scholarly journals Genome sequence of the Medicago-nodulating Ensifer meliloti commercial inoculant strain RRI128

2014 ◽  
Vol 9 (3) ◽  
pp. 602-613 ◽  
Author(s):  
Wayne Reeve ◽  
Ross Ballard ◽  
Elizabeth Drew ◽  
Rui Tian ◽  
Lambert Bräu ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Ensifer Meliloti ◽  
Commercial Inoculant ◽  
Inoculant Strain

scholarly journals Complete Genome Sequence of Mesorhizobium ciceri bv. biserrulae WSM1497, an Efficient Nitrogen-Fixing Microsymbiont of the Forage Legume Biserrula pelecinus

2017 ◽  
Vol 5 (35) ◽  
Author(s):  
Rachel J. M. Brewer ◽  
Timothy L. Haskett ◽  
Joshua P. Ramsay ◽  
Graham W. O’Hara ◽  
Jason J. Terpolilli
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Forage Legume ◽  
Nitrogen Fixing ◽  
Single Chromosome ◽  
Content Type ◽  
Mesorhizobium Ciceri ◽  
Single Plasmid ◽  
Commercial Inoculant

ABSTRACT We report here the complete genome sequence of Mesorhizobium ciceri bv. biserrulae strain WSM1497, the efficient nitrogen-fixing microsymbiont and commercial inoculant in Australia of the forage legume Biserrula pelecinus. The genome consists of 7.2 Mb distributed across a single chromosome (6.67 Mb) and a single plasmid (0.53 Mb).

scholarly journals Genome sequence of Ensifer meliloti strain WSM1022; a highly effective microsymbiont of the model legume Medicago truncatula A17

2013 ◽  
Vol 9 (2) ◽  
pp. 315-324 ◽  
Author(s):  
Jason Terpolilli ◽  
Yvette Hill ◽  
Rui Tian ◽  
John Howieson ◽  
Lambert Bräu ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Genome Sequence ◽  
Model Legume ◽  
Ensifer Meliloti ◽  
Highly Effective ◽  
Meliloti Strain

Assessment of strains of Bradyrhizobium sp. (Lupinus) for serradellas (Ornithopus spp.)

1996 ◽  
Vol 36 (1) ◽  
pp. 63 ◽  
Author(s):  
RA Ballard
Keyword(s):  
Nitrogen Fixation ◽  
Sandy Soils ◽  
Fold Increase ◽  
Annual Production ◽  
Total Annual Production ◽  
Bradyrhizobium Sp ◽  
Commercial Inoculant ◽  
Inoculant Strain ◽  
Early Production

Several strains of Bradyrhizobium sp. (Lupinus) were compared, in the glasshouse and field, with the Australian commercial inoculant strain, WU42.5, for symbiotic performance on serradella. Strains WSM471 and USDA3709 were more effective in nitrogen fixation (12 and 8% respectively) than WU42.5 across 10 serradella lines, although WU42.5 generally displayed good levels of effectiveness. WSM471 was a more saprophytically competent strain than WU425 in mildly acidic sandy soils, resulting in a 2.8-fold increase in the nodule score of serradella sown 32 cm from a line of bradyrhizobia established the previous year. Although WSM471, when compared with WU42.5, increased the nodulation and early production of an established sward of serradella, it did not improve total annual production of the sward. The possible replacement of WU42.5 with WSM471 in serradella inoculants is discussed.

Genomic panorama of Bradyrhizobium japonicum CPAC 15, a commercial inoculant strain largely established in Brazilian soils and belonging to the same serogroup as USDA 123

2008 ◽  
Vol 40 (11) ◽  
pp. 2743-2753 ◽  
Author(s):  
Leandro Pereira Godoy ◽  
Ana Tereza Ribeiro Vasconcelos ◽  
Ligia Maria Oliveira Chueire ◽  
Rangel Celso Souza ◽  
Marisa Fabiana Nicolás ◽  
...  
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Brazilian Soils ◽  
Commercial Inoculant ◽  
Inoculant Strain

scholarly journals Draft Genome Sequence of Sinorhizobium meliloti AK555

2019 ◽  
Vol 8 (2) ◽  
Author(s):  
Victoria S. Muntyan ◽  
Olga A. Baturina ◽  
Alexey M. Afonin ◽  
Maria E. Cherkasova ◽  
Yuri V. Laktionov ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Sinorhizobium Meliloti ◽  
Biomass Yield ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Salt Tolerant ◽  
Pasture Production ◽  
Content Type ◽  
Legume Seeds ◽  
Inoculant Strain

The inoculation of legume seeds with Sinorhizobium bacteria significantly improves pasture production. Here, we report the draft genome sequence of symbiotically efficient and salt-tolerant Sinorhizobium meliloti inoculant strain AK555, which substantially increases biomass yield of a number of Medicago sativa subsp.

scholarly journals Comparative Genomics across Three Ensifer Species Using a New Complete Genome Sequence of the Medicago Symbiont Sinorhizobium (Ensifer) meliloti WSM1022

2021 ◽  
Vol 9 (12) ◽  
pp. 2428
Author(s):  
Laura Baxter ◽  
Proyash Roy ◽  
Emma Picot ◽  
Jess Watts ◽  
Alex Jones ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
High Efficiency ◽  
Gc Content ◽  
Protein Coding ◽  
Sequencing Technologies ◽  
Ensifer Meliloti ◽  
Meliloti Strain ◽  
Symbiotic Properties

Here, we report an improved and complete genome sequence of Sinorhizobium (Ensifer) meliloti strain WSM1022, a microsymbiont of Medicago species, revealing its tripartite structure. This improved genome sequence was generated combining Illumina and Oxford nanopore sequencing technologies to better understand the symbiotic properties of the bacterium. The 6.75 Mb WSM1022 genome consists of three scaffolds, corresponding to a chromosome (3.70 Mb) and the pSymA (1.38 Mb) and pSymB (1.66 Mb) megaplasmids. The assembly has an average GC content of 62.2% and a mean coverage of 77X. Genome annotation of WSM1022 predicted 6058 protein coding sequences (CDSs), 202 pseudogenes, 9 rRNAs (3 each of 5S, 16S, and 23S), 55 tRNAs, and 4 ncRNAs. We compared the genome of WSM1022 to two other rhizobial strains, closely related Sinorhizobium (Ensifer) meliloti Sm1021 and Sinorhizobium (Ensifer) medicae WSM419. Both WSM1022 and WSM419 species are high-efficiency rhizobial strains when in symbiosis with Medicago truncatula, whereas Sm1021 is ineffective. Our findings report significant genomic differences across the three strains with some similarities between the meliloti strains and some others between the high efficiency strains WSM1022 and WSM419. The addition of this high-quality rhizobial genome sequence in conjunction with comparative analyses will help to unravel the features that make a rhizobial symbiont highly efficient for nitrogen fixation.

Symbiotic characteristics of subspecies of Trifolium subterraneum L.

1968 ◽  
Vol 19 (6) ◽  
pp. 891 ◽  
Author(s):  
AH Gibson ◽  
J Brockwell
Keyword(s):  
Effective Strain ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
The Other ◽  
Bacterial Strains ◽  
Fixed Nitrogen ◽  
Strain Specificity ◽  
Potential Value ◽  
Commercial Inoculant ◽  
Inoculant Strain

An examination was made of the ability of lines of Trifolium subterraneum L. subsp. subterraneum, subsp. yanninicum Katzn. et Morley, and subsp. brachycalycinum Katzn. et Morley to nodulate and fix nitrogen with strains of .Rlzizobium trifolii isolated from habitats dominated by each of the subspecies.Host line x bacterial strain specificity was demonstrated at two levels: (a) some strains nodulated one, or two, host lines ineffectively, yet formed an effective symbiosis with the other host lines; (b) among the symbiotic combinations classed as effective, the degree of effectiveness of the strains was dependent on the host lines. It was found that the lines of a subspecies were not more effectively nodulated by the bacterial strains isolated from a habitat in which that subspecies was the predominant subterranean clover, than they were by strains isolated from habitats in which the other subspecies predominated. For example, lines of T. subterraneum subsp. yanninicum fixed nitrogen as well with strains of R. trifolii isolated from habitats of subsp. subterraneum or subsp. brachycalycinum as they did with strains isolated from its own habitat. Similarly, there was little evidence that the strains from the habitat in which a subspecies predominated were more effective in their symbiosis with the lines of that subspecies than they were with the lines of the other subspecies. The only exception to this latter generalization was that the strains isolated from the subsp. brachycalycinum habitat were slightly more effective with subsp. brachycalycinum than with subspp. yanninicum or subterraneum. Groupings of the strains based on their response with the lines of any subspecies, or with the lines of all subspecies combined, bore no relation to groupings based on the origin of the strains. It was concluded that it was not possible to predict the effectiveness of any symbiotic combination from a consideration of the taxonomy of the host or the habitat from which the bacterium was isolated. The feasibility of using a standard host line for the screening of the general effectiveness of all strains of potential value in subterranean clover inoculants is discussed. The commercial inoculant strain, TA1, was not the most effective strain with all lines, and overall, ranked fifth among the strains examined.

Nodulation and growth of pasture legumes with naturalised soil rhizobia.2. Balansa clover (Trifolium michelianum Savi)

2002 ◽  
Vol 42 (7) ◽  
pp. 939 ◽  
Author(s):  
R. A. Ballard ◽  
A. D. Craig ◽  
N. Charman
Keyword(s):  
Rhizobium Leguminosarum ◽  
Widespread Occurrence ◽  
Pasture Legumes ◽  
Cultivar Selection ◽  
Eastern Australia ◽  
Commercial Cultivars ◽  
Commercial Inoculant ◽  
Inoculant Strain ◽  
History Of ◽  
Trifolium Michelianum

Balansa clover (Trifolium michelianum Savi) is grown widely across southern and eastern Australia. The presence of suitable rhizobia (Rhizobium leguminosarum bv. trifolii) for this species of clover, and their ability to form an effective symbiosis, was assessed in a survey of 43 soils collected from across Australia. The effectiveness of the soil rhizobia is compared with that of strains WU95 and WSM409. The study confirmed the widespread occurrence of clover rhizobia in Australian soils. No clover rhizobia were detected in 6 of the 43 soils and this was probably associated with a history of clover absence at these soil locations. Thirty of the soils contained more than 500 rhizobia per gram. These rhizobia varied from highly effective for soil T2 (128% of WSM409) through to ineffective for soil V9 (22% of WSM409). Few soil inoculants were more effective than strain WSM409. Commercial inoculant strain WSM409 was generally more effective than former inoculant strain WU95.The suboptimal performance of the rhizobia in many of the soils indicates that there is an opportunity to improve the level of nitrogen fixation by balansa clover in the field. Bearing in mind the difficulties associated with the introduction and persistence of applied inoculant strains, cultivar selection is considered as an approach to improve the symbiosis. However, this study indicated little variation in the performance of the 3 commercial cultivars, Frontier, Paradana and Bolta, in this regard.

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