scholarly journals Primitive Sca-1 Positive Bone Marrow HSC in Mouse Model of Aplastic Anemia: A Comparative Study through Flowcytometric Analysis and Scanning Electron Microscopy

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Sumanta Chatterjee ◽  
Pratima Basak ◽  
Prosun Das ◽  
Madhurima Das ◽  
Jacintha Archana Pereira ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Bone Marrow ◽  
Stem Cell ◽  
Aplastic Anemia ◽  
Cell Population ◽  
Receptor Expression ◽  
Stem Cell Population ◽  
Hematopoietic Stem ◽  
Scanning Electron

Self-renewing Hematopoietic Stem Cells (HSCs) are responsible for reconstitution of all blood cell lineages. Sca-1 is the “stem cell antigen” marker used to identify the primitive murine HSC population, the expression of which decreases upon differentiation to other mature cell types.Sca-1+HSCs maintain the bone marrow stem cell pool throughout the life. Aplastic anemia is a disease considered to involve primary stem cell deficiency and is characterized by severe pancytopenia and a decline in healthy blood cell generation system. Studies conducted in our laboratory revealed that the primitiveSca-1+BM-HSCs (bone marrow hematopoietic stem cell) are significantly affected in experimental Aplastic animals pretreated with chemotherapeutic drugs (Busulfan and Cyclophosphamide) and there is increased Caspase-3 activity with consecutive high Annexin-V positivity leading to premature apoptosis in the bone marrow hematopoietic stem cell population in Aplastic condition. TheSca-1bright, that is, “more primitive” BM-HSC population was more affected than the “less primitive” BM-HSCSca-1dim⁡population. The decreased cell population and the receptor expression were directly associated with an empty and deranged marrow microenvironment, which is evident from scanning electron microscopy (SEM). The above experimental evidences hint toward the manipulation of receptor expression for the benefit of cytotherapy by primitive stem cell population in Aplastic anemia cases.

scholarly journals Phenotypic and Functional Changes Induced at the Clonal Level in Hematopoietic Stem Cells After 5-Fluorouracil Treatment

Blood ◽  
1997 ◽  
Vol 89 (10) ◽  
pp. 3596-3606 ◽  
Author(s):  
Troy D. Randall ◽  
Irving L. Weissman
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Bone Marrow ◽  
Stem Cell ◽  
Cell Population ◽  
Stem Cell Population ◽  
Normal Bone Marrow ◽  
Hematopoietic Stem ◽  
Normal Bone

Abstract A significant fraction of hematopoietic stem cells (HSCs) have been shown to be resistant to the effects of cytotoxic agents such as 5-fluorouracil (5-FU), which is thought to eliminate many of the rapidly dividing, more committed progenitors in the bone marrow and to provide a relatively enriched population of the most primitive hematopoietic progenitor cells. Although differences between 5-FU–enriched progenitor populations and those from normal bone marrow have been described, it remained unclear if these differences reflected characteristics of the most primitive stem cells that were revealed by 5-FU, or if there were changes in the stem-cell population itself. Here, we have examined some of the properties of the stem cells in the bone marrow before and after 5-FU treatment and have defined several activation-related changes in the stem-cell population. We found that long-term reconstituting stem cells decrease their expression of the growth factor receptor c-kit by 10-fold and increase their expression of the integrin Mac-1 (CD11b). These changes begin as early as 24 hours after 5-FU treatment and are most pronounced within 2 to 3 days. This activated phenotype of HSCs isolated from 5-FU–treated mice is similar to the phenotype of stem cells found in the fetal liver and to the phenotype of transiently repopulating progenitors in normal bone marrow. We found that cell cycle is induced concomitantly with these physical changes, and within 2 days as many as 29% of the stem-cell population is in the S/G2/M phases of the cell cycle. Furthermore, when examined at a clonal level, we found that 5-FU did not appear to eliminate many of the transient, multipotent progenitors from the bone marrow that were found to be copurified with long-term repopulating, activated stem cells. These results demonstrate the sensitivity of the hematopoietic system to changes in its homeostasis and correlate the expression of several important surface molecules with the activation state of HSCs.

scholarly journals Phenotypic and Functional Changes Induced at the Clonal Level in Hematopoietic Stem Cells After 5-Fluorouracil Treatment

Blood ◽  
1997 ◽  
Vol 89 (10) ◽  
pp. 3596-3606 ◽  
Author(s):  
Troy D. Randall ◽  
Irving L. Weissman
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Bone Marrow ◽  
Stem Cell ◽  
Cell Population ◽  
Stem Cell Population ◽  
Normal Bone Marrow ◽  
Hematopoietic Stem ◽  
Normal Bone

A significant fraction of hematopoietic stem cells (HSCs) have been shown to be resistant to the effects of cytotoxic agents such as 5-fluorouracil (5-FU), which is thought to eliminate many of the rapidly dividing, more committed progenitors in the bone marrow and to provide a relatively enriched population of the most primitive hematopoietic progenitor cells. Although differences between 5-FU–enriched progenitor populations and those from normal bone marrow have been described, it remained unclear if these differences reflected characteristics of the most primitive stem cells that were revealed by 5-FU, or if there were changes in the stem-cell population itself. Here, we have examined some of the properties of the stem cells in the bone marrow before and after 5-FU treatment and have defined several activation-related changes in the stem-cell population. We found that long-term reconstituting stem cells decrease their expression of the growth factor receptor c-kit by 10-fold and increase their expression of the integrin Mac-1 (CD11b). These changes begin as early as 24 hours after 5-FU treatment and are most pronounced within 2 to 3 days. This activated phenotype of HSCs isolated from 5-FU–treated mice is similar to the phenotype of stem cells found in the fetal liver and to the phenotype of transiently repopulating progenitors in normal bone marrow. We found that cell cycle is induced concomitantly with these physical changes, and within 2 days as many as 29% of the stem-cell population is in the S/G2/M phases of the cell cycle. Furthermore, when examined at a clonal level, we found that 5-FU did not appear to eliminate many of the transient, multipotent progenitors from the bone marrow that were found to be copurified with long-term repopulating, activated stem cells. These results demonstrate the sensitivity of the hematopoietic system to changes in its homeostasis and correlate the expression of several important surface molecules with the activation state of HSCs.

scholarly journals Primitive human hematopoietic precursors express Bcl-x but not Bcl-2

Blood ◽  
1995 ◽  
Vol 86 (3) ◽  
pp. 868-876 ◽  
Author(s):  
JR Park ◽  
ID Bernstein ◽  
DM Hockenbery
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Hematopoietic Stem Cell ◽  
Cell Population ◽  
Precursor Cells ◽  
Stem Cell Population ◽  
Term Survival ◽  
Hematopoietic Stem ◽  
Hematopoietic Precursor

Bcl-2 and its homologue, bcl-xL, encode membrane-associated proteins that suppress programmed cell death of hematopoietic cell lines after growth factor withdrawal, and are expressed in hematopoietic precursor cells. To better understand the maintenance of long-term survival in the hematopoietic stem cell population, we evaluated the expression patterns of Bcl-2 and Bcl-x in primitive hematopoietic precursor populations. Hematopoietic precursor cells expressing CD34 (CD34+) and lacking maturation-linked surface antigens (lin-) were isolated from adult human bone marrow using two-color immunofluorescence cell sorting and fractionated on the basis of forward light scatter characteristics into blast-sized and small to medium lymphocyte-sized cell populations. Bcl-2 expression was shown in 78% to 90% of CD34+ lin- blast-sized cells versus less than 10% of small to medium lymphocyte-sized CD34+ lin- cells by immunohistochemical analysis. Small to medium lymphocyte- sized CD34+ lin- cells were further enriched for primitive precursors by selecting cells that lacked expression of CD38 (CD34+ lin- CD38-). In parallel experiments, only 1% to 4% of CD34+ lin- CD38- cells expressed Bcl-2, whereas 45% to 56% of these cells generated colony- forming cells. In contrast, > or = 94% of cells in all bone marrow subpopulations studied expressed Bcl-x protein. Both alternatively spliced bcl-x transcripts, bcl-xL and bcl-xs, were present. Our data show that the most primitive hematopoietic precursors express Bcl-x but not Bcl-2. Thus, the functional bcl-2 homologue, bcl-xL, may be essential for the long-term survival of the hematopoietic stem cell population.

Magnetic cell sorting isolation of therapeutically effective BALB/c mouse bone marrow hematopoietic stem cell population

Biologija ◽  
2008 ◽  
Vol 54 (4) ◽  
pp. 269-273 ◽  
Author(s):  
Vytautas Kašėta ◽  
Gene Biziulevičienė ◽  
Giedrė Ramanauskaitė ◽  
Aida Vaitkuvienė ◽  
Gediminas A. Biziulevičius
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Hematopoietic Stem Cell ◽  
Cell Population ◽  
Cell Sorting ◽  
Stem Cell Population ◽  
Mouse Bone Marrow ◽  
Magnetic Cell Sorting ◽  
Hematopoietic Stem

Primitive human hematopoietic precursors express Bcl-x but not Bcl-2

Blood ◽  
1995 ◽  
Vol 86 (3) ◽  
pp. 868-876 ◽  
Author(s):  
JR Park ◽  
ID Bernstein ◽  
DM Hockenbery
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Hematopoietic Stem Cell ◽  
Cell Population ◽  
Precursor Cells ◽  
Stem Cell Population ◽  
Term Survival ◽  
Hematopoietic Stem ◽  
Hematopoietic Precursor

Abstract Bcl-2 and its homologue, bcl-xL, encode membrane-associated proteins that suppress programmed cell death of hematopoietic cell lines after growth factor withdrawal, and are expressed in hematopoietic precursor cells. To better understand the maintenance of long-term survival in the hematopoietic stem cell population, we evaluated the expression patterns of Bcl-2 and Bcl-x in primitive hematopoietic precursor populations. Hematopoietic precursor cells expressing CD34 (CD34+) and lacking maturation-linked surface antigens (lin-) were isolated from adult human bone marrow using two-color immunofluorescence cell sorting and fractionated on the basis of forward light scatter characteristics into blast-sized and small to medium lymphocyte-sized cell populations. Bcl-2 expression was shown in 78% to 90% of CD34+ lin- blast-sized cells versus less than 10% of small to medium lymphocyte-sized CD34+ lin- cells by immunohistochemical analysis. Small to medium lymphocyte- sized CD34+ lin- cells were further enriched for primitive precursors by selecting cells that lacked expression of CD38 (CD34+ lin- CD38-). In parallel experiments, only 1% to 4% of CD34+ lin- CD38- cells expressed Bcl-2, whereas 45% to 56% of these cells generated colony- forming cells. In contrast, > or = 94% of cells in all bone marrow subpopulations studied expressed Bcl-x protein. Both alternatively spliced bcl-x transcripts, bcl-xL and bcl-xs, were present. Our data show that the most primitive hematopoietic precursors express Bcl-x but not Bcl-2. Thus, the functional bcl-2 homologue, bcl-xL, may be essential for the long-term survival of the hematopoietic stem cell population.

The bulk of the hematopoietic stem cell population is dispensable for murine steady-state and stress hematopoiesis

2017 ◽  
Vol 53 ◽  
pp. S105
Author(s):  
Alexander Gerbaulet ◽  
Kristina Schoedel ◽  
Mina Morcos ◽  
Thomas Zerjatke ◽  
Ingo Roeder ◽  
...  
Keyword(s):  
Stem Cell ◽  
Steady State ◽  
Hematopoietic Stem Cell ◽  
Cell Population ◽  
Stem Cell Population ◽  
Hematopoietic Stem

scholarly journals CD117hi expression identifies a human fetal hematopoietic stem cell population with high proliferation and self-renewal potential

Haematologica ◽  
2019 ◽  
Vol 105 (2) ◽  
pp. e43-e47
Author(s):  
Loïc Maillard ◽  
Sandra Sanfilippo ◽  
Carine Domenech ◽  
Nassima Kasmi ◽  
Laurence Petit ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hematopoietic Stem Cell ◽  
Cell Population ◽  
Stem Cell Population ◽  
Self Renewal ◽  
Hematopoietic Stem

The quantitative trait gene latexin influences the size of the hematopoietic stem cell population in mice

2007 ◽  
Vol 39 (2) ◽  
pp. 178-188 ◽  
Author(s):  
Ying Liang ◽  
Michael Jansen ◽  
Bruce Aronow ◽  
Hartmut Geiger ◽  
Gary Van Zant
Keyword(s):  
Stem Cell ◽  
Hematopoietic Stem Cell ◽  
Cell Population ◽  
Quantitative Trait ◽  
Stem Cell Population ◽  
Hematopoietic Stem ◽  
Quantitative Trait Gene

The bulk of the hematopoietic stem cell population is dispensable for murine steady-state and stress hematopoiesis

2016 ◽  
Vol 44 (9) ◽  
pp. S97
Author(s):  
Kristina Schoedel ◽  
Mina Morcos ◽  
Thomas Zerjatke ◽  
Ingo Roeder ◽  
Tatyana Grinenko ◽  
...  
Keyword(s):  
Stem Cell ◽  
Steady State ◽  
Hematopoietic Stem Cell ◽  
Cell Population ◽  
Stem Cell Population ◽  
Hematopoietic Stem
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