Lipase B ◽  
Novozym 435 ◽  
Polymerization Process ◽  
Candida Antarctica Lipase ◽  
Reaction Products ◽  
Lipase B ◽  
Conversion Rates

In the present work, Candida antarctica lipase B (CaLB) was adsorbed onto polypropylene beads using different reaction conditions, in order to investigate their influence on the immobilization process and the enzyme activity of the preparations in polymerization reactions. In general, lower salt concentrations were more favorable for the binding of enzyme to the carrier. Polymerisation of dimethyl adipate (DMA) and 1,4-butanediol (BDO) was investigated in thin-film systems at 70 °C and at both atmosphere pressure (1000 mbar) and 70 mbar. Conversion rates and molecular masses of the reaction products were compared with reactions catalyzed by CaLB in its commercially available form, known as Novozym 435 (CaLB immobilized on macroporous acrylic resin). The best results according to molecular weight and monomer conversion after 24 h reaction time were obtained with CaLB immobilized in 0.1 M Na2HPO4\NaH2PO4 buffer at pH 8, producing polyesters with 4 kDa at conversion rates of 96% under low pressure conditions. The stability of this preparation was studied in a simulated continuous polymerization process at 70 °C, 70 mbar for 4 h reaction time. The data of this continuous polymerizations show that the preparation produces lower molecular weights at lower conversion rates, but is comparable to the commercial enzyme concerning stability for 10 cycles. However, after 24 h reaction time, using our optimum preparation, higher molecular weight polyesters (4 kDa versus 3.1 kDa) were obtained when compared to Novozym 435.

Direct acylation of flavonoid glycosides with phenolic acids catalysed by Candida antarctica lipase B (Novozym 435®)

2006 ◽  
Vol 39 (6) ◽  
pp. 1236-1241 ◽  
Author(s):  
David E. Stevenson ◽  
Reginald Wibisono ◽  
Dwayne J. Jensen ◽  
Roger A. Stanley ◽  
Janine M. Cooney
Keyword(s):  
Phenolic Acids ◽  
Candida Antarctica ◽  
Candida Antarctica Lipase B ◽  
Novozym 435 ◽  
Flavonoid Glycosides ◽  
Candida Antarctica Lipase ◽  
Lipase B ◽  
Direct Acylation

scholarly journals Optimization of process variables for the biosorption of chromium using Hypnea valentiae

2021 ◽  
Vol 10 (2) ◽  
pp. 107-115
Author(s):  
M. Rajasimman ◽  
K. Murugaiyan
Keyword(s):  
Contact Time ◽  
Red Alga ◽  
Influential Factor ◽  
Quadratic Model ◽  
Batch Experiments ◽  
Process Variables ◽  
Optimum Conditions ◽  
Optimized Conditions ◽  
Central Composite ◽  
Maximum Removal

In this study, Hypnea valentiae, a red alga is used as a sorbent for the removal of chromium from aqueous solutions. The biosorption potential of Hypnea valentiae was investigated in batch experiments. The process parameters were optimized using response surface methodology. Based on the central composite design, quadratic model was developed to correlate the variables to the response. The most influential factor on each experimental design response was identified from the analysis of variance (ANOVA). The optimum conditions for the maximum biosorption of chromium are pH – 2.8, temperature – 48.2oC, sorbent dosage – 5.3 g/L, metal concentration – 103 mg/L and contact time – 27 min. At these optimized conditions the maximum removal was found to be 94.5%.

Combinatorial enzymic synthesis for functional testing of phenolic acid esters catalysed by Candida antarctica lipase B (Novozym 435®)

2007 ◽  
Vol 40 (5) ◽  
pp. 1078-1086 ◽  
Author(s):  
David E. Stevenson ◽  
Shanthi G. Parkar ◽  
Jingli Zhang ◽  
Roger A. Stanley ◽  
Dwayne J. Jensen ◽  
...  
Keyword(s):  
Phenolic Acid ◽  
Candida Antarctica ◽  
Candida Antarctica Lipase B ◽  
Novozym 435 ◽  
Functional Testing ◽  
Candida Antarctica Lipase ◽  
Lipase B ◽  
Enzymic Synthesis ◽  
Acid Esters

scholarly journals Ethyl Butyrate Synthesis Catalyzed by Lipases A and B from Candida antarctica Immobilized onto Magnetic Nanoparticles. Improvement of Biocatalysts’ Performance under Ultrasonic Irradiation

2019 ◽  
Vol 20 (22) ◽  
pp. 5807 ◽  
Author(s):  
Rodolpho R. C. Monteiro ◽  
Davino M. Andrade Neto ◽  
Pierre B. A. Fechine ◽  
Ada A. S. Lopes ◽  
Luciana R. B. Gonçalves ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Ultrasonic Irradiation ◽  
Candida Antarctica ◽  
Ethyl Butyrate ◽  
Molar Ratio ◽  
X Ray Diffraction ◽  
Ultrasound Technology ◽  
Magnetic Nanomaterial ◽  
Co Precipitation ◽  
Optimized Conditions

The synthesis of ethyl butyrate catalyzed by lipases A (CALA) or B (CALB) from Candida antarctica immobilized onto magnetic nanoparticles (MNP), CALA-MNP and CALB-MNP, respectively, is hereby reported. MNPs were prepared by co-precipitation, functionalized with 3-aminopropyltriethoxysilane, activated with glutaraldehyde, and then used as support to immobilize either CALA or CALB (immobilization yield: 100 ± 1.2% and 57.6 ± 3.8%; biocatalysts activities: 198.3 ± 2.7 Up-NPB/g and 52.9 ± 1.7 Up-NPB/g for CALA-MNP and CALB-MNP, respectively). X-ray diffraction and Raman spectroscopy analysis indicated the production of a magnetic nanomaterial with a diameter of 13.0 nm, whereas Fourier-transform infrared spectroscopy indicated functionalization, activation and enzyme immobilization. To determine the optimum conditions for the synthesis, a four-variable Central Composite Design (CCD) (biocatalyst content, molar ratio, temperature and time) was performed. Under optimized conditions (1:1, 45 °C and 6 h), it was possible to achieve 99.2 ± 0.3% of conversion for CALA-MNP (10 mg) and 97.5 ± 0.8% for CALB-MNP (12.5 mg), which retained approximately 80% of their activity after 10 consecutive cycles of esterification. Under ultrasonic irradiation, similar conversions were achieved but at 4 h of incubation, demonstrating the efficiency of ultrasound technology in the enzymatic synthesis of esters.

scholarly journals Chemoenzymatic Epoxidation of Alkenes and Reusability Study of the Phenylacetic Acid

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Emilia Abdulmalek ◽  
Mahashanon Arumugam ◽  
Hanis Nabillah Mizan ◽  
Mohd. Basyaruddin Abdul Rahman ◽  
Mahiran Basri ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Phenylacetic Acid ◽  
Candida Antarctica ◽  
The Other ◽  
Novozym 435 ◽  
Peroxy Acid ◽  
Lipase B ◽  
Reaction Media ◽  
Epoxidation Of Alkenes

Here, we focused on a simple enzymatic epoxidation of alkenes using lipase and phenylacetic acid. The immobilisedCandida antarcticalipase B, Novozym 435 was used to catalyse the formation of peroxy acid instantly from hydrogen peroxide (H2O2) and phenylacetic acid. The peroxy phenylacetic acid generated was then utilised directly forin situoxidation of alkenes. A variety of alkenes were oxidised with this system, resulting in 75–99% yield of the respective epoxides. On the other hand, the phenylacetic acid was recovered from the reaction media and reused for more epoxidation. Interestingly, the waste phenylacetic acid had the ability to be reused for epoxidation of the 1-nonene to 1-nonene oxide, giving an excellent yield of 90%.

scholarly journals Optimization of Alkali Catalyzed Transesterification of Safflower Oil for Production of Biodiesel

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
M. C. Math ◽  
K. N. Chandrashekhara
Keyword(s):  
Reaction Time ◽  
Methyl Ester ◽  
Central Composite Design ◽  
Response Surface ◽  
Rotational Speed ◽  
Catalyst Concentration ◽  
Maximum Yield ◽  
Safflower Oil ◽  
Ester Yield ◽  
Central Composite

The Central Composite Design is used for the optimization of alkaline catalyzed transesterification parameters such as methanol quantity, catalytic concentration, and rotational speed by keeping the temperature and reaction time constant. The Central Composite Design method is employed to get the maximum safflower oil methyl ester yield. The combined effects of catalyst concentration, rotational speed, and molar ratio of alcohol to oil were investigated and optimized using response surface methodology. A statistical model has predicted the maximum yield of safflower oil methyl ester (94.69% volume of oil) parameters such as catalyst concentration (0.6 grams), methanol amount (30 mL), rotational speed (600 rpm), and keeping constant reaction temperature (55°C to 65°C) and reaction time (60 minutes). Experimental maximum yield of 91.66% was obtained at above parameters. XLSTAT is used to generate a linear model to predict the methyl ester yield as a function of methanol quantity, catalyst concentration, and rotational speed by keeping constant reaction temperature (55°C to 65°C) and reaction time (60 minutes). MINITAB is used to draw the 3D response surface plot and 2D contour plot to predict the maximum biodiesel yield.

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