scholarly journals Purification and Characterization of Endo-xyloglucan Transferase, a Key Enzyme Responsible for Plant Cell-wall Loosening

1993 ◽  
Vol 5 (22) ◽  
pp. 145-146
Author(s):  
Y. Kato
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Purification And Characterization ◽  
Cell Wall Loosening ◽  
Key Enzyme ◽  
Wall Loosening

scholarly journals Functional characterization of genes mediating cell wall metabolism and responses to plant cell wall integrity impairment

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Timo Engelsdorf ◽  
Lars Kjaer ◽  
Nora Gigli-Bisceglia ◽  
Lauri Vaahtera ◽  
Stefan Bauer ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Functional Characterization ◽  
Cell Wall Integrity ◽  
Cell Wall Metabolism

scholarly journals Correction to: Functional characterization of genes mediating cell wall metabolism and responses to plant cell wall integrity impairment

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Timo Engelsdorf ◽  
Lars Kjaer ◽  
Nora Gigli-Bisceglia ◽  
Lauri Vaahtera ◽  
Stefan Bauer ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Functional Characterization ◽  
Cell Wall Integrity ◽  
Cell Wall Metabolism

Multi-scale visualization and characterization of lignocellulosic plant cell wall deconstruction during thermochemical pretreatment

2011 ◽  
Vol 4 (3) ◽  
pp. 973 ◽  
Author(s):  
Shishir P. S. Chundawat ◽  
Bryon S. Donohoe ◽  
Leonardo da Costa Sousa ◽  
Thomas Elder ◽  
Umesh P. Agarwal ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Thermochemical Pretreatment ◽  
Multi Scale

Characterization of a thermostable endoglucanase from Cellulomonas fimi ATCC484

2018 ◽  
Vol 96 (1) ◽  
pp. 68-76 ◽  
Author(s):  
Hirak Saxena ◽  
Bryan Hsu ◽  
Marc de Asis ◽  
Mirko Zierke ◽  
Lyann Sim ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Hydrolytic Activity ◽  
Carbohydrate Binding ◽  
Cell Wall Polysaccharides ◽  
Cellulomonas Fimi ◽  
Thermal Denaturation Temperature ◽  
Mesophilic Bacterium

Bacteria in the genus Cellulomonas are well known as secretors of a variety of mesophilic carbohydrate degrading enzymes (e.g., cellulases and hemicellulases), active against plant cell wall polysaccharides. Recent proteomic analysis of the mesophilic bacterium Cellulomonas fimi ATCC484 revealed uncharacterized enzymes for the hydrolysis of plant cell wall biomass. Celf_1230 (CfCel6C), a secreted protein of Cellulomonas fimi ATCC484, is a novel member of the GH6 family of cellulases that could be successfully expressed in Escherichia coli. This enzyme displayed very little enzymatic/hydrolytic activity at 30 °C, but showed an optimal activity around 65 °C, and exhibited a thermal denaturation temperature of 74 °C. In addition, it also strongly bound to filter paper despite having no recognizable carbohydrate binding module. Our experiments show that CfCel6C is a thermostable endoglucanase with activity on a variety of β-glucans produced by an organism that struggles to grow above 30 °C.

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